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The map label for this gene is aspA [H]

Identifier: 28210541

GI number: 28210541

Start: 872757

End: 874169

Strand: Direct

Name: aspA [H]

Synonym: CTC00824

Alternate gene names: 28210541

Gene position: 872757-874169 (Clockwise)

Preceding gene: 28210540

Following gene: 28210542

Centisome position: 31.18

GC content: 31.56

Gene sequence:

>1413_bases
ATGCAATACAGATTAGAAAGTGATTCAGTAGGTGAAAAAAGAATACCAAAGGATGCTTATTATGGTGTGCAAAGTCTTAG
AGCACAAGAGAATTTTATGATAACAGGAAAAAAAGTCCACACGGAAATGATAAAAGCATTAGCAGAGGTAAAAAAAGCAA
CAGCTATGACAAATATGCAAGTGGAACTTTTAAGTAAAAAAGCAGGTAACGCCATTGTAAAAGCTTGTGATGAAATAATT
GAAGGAAATTTACAAGAACAGTTTATAACTGATCCTATACAAGGTGGCGCAGGAACATCTATGAACATGAATTTAAACGA
AGTAATTGCTAATAGAGCAGTAGAAATATTAGGTGGGGAAAAGGGAGATTATAGTATAGTTCATCCTAATGATCATGTAA
ATATGGGACAATCTACTAACGATGTTATTCCTACAGGGGGAAAAATAGCAACTATAAGACTTTTAGATAGAGCATTAATG
TCATTAGAAAATTTAGTAGATGCACTTACAAATAAATCTAAGGAATTTGATCATATTATAAAAATGGGGAGAACTCAAAT
GCAAGATGCTGTACCAATAAGATTAGGACAAGAATTTAAGGCATATTCAAATGCTATAAAAAGGGACATAGTTAGAATTA
AGAGAGCAAAAGAAGAATTATATCATGTAAGTATTGGGGCTACAGCTATAGGTACAGGTATAAATGCTGATGAGGAGTAT
GTAAAAAATATAACACCAAATTTATCAAAAGTAACAACACTTAGCCTAATTCAATGTGAAGATTTAGTAGATGGAACTCA
AAATCTAGATTGTTTTGCTTCAGTATCATCAAATTTAAAGGTTTGTGCTTTAAGTTTATCTAAAATAGCTAATGATTTAA
GACTTATGTCCTCAGGACCTAGAACCGGTTTTGGAGAAATAAATCTTCCAGCAAAACAAAATGGGTCATCTATAATGCCA
GGAAAAGTAAATCCAGTAATAGCTGAAGTTGTAAACCAAGTTGCATTTAGTATTGTAGGAAATGATGTAACTATTAGCAT
GGCTGTTGAAGCAGGACAATTAGAATTAAACGCATTTGAGCCGGTGTTATATTATAAGTTATTTGAATCTATTGAAATAT
TGAAAAATGGTGTGGATACATTTATTCATAATTGTATAAATGGTATAACAGCAAATGAGGAAAGATGTAAAAAGTTAGTG
GATAATAGTGTTGGAATAATTACTGCAATATGTCCACATGTAGGATATAAAAAAGCAGCTAAAGTAGCAAAAACTGCTAT
ACAGACTGGAGAATCTGTAAAGGAGCTTGTATTAAAAGAAAATTTATTATCAGAAAAAGAATTGGATACAATACTTGACC
CATTTGGAATGACTAAACCAGGAATATCTGGGAAAGAGCTGTTAAAAAAGTAA

Upstream 100 bases:

>100_bases
TTCCACAGATATTTTAGCCAAGGAAACAATAAAAAGGGTTAAAAAATAGCTATTTATTCAAACTTTATTTATTTAAAATA
GTATAAGGGAGGAATTTTAT

Downstream 100 bases:

>100_bases
AAAGGTTATAAAAAGTCTGCAAAAATGCAGACTTTTTATTTACTAGATGGTAAAATTTAATTAGGATGAATATAAGAAGG
AGGAAGAATCATGTATAATT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQYRLESDSVGEKRIPKDAYYGVQSLRAQENFMITGKKVHTEMIKALAEVKKATAMTNMQVELLSKKAGNAIVKACDEII
EGNLQEQFITDPIQGGAGTSMNMNLNEVIANRAVEILGGEKGDYSIVHPNDHVNMGQSTNDVIPTGGKIATIRLLDRALM
SLENLVDALTNKSKEFDHIIKMGRTQMQDAVPIRLGQEFKAYSNAIKRDIVRIKRAKEELYHVSIGATAIGTGINADEEY
VKNITPNLSKVTTLSLIQCEDLVDGTQNLDCFASVSSNLKVCALSLSKIANDLRLMSSGPRTGFGEINLPAKQNGSSIMP
GKVNPVIAEVVNQVAFSIVGNDVTISMAVEAGQLELNAFEPVLYYKLFESIEILKNGVDTFIHNCINGITANEERCKKLV
DNSVGIITAICPHVGYKKAAKVAKTAIQTGESVKELVLKENLLSEKELDTILDPFGMTKPGISGKELLKK

Sequences:

>Translated_470_residues
MQYRLESDSVGEKRIPKDAYYGVQSLRAQENFMITGKKVHTEMIKALAEVKKATAMTNMQVELLSKKAGNAIVKACDEII
EGNLQEQFITDPIQGGAGTSMNMNLNEVIANRAVEILGGEKGDYSIVHPNDHVNMGQSTNDVIPTGGKIATIRLLDRALM
SLENLVDALTNKSKEFDHIIKMGRTQMQDAVPIRLGQEFKAYSNAIKRDIVRIKRAKEELYHVSIGATAIGTGINADEEY
VKNITPNLSKVTTLSLIQCEDLVDGTQNLDCFASVSSNLKVCALSLSKIANDLRLMSSGPRTGFGEINLPAKQNGSSIMP
GKVNPVIAEVVNQVAFSIVGNDVTISMAVEAGQLELNAFEPVLYYKLFESIEILKNGVDTFIHNCINGITANEERCKKLV
DNSVGIITAICPHVGYKKAAKVAKTAIQTGESVKELVLKENLLSEKELDTILDPFGMTKPGISGKELLKK
>Mature_470_residues
MQYRLESDSVGEKRIPKDAYYGVQSLRAQENFMITGKKVHTEMIKALAEVKKATAMTNMQVELLSKKAGNAIVKACDEII
EGNLQEQFITDPIQGGAGTSMNMNLNEVIANRAVEILGGEKGDYSIVHPNDHVNMGQSTNDVIPTGGKIATIRLLDRALM
SLENLVDALTNKSKEFDHIIKMGRTQMQDAVPIRLGQEFKAYSNAIKRDIVRIKRAKEELYHVSIGATAIGTGINADEEY
VKNITPNLSKVTTLSLIQCEDLVDGTQNLDCFASVSSNLKVCALSLSKIANDLRLMSSGPRTGFGEINLPAKQNGSSIMP
GKVNPVIAEVVNQVAFSIVGNDVTISMAVEAGQLELNAFEPVLYYKLFESIEILKNGVDTFIHNCINGITANEERCKKLV
DNSVGIITAICPHVGYKKAAKVAKTAIQTGESVKELVLKENLLSEKELDTILDPFGMTKPGISGKELLKK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=42.7331887201735, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=47.9569892473118, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=38.6609071274298, Blast_Score=347, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=41.304347826087, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=38.2262996941896, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=40.5639913232104, Blast_Score=349, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=40.6047516198704, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=40.6047516198704, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.7408207343413, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=38.7096774193548, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=37.4458874458874, Blast_Score=331, Evalue=8e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51215; Mature: 51215

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYRLESDSVGEKRIPKDAYYGVQSLRAQENFMITGKKVHTEMIKALAEVKKATAMTNMQ
CCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VELLSKKAGNAIVKACDEIIEGNLQEQFITDPIQGGAGTSMNMNLNEVIANRAVEILGGE
HHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYSIVHPNDHVNMGQSTNDVIPTGGKIATIRLLDRALMSLENLVDALTNKSKEFDHII
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KMGRTQMQDAVPIRLGQEFKAYSNAIKRDIVRIKRAKEELYHVSIGATAIGTGINADEEY
HHCHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCCCHHH
VKNITPNLSKVTTLSLIQCEDLVDGTQNLDCFASVSSNLKVCALSLSKIANDLRLMSSGP
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
RTGFGEINLPAKQNGSSIMPGKVNPVIAEVVNQVAFSIVGNDVTISMAVEAGQLELNAFE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEECCCC
PVLYYKLFESIEILKNGVDTFIHNCINGITANEERCKKLVDNSVGIITAICPHVGYKKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHH
KVAKTAIQTGESVKELVLKENLLSEKELDTILDPFGMTKPGISGKELLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MQYRLESDSVGEKRIPKDAYYGVQSLRAQENFMITGKKVHTEMIKALAEVKKATAMTNMQ
CCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VELLSKKAGNAIVKACDEIIEGNLQEQFITDPIQGGAGTSMNMNLNEVIANRAVEILGGE
HHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYSIVHPNDHVNMGQSTNDVIPTGGKIATIRLLDRALMSLENLVDALTNKSKEFDHII
CCCEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KMGRTQMQDAVPIRLGQEFKAYSNAIKRDIVRIKRAKEELYHVSIGATAIGTGINADEEY
HHCHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCCCHHH
VKNITPNLSKVTTLSLIQCEDLVDGTQNLDCFASVSSNLKVCALSLSKIANDLRLMSSGP
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
RTGFGEINLPAKQNGSSIMPGKVNPVIAEVVNQVAFSIVGNDVTISMAVEAGQLELNAFE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEECCCC
PVLYYKLFESIEILKNGVDTFIHNCINGITANEERCKKLVDNSVGIITAICPHVGYKKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHH
KVAKTAIQTGESVKELVLKENLLSEKELDTILDPFGMTKPGISGKELLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]