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The map label for this gene is cobS

Identifier: 28210440

GI number: 28210440

Start: 744531

End: 745289

Strand: Direct

Name: cobS

Synonym: CTC00719

Alternate gene names: 28210440

Gene position: 744531-745289 (Clockwise)

Preceding gene: 28210439

Following gene: 28210441

Centisome position: 26.6

GC content: 26.75

Gene sequence:

>759_bases
GTGAAATACATATATAATTTTTTATTAATGATACAATTTTTAACAAGAATTCCTGTAAAAAGGAGTTTACCCTGTGAAAA
AGAGGATTTCAGAAGAGGAGCAGCCATGCTACCGCTAATAGGACTAATTGTAGGCTGCATTCAATGGGTAGTTTTTTATA
TTTTATCTAAAATATTCCCAGCAAATATAACAGCTATATTTATAATATTAGTAGGAATGGTGTTAATAGGAGGACTTCAT
CAAGATGGATTAGGAGACATATTTGATGGATTTTTCTCTTTTAAAGGTGACAAAGAAAAGATAATAGAAATAATGAAAGA
TAGTAGAGTTGGAACTTTTGCTGTACTAGCATTAATATTTGATATACTAATAAAATATTCAGCATTAAGTTTTATTATAG
AAAATAACATGTCCTATGCAATAATAATTACACCTATAATGAGTAGATGTACACTAGTTTTTCTTTTCTTAATAGGTAAG
AATGCTAAAAAAAATGGCACTGGAAATCTATTCATAGAAAATGTGTCAGTTAAAGAATTTATTATTAGTTTTATCTTTAT
GATAGTACCATCTGTATTATTAATAGGATATAAATATTCTGTAATAATAATTGTTGTAAGTTTTATAATAACTTTAGCGT
TTTTAAATTTATGTAATAGGAAAATAGGGGGAATTACAGGGGATTGTTTAGGTGCTAATAATGAGATTGTGGAAATGTTT
ACTATGCTAGTTTTTGTTGCTTTGCTTTACATAAATTAA

Upstream 100 bases:

>100_bases
CATTGCAGGATTTGTAAATCAGCACATAGGTAAAAAAGCAGATGAAATTTATTTAGTTTGTTGCGGACAACCGATGAAAA
TAAAATAGGGAGATGACATT

Downstream 100 bases:

>100_bases
ATATAAAAAAATGTTGACAAAATCAAATTCATATATTAGACTTATATTTGAAAAGTGAATATGAAAATATTCAGGTGCCA
TTTTAGGTTTAAAAGGGAAT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKYIYNFLLMIQFLTRIPVKRSLPCEKEDFRRGAAMLPLIGLIVGCIQWVVFYILSKIFPANITAIFIILVGMVLIGGLH
QDGLGDIFDGFFSFKGDKEKIIEIMKDSRVGTFAVLALIFDILIKYSALSFIIENNMSYAIIITPIMSRCTLVFLFLIGK
NAKKNGTGNLFIENVSVKEFIISFIFMIVPSVLLIGYKYSVIIIVVSFIITLAFLNLCNRKIGGITGDCLGANNEIVEMF
TMLVFVALLYIN

Sequences:

>Translated_252_residues
MKYIYNFLLMIQFLTRIPVKRSLPCEKEDFRRGAAMLPLIGLIVGCIQWVVFYILSKIFPANITAIFIILVGMVLIGGLH
QDGLGDIFDGFFSFKGDKEKIIEIMKDSRVGTFAVLALIFDILIKYSALSFIIENNMSYAIIITPIMSRCTLVFLFLIGK
NAKKNGTGNLFIENVSVKEFIISFIFMIVPSVLLIGYKYSVIIIVVSFIITLAFLNLCNRKIGGITGDCLGANNEIVEMF
TMLVFVALLYIN
>Mature_252_residues
MKYIYNFLLMIQFLTRIPVKRSLPCEKEDFRRGAAMLPLIGLIVGCIQWVVFYILSKIFPANITAIFIILVGMVLIGGLH
QDGLGDIFDGFFSFKGDKEKIIEIMKDSRVGTFAVLALIFDILIKYSALSFIIENNMSYAIIITPIMSRCTLVFLFLIGK
NAKKNGTGNLFIENVSVKEFIISFIFMIVPSVLLIGYKYSVIIIVVSFIITLAFLNLCNRKIGGITGDCLGANNEIVEMF
TMLVFVALLYIN

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=244, Percent_Identity=34.0163934426229, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CLOTE (Q897L5)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781384.1
- ProteinModelPortal:   Q897L5
- GeneID:   1059249
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00719
- NMPDR:   fig|212717.1.peg.606
- HOGENOM:   HBG729392
- OMA:   IMKDPTS
- PhylomeDB:   Q897L5
- ProtClustDB:   PRK00235
- BioCyc:   CTET212717:CTC_00719-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28306; Mature: 28306

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd142b90)-; HASH(0xd511c28)-; HASH(0xc980ccc)-; HASH(0xd46fe70)-; HASH(0xd0a858c)-; HASH(0xc974f48)-; HASH(0xd30e088)-;

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIYNFLLMIQFLTRIPVKRSLPCEKEDFRRGAAMLPLIGLIVGCIQWVVFYILSKIFP
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ANITAIFIILVGMVLIGGLHQDGLGDIFDGFFSFKGDKEKIIEIMKDSRVGTFAVLALIF
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
DILIKYSALSFIIENNMSYAIIITPIMSRCTLVFLFLIGKNAKKNGTGNLFIENVSVKEF
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHH
IISFIFMIVPSVLLIGYKYSVIIIVVSFIITLAFLNLCNRKIGGITGDCLGANNEIVEMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
TMLVFVALLYIN
HHHHHHHHHHCH
>Mature Secondary Structure
MKYIYNFLLMIQFLTRIPVKRSLPCEKEDFRRGAAMLPLIGLIVGCIQWVVFYILSKIFP
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ANITAIFIILVGMVLIGGLHQDGLGDIFDGFFSFKGDKEKIIEIMKDSRVGTFAVLALIF
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
DILIKYSALSFIIENNMSYAIIITPIMSRCTLVFLFLIGKNAKKNGTGNLFIENVSVKEF
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHH
IISFIFMIVPSVLLIGYKYSVIIIVVSFIITLAFLNLCNRKIGGITGDCLGANNEIVEMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
TMLVFVALLYIN
HHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12552129