Click here to switch to the map view.

The map label for this gene is comEC [H]

Identifier: 28210538

GI number: 28210538

Start: 869578

End: 870492

Strand: Direct

Name: comEC [H]

Synonym: CTC00821

Alternate gene names: 28210538

Gene position: 869578-870492 (Clockwise)

Preceding gene: 28210537

Following gene: 28210539

Centisome position: 31.06

GC content: 26.99

Gene sequence:

>915_bases
ATGAAAAAAATAAGAAATAGTATAAAAAAGATAAGTTTTATATTTATAATAGTAGCTATGATTTTTTCAATGGTTGCTTG
TTCAGAAATAGGTGAACAAAGTTCTACAAGTAAATCAAATAATGGAATTTTAAAAGTTCATTACATTAATGTTGGACAAG
GAGACAGTATATTAGTACAGCAAGACAATGAAAATATGTTAATAGATGCAGGTCCCAACTCTTCATCAGAAAAATTAGTA
AATTATTTAAAACAAAATGGTATTAAAGAAATAAAATATTTAGTAGCCACACATCCTCATGAAGATCATATAGGAGGAAT
GAACAAAGTAATAAATAATTTTAAAGTGGAAAGTGTTTATATGCCTAAAAAAATTGCTAATACAAAAACTTTTAAGAATA
TGGTAGAAGCTATGAAGAAAAACAATTTAAAAGCTAAAACACCTCGTGTTGGCGATAAAATTAATTTAGGTGATGCTAAG
TTTACTATATTATGGCCAAATGAACAAGAGCAAAGCAATACAAACAACTATTCTATAGTTTTAAAAGGGCAATATGGAGA
AAATACTTTCTTATTTACTGGTGATGCGGAACAATTAGCTGAAAAAAGTATATTGAGAAATAATACGGATATTAGAGCAG
AAGTTTTAAAGTTAGGACATCATGGAAGTAGTACATCTACATCAGACAAATTTTTAGAAAAAGTAAACCCTAAATATGCA
GTTGCTAGTTGTGAAAAGGGTAATAGTTATGGACATCCACATAGAGAAATTGTGAAGAAATTAAAAGATAAAAATATAAA
ACTTTATAGAACAGATGAAAATGGAGACATTGTATTAATAAGTGATGGTAAGAATATAAAATTTAATGTAGAACCTGGAA
GTTATAATTCAGGAAGTAGTAGTAAAAAGAAATGA

Upstream 100 bases:

>100_bases
ATTTACCGAAGAACATTATAAAGGTGCTGAAAAGATTTTTAAGAAAATGTTTGAAGAGAAGAAAGAAAAACTGAAAGAGG
GCAATTGAGGTGATGCATTT

Downstream 100 bases:

>100_bases
AGGGATTTTCCTTTCGTTTCTTTTTTATTTAAGAAAAACTCATAAAATAATATAGTGGGAATATCTTTATAATAATGAAC
ATCTAACAATGAATAATTTC

Product: ComE-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKKIRNSIKKISFIFIIVAMIFSMVACSEIGEQSSTSKSNNGILKVHYINVGQGDSILVQQDNENMLIDAGPNSSSEKLV
NYLKQNGIKEIKYLVATHPHEDHIGGMNKVINNFKVESVYMPKKIANTKTFKNMVEAMKKNNLKAKTPRVGDKINLGDAK
FTILWPNEQEQSNTNNYSIVLKGQYGENTFLFTGDAEQLAEKSILRNNTDIRAEVLKLGHHGSSTSTSDKFLEKVNPKYA
VASCEKGNSYGHPHREIVKKLKDKNIKLYRTDENGDIVLISDGKNIKFNVEPGSYNSGSSSKKK

Sequences:

>Translated_304_residues
MKKIRNSIKKISFIFIIVAMIFSMVACSEIGEQSSTSKSNNGILKVHYINVGQGDSILVQQDNENMLIDAGPNSSSEKLV
NYLKQNGIKEIKYLVATHPHEDHIGGMNKVINNFKVESVYMPKKIANTKTFKNMVEAMKKNNLKAKTPRVGDKINLGDAK
FTILWPNEQEQSNTNNYSIVLKGQYGENTFLFTGDAEQLAEKSILRNNTDIRAEVLKLGHHGSSTSTSDKFLEKVNPKYA
VASCEKGNSYGHPHREIVKKLKDKNIKLYRTDENGDIVLISDGKNIKFNVEPGSYNSGSSSKKK
>Mature_304_residues
MKKIRNSIKKISFIFIIVAMIFSMVACSEIGEQSSTSKSNNGILKVHYINVGQGDSILVQQDNENMLIDAGPNSSSEKLV
NYLKQNGIKEIKYLVATHPHEDHIGGMNKVINNFKVESVYMPKKIANTKTFKNMVEAMKKNNLKAKTPRVGDKINLGDAK
FTILWPNEQEQSNTNNYSIVLKGQYGENTFLFTGDAEQLAEKSILRNNTDIRAEVLKLGHHGSSTSTSDKFLEKVNPKYA
VASCEKGNSYGHPHREIVKKLKDKNIKLYRTDENGDIVLISDGKNIKFNVEPGSYNSGSSSKKK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=239, Percent_Identity=25.1046025104602, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 33977; Mature: 33977

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIRNSIKKISFIFIIVAMIFSMVACSEIGEQSSTSKSNNGILKVHYINVGQGDSILVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEE
QDNENMLIDAGPNSSSEKLVNYLKQNGIKEIKYLVATHPHEDHIGGMNKVINNFKVESVY
ECCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCHHHHHHCEEEEEEE
MPKKIANTKTFKNMVEAMKKNNLKAKTPRVGDKINLGDAKFTILWPNEQEQSNTNNYSIV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCEEEE
LKGQYGENTFLFTGDAEQLAEKSILRNNTDIRAEVLKLGHHGSSTSTSDKFLEKVNPKYA
EEECCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
VASCEKGNSYGHPHREIVKKLKDKNIKLYRTDENGDIVLISDGKNIKFNVEPGSYNSGSS
EEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCC
SKKK
CCCC
>Mature Secondary Structure
MKKIRNSIKKISFIFIIVAMIFSMVACSEIGEQSSTSKSNNGILKVHYINVGQGDSILVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEE
QDNENMLIDAGPNSSSEKLVNYLKQNGIKEIKYLVATHPHEDHIGGMNKVINNFKVESVY
ECCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCHHHHHHCEEEEEEE
MPKKIANTKTFKNMVEAMKKNNLKAKTPRVGDKINLGDAKFTILWPNEQEQSNTNNYSIV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCEEEE
LKGQYGENTFLFTGDAEQLAEKSILRNNTDIRAEVLKLGHHGSSTSTSDKFLEKVNPKYA
EEECCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
VASCEKGNSYGHPHREIVKKLKDKNIKLYRTDENGDIVLISDGKNIKFNVEPGSYNSGSS
EEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCC
SKKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]