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The map label for this gene is recG [H]

Identifier: 28210919

GI number: 28210919

Start: 1319119

End: 1321170

Strand: Direct

Name: recG [H]

Synonym: CTC01231

Alternate gene names: 28210919

Gene position: 1319119-1321170 (Clockwise)

Preceding gene: 28210918

Following gene: 28210920

Centisome position: 47.12

GC content: 27.44

Gene sequence:

>2052_bases
ATGGATATATATAATAGTGTAGGTGTATTAAAAGGAGTAGGACCTAAAACTTTAGAAAACTTAAATAAATGCGGTATATT
TACAATAATAGATTTATTATTATATTTTCCAAGAGATTATAAAATTATAAAAACTTGTACTAGTTTAAAAGATGCTTTAG
AGAAACAAGAAATAATTATAACTGCAGAAGTTTTAGAAATAAAAAGAGATGTACGAACTAGATCTGGTAAAGTGATATCC
ACAATAATATTACAAAATGAAGAAGAGAGAATAAAATGTAGGTGGTTTAATCAACCCTACATTAAAAATAGATTTCGAAT
AGGTAAGAAGTACACTCTTAATGGAAAATTAGATGTATACAAAGGAGAAAAATTAATTATAAATCCCAAAACAGTTGAGA
AAAATGCTGAAGCAACTAATTGTACAAAAGAAATAGTAATACCTATATATCCTTTAAGAGAAGGTGTAAAGAATAATACT
TTAGTAAAATTAATAAAAGATGTTCTAGATAAATTAATAATAGATGAAAATTTACCTACATGGATTATGAAAAAGTATAA
TTTTTGTTCTTTAGATGAAAGTATTAGAGCTATTCATAACCCAATTAGTGAATCTCAGTTAGAATTTGCTAAAAGAAGAT
TAAAGTTTCAGGAACTTTTTACTTATTCATTAAAAATAGCCCTATTAAAAGAAAGTAGAAAACAAAGTAATAAAGGTATA
TCCTTTAAAATATATAAAGAGGAAATAAATAAACTGCAAGATAAATTAAATTTTCAGCTTACTAAGGCACAAAAAATAGT
ACTAGAAGAAATTTTAAAGGATCAAAATAAAAATATTTCAATGAATAGATTAGTACAAGGGGATGTTGGATCAGGCAAAA
CTATACTTGCTATAATTACACTACTTAATGTGGCTATGAATGGATATCAAGGAGTTTTAATGGTACCAACAGAAATATTA
GCACAACAACATTTTATAGAAATTAAAAATTTACTTTCAGAATTTAATATAAGAGTAGAACTTTTATGTGGAAGCACATC
AAATAAGGAAAAAAGCAATATAAAGGCAGCACTAAAGGGTGGAGAAATAGACATAATAATAGGAACTCATGCTTTAATAG
AAGATGATGTAGAATTTGAAAAATTGGGAATTGTAATAACGGATGAACAGCATAGATTTGGAGTTATGCAGAGAAATAAA
TTATTTACAAAGGGTGAAGGTGCAGATATATTAGTAATGACTGCAACACCAATACCAAGAACCTTGAGTCTATACTTATA
TGGAGATCTAGATATTTCTATAATAGACGAATTGCCTCCAGGCAGACAAAAGGTAGATACTTATTTTATAAATGAAAAAA
AACGGGATAGGATATACTCCTTTGCGTTAAAAGAAATTAATGAAGGAAGACAGGTATATGTAGTTTGTCCTCTAGTAGAG
GAAAATGATCAATTGGAGTTAACTTCTGTGGAGGAACTTTATAAGGATTTAAAAGAAAACTATTTTAATGAAATAGAAAT
GGAGATACTACATGGTAAAATGACTCCAAAAGAAAAAAATGATATTATGCTGAGATTTAAAAATAATGAAATAAAAATAT
TAATATCCACAACTGTAATAGAAGTAGGTATTAATGTCCCTAATGCTACTATTATGGTGGTAGAAGGAGCGGAGAGATTT
GGATTGGCACAACTCCATCAGCTAAGAGGAAGAGTTGGAAGAGGTAGTGAGAAGTCCTACTGTATTCTAATGGGAAATAT
TAAAAATATGAACACAAGAAAAAGAATGGAAACCTTAGTAAAAAGTAATGATGGATTCTTTATTGCGGAACAGGATTTAA
AAATAAGAGGTAGTGGAGAACTATTTGGATTTAGACAACATGGAGAAGAAAATTTAATTCTATCTAATCCTATAGAGGAT
ATAGAAATACTTAAAATAGCCCATAATGAATCACAAGAACTATTAAAAAGTAATAAAAAAGAGGATTTAAAAATTCTTGG
ATATTTAGGCGAAAAGATAGATAAACACAAAGAATATATTTGTTTTAATTAA

Upstream 100 bases:

>100_bases
AGGAAAACAGCCATTATATTATTTCATTATCTCTGTTGAATAAAAAAAGCCATTTGGCTTTTTTTATTTAATTAGAAAAA
TAGGTGTGGAGTGATAACAC

Downstream 100 bases:

>100_bases
ATGTAATTTTTTATAAAGACTTTGTGAAAAAATAATAAATATGATAAAATTATAACAAAAACTACATAGTTAATAGTCAG
GAGGATAAAAATGAGAATAA

Product: ATP-dependent DNA helicase recG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MDIYNSVGVLKGVGPKTLENLNKCGIFTIIDLLLYFPRDYKIIKTCTSLKDALEKQEIIITAEVLEIKRDVRTRSGKVIS
TIILQNEEERIKCRWFNQPYIKNRFRIGKKYTLNGKLDVYKGEKLIINPKTVEKNAEATNCTKEIVIPIYPLREGVKNNT
LVKLIKDVLDKLIIDENLPTWIMKKYNFCSLDESIRAIHNPISESQLEFAKRRLKFQELFTYSLKIALLKESRKQSNKGI
SFKIYKEEINKLQDKLNFQLTKAQKIVLEEILKDQNKNISMNRLVQGDVGSGKTILAIITLLNVAMNGYQGVLMVPTEIL
AQQHFIEIKNLLSEFNIRVELLCGSTSNKEKSNIKAALKGGEIDIIIGTHALIEDDVEFEKLGIVITDEQHRFGVMQRNK
LFTKGEGADILVMTATPIPRTLSLYLYGDLDISIIDELPPGRQKVDTYFINEKKRDRIYSFALKEINEGRQVYVVCPLVE
ENDQLELTSVEELYKDLKENYFNEIEMEILHGKMTPKEKNDIMLRFKNNEIKILISTTVIEVGINVPNATIMVVEGAERF
GLAQLHQLRGRVGRGSEKSYCILMGNIKNMNTRKRMETLVKSNDGFFIAEQDLKIRGSGELFGFRQHGEENLILSNPIED
IEILKIAHNESQELLKSNKKEDLKILGYLGEKIDKHKEYICFN

Sequences:

>Translated_683_residues
MDIYNSVGVLKGVGPKTLENLNKCGIFTIIDLLLYFPRDYKIIKTCTSLKDALEKQEIIITAEVLEIKRDVRTRSGKVIS
TIILQNEEERIKCRWFNQPYIKNRFRIGKKYTLNGKLDVYKGEKLIINPKTVEKNAEATNCTKEIVIPIYPLREGVKNNT
LVKLIKDVLDKLIIDENLPTWIMKKYNFCSLDESIRAIHNPISESQLEFAKRRLKFQELFTYSLKIALLKESRKQSNKGI
SFKIYKEEINKLQDKLNFQLTKAQKIVLEEILKDQNKNISMNRLVQGDVGSGKTILAIITLLNVAMNGYQGVLMVPTEIL
AQQHFIEIKNLLSEFNIRVELLCGSTSNKEKSNIKAALKGGEIDIIIGTHALIEDDVEFEKLGIVITDEQHRFGVMQRNK
LFTKGEGADILVMTATPIPRTLSLYLYGDLDISIIDELPPGRQKVDTYFINEKKRDRIYSFALKEINEGRQVYVVCPLVE
ENDQLELTSVEELYKDLKENYFNEIEMEILHGKMTPKEKNDIMLRFKNNEIKILISTTVIEVGINVPNATIMVVEGAERF
GLAQLHQLRGRVGRGSEKSYCILMGNIKNMNTRKRMETLVKSNDGFFIAEQDLKIRGSGELFGFRQHGEENLILSNPIED
IEILKIAHNESQELLKSNKKEDLKILGYLGEKIDKHKEYICFN
>Mature_683_residues
MDIYNSVGVLKGVGPKTLENLNKCGIFTIIDLLLYFPRDYKIIKTCTSLKDALEKQEIIITAEVLEIKRDVRTRSGKVIS
TIILQNEEERIKCRWFNQPYIKNRFRIGKKYTLNGKLDVYKGEKLIINPKTVEKNAEATNCTKEIVIPIYPLREGVKNNT
LVKLIKDVLDKLIIDENLPTWIMKKYNFCSLDESIRAIHNPISESQLEFAKRRLKFQELFTYSLKIALLKESRKQSNKGI
SFKIYKEEINKLQDKLNFQLTKAQKIVLEEILKDQNKNISMNRLVQGDVGSGKTILAIITLLNVAMNGYQGVLMVPTEIL
AQQHFIEIKNLLSEFNIRVELLCGSTSNKEKSNIKAALKGGEIDIIIGTHALIEDDVEFEKLGIVITDEQHRFGVMQRNK
LFTKGEGADILVMTATPIPRTLSLYLYGDLDISIIDELPPGRQKVDTYFINEKKRDRIYSFALKEINEGRQVYVVCPLVE
ENDQLELTSVEELYKDLKENYFNEIEMEILHGKMTPKEKNDIMLRFKNNEIKILISTTVIEVGINVPNATIMVVEGAERF
GLAQLHQLRGRVGRGSEKSYCILMGNIKNMNTRKRMETLVKSNDGFFIAEQDLKIRGSGELFGFRQHGEENLILSNPIED
IEILKIAHNESQELLKSNKKEDLKILGYLGEKIDKHKEYICFN

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=651, Percent_Identity=38.0952380952381, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787357, Length=441, Percent_Identity=36.9614512471655, Blast_Score=270, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78464; Mature: 78464

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIYNSVGVLKGVGPKTLENLNKCGIFTIIDLLLYFPRDYKIIKTCTSLKDALEKQEIII
CCCCCCCCHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
TAEVLEIKRDVRTRSGKVISTIILQNEEERIKCRWFNQPYIKNRFRIGKKYTLNGKLDVY
EHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHCCCCEEEECCEEEEE
KGEKLIINPKTVEKNAEATNCTKEIVIPIYPLREGVKNNTLVKLIKDVLDKLIIDENLPT
ECCEEEECCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
WIMKKYNFCSLDESIRAIHNPISESQLEFAKRRLKFQELFTYSLKIALLKESRKQSNKGI
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCC
SFKIYKEEINKLQDKLNFQLTKAQKIVLEEILKDQNKNISMNRLVQGDVGSGKTILAIIT
EEEEEHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCHHHHHHH
LLNVAMNGYQGVLMVPTEILAQQHFIEIKNLLSEFNIRVELLCGSTSNKEKSNIKAALKG
HHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHCCCEEECC
GEIDIIIGTHALIEDDVEFEKLGIVITDEQHRFGVMQRNKLFTKGEGADILVMTATPIPR
CEEEEEEECHHHCCCCCCEEHEEEEEECCCHHCCCHHCCCEEECCCCCEEEEEECCCCCC
TLSLYLYGDLDISIIDELPPGRQKVDTYFINEKKRDRIYSFALKEINEGRQVYVVCPLVE
EEEEEEEECCCEEEECCCCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
ENDQLELTSVEELYKDLKENYFNEIEMEILHGKMTPKEKNDIMLRFKNNEIKILISTTVI
CCCCEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEE
EVGINVPNATIMVVEGAERFGLAQLHQLRGRVGRGSEKSYCILMGNIKNMNTRKRMETLV
EEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
KSNDGFFIAEQDLKIRGSGELFGFRQHGEENLILSNPIEDIEILKIAHNESQELLKSNKK
HCCCCEEEEECCEEEECCCCEEEECCCCCCCEEECCCCCCEEEEEEECCCHHHHHHCCCC
EDLKILGYLGEKIDKHKEYICFN
CHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MDIYNSVGVLKGVGPKTLENLNKCGIFTIIDLLLYFPRDYKIIKTCTSLKDALEKQEIII
CCCCCCCCHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
TAEVLEIKRDVRTRSGKVISTIILQNEEERIKCRWFNQPYIKNRFRIGKKYTLNGKLDVY
EHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHCCCCEEEECCEEEEE
KGEKLIINPKTVEKNAEATNCTKEIVIPIYPLREGVKNNTLVKLIKDVLDKLIIDENLPT
ECCEEEECCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
WIMKKYNFCSLDESIRAIHNPISESQLEFAKRRLKFQELFTYSLKIALLKESRKQSNKGI
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCC
SFKIYKEEINKLQDKLNFQLTKAQKIVLEEILKDQNKNISMNRLVQGDVGSGKTILAIIT
EEEEEHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCHHHHHHH
LLNVAMNGYQGVLMVPTEILAQQHFIEIKNLLSEFNIRVELLCGSTSNKEKSNIKAALKG
HHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHCCCEEECC
GEIDIIIGTHALIEDDVEFEKLGIVITDEQHRFGVMQRNKLFTKGEGADILVMTATPIPR
CEEEEEEECHHHCCCCCCEEHEEEEEECCCHHCCCHHCCCEEECCCCCEEEEEECCCCCC
TLSLYLYGDLDISIIDELPPGRQKVDTYFINEKKRDRIYSFALKEINEGRQVYVVCPLVE
EEEEEEEECCCEEEECCCCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
ENDQLELTSVEELYKDLKENYFNEIEMEILHGKMTPKEKNDIMLRFKNNEIKILISTTVI
CCCCEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEE
EVGINVPNATIMVVEGAERFGLAQLHQLRGRVGRGSEKSYCILMGNIKNMNTRKRMETLV
EEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
KSNDGFFIAEQDLKIRGSGELFGFRQHGEENLILSNPIEDIEILKIAHNESQELLKSNKK
HCCCCEEEEECCEEEECCCCEEEECCCCCCCEEECCCCCCEEEEEEECCCHHHHHHCCCC
EDLKILGYLGEKIDKHKEYICFN
CHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830261; 11463916 [H]