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The map label for this gene is lacI [H]

Identifier: 28210579

GI number: 28210579

Start: 916580

End: 917584

Strand: Direct

Name: lacI [H]

Synonym: CTC00863

Alternate gene names: 28210579

Gene position: 916580-917584 (Clockwise)

Preceding gene: 28210578

Following gene: 28210580

Centisome position: 32.74

GC content: 27.26

Gene sequence:

>1005_bases
ATGATGCCAACTATTAATGACGTAGCAAGAGAAGCAGGAGTTTCTATAAGTACAGTATCAAGAGTCTTAAACAATAATTA
CCCTGTAAAAGAAGAAACGAGAAAGAGAATAGAGGAAGCTATAGAGAAGCTAAATTACAAGCCTAATATGTTAGCAAGAA
GTCTTATAACTAAAAAAACTTCTACTATAGGGGTAATTGTTCCGGGAATAACTAATTTATTTTTTCCTACTATAGTAGAG
AGTATAGAAGATTATAGTAAAGATCAAGGATATAGTATTGTATTATGTAATACTAGAGGAGAAGCTTTAAAAGAAAAAGA
AATTATAGAAAATTTAATGTCAAAGGGTGTAGATGGAATAATTATTATAGACCCTACCTTAGATAATTTGAAAAACAAAT
ATTTAGAAGATATATCAATGAAATTACCCATTATAATAGTAAATGGATCACCAGAAGAACTTAAAGGTAATTTTATATGT
TATGATGAAAAAGTTGGAACATTAGAAGCTTTTGAATATTTGTTGGAATTAGGTCATAAGGAAATAGCATTTATAAGAGG
CTCTAAGAGCTATTCCTATGATATTAAAGAGAAAATTTATACAGATATTATAGAAAAAAATAATTTAAAGTATAATGAAA
TATTAGACGTAGGTAAAGGTAATAGTATAGAAGTTGTAGAAAATACTCAAAAAAAGGTTGAAGAATTTCTTTTAAATAAA
AATAAACCTACGGCCATATTTGCATGTAATGATTTAATGGCATTAGGTGTTGTAAATGCATGCAATAAGTTAAACTTAAA
TGTACCACAGGATATTTCCATAATTGGATTTGACAATACATTAATATCTAATATAATCCATCCTAAACTTACCACAGTGG
ATCTTAATATGAAAAGAATAGGTAACATAGCTGCTTTAAAATTACTAGAGATTATAGAAGATAAAAATAAATCCATAAAG
TATACTATGGAGACCAAACTTATAGTTAGGGAAAGCTGCTATTAA

Upstream 100 bases:

>100_bases
ATAATATTTTCAATAATTTAATAGAATTTGCCATAATGAAGTGATAGAATAAATTAAAGGAATAATTGTAACTAACTAAA
GATGCCAAAAGGATGATTTA

Downstream 100 bases:

>100_bases
AATGAGTTTAATTAAAAAGTGTGTGCTTATGCACTTTTTAATTAAATTTGTAGAAAAGGTTTTCTGTAAGGAGGTAATTA
TTTATGATTGATATAAAAAG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Catabolite control protein [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MMPTINDVAREAGVSISTVSRVLNNNYPVKEETRKRIEEAIEKLNYKPNMLARSLITKKTSTIGVIVPGITNLFFPTIVE
SIEDYSKDQGYSIVLCNTRGEALKEKEIIENLMSKGVDGIIIIDPTLDNLKNKYLEDISMKLPIIIVNGSPEELKGNFIC
YDEKVGTLEAFEYLLELGHKEIAFIRGSKSYSYDIKEKIYTDIIEKNNLKYNEILDVGKGNSIEVVENTQKKVEEFLLNK
NKPTAIFACNDLMALGVVNACNKLNLNVPQDISIIGFDNTLISNIIHPKLTTVDLNMKRIGNIAALKLLEIIEDKNKSIK
YTMETKLIVRESCY

Sequences:

>Translated_334_residues
MMPTINDVAREAGVSISTVSRVLNNNYPVKEETRKRIEEAIEKLNYKPNMLARSLITKKTSTIGVIVPGITNLFFPTIVE
SIEDYSKDQGYSIVLCNTRGEALKEKEIIENLMSKGVDGIIIIDPTLDNLKNKYLEDISMKLPIIIVNGSPEELKGNFIC
YDEKVGTLEAFEYLLELGHKEIAFIRGSKSYSYDIKEKIYTDIIEKNNLKYNEILDVGKGNSIEVVENTQKKVEEFLLNK
NKPTAIFACNDLMALGVVNACNKLNLNVPQDISIIGFDNTLISNIIHPKLTTVDLNMKRIGNIAALKLLEIIEDKNKSIK
YTMETKLIVRESCY
>Mature_334_residues
MMPTINDVAREAGVSISTVSRVLNNNYPVKEETRKRIEEAIEKLNYKPNMLARSLITKKTSTIGVIVPGITNLFFPTIVE
SIEDYSKDQGYSIVLCNTRGEALKEKEIIENLMSKGVDGIIIIDPTLDNLKNKYLEDISMKLPIIIVNGSPEELKGNFIC
YDEKVGTLEAFEYLLELGHKEIAFIRGSKSYSYDIKEKIYTDIIEKNNLKYNEILDVGKGNSIEVVENTQKKVEEFLLNK
NKPTAIFACNDLMALGVVNACNKLNLNVPQDISIIGFDNTLISNIIHPKLTTVDLNMKRIGNIAALKLLEIIEDKNKSIK
YTMETKLIVRESCY

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (amyE) and the acetyl-coenzyme A synthetase (acsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=31.9078947368421, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=30.2114803625378, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=29.7619047619048, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=30.1775147928994, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=30.5084745762712, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=31.3915857605178, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=30.4075235109718, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=27.1341463414634, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=25.0737463126844, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=26.3492063492064, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786268, Length=327, Percent_Identity=23.5474006116208, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=23.2704402515723, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787906, Length=266, Percent_Identity=27.4436090225564, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37678; Mature: 37678

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPTINDVAREAGVSISTVSRVLNNNYPVKEETRKRIEEAIEKLNYKPNMLARSLITKKT
CCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
STIGVIVPGITNLFFPTIVESIEDYSKDQGYSIVLCNTRGEALKEKEIIENLMSKGVDGI
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
IIIDPTLDNLKNKYLEDISMKLPIIIVNGSPEELKGNFICYDEKVGTLEAFEYLLELGHK
EEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCEEEEECCCCHHHHHHHHHHCCCC
EIAFIRGSKSYSYDIKEKIYTDIIEKNNLKYNEILDVGKGNSIEVVENTQKKVEEFLLNK
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHCC
NKPTAIFACNDLMALGVVNACNKLNLNVPQDISIIGFDNTLISNIIHPKLTTVDLNMKRI
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEEECCHHHH
GNIAALKLLEIIEDKNKSIKYTMETKLIVRESCY
CCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCC
>Mature Secondary Structure
MMPTINDVAREAGVSISTVSRVLNNNYPVKEETRKRIEEAIEKLNYKPNMLARSLITKKT
CCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
STIGVIVPGITNLFFPTIVESIEDYSKDQGYSIVLCNTRGEALKEKEIIENLMSKGVDGI
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
IIIDPTLDNLKNKYLEDISMKLPIIIVNGSPEELKGNFICYDEKVGTLEAFEYLLELGHK
EEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCEEEEECCCCHHHHHHHHHHCCCC
EIAFIRGSKSYSYDIKEKIYTDIIEKNNLKYNEILDVGKGNSIEVVENTQKKVEEFLLNK
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHCC
NKPTAIFACNDLMALGVVNACNKLNLNVPQDISIIGFDNTLISNIIHPKLTTVDLNMKRI
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEEECCHHHH
GNIAALKLLEIIEDKNKSIKYTMETKLIVRESCY
CCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8200532 [H]