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The map label for this gene is rbsB [H]

Identifier: 28210618

GI number: 28210618

Start: 961532

End: 962506

Strand: Direct

Name: rbsB [H]

Synonym: CTC00907

Alternate gene names: 28210618

Gene position: 961532-962506 (Clockwise)

Preceding gene: 28210617

Following gene: 28210619

Centisome position: 34.35

GC content: 34.67

Gene sequence:

>975_bases
ATGTTAAAGAAAAGATTAGCTTTAATAACAATGTCAGTAATGTTGTCAGCAGGAGTTTTAGCGGGATGCGGACAAAAATC
AGCAAGTGATTCTACAAAGAAAAATATTGCACTAATAGTTAAGTCAACAGATTCTGAATACTGGCTTAGCGTAAAGGCAG
GAGCAGAGAAAGCTGCTAAAGAAGCAGGGGCAAATATAATATTTAAAGGACCTGCTACAGAACAAGATATTCAAGGAGAA
GTAAATATAGTTGAAGATGCTATAAATCAAAAAGTTGATGGAATAGTACTTGCAGCTAGCGATACAAAGGCTCTTATTCA
ACCAGTTGAGAATGCTATAAATGCAAAAATACCTTTAGTAACTGTTGACTCAGGAGTGGATTCTGATAAGGTAGCAAGTT
TTATAGCAACAGATAACGAAAAGGCAGCAAGTCAAGCAGCTGATGTTTGTGCAGAACTTATTGGAAAATCAGGTAAGGTT
GGAGTAGTAAACTTTGTACCAGGTGTTTCAACAGCAGTTCAAAGAGAAAAAGGATTTAGAGATGGAATGAGTAAACATAG
CGGAATAGAGTTACTTAAAACACAATATTCACAAAGTGACAAATCAAAAGCCATGGCTATAACAGAAGATATTCTTACTG
CCAATCCAGATGTAAAAGCAATTTTTGCAGCTAATAACCGTTCAGCTTTAGGTGTAGCACAAGCACTTAAAGGAAAGGGA
GTTGCTGGTAAAGTAAAAGTTGTAGCTTTTGATGCTGATCCAGATGAAATTAAAGGCATTGAAGATGGTTCAATAACAGC
TCTTATAGTACAAAATCCTTATAAAATGGGAGAAGAAGGGGTTAAAAATGTTCTTGCTCTTATGAAGGGTGAAACAGTAG
AAAAAAGAATAGATACAGGTGTTACGATAGTTACAAAAGAAAATATAAACGATCCAGAAGTACAAAAAGTTTTATATCCA
GAAAAAAATAAATAA

Upstream 100 bases:

>100_bases
AGCTGCTGTATATGGAGATAAGTTGAAAAATAAAAAATTAAATTAGTTTATGTTATTTAAATATAATATAAAAATAAAAT
AATTTTAGGGGGAATATCAA

Downstream 100 bases:

>100_bases
TAAGTATTCTGATATTTAAGTTTTTATATAGAAGAATGGGAGAGATAGTTGATGAATAAAGATGTATTATTTTCTATAAA
AGAAGTAATGGAAGAAGAAA

Product: D-ribose-binding periplasmic protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLKKRLALITMSVMLSAGVLAGCGQKSASDSTKKNIALIVKSTDSEYWLSVKAGAEKAAKEAGANIIFKGPATEQDIQGE
VNIVEDAINQKVDGIVLAASDTKALIQPVENAINAKIPLVTVDSGVDSDKVASFIATDNEKAASQAADVCAELIGKSGKV
GVVNFVPGVSTAVQREKGFRDGMSKHSGIELLKTQYSQSDKSKAMAITEDILTANPDVKAIFAANNRSALGVAQALKGKG
VAGKVKVVAFDADPDEIKGIEDGSITALIVQNPYKMGEEGVKNVLALMKGETVEKRIDTGVTIVTKENINDPEVQKVLYP
EKNK

Sequences:

>Translated_324_residues
MLKKRLALITMSVMLSAGVLAGCGQKSASDSTKKNIALIVKSTDSEYWLSVKAGAEKAAKEAGANIIFKGPATEQDIQGE
VNIVEDAINQKVDGIVLAASDTKALIQPVENAINAKIPLVTVDSGVDSDKVASFIATDNEKAASQAADVCAELIGKSGKV
GVVNFVPGVSTAVQREKGFRDGMSKHSGIELLKTQYSQSDKSKAMAITEDILTANPDVKAIFAANNRSALGVAQALKGKG
VAGKVKVVAFDADPDEIKGIEDGSITALIVQNPYKMGEEGVKNVLALMKGETVEKRIDTGVTIVTKENINDPEVQKVLYP
EKNK
>Mature_324_residues
MLKKRLALITMSVMLSAGVLAGCGQKSASDSTKKNIALIVKSTDSEYWLSVKAGAEKAAKEAGANIIFKGPATEQDIQGE
VNIVEDAINQKVDGIVLAASDTKALIQPVENAINAKIPLVTVDSGVDSDKVASFIATDNEKAASQAADVCAELIGKSGKV
GVVNFVPGVSTAVQREKGFRDGMSKHSGIELLKTQYSQSDKSKAMAITEDILTANPDVKAIFAANNRSALGVAQALKGKG
VAGKVKVVAFDADPDEIKGIEDGSITALIVQNPYKMGEEGVKNVLALMKGETVEKRIDTGVTIVTKENINDPEVQKVLYP
EKNK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=309, Percent_Identity=32.3624595469256, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790526, Length=275, Percent_Identity=32.3636363636364, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787795, Length=198, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788898, Length=272, Percent_Identity=23.8970588235294, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34085; Mature: 34085

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKRLALITMSVMLSAGVLAGCGQKSASDSTKKNIALIVKSTDSEYWLSVKAGAEKAAK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCHHHHHH
EAGANIIFKGPATEQDIQGEVNIVEDAINQKVDGIVLAASDTKALIQPVENAINAKIPLV
HCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEE
TVDSGVDSDKVASFIATDNEKAASQAADVCAELIGKSGKVGVVNFVPGVSTAVQREKGFR
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
DGMSKHSGIELLKTQYSQSDKSKAMAITEDILTANPDVKAIFAANNRSALGVAQALKGKG
HCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCC
VAGKVKVVAFDADPDEIKGIEDGSITALIVQNPYKMGEEGVKNVLALMKGETVEKRIDTG
CCCEEEEEEECCCHHHHCCCCCCCEEEEEEECCHHCCHHHHHHHHHHHCCCHHHHHHCCC
VTIVTKENINDPEVQKVLYPEKNK
EEEEEECCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MLKKRLALITMSVMLSAGVLAGCGQKSASDSTKKNIALIVKSTDSEYWLSVKAGAEKAAK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCHHHHHH
EAGANIIFKGPATEQDIQGEVNIVEDAINQKVDGIVLAASDTKALIQPVENAINAKIPLV
HCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEE
TVDSGVDSDKVASFIATDNEKAASQAADVCAELIGKSGKVGVVNFVPGVSTAVQREKGFR
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
DGMSKHSGIELLKTQYSQSDKSKAMAITEDILTANPDVKAIFAANNRSALGVAQALKGKG
HCCCHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCC
VAGKVKVVAFDADPDEIKGIEDGSITALIVQNPYKMGEEGVKNVLALMKGETVEKRIDTG
CCCEEEEEEECCCHHHHCCCCCCCEEEEEEECCHHCCHHHHHHHHHHHCCCHHHHHHCCC
VTIVTKENINDPEVQKVLYPEKNK
EEEEEECCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]