Click here to switch to the map view.

The map label for this gene is asnA

Identifier: 28210498

GI number: 28210498

Start: 827494

End: 828519

Strand: Direct

Name: asnA

Synonym: CTC00781

Alternate gene names: 28210498

Gene position: 827494-828519 (Clockwise)

Preceding gene: 28210497

Following gene: 28210499

Centisome position: 29.56

GC content: 30.41

Gene sequence:

>1026_bases
ATGGAAATCAAATGTAGCGAAAGACTTATAATACCAAAAGAGTATAAAACTGAATTAGATTTAAAAGAAACTGCTATTGC
AATTAAGGAAGTGAAGGATTGTTTTGAAAGAGCTTTAGCAAAACAGCTAAATCTTATAAGAGTATCAGCACCTCTTTTTG
TAAGATGTGACAAGGGATTAAATGATAACTTAAATGGAGTAGAGAGACCAGTAAAGTTCACTGTGAAGGATGATAATGAA
GCAGCAGTAGAAATTGTTCATTCTCTAGCAAAGTGGAAGAGAATGGCTCTTTACAGATATAATTTTAATGCTGACGAAGG
TCTATATACAGATATGAATGCTATAAGAAGGGATGAGGAATTAGATAATACACATTCCATATATGTTGACCAATGGGATT
GGGAGAGAATAATAAAAAAAGAAGATAGAAATGAGGAATATTTAAAAGATATAGTTAGAAAAATATTTAAAGCTTTTAAA
GAAACAGAGGAGCATATAAACAAATTGTATCCATTTTTAGGAGAAGTTTTACCAGAAGAAGTATTTTTTATGACTACTCA
AGAACTTGAGGATATGTTTCCAGATCTTACAGCTAAAGAAAGAGAAGATGCTATAACTAAAGAAAAGAAAGCAGTATTTT
TAATGAAAATAGGAAAAACATTAGAATCAGGAGAAAAACATGATGGAAGAGCTCCAGACTATGATGACTGGGAATTAAAT
GGAGATATACTTTTTTGGAATCCAGTTCTAAATAAAGCTTTTGAATTATCTTCTATGGGAATAAGGGTAGATGAAGAATC
CCTTTTAAAACAATTAAAATTAGCTAATTGCGAAGAAAGAAAGGAATTACAATTTCATAAAATGCTTTTAGAGAAAAAAC
TACCATATACAATAGGTGGTGGAATAGGTCAATCTAGAATGTGTATGCTTTTCCTAAAAAAAGCTCATATAGGAGAGGTT
CAATCCTCAATATGGCCAGAAGAAATGATAAAGTTTTGTGAAGAAAAAGGAATGACAATATTATAA

Upstream 100 bases:

>100_bases
CTTAGTTGGGATGAAAGGCTTTTTTGATTAAAAAAATTTATTTACTAAAATCCATGAGGGTAAGTAGTTTATTATAGGAA
ATAATTTAGGAGGTAATTTT

Downstream 100 bases:

>100_bases
TATGAAAGTCTCTCTTTAATAAAAGAGGGACTTTTTATCGTATAGAAAGGTTTTTTAATATACATACTATAAATATATTG
TGGATTTAGTAAAATATTAT

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MEIKCSERLIIPKEYKTELDLKETAIAIKEVKDCFERALAKQLNLIRVSAPLFVRCDKGLNDNLNGVERPVKFTVKDDNE
AAVEIVHSLAKWKRMALYRYNFNADEGLYTDMNAIRRDEELDNTHSIYVDQWDWERIIKKEDRNEEYLKDIVRKIFKAFK
ETEEHINKLYPFLGEVLPEEVFFMTTQELEDMFPDLTAKEREDAITKEKKAVFLMKIGKTLESGEKHDGRAPDYDDWELN
GDILFWNPVLNKAFELSSMGIRVDEESLLKQLKLANCEERKELQFHKMLLEKKLPYTIGGGIGQSRMCMLFLKKAHIGEV
QSSIWPEEMIKFCEEKGMTIL

Sequences:

>Translated_341_residues
MEIKCSERLIIPKEYKTELDLKETAIAIKEVKDCFERALAKQLNLIRVSAPLFVRCDKGLNDNLNGVERPVKFTVKDDNE
AAVEIVHSLAKWKRMALYRYNFNADEGLYTDMNAIRRDEELDNTHSIYVDQWDWERIIKKEDRNEEYLKDIVRKIFKAFK
ETEEHINKLYPFLGEVLPEEVFFMTTQELEDMFPDLTAKEREDAITKEKKAVFLMKIGKTLESGEKHDGRAPDYDDWELN
GDILFWNPVLNKAFELSSMGIRVDEESLLKQLKLANCEERKELQFHKMLLEKKLPYTIGGGIGQSRMCMLFLKKAHIGEV
QSSIWPEEMIKFCEEKGMTIL
>Mature_341_residues
MEIKCSERLIIPKEYKTELDLKETAIAIKEVKDCFERALAKQLNLIRVSAPLFVRCDKGLNDNLNGVERPVKFTVKDDNE
AAVEIVHSLAKWKRMALYRYNFNADEGLYTDMNAIRRDEELDNTHSIYVDQWDWERIIKKEDRNEEYLKDIVRKIFKAFK
ETEEHINKLYPFLGEVLPEEVFFMTTQELEDMFPDLTAKEREDAITKEKKAVFLMKIGKTLESGEKHDGRAPDYDDWELN
GDILFWNPVLNKAFELSSMGIRVDEESLLKQLKLANCEERKELQFHKMLLEKKLPYTIGGGIGQSRMCMLFLKKAHIGEV
QSSIWPEEMIKFCEEKGMTIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=312, Percent_Identity=50.6410256410256, Blast_Score=308, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_CLOTE (Q897F7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781442.1
- ProteinModelPortal:   Q897F7
- SMR:   Q897F7
- GeneID:   1060794
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00781
- NMPDR:   fig|212717.1.peg.664
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- PhylomeDB:   Q897F7
- ProtClustDB:   PRK05425
- BioCyc:   CTET212717:CTC_00781-MONOMER
- BRENDA:   6.3.1.1
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 40006; Mature: 40006

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKCSERLIIPKEYKTELDLKETAIAIKEVKDCFERALAKQLNLIRVSAPLFVRCDKGL
CCCCCCCCEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCC
NDNLNGVERPVKFTVKDDNEAAVEIVHSLAKWKRMALYRYNFNADEGLYTDMNAIRRDEE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
LDNTHSIYVDQWDWERIIKKEDRNEEYLKDIVRKIFKAFKETEEHINKLYPFLGEVLPEE
CCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFFMTTQELEDMFPDLTAKEREDAITKEKKAVFLMKIGKTLESGEKHDGRAPDYDDWELN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEC
GDILFWNPVLNKAFELSSMGIRVDEESLLKQLKLANCEERKELQFHKMLLEKKLPYTIGG
CCEEEECHHHHHHHHHHHCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECC
GIGQSRMCMLFLKKAHIGEVQSSIWPEEMIKFCEEKGMTIL
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEIKCSERLIIPKEYKTELDLKETAIAIKEVKDCFERALAKQLNLIRVSAPLFVRCDKGL
CCCCCCCCEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCC
NDNLNGVERPVKFTVKDDNEAAVEIVHSLAKWKRMALYRYNFNADEGLYTDMNAIRRDEE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
LDNTHSIYVDQWDWERIIKKEDRNEEYLKDIVRKIFKAFKETEEHINKLYPFLGEVLPEE
CCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFFMTTQELEDMFPDLTAKEREDAITKEKKAVFLMKIGKTLESGEKHDGRAPDYDDWELN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEC
GDILFWNPVLNKAFELSSMGIRVDEESLLKQLKLANCEERKELQFHKMLLEKKLPYTIGG
CCEEEECHHHHHHHHHHHCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECC
GIGQSRMCMLFLKKAHIGEVQSSIWPEEMIKFCEEKGMTIL
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129