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The map label for this gene is lutR [H]

Identifier: 28210679

GI number: 28210679

Start: 1037992

End: 1038687

Strand: Direct

Name: lutR [H]

Synonym: CTC00971

Alternate gene names: 28210679

Gene position: 1037992-1038687 (Clockwise)

Preceding gene: 28210678

Following gene: 28210680

Centisome position: 37.08

GC content: 29.02

Gene sequence:

>696_bases
ATGTTTAGTCCTGTAAAGAATAAGAAAATATATGAAGAAGTTATAGAACAAATTAAAGATATGATATATAAAGGAATACT
AAAAAAAGGAGATAAACTTCCTTCAGAAAGAGATATGGTAGAGAAATTACAGGTTAGTAGAACATCTGTAAGAGAAGCTT
TAAGATCTTTAGAAATTGTTGGTCTTATTGAAAGTAGGCAAGGAGAAGGAAATTTCATAAAAGAGGATTTTGAGGATGGA
CTTATAGAACCGCTTTCAGTAATGTTTATGCTTCAGGATAGCAAATCAGAAGAAATTTTAGAGCTAAGAAAGATTATAGA
AGTAGGAACAATTAAGCTAGCTGCAGAACGGATAACAGAAAGTGAGTTAGAGAAGTTAAATGAGCTTAAAGAAAAGTTAA
AAACTCCTAATAAAGAAGAAAATTTAGCAGAAATAGATAGAGAATTTCACTATAAAATAGCAGCTGCTTCTAAAAACTTT
TTATTATTAGGAGTATTAAATGCAATTTCAGCGCTAATGGAGTCTTACATAAAAGATGCTAGAAAAAGTATTTTAGAAGA
AGAAGGAAATAGACAAGAACTTATAACACAGCATGAAAATATTTTTGAAGCATTAAATAATAATAGTGAGGATGAAGCTG
TAGAAGCTATGACAAAGCATTTGGACTTTGCAAATAAATACATGATAAATAGATAA

Upstream 100 bases:

>100_bases
TTATTACTAATTGGTTTAGAGTAATAAAGGAAAAATAGAGGCTAAGAAACCTTTATTTTTCTTTGGAAAATAATATATTA
ACATAGAAAGGAAGAAAAAA

Downstream 100 bases:

>100_bases
TATTAATTAGAAGATGTCTTTTAAAAAAGACATCTTTTTTATTTTAAAATATATAGCAATGTTCTAAAAATTGTGATAAT
ATATATTTAACTGGTACTAT

Product: transcriptional regulator, putative pyruvate dehydrogenase complex repressor

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFSPVKNKKIYEEVIEQIKDMIYKGILKKGDKLPSERDMVEKLQVSRTSVREALRSLEIVGLIESRQGEGNFIKEDFEDG
LIEPLSVMFMLQDSKSEEILELRKIIEVGTIKLAAERITESELEKLNELKEKLKTPNKEENLAEIDREFHYKIAAASKNF
LLLGVLNAISALMESYIKDARKSILEEEGNRQELITQHENIFEALNNNSEDEAVEAMTKHLDFANKYMINR

Sequences:

>Translated_231_residues
MFSPVKNKKIYEEVIEQIKDMIYKGILKKGDKLPSERDMVEKLQVSRTSVREALRSLEIVGLIESRQGEGNFIKEDFEDG
LIEPLSVMFMLQDSKSEEILELRKIIEVGTIKLAAERITESELEKLNELKEKLKTPNKEENLAEIDREFHYKIAAASKNF
LLLGVLNAISALMESYIKDARKSILEEEGNRQELITQHENIFEALNNNSEDEAVEAMTKHLDFANKYMINR
>Mature_231_residues
MFSPVKNKKIYEEVIEQIKDMIYKGILKKGDKLPSERDMVEKLQVSRTSVREALRSLEIVGLIESRQGEGNFIKEDFEDG
LIEPLSVMFMLQDSKSEEILELRKIIEVGTIKLAAERITESELEKLNELKEKLKTPNKEENLAEIDREFHYKIAAASKNF
LLLGVLNAISALMESYIKDARKSILEEEGNRQELITQHENIFEALNNNSEDEAVEAMTKHLDFANKYMINR

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=229, Percent_Identity=31.8777292576419, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786303, Length=239, Percent_Identity=28.0334728033473, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789353, Length=220, Percent_Identity=29.5454545454545, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790780, Length=235, Percent_Identity=29.3617021276596, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1789621, Length=226, Percent_Identity=28.3185840707965, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082208, Length=223, Percent_Identity=26.457399103139, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994955, Length=200, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26665; Mature: 26665

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSPVKNKKIYEEVIEQIKDMIYKGILKKGDKLPSERDMVEKLQVSRTSVREALRSLEIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLIESRQGEGNFIKEDFEDGLIEPLSVMFMLQDSKSEEILELRKIIEVGTIKLAAERITE
HHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SELEKLNELKEKLKTPNKEENLAEIDREFHYKIAAASKNFLLLGVLNAISALMESYIKDA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RKSILEEEGNRQELITQHENIFEALNNNSEDEAVEAMTKHLDFANKYMINR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSPVKNKKIYEEVIEQIKDMIYKGILKKGDKLPSERDMVEKLQVSRTSVREALRSLEIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLIESRQGEGNFIKEDFEDGLIEPLSVMFMLQDSKSEEILELRKIIEVGTIKLAAERITE
HHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SELEKLNELKEKLKTPNKEENLAEIDREFHYKIAAASKNFLLLGVLNAISALMESYIKDA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RKSILEEEGNRQELITQHENIFEALNNNSEDEAVEAMTKHLDFANKYMINR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]