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The map label for this gene is priA [H]

Identifier: 28210906

GI number: 28210906

Start: 1304821

End: 1307055

Strand: Direct

Name: priA [H]

Synonym: CTC01218

Alternate gene names: 28210906

Gene position: 1304821-1307055 (Clockwise)

Preceding gene: 28210905

Following gene: 28210907

Centisome position: 46.61

GC content: 26.67

Gene sequence:

>2235_bases
TTGTTTAATGATAAAAAGGGTGATAAAGTGTATAGATATGCAGGTGTAGTTGTAAACAGTAATTCAATTCAATTGGATAG
AATTTTTACTTATAAAATATCGGAAGAACTTGCACTAAAGATTAAAGTTGGAAATAGAGTGTTAGTTCCTTTTGGTATGG
GTAATAAAAAAATAGATGCTTTTGTAATGAATTTATATGAAGACATAGAAAATAAAGAAAATATAAAAATAAAAGAAATA
ATTAAGATATATGAAAAATTTCCTGTATTATCTTACAAGGATATACGTATTGTAGAATATATGAGAAAACATTGTTTATC
TACTTATATGGAAGGGATAAGGACTATATTACCCTCTATACTTTTTAAAGGAGTTAAAAAGAAAAAAGAAAATCTAATAT
ATATAGGTAAATCTCTAGAGGGCAGGCATAATAAAGAACCATACATAAATATATACAATATAGTAAAAGAAAATAGTGGA
AAGTTTAATAAAACTGCCTTAAGCAAAAAATTTGATTTATCTTTATCTTCCATAAACACATTGCTAAAATATGAATTTTT
AACTCAAGAAGAAAAAGTTATAAATAGATATAATGATAAAATTTATAAAGGTTATGAAGAAAAAGTTTTAAATGATGAAC
AAAGAAGTGCTATAGAAAAAATAATGAATTCAGAAAAAAATATGTTTCTAATAAAAGGTGTTACTGGTAGTGGTAAAACG
GAAATATATATGAATTTAGTAAAGGAAATGCTAAAAGAAGGTAAGGATTCTATTATATTAGTTCCAGAAATAGCTCTAAC
ACCTCAAATGATAGAAAGATTTAAAGGAAGATTTGGAAAGGATATTACTCTTTTTCATAGTAGACTTTCTGATGGAGAAA
AATATGATGAATGGGTAAGGGTTAAAAAAGGAGAAGTTAAAATTGCTATAGGACCGAGATCAGCAATTTTTTTACCTTTT
AACAATTTAGGACTAATAGTAATAGATGAAGAACATGAAACTAGCTATAAATCTGATAGTGATCCTAAATATAATGCAAG
GGACATAGGGAGAATAAAAAGTGAATTGTATAATTGTAAACTAGTACTGGGAACAGCTACCCCATCCATAGAAAGTTACT
ATGCCTCTAAAATAGGGGAACTAGAACTTATAGAACTTTTCAATAGAACCGATGGTGCAGCTATGCCAAAGATTGAAATT
GTGGATATGAGAGAGGAATTAATAAGTAATAACAAATCTATTTTTAGTAAAAAACTTTTTGAAGATATAGAAGAAAGATT
AAATAAAAAAGAACAAATAATATTATTCCTAAATAGAAGGGGTTTTTCCACATTTGTTTCCTGTAGGAAATGTGGTTATG
TTTTTAAATGTAATAACTGTGATATAGCCCTTACTTATCATAATCGAAATTCTTTTTTGATATGTCATTATTGTGGTAAA
AGAGAAAGGGTTTTAAAAACTTGTCCTAAATGTGGTAGTTCCTATGTAAAATATTTTGGAGCAGGAACAGAAAGAATAGA
AAAAGAAGTGCACAAATATTTTCCTAAAGCAAGAACTTTAAGAATGGATTTTGATACCACAAGAAAGAAGAACTCTCATG
AAAGAATATATGAAACCTTTAAAAATGGTAAGGCAGATATTTTAATAGGTACGCAAATGATAGCTAAAGGGTTAGATTTT
GAAAATGTAACTTTAGTTGGAGTAATTGCAGCTGACCTATCTCTTAATATACCAGACTATAAAGCTACAGAAAGAACTTT
CCAACTTATAACGCAAGTATCAGGAAGAGCAGGTAGAGGTAAGAAAGAAGGAAAAGTTATAGTTCAAACCTATAGTCCAG
ATAATTATAGCATAATATATTCTGCTAATAATGAATATGATAAATTTTATAAAGAAGAGGTAGAATACAGAAGATTAATG
GACTATCCTCCTTTTACAGAAATTATGCTTATAAATTTAAGTAGTAAAAATGAGGATCTATTAATAAAAAGTATACAAAA
TGTTGGTATAATATTAAAAGATACTTTAAAAAATAATGATAAAATAAATGTACTAGGACCATGTCCTTGTGAAATATCTA
AAATAAAAAATAATTATAGATGGCAGATAACCTTAAAGGGAATTATAAGCCATGAGTTAAAACATATTATAAAAAACAAA
GTATATGAAAACTTAAAGGATGTATATAATGATATAAAAGTTAGCATAGATATAAATCCTACAAGCTTAATGTAA

Upstream 100 bases:

>100_bases
AGTAGCAAGAGAAATTTTTGATTTAATATTAAAAGAGAAATTCTAAAAAAGTGCAGGGCACTTTTTTAGTGAATTTAAAA
GGGGCTAAAGTAAGCCTCTT

Downstream 100 bases:

>100_bases
ATTTATTTGGAGGTAGTTTAATATGGCAATAAGGAATTTAAGATTTTTAGGTGACGAATTATTAAGAAAGAAAAGTAGAA
GAGTAGAGAAAATAGATGAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MFNDKKGDKVYRYAGVVVNSNSIQLDRIFTYKISEELALKIKVGNRVLVPFGMGNKKIDAFVMNLYEDIENKENIKIKEI
IKIYEKFPVLSYKDIRIVEYMRKHCLSTYMEGIRTILPSILFKGVKKKKENLIYIGKSLEGRHNKEPYINIYNIVKENSG
KFNKTALSKKFDLSLSSINTLLKYEFLTQEEKVINRYNDKIYKGYEEKVLNDEQRSAIEKIMNSEKNMFLIKGVTGSGKT
EIYMNLVKEMLKEGKDSIILVPEIALTPQMIERFKGRFGKDITLFHSRLSDGEKYDEWVRVKKGEVKIAIGPRSAIFLPF
NNLGLIVIDEEHETSYKSDSDPKYNARDIGRIKSELYNCKLVLGTATPSIESYYASKIGELELIELFNRTDGAAMPKIEI
VDMREELISNNKSIFSKKLFEDIEERLNKKEQIILFLNRRGFSTFVSCRKCGYVFKCNNCDIALTYHNRNSFLICHYCGK
RERVLKTCPKCGSSYVKYFGAGTERIEKEVHKYFPKARTLRMDFDTTRKKNSHERIYETFKNGKADILIGTQMIAKGLDF
ENVTLVGVIAADLSLNIPDYKATERTFQLITQVSGRAGRGKKEGKVIVQTYSPDNYSIIYSANNEYDKFYKEEVEYRRLM
DYPPFTEIMLINLSSKNEDLLIKSIQNVGIILKDTLKNNDKINVLGPCPCEISKIKNNYRWQITLKGIISHELKHIIKNK
VYENLKDVYNDIKVSIDINPTSLM

Sequences:

>Translated_744_residues
MFNDKKGDKVYRYAGVVVNSNSIQLDRIFTYKISEELALKIKVGNRVLVPFGMGNKKIDAFVMNLYEDIENKENIKIKEI
IKIYEKFPVLSYKDIRIVEYMRKHCLSTYMEGIRTILPSILFKGVKKKKENLIYIGKSLEGRHNKEPYINIYNIVKENSG
KFNKTALSKKFDLSLSSINTLLKYEFLTQEEKVINRYNDKIYKGYEEKVLNDEQRSAIEKIMNSEKNMFLIKGVTGSGKT
EIYMNLVKEMLKEGKDSIILVPEIALTPQMIERFKGRFGKDITLFHSRLSDGEKYDEWVRVKKGEVKIAIGPRSAIFLPF
NNLGLIVIDEEHETSYKSDSDPKYNARDIGRIKSELYNCKLVLGTATPSIESYYASKIGELELIELFNRTDGAAMPKIEI
VDMREELISNNKSIFSKKLFEDIEERLNKKEQIILFLNRRGFSTFVSCRKCGYVFKCNNCDIALTYHNRNSFLICHYCGK
RERVLKTCPKCGSSYVKYFGAGTERIEKEVHKYFPKARTLRMDFDTTRKKNSHERIYETFKNGKADILIGTQMIAKGLDF
ENVTLVGVIAADLSLNIPDYKATERTFQLITQVSGRAGRGKKEGKVIVQTYSPDNYSIIYSANNEYDKFYKEEVEYRRLM
DYPPFTEIMLINLSSKNEDLLIKSIQNVGIILKDTLKNNDKINVLGPCPCEISKIKNNYRWQITLKGIISHELKHIIKNK
VYENLKDVYNDIKVSIDINPTSLM
>Mature_744_residues
MFNDKKGDKVYRYAGVVVNSNSIQLDRIFTYKISEELALKIKVGNRVLVPFGMGNKKIDAFVMNLYEDIENKENIKIKEI
IKIYEKFPVLSYKDIRIVEYMRKHCLSTYMEGIRTILPSILFKGVKKKKENLIYIGKSLEGRHNKEPYINIYNIVKENSG
KFNKTALSKKFDLSLSSINTLLKYEFLTQEEKVINRYNDKIYKGYEEKVLNDEQRSAIEKIMNSEKNMFLIKGVTGSGKT
EIYMNLVKEMLKEGKDSIILVPEIALTPQMIERFKGRFGKDITLFHSRLSDGEKYDEWVRVKKGEVKIAIGPRSAIFLPF
NNLGLIVIDEEHETSYKSDSDPKYNARDIGRIKSELYNCKLVLGTATPSIESYYASKIGELELIELFNRTDGAAMPKIEI
VDMREELISNNKSIFSKKLFEDIEERLNKKEQIILFLNRRGFSTFVSCRKCGYVFKCNNCDIALTYHNRNSFLICHYCGK
RERVLKTCPKCGSSYVKYFGAGTERIEKEVHKYFPKARTLRMDFDTTRKKNSHERIYETFKNGKADILIGTQMIAKGLDF
ENVTLVGVIAADLSLNIPDYKATERTFQLITQVSGRAGRGKKEGKVIVQTYSPDNYSIIYSANNEYDKFYKEEVEYRRLM
DYPPFTEIMLINLSSKNEDLLIKSIQNVGIILKDTLKNNDKINVLGPCPCEISKIKNNYRWQITLKGIISHELKHIIKNK
VYENLKDVYNDIKVSIDINPTSLM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=734, Percent_Identity=32.016348773842, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86178; Mature: 86178

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNDKKGDKVYRYAGVVVNSNSIQLDRIFTYKISEELALKIKVGNRVLVPFGMGNKKIDA
CCCCCCCCEEEEEEEEEEECCCEEEEEEEEEEECCEEEEEEEECCEEEEEECCCCCHHHH
FVMNLYEDIENKENIKIKEIIKIYEKFPVLSYKDIRIVEYMRKHCLSTYMEGIRTILPSI
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKGVKKKKENLIYIGKSLEGRHNKEPYINIYNIVKENSGKFNKTALSKKFDLSLSSINT
HHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCHHHCCCHHHHHH
LLKYEFLTQEEKVINRYNDKIYKGYEEKVLNDEQRSAIEKIMNSEKNMFLIKGVTGSGKT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EIYMNLVKEMLKEGKDSIILVPEIALTPQMIERFKGRFGKDITLFHSRLSDGEKYDEWVR
HHHHHHHHHHHHCCCCCEEEECCHHCCHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHE
VKKGEVKIAIGPRSAIFLPFNNLGLIVIDEEHETSYKSDSDPKYNARDIGRIKSELYNCK
EECCEEEEEECCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEE
LVLGTATPSIESYYASKIGELELIELFNRTDGAAMPKIEIVDMREELISNNKSIFSKKLF
EEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHH
EDIEERLNKKEQIILFLNRRGFSTFVSCRKCGYVFKCNNCDIALTYHNRNSFLICHYCGK
HHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEEECCCEEEEEEECCCCEEEEEECCC
RERVLKTCPKCGSSYVKYFGAGTERIEKEVHKYFPKARTLRMDFDTTRKKNSHERIYETF
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
KNGKADILIGTQMIAKGLDFENVTLVGVIAADLSLNIPDYKATERTFQLITQVSGRAGRG
HCCCEEEEEEHHHHHCCCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHHHHHCCCCCCC
KKEGKVIVQTYSPDNYSIIYSANNEYDKFYKEEVEYRRLMDYPPFTEIMLINLSSKNEDL
CCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHH
LIKSIQNVGIILKDTLKNNDKINVLGPCPCEISKIKNNYRWQITLKGIISHELKHIIKNK
HHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHH
VYENLKDVYNDIKVSIDINPTSLM
HHHHHHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure
MFNDKKGDKVYRYAGVVVNSNSIQLDRIFTYKISEELALKIKVGNRVLVPFGMGNKKIDA
CCCCCCCCEEEEEEEEEEECCCEEEEEEEEEEECCEEEEEEEECCEEEEEECCCCCHHHH
FVMNLYEDIENKENIKIKEIIKIYEKFPVLSYKDIRIVEYMRKHCLSTYMEGIRTILPSI
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKGVKKKKENLIYIGKSLEGRHNKEPYINIYNIVKENSGKFNKTALSKKFDLSLSSINT
HHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCHHHCCCHHHHHH
LLKYEFLTQEEKVINRYNDKIYKGYEEKVLNDEQRSAIEKIMNSEKNMFLIKGVTGSGKT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EIYMNLVKEMLKEGKDSIILVPEIALTPQMIERFKGRFGKDITLFHSRLSDGEKYDEWVR
HHHHHHHHHHHHCCCCCEEEECCHHCCHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHE
VKKGEVKIAIGPRSAIFLPFNNLGLIVIDEEHETSYKSDSDPKYNARDIGRIKSELYNCK
EECCEEEEEECCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEE
LVLGTATPSIESYYASKIGELELIELFNRTDGAAMPKIEIVDMREELISNNKSIFSKKLF
EEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHH
EDIEERLNKKEQIILFLNRRGFSTFVSCRKCGYVFKCNNCDIALTYHNRNSFLICHYCGK
HHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEEECCCEEEEEEECCCCEEEEEECCC
RERVLKTCPKCGSSYVKYFGAGTERIEKEVHKYFPKARTLRMDFDTTRKKNSHERIYETF
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
KNGKADILIGTQMIAKGLDFENVTLVGVIAADLSLNIPDYKATERTFQLITQVSGRAGRG
HCCCEEEEEEHHHHHCCCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHHHHHCCCCCCC
KKEGKVIVQTYSPDNYSIIYSANNEYDKFYKEEVEYRRLMDYPPFTEIMLINLSSKNEDL
CCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHH
LIKSIQNVGIILKDTLKNNDKINVLGPCPCEISKIKNNYRWQITLKGIISHELKHIIKNK
HHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHH
VYENLKDVYNDIKVSIDINPTSLM
HHHHHHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]