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The map label for this gene is kpsF [C]

Identifier: 28210619

GI number: 28210619

Start: 962555

End: 963166

Strand: Direct

Name: kpsF [C]

Synonym: CTC00908

Alternate gene names: 28210619

Gene position: 962555-963166 (Clockwise)

Preceding gene: 28210618

Following gene: 28210620

Centisome position: 34.39

GC content: 32.52

Gene sequence:

>612_bases
TTGATGAATAAAGATGTATTATTTTCTATAAAAGAAGTAATGGAAGAAGAAATAAAAGCTATAAAAAACGTTTGTGAAAA
TCTAGACGAGAATTACGAGAAAGCAGTAGATTTAATTCATAACTGTAAAGGAAAAGTTGTTTTTACTGGAGTAGGTAAAT
CAGGACATATAGGAGAAAAGCTTGCAGCAACATTTGCAAGTACTGGAACACCAGCTTTCTTTGTGCACTCAACAGAAGCT
CTTCATGGAGATTTAGGAATGATAGAAGAAAAAGATATAGTTATTGCTATATCAAATAGTGGAGAAACTAAAGAAGTACT
TTCAATAATTTCATCTATAAAGTACATAGGAAGTAAGATAATATCAATTACAGGAAATAACAATTCTTCCCTTGCAAAAG
AAAGTGATGTAGCACTTGAAGCAAAGGTTGATCATGAAGCGGACCCATTAAATCTTGCACCAACTAGTAGTTCTACTGTA
GCTTTAGTTTTAGGAGATGCACTTGCCATAACTCTTTCTCAACTTAAAGAATTTAAGAGGGAAAATTTTGCAGTATTTCA
TCCCGGCGGAAGTCTAGGTAAGAGATTGTTTAATGAGATGAAAAAGCTTTAA

Upstream 100 bases:

>100_bases
TATAAACGATCCAGAAGTACAAAAAGTTTTATATCCAGAAAAAAATAAATAATAAGTATTCTGATATTTAAGTTTTTATA
TAGAAGAATGGGAGAGATAG

Downstream 100 bases:

>100_bases
TAAAAGGAGAATGAATGTGAAGAAGATACTAGTAATAGGTAGTTTTATGATGGATCTAGTGGCACAAACTCCAAGAGCAC
CAATAGAGGGTGAAACAATT

Product: polysialic acid capsule expression protein kpsF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MMNKDVLFSIKEVMEEEIKAIKNVCENLDENYEKAVDLIHNCKGKVVFTGVGKSGHIGEKLAATFASTGTPAFFVHSTEA
LHGDLGMIEEKDIVIAISNSGETKEVLSIISSIKYIGSKIISITGNNNSSLAKESDVALEAKVDHEADPLNLAPTSSSTV
ALVLGDALAITLSQLKEFKRENFAVFHPGGSLGKRLFNEMKKL

Sequences:

>Translated_203_residues
MMNKDVLFSIKEVMEEEIKAIKNVCENLDENYEKAVDLIHNCKGKVVFTGVGKSGHIGEKLAATFASTGTPAFFVHSTEA
LHGDLGMIEEKDIVIAISNSGETKEVLSIISSIKYIGSKIISITGNNNSSLAKESDVALEAKVDHEADPLNLAPTSSSTV
ALVLGDALAITLSQLKEFKRENFAVFHPGGSLGKRLFNEMKKL
>Mature_203_residues
MMNKDVLFSIKEVMEEEIKAIKNVCENLDENYEKAVDLIHNCKGKVVFTGVGKSGHIGEKLAATFASTGTPAFFVHSTEA
LHGDLGMIEEKDIVIAISNSGETKEVLSIISSIKYIGSKIISITGNNNSSLAKESDVALEAKVDHEADPLNLAPTSSSTV
ALVLGDALAITLSQLKEFKRENFAVFHPGGSLGKRLFNEMKKL

Specific function: Unknown

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=187, Percent_Identity=52.4064171122995, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI87082151, Length=202, Percent_Identity=44.5544554455446, Blast_Score=184, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 21895; Mature: 21895

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNKDVLFSIKEVMEEEIKAIKNVCENLDENYEKAVDLIHNCKGKVVFTGVGKSGHIGEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
LAATFASTGTPAFFVHSTEALHGDLGMIEEKDIVIAISNSGETKEVLSIISSIKYIGSKI
HHHHHHCCCCCEEEEECCHHHCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISITGNNNSSLAKESDVALEAKVDHEADPLNLAPTSSSTVALVLGDALAITLSQLKEFKR
EEEECCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
ENFAVFHPGGSLGKRLFNEMKKL
CCEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMNKDVLFSIKEVMEEEIKAIKNVCENLDENYEKAVDLIHNCKGKVVFTGVGKSGHIGEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
LAATFASTGTPAFFVHSTEALHGDLGMIEEKDIVIAISNSGETKEVLSIISSIKYIGSKI
HHHHHHCCCCCEEEEECCHHHCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISITGNNNSSLAKESDVALEAKVDHEADPLNLAPTSSSTVALVLGDALAITLSQLKEFKR
EEEECCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
ENFAVFHPGGSLGKRLFNEMKKL
CCEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]