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The map label for this gene is hemD [H]

Identifier: 28210449

GI number: 28210449

Start: 753763

End: 755220

Strand: Direct

Name: hemD [H]

Synonym: CTC00728

Alternate gene names: 28210449

Gene position: 753763-755220 (Clockwise)

Preceding gene: 161486849

Following gene: 28210450

Centisome position: 26.93

GC content: 30.18

Gene sequence:

>1458_bases
ATGGGTAAGGTATATCTAATAGGGGCAGGACCAGGAGATGAAGAACTTATAACATTAAAAGCCACTAGAATGTTGCAAAA
GTGCACTGCTGTTATGTATGACAGATTAGCAGGGCCAGGATTCTTAAAATATTTAAATGAGGATTGTGAGATTTATTACT
GTGGTAAGGAACCAGGTTGTCATTATAAAACTCAAGATGAGATAAATGAAATGCTTGCAAAGCTAGCTAAAAAAGGTCAC
GTAGTTGGAAGAGTAAAAGGTGGAGATCCTTTTATATTTGGACGTGGAGGAGAAGAAATATTAAGACTTTTAGAAGATAA
TATAGAGTTTGAAGTAATACCAGGAATAACTTCAGCAATATCCGTTCCAAGCTATGCTGGAATTCCAGTAACTCATAGAG
CTATTGCTCAAGGTTTTCATGTATTTACAGGAATGACAGCAGAAAAACTTGATATAGATTGGAAAGCTGTTGCAAATGTA
GGGGGAACTTTAATATTTTTAATGGGTTATAGAAATTTAGATATAATTCGCAATAGATTAATGGAAAATGGAATGAATGA
AAATACTCCTTGTGCAATTATAATGAGAGGAACTACTTCTAATCAAAAAAAGGTAGTGGGTACTTTAAAAGATATTTCAG
AAAAAGCAGAGAAACAAGGTGTAACTTCTCCTTGTATAATACTAATTGGAGATGTAGTACAATTTAATGATAAATTTAAT
TGGTATGAAAAGAAAAGACTTTTTGGCAAAAATATATGTGTAACTCGTTCAAGAGTTCAGGCAAAAGAATTAAGAGAAAA
GTTATCAGATTTAGGAGCACAGGTTACAGAGATAAATGCTATCAAAATAAAAAGTAGAGACGATGAGTTAGAAAAATATG
TAGAAAGATTAAAAGATTATAAATTTATAGTTTTAACAAGTGTAAATGCGGTAAATATACTTTTCGATTATCTAGTAAAA
AAAGAATATGATATAAGAAATATAAAAGGAAGATTTGCAGTAATAGGGCCTGCAACAGAAAAAGCATTATTAAAAAGAGG
TATAAAAGCGGATATAATTGCTAAAGAGTTTGTAGCGGAAGATTTATTTAATGAATTAAAACCTTATTTAAATAAGGATG
ATAAAATATTTGTACCTCATTCTAAACAAGCTAGACAGTATTTAGTAGAAGCCCTAAAAGATAAAGGGTGCCATGTAGAA
GAAGTCTATAGTTATGAAGTGGTTAAAGGAGAAGTTCCTAATTTAGAAATATTTGATGATGTAGATATAGTTTTATATAC
TAGTCCAAGTATAGTTAGAAACATGATAGATATGATAGGTTTAGAAAAACTTAAGAAAAAGACTTCAATATCCATAGGAC
CTATAACAGCCAAGGAACTAGATAAAAATGGTATAGATTATATTATCTGTGATGAATATACTACAGATGGAATGATAGAA
AAACTTTTAACTTTATAA

Upstream 100 bases:

>100_bases
AAAAATTGTAAAGAAAGATATTGAAGGTAAGAAAGAAAATTATAAACAACTAGGAAAAACATTAGCAGATAGAATAATGA
AGGCTTAAGGAGGTAAAAGT

Downstream 100 bases:

>100_bases
AGAGGATGTGAAAATTATGAATTTTAGAAGACAAAGAAGACTTAGAAAAAATGAAGCTATAAGAAGTATGGTAAGGGAAA
CTATATTAACTACAAATGAC

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGKVYLIGAGPGDEELITLKATRMLQKCTAVMYDRLAGPGFLKYLNEDCEIYYCGKEPGCHYKTQDEINEMLAKLAKKGH
VVGRVKGGDPFIFGRGGEEILRLLEDNIEFEVIPGITSAISVPSYAGIPVTHRAIAQGFHVFTGMTAEKLDIDWKAVANV
GGTLIFLMGYRNLDIIRNRLMENGMNENTPCAIIMRGTTSNQKKVVGTLKDISEKAEKQGVTSPCIILIGDVVQFNDKFN
WYEKKRLFGKNICVTRSRVQAKELREKLSDLGAQVTEINAIKIKSRDDELEKYVERLKDYKFIVLTSVNAVNILFDYLVK
KEYDIRNIKGRFAVIGPATEKALLKRGIKADIIAKEFVAEDLFNELKPYLNKDDKIFVPHSKQARQYLVEALKDKGCHVE
EVYSYEVVKGEVPNLEIFDDVDIVLYTSPSIVRNMIDMIGLEKLKKKTSISIGPITAKELDKNGIDYIICDEYTTDGMIE
KLLTL

Sequences:

>Translated_485_residues
MGKVYLIGAGPGDEELITLKATRMLQKCTAVMYDRLAGPGFLKYLNEDCEIYYCGKEPGCHYKTQDEINEMLAKLAKKGH
VVGRVKGGDPFIFGRGGEEILRLLEDNIEFEVIPGITSAISVPSYAGIPVTHRAIAQGFHVFTGMTAEKLDIDWKAVANV
GGTLIFLMGYRNLDIIRNRLMENGMNENTPCAIIMRGTTSNQKKVVGTLKDISEKAEKQGVTSPCIILIGDVVQFNDKFN
WYEKKRLFGKNICVTRSRVQAKELREKLSDLGAQVTEINAIKIKSRDDELEKYVERLKDYKFIVLTSVNAVNILFDYLVK
KEYDIRNIKGRFAVIGPATEKALLKRGIKADIIAKEFVAEDLFNELKPYLNKDDKIFVPHSKQARQYLVEALKDKGCHVE
EVYSYEVVKGEVPNLEIFDDVDIVLYTSPSIVRNMIDMIGLEKLKKKTSISIGPITAKELDKNGIDYIICDEYTTDGMIE
KLLTL
>Mature_484_residues
GKVYLIGAGPGDEELITLKATRMLQKCTAVMYDRLAGPGFLKYLNEDCEIYYCGKEPGCHYKTQDEINEMLAKLAKKGHV
VGRVKGGDPFIFGRGGEEILRLLEDNIEFEVIPGITSAISVPSYAGIPVTHRAIAQGFHVFTGMTAEKLDIDWKAVANVG
GTLIFLMGYRNLDIIRNRLMENGMNENTPCAIIMRGTTSNQKKVVGTLKDISEKAEKQGVTSPCIILIGDVVQFNDKFNW
YEKKRLFGKNICVTRSRVQAKELREKLSDLGAQVTEINAIKIKSRDDELEKYVERLKDYKFIVLTSVNAVNILFDYLVKK
EYDIRNIKGRFAVIGPATEKALLKRGIKADIIAKEFVAEDLFNELKPYLNKDDKIFVPHSKQARQYLVEALKDKGCHVEE
VYSYEVVKGEVPNLEIFDDVDIVLYTSPSIVRNMIDMIGLEKLKKKTSISIGPITAKELDKNGIDYIICDEYTTDGMIEK
LLTL

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=42.5619834710744, Blast_Score=205, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322922, Length=250, Percent_Identity=28.8, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54625; Mature: 54494

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVYLIGAGPGDEELITLKATRMLQKCTAVMYDRLAGPGFLKYLNEDCEIYYCGKEPGC
CCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCC
HYKTQDEINEMLAKLAKKGHVVGRVKGGDPFIFGRGGEEILRLLEDNIEFEVIPGITSAI
CCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHHHHCCCCEEEECCCHHHH
SVPSYAGIPVTHRAIAQGFHVFTGMTAEKLDIDWKAVANVGGTLIFLMGYRNLDIIRNRL
CCCCCCCCCCHHHHHHCCHHHHCCCCHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHH
MENGMNENTPCAIIMRGTTSNQKKVVGTLKDISEKAEKQGVTSPCIILIGDVVQFNDKFN
HHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEECCCCC
WYEKKRLFGKNICVTRSRVQAKELREKLSDLGAQVTEINAIKIKSRDDELEKYVERLKDY
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCC
KFIVLTSVNAVNILFDYLVKKEYDIRNIKGRFAVIGPATEKALLKRGIKADIIAKEFVAE
EEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHH
DLFNELKPYLNKDDKIFVPHSKQARQYLVEALKDKGCHVEEVYSYEVVKGEVPNLEIFDD
HHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCEEEECC
VDIVLYTSPSIVRNMIDMIGLEKLKKKTSISIGPITAKELDKNGIDYIICDEYTTDGMIE
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHCCCCCCEEEECCCCCCHHHH
KLLTL
HHHCC
>Mature Secondary Structure 
GKVYLIGAGPGDEELITLKATRMLQKCTAVMYDRLAGPGFLKYLNEDCEIYYCGKEPGC
CEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCC
HYKTQDEINEMLAKLAKKGHVVGRVKGGDPFIFGRGGEEILRLLEDNIEFEVIPGITSAI
CCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHHHHCCCCEEEECCCHHHH
SVPSYAGIPVTHRAIAQGFHVFTGMTAEKLDIDWKAVANVGGTLIFLMGYRNLDIIRNRL
CCCCCCCCCCHHHHHHCCHHHHCCCCHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHH
MENGMNENTPCAIIMRGTTSNQKKVVGTLKDISEKAEKQGVTSPCIILIGDVVQFNDKFN
HHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEECCCCC
WYEKKRLFGKNICVTRSRVQAKELREKLSDLGAQVTEINAIKIKSRDDELEKYVERLKDY
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCC
KFIVLTSVNAVNILFDYLVKKEYDIRNIKGRFAVIGPATEKALLKRGIKADIIAKEFVAE
EEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHH
DLFNELKPYLNKDDKIFVPHSKQARQYLVEALKDKGCHVEEVYSYEVVKGEVPNLEIFDD
HHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCEEEECC
VDIVLYTSPSIVRNMIDMIGLEKLKKKTSISIGPITAKELDKNGIDYIICDEYTTDGMIE
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHCCCCCCEEEECCCCCCHHHH
KLLTL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]