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The map label for this gene is cobB

Identifier: 28210452

GI number: 28210452

Start: 757491

End: 758810

Strand: Direct

Name: cobB

Synonym: CTC00731

Alternate gene names: 28210452

Gene position: 757491-758810 (Clockwise)

Preceding gene: 28210451

Following gene: 28210453

Centisome position: 27.06

GC content: 28.79

Gene sequence:

>1320_bases
ATGAAAAGTATAGTTATATCATCTAATAGTAGTGGTGGAGGTAAAACCACCATTGCTGTTGGAATTATGAAAGCTTTAAT
GAAAAAAGGGTTTGATGTTCAAGGATATAAGGTTGGACCAGACTATATAGATCCAGCTTTTCATAGCCAAATAACTGGAA
AACCTTCTAGAAATTTAGATATATACCTTACGGGAGAAGAAGGAGTAAAAGCTTCTTATTCCAGAGGGAAAGGAAATTTA
GGTGTTGTAGAAGGAGTTATGGGCCTATATGATGGAAAAGGCATAGATTCTAAATATTCTACAGCTCATGTAGCAAAAAC
ACTAGGGTTGCCTGTGGTTTTAGTATTATCGCCTAAAGCTCAAAGTGCAACTTTATGTGCAGAAATAAATGGACTTATAA
ATTTTGATAAAGTTGAAATAGGTGGAATAATCTTAAACAATATATCAGAAAGTTATTATAATCTTTTAAAAGCTGCTATA
GAATATAACTGCAATGCTAAGGTTTTAGGATATATTCCAAAAGACGAAAGATTAAAAATAGGAAGTAGACATTTAGGACT
TATACAAAGTAGTGAAATAGAAGACTTAGAAGAAAAAATAGATATATGTAGTGAACATATATTAAAAAATATAGATTTAG
AGGAACTTTTTAAAATATTTAAAAAAACTCCTGAATATGAAGATAACTTTCACTTGGAAAATAAAAATTTAAAAATAGCA
GTAGCTTATGATAAGGCTTTCTCATTTTATTATAGAGATAATATAGAGCTTTTAGAGGAGTTAGGAGAAATTACTTATTT
TAGTCCATTGATGGATAAAGAACTGCCTAAAGATATAGATTTTCTTTATATAGGTGGAGGATATCCAGAAGTATTTATAG
AAGAGCTTTCTAAAAATGAATCTATGTTAAAAAGTATAAAAGAAGAATTAGATAAAGGACTTAAATGTTATGCAGAATGT
GGAGGTCTTATGTACCTTACAGAAGCTATAGAAAATGAAGATTTAAAAGGAAATGCCGTAGGATATTTTGAAGGATTTTC
CAAAATGACAAAAAGACTTCAAAACTTTGGATATGCTCAGTTAGAAGTTTATAAGGAAAATGAAATACTTCCTAAAGGTC
TTAAAATAAATTGTCATGAATTTCATAAATCCACTGTGGAATTAGATGAAGATAAAATATTTAAAATAAATAAAACTATG
TATAATGGTGACACAAAACATTGGAATTGCGGATATATAAAGGGTAATACCATAGCAGCCTATGCCCATGTAAATTTCCT
AGGAAATATAGAATTTTTAAAAGCTTTATTAGAAAAATAA

Upstream 100 bases:

>100_bases
TCCATCACAATTTGAAGCTATGTTTATAGGAGTTAAACATGATATATATGAAGTTGAAAGATTCCTAGAAGCTATGAGAA
AGTTTTAATAGGTGTAGATT

Downstream 100 bases:

>100_bases
AAATGGTTATGAAAGAAGGGATTAAAGTGATTGAATATATAAAAAAACCTATGGCAATAGAAGAAAAAAGTTTTGAAATA
ATAGGAAGCGAAATGAAAAG

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKSIVISSNSSGGGKTTIAVGIMKALMKKGFDVQGYKVGPDYIDPAFHSQITGKPSRNLDIYLTGEEGVKASYSRGKGNL
GVVEGVMGLYDGKGIDSKYSTAHVAKTLGLPVVLVLSPKAQSATLCAEINGLINFDKVEIGGIILNNISESYYNLLKAAI
EYNCNAKVLGYIPKDERLKIGSRHLGLIQSSEIEDLEEKIDICSEHILKNIDLEELFKIFKKTPEYEDNFHLENKNLKIA
VAYDKAFSFYYRDNIELLEELGEITYFSPLMDKELPKDIDFLYIGGGYPEVFIEELSKNESMLKSIKEELDKGLKCYAEC
GGLMYLTEAIENEDLKGNAVGYFEGFSKMTKRLQNFGYAQLEVYKENEILPKGLKINCHEFHKSTVELDEDKIFKINKTM
YNGDTKHWNCGYIKGNTIAAYAHVNFLGNIEFLKALLEK

Sequences:

>Translated_439_residues
MKSIVISSNSSGGGKTTIAVGIMKALMKKGFDVQGYKVGPDYIDPAFHSQITGKPSRNLDIYLTGEEGVKASYSRGKGNL
GVVEGVMGLYDGKGIDSKYSTAHVAKTLGLPVVLVLSPKAQSATLCAEINGLINFDKVEIGGIILNNISESYYNLLKAAI
EYNCNAKVLGYIPKDERLKIGSRHLGLIQSSEIEDLEEKIDICSEHILKNIDLEELFKIFKKTPEYEDNFHLENKNLKIA
VAYDKAFSFYYRDNIELLEELGEITYFSPLMDKELPKDIDFLYIGGGYPEVFIEELSKNESMLKSIKEELDKGLKCYAEC
GGLMYLTEAIENEDLKGNAVGYFEGFSKMTKRLQNFGYAQLEVYKENEILPKGLKINCHEFHKSTVELDEDKIFKINKTM
YNGDTKHWNCGYIKGNTIAAYAHVNFLGNIEFLKALLEK
>Mature_439_residues
MKSIVISSNSSGGGKTTIAVGIMKALMKKGFDVQGYKVGPDYIDPAFHSQITGKPSRNLDIYLTGEEGVKASYSRGKGNL
GVVEGVMGLYDGKGIDSKYSTAHVAKTLGLPVVLVLSPKAQSATLCAEINGLINFDKVEIGGIILNNISESYYNLLKAAI
EYNCNAKVLGYIPKDERLKIGSRHLGLIQSSEIEDLEEKIDICSEHILKNIDLEELFKIFKKTPEYEDNFHLENKNLKIA
VAYDKAFSFYYRDNIELLEELGEITYFSPLMDKELPKDIDFLYIGGGYPEVFIEELSKNESMLKSIKEELDKGLKCYAEC
GGLMYLTEAIENEDLKGNAVGYFEGFSKMTKRLQNFGYAQLEVYKENEILPKGLKINCHEFHKSTVELDEDKIFKINKTM
YNGDTKHWNCGYIKGNTIAAYAHVNFLGNIEFLKALLEK

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_CLOTE (Q897K3)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781396.1
- ProteinModelPortal:   Q897K3
- GeneID:   1059664
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00731
- NMPDR:   fig|212717.1.peg.618
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   PRK01077
- BioCyc:   CTET212717:CTC_00731-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: NA

Molecular weight: Translated: 49203; Mature: 49203

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 320-320 ACT_SITE 423-423

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIVISSNSSGGGKTTIAVGIMKALMKKGFDVQGYKVGPDYIDPAFHSQITGKPSRNLD
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEE
IYLTGEEGVKASYSRGKGNLGVVEGVMGLYDGKGIDSKYSTAHVAKTLGLPVVLVLSPKA
EEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
QSATLCAEINGLINFDKVEIGGIILNNISESYYNLLKAAIEYNCNAKVLGYIPKDERLKI
CCCHHHHHHCCCEEECEEEECCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEC
GSRHLGLIQSSEIEDLEEKIDICSEHILKNIDLEELFKIFKKTPEYEDNFHLENKNLKIA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEECCCEEEE
VAYDKAFSFYYRDNIELLEELGEITYFSPLMDKELPKDIDFLYIGGGYPEVFIEELSKNE
EEEECEEEEEECCCHHHHHHHHHHEEECHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCH
SMLKSIKEELDKGLKCYAECGGLMYLTEAIENEDLKGNAVGYFEGFSKMTKRLQNFGYAQ
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEE
LEVYKENEILPKGLKINCHEFHKSTVELDEDKIFKINKTMYNGDTKHWNCGYIKGNTIAA
EEEEECCCCCCCCCEEEHHHHCCCCEECCCCCEEEEECEEECCCCCEEEEEEEECCEEEE
YAHVNFLGNIEFLKALLEK
EEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKSIVISSNSSGGGKTTIAVGIMKALMKKGFDVQGYKVGPDYIDPAFHSQITGKPSRNLD
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEE
IYLTGEEGVKASYSRGKGNLGVVEGVMGLYDGKGIDSKYSTAHVAKTLGLPVVLVLSPKA
EEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
QSATLCAEINGLINFDKVEIGGIILNNISESYYNLLKAAIEYNCNAKVLGYIPKDERLKI
CCCHHHHHHCCCEEECEEEECCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEC
GSRHLGLIQSSEIEDLEEKIDICSEHILKNIDLEELFKIFKKTPEYEDNFHLENKNLKIA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEECCCEEEE
VAYDKAFSFYYRDNIELLEELGEITYFSPLMDKELPKDIDFLYIGGGYPEVFIEELSKNE
EEEECEEEEEECCCHHHHHHHHHHEEECHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCH
SMLKSIKEELDKGLKCYAECGGLMYLTEAIENEDLKGNAVGYFEGFSKMTKRLQNFGYAQ
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEE
LEVYKENEILPKGLKINCHEFHKSTVELDEDKIFKINKTMYNGDTKHWNCGYIKGNTIAA
EEEEECCCCCCCCCEEEHHHHCCCCEECCCCCEEEEECEEECCCCCEEEEEEEECCEEEE
YAHVNFLGNIEFLKALLEK
EEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129