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The map label for this gene is def

Identifier: 28210907

GI number: 28210907

Start: 1307078

End: 1307530

Strand: Direct

Name: def

Synonym: CTC01219

Alternate gene names: 28210907

Gene position: 1307078-1307530 (Clockwise)

Preceding gene: 28210906

Following gene: 28210908

Centisome position: 46.69

GC content: 32.67

Gene sequence:

>453_bases
ATGGCAATAAGGAATTTAAGATTTTTAGGTGACGAATTATTAAGAAAGAAAAGTAGAAGAGTAGAGAAAATAGATGACAG
AATTCAAGCATTATTAGATGATATGCTAGACACCATGTATGAGAACAATGGTGTAGGATTAGCAGCACCTCAGGTTGGAA
TATTAAAAAGAGTAGTAGTTATAGATATTGGAGAAGGACCTTTATTTTTAATAAATCCAGAAATTATAGAACAAGAGGGA
TCTTATATAGAGCAAGAAGGATGTTTAAGTGTTCCTGGTAGACAAGGGGAAGTAGAAAGACCTTATAGGGTAAAAGTCAA
GGCACAAAATAGAGATGGAAAGGAAATAATAGTTGAAGGAGAGGAATTTTTAGCAAAGGCTCTATGCCATGAAATAGATC
ATTTAAATGGAGTTCTATTTGTAGATAAAATAATAGATGTGAAGGGGGAATAA

Upstream 100 bases:

>100_bases
AAAGTATATGAAAACTTAAAGGATGTATATAATGATATAAAAGTTAGCATAGATATAAATCCTACAAGCTTAATGTAAAT
TTATTTGGAGGTAGTTTAAT

Downstream 100 bases:

>100_bases
TTTGATGAAAATAGTATTTATGGGCACACCTGAATTTGCAGTACCATCTTTAGAAAGGCTTATTAAAGAATTTGGTGTTA
AAGCTGTTTTCACTCAACCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MAIRNLRFLGDELLRKKSRRVEKIDDRIQALLDDMLDTMYENNGVGLAAPQVGILKRVVVIDIGEGPLFLINPEIIEQEG
SYIEQEGCLSVPGRQGEVERPYRVKVKAQNRDGKEIIVEGEEFLAKALCHEIDHLNGVLFVDKIIDVKGE

Sequences:

>Translated_150_residues
MAIRNLRFLGDELLRKKSRRVEKIDDRIQALLDDMLDTMYENNGVGLAAPQVGILKRVVVIDIGEGPLFLINPEIIEQEG
SYIEQEGCLSVPGRQGEVERPYRVKVKAQNRDGKEIIVEGEEFLAKALCHEIDHLNGVLFVDKIIDVKGE
>Mature_149_residues
AIRNLRFLGDELLRKKSRRVEKIDDRIQALLDDMLDTMYENNGVGLAAPQVGILKRVVVIDIGEGPLFLINPEIIEQEGS
YIEQEGCLSVPGRQGEVERPYRVKVKAQNRDGKEIIVEGEEFLAKALCHEIDHLNGVLFVDKIIDVKGE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=27.0440251572327, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=42.483660130719, Blast_Score=122, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=32.9268292682927, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CLOTE (Q895Q2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781851.1
- ProteinModelPortal:   Q895Q2
- SMR:   Q895Q2
- GeneID:   1059549
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01219
- NMPDR:   fig|212717.1.peg.1073
- HOGENOM:   HBG665227
- OMA:   EFLAIVM
- PhylomeDB:   Q895Q2
- ProtClustDB:   PRK00150
- BioCyc:   CTET212717:CTC_01219-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 16925; Mature: 16794

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRNLRFLGDELLRKKSRRVEKIDDRIQALLDDMLDTMYENNGVGLAAPQVGILKRVVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHEEEEE
IDIGEGPLFLINPEIIEQEGSYIEQEGCLSVPGRQGEVERPYRVKVKAQNRDGKEIIVEG
EECCCCCEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEC
EEFLAKALCHEIDHLNGVLFVDKIIDVKGE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure 
AIRNLRFLGDELLRKKSRRVEKIDDRIQALLDDMLDTMYENNGVGLAAPQVGILKRVVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHEEEEE
IDIGEGPLFLINPEIIEQEGSYIEQEGCLSVPGRQGEVERPYRVKVKAQNRDGKEIIVEG
EECCCCCEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEC
EEFLAKALCHEIDHLNGVLFVDKIIDVKGE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129