Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 28210451

GI number: 28210451

Start: 756210

End: 757478

Strand: Direct

Name: hemL

Synonym: CTC00730

Alternate gene names: 28210451

Gene position: 756210-757478 (Clockwise)

Preceding gene: 28210450

Following gene: 28210452

Centisome position: 27.01

GC content: 35.22

Gene sequence:

>1269_bases
GTGAATAATTTAGAAATATTTAAGGAATCTGAAAAATATATGCCAGGTGGAGTTAATAGTCCTGTAAGAGCTTTTAGCAA
TGCATCTATAAATCCACCAATAATAAAAAAAGGCTTAGGTTCTAGAATAGTTGATGAGGAAGGAAAAGAGTACATAGATT
TTGTAGCTTCCTGGGGACCTATGATATTAGGACATAATGATCCTGAAGTAGTAGAGGCTATACAAAATACTGTAGAAGAT
GCTATAAGTTTTGGTGCACCAACTGAATTAGAACTTAAACTAGCAAAGCACATGGTTGAAACCTTAGATAATATAGATAT
GGTTAGAATGGTAAACTCCGGCACAGAGGCTACTATGAGTGCTATAAAACTAGCAAGAGGATATACTAGAAGGGATAGAA
TAATAAAATTTGCTGGATGTTATCATGGGCACTTTGATGGATTTTTAGTTGAAGCGGGGTCAGGGGTATTAACAGAAAGA
ATACCAGGGTCACCAGGGGTGCCAAAAGGCAGTATAGAAAATACATTAATAGCTGAGTACAATCACATAGATACTGTAGA
GGCACTTTTTGAAAAATATCCAGAAGAAATAGCAGCAATAATAATAGAGCCTATTGCTGGAAATATGGGAACAATTCCAG
CTAAAACAGAATTTTTACAAAGACTTAGAGAAATTTGTACAAAATATGGAGCACTTTTAATATTCGATGAAGTTATGACT
GGATTTAGAGTGGCCTATAAAGGGGCACAAAGTAGATATGGAGTTAAACCAGATTTAACAACTTATGCAAAGATAATGGG
TGGTGGACTACCAAGCGGTGCCTATGGTGGAAGAAGAGATATTATGGAAAAGCTTTCACCAATAGGACCTGTATATCAAG
CGGGAACTATGTCAGGAAATCCAGTAGTTATGGCAGCAGGATATACTACTTTAAGGAAGCTTTATAATAATCCTTCCTAC
TATGAGCATATGGAGAAAATAGGTGCAGAACTTCAAAAGGGTATAGAAATAGTTGCAAAGGAAAAGGGACTTCCTGTAGT
GGTAAATAGATGTGGTGCTATGTTAACACCTTTCTTTAGCAAAAGAGATAAAGTAGCAGATTATGAAGATGCCAAGTCTT
CTGATACAGAGCTTTATGGAAGATTTTTTGAACACATGATAAAAAGCGGAATATATATAGCTCCATCACAATTTGAAGCT
ATGTTTATAGGAGTTAAACATGATATATATGAAGTTGAAAGATTCCTAGAAGCTATGAGAAAGTTTTAA

Upstream 100 bases:

>100_bases
GTAGCTTTAGAAATGCTTCTTTCTATAAAAAGAGCGGGAGCAGATATGATAATAACTTATTATGCTCTACAAGCATCAAA
ATGGTTAGGAGAGGATAAAT

Downstream 100 bases:

>100_bases
TAGGTGTAGATTATGAAAAGTATAGTTATATCATCTAATAGTAGTGGTGGAGGTAAAACCACCATTGCTGTTGGAATTAT
GAAAGCTTTAATGAAAAAAG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNNLEIFKESEKYMPGGVNSPVRAFSNASINPPIIKKGLGSRIVDEEGKEYIDFVASWGPMILGHNDPEVVEAIQNTVED
AISFGAPTELELKLAKHMVETLDNIDMVRMVNSGTEATMSAIKLARGYTRRDRIIKFAGCYHGHFDGFLVEAGSGVLTER
IPGSPGVPKGSIENTLIAEYNHIDTVEALFEKYPEEIAAIIIEPIAGNMGTIPAKTEFLQRLREICTKYGALLIFDEVMT
GFRVAYKGAQSRYGVKPDLTTYAKIMGGGLPSGAYGGRRDIMEKLSPIGPVYQAGTMSGNPVVMAAGYTTLRKLYNNPSY
YEHMEKIGAELQKGIEIVAKEKGLPVVVNRCGAMLTPFFSKRDKVADYEDAKSSDTELYGRFFEHMIKSGIYIAPSQFEA
MFIGVKHDIYEVERFLEAMRKF

Sequences:

>Translated_422_residues
MNNLEIFKESEKYMPGGVNSPVRAFSNASINPPIIKKGLGSRIVDEEGKEYIDFVASWGPMILGHNDPEVVEAIQNTVED
AISFGAPTELELKLAKHMVETLDNIDMVRMVNSGTEATMSAIKLARGYTRRDRIIKFAGCYHGHFDGFLVEAGSGVLTER
IPGSPGVPKGSIENTLIAEYNHIDTVEALFEKYPEEIAAIIIEPIAGNMGTIPAKTEFLQRLREICTKYGALLIFDEVMT
GFRVAYKGAQSRYGVKPDLTTYAKIMGGGLPSGAYGGRRDIMEKLSPIGPVYQAGTMSGNPVVMAAGYTTLRKLYNNPSY
YEHMEKIGAELQKGIEIVAKEKGLPVVVNRCGAMLTPFFSKRDKVADYEDAKSSDTELYGRFFEHMIKSGIYIAPSQFEA
MFIGVKHDIYEVERFLEAMRKF
>Mature_422_residues
MNNLEIFKESEKYMPGGVNSPVRAFSNASINPPIIKKGLGSRIVDEEGKEYIDFVASWGPMILGHNDPEVVEAIQNTVED
AISFGAPTELELKLAKHMVETLDNIDMVRMVNSGTEATMSAIKLARGYTRRDRIIKFAGCYHGHFDGFLVEAGSGVLTER
IPGSPGVPKGSIENTLIAEYNHIDTVEALFEKYPEEIAAIIIEPIAGNMGTIPAKTEFLQRLREICTKYGALLIFDEVMT
GFRVAYKGAQSRYGVKPDLTTYAKIMGGGLPSGAYGGRRDIMEKLSPIGPVYQAGTMSGNPVVMAAGYTTLRKLYNNPSY
YEHMEKIGAELQKGIEIVAKEKGLPVVVNRCGAMLTPFFSKRDKVADYEDAKSSDTELYGRFFEHMIKSGIYIAPSQFEA
MFIGVKHDIYEVERFLEAMRKF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=318, Percent_Identity=28.3018867924528, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI37574042, Length=336, Percent_Identity=26.1904761904762, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI226442705, Length=337, Percent_Identity=25.2225519287834, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI226442709, Length=304, Percent_Identity=26.6447368421053, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI284507298, Length=238, Percent_Identity=28.9915966386555, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786349, Length=416, Percent_Identity=52.1634615384615, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789016, Length=374, Percent_Identity=29.6791443850267, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788044, Length=302, Percent_Identity=36.7549668874172, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789759, Length=360, Percent_Identity=30.5555555555556, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1787560, Length=377, Percent_Identity=28.9124668435013, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI145693181, Length=315, Percent_Identity=30.7936507936508, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786991, Length=289, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71992977, Length=344, Percent_Identity=27.3255813953488, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=27.5747508305648, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=340, Percent_Identity=27.9411764705882, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323470, Length=288, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324386, Length=282, Percent_Identity=24.468085106383, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357415, Length=338, Percent_Identity=27.5147928994083, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356575, Length=304, Percent_Identity=26.9736842105263, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CLOTE (Q897K4)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781395.1
- ProteinModelPortal:   Q897K4
- SMR:   Q897K4
- GeneID:   1060808
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00730
- NMPDR:   fig|212717.1.peg.617
- HOGENOM:   HBG725944
- OMA:   FYTASAN
- PhylomeDB:   Q897K4
- ProtClustDB:   PRK00062
- BioCyc:   CTET212717:CTC_00730-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46725; Mature: 46725

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLEIFKESEKYMPGGVNSPVRAFSNASINPPIIKKGLGSRIVDEEGKEYIDFVASWGP
CCCCHHEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCHHHHHCHHHHHHHHHHCCC
MILGHNDPEVVEAIQNTVEDAISFGAPTELELKLAKHMVETLDNIDMVRMVNSGTEATMS
EEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
AIKLARGYTRRDRIIKFAGCYHGHFDGFLVEAGSGVLTERIPGSPGVPKGSIENTLIAEY
HHHHHHCCHHHHHHEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCEEECC
NHIDTVEALFEKYPEEIAAIIIEPIAGNMGTIPAKTEFLQRLREICTKYGALLIFDEVMT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
GFRVAYKGAQSRYGVKPDLTTYAKIMGGGLPSGAYGGRRDIMEKLSPIGPVYQAGTMSGN
HHHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCC
PVVMAAGYTTLRKLYNNPSYYEHMEKIGAELQKGIEIVAKEKGLPVVVNRCGAMLTPFFS
CEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRDKVADYEDAKSSDTELYGRFFEHMIKSGIYIAPSQFEAMFIGVKHDIYEVERFLEAMR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
KF
CC
>Mature Secondary Structure
MNNLEIFKESEKYMPGGVNSPVRAFSNASINPPIIKKGLGSRIVDEEGKEYIDFVASWGP
CCCCHHEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCHHHHHCHHHHHHHHHHCCC
MILGHNDPEVVEAIQNTVEDAISFGAPTELELKLAKHMVETLDNIDMVRMVNSGTEATMS
EEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
AIKLARGYTRRDRIIKFAGCYHGHFDGFLVEAGSGVLTERIPGSPGVPKGSIENTLIAEY
HHHHHHCCHHHHHHEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCEEECC
NHIDTVEALFEKYPEEIAAIIIEPIAGNMGTIPAKTEFLQRLREICTKYGALLIFDEVMT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
GFRVAYKGAQSRYGVKPDLTTYAKIMGGGLPSGAYGGRRDIMEKLSPIGPVYQAGTMSGN
HHHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCC
PVVMAAGYTTLRKLYNNPSYYEHMEKIGAELQKGIEIVAKEKGLPVVVNRCGAMLTPFFS
CEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRDKVADYEDAKSSDTELYGRFFEHMIKSGIYIAPSQFEAMFIGVKHDIYEVERFLEAMR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
KF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129