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The map label for this gene is cbiP

Identifier: 28210441

GI number: 28210441

Start: 745746

End: 747221

Strand: Direct

Name: cbiP

Synonym: CTC00720

Alternate gene names: 28210441

Gene position: 745746-747221 (Clockwise)

Preceding gene: 28210440

Following gene: 28210442

Centisome position: 26.64

GC content: 29.27

Gene sequence:

>1476_bases
GTGTTTAAATTGGCAAAAATAATGATACAGGGGACAGGTTCCTCAGTAGGTAAAAGTATTTTAGTAACAGCATTATGTAG
AATATTTAAGCAAGATGGATATACTGTTTGTCCATATAAATCACAGAACATGGCTTTAAATTCATATATAACGTATGATG
GAAAAGAAATGGGAAGAGCACAAGTATTACAGGCTCACGCTGCAGGACTAGAACCAGAAGCATATATGAATCCTATACTT
TTAAAACCTACAGGGGATAAAAAATGTCAAATAATATTTAACGGCGAAGTTTATGGAAACAGTACAGCCATGGAATATCA
TAATATGAAAACTAAATTTGGTGAGAAGTTAAAAGATCAATTTAAAAATATAGAAGAAAAATTTGATATCGTAGTAATAG
AAGGTGCAGGTAGTCCAGCGGAAATAAATTTAAGGGATAAAGATATTGTAAATATGGGCTTTGCAGAAATGGTAGATGCA
CCAGTACTATTAGCAGGAGATATAGATAGAGGTGGAGTTTTTGCATCTTTAGCGGGAACTATGCTTCTTTTAACTGAAGA
AGAGAGGAATAGAGTTAAGGGAACTATAATAAATAAATTTAGAGGAGATATAGAAATTTTAAAGCCAGGCTTAAAAATGC
TAGAAGATATTATAAAAATACCTACAGTAGGAGTAGTTCCTTATTTAAAATTTCAGCTAGAAGATGAAGATGGAGCTATT
GATTTTAATAGAAATATTAATGCTCCTATAGATATAGCAGTAATAAAACTTCCACGTATATCTAATTTTACTGATTTTGA
TGCTTTAAAAGTAGAAGAAGATGTTTCAATTAGATATATAACTTCAAAAGAAGATTTTGGAAAACCTGATTTATTAATAG
TGCCAGGAAGTAAAAATACTATAGAAGATTTATTGGCACTAAGAAAATGTGGACTAGAGGAAAAAATAAAAGAATATAGT
AAAACAGGCACTATTATAGGAATTTGTGGAGGATATCAAATGCTTGGTAAAACTCTAAAGGATCCTTATAAAGTTGAATC
AGAAGAATTAGAAACACAAGGTATGGGACTTTTAAATGTGGATACAGTTTTTGAAAAAGAAAAAGTAACTACTAGAGTAA
AAGCTAATTCAGAGGATACAGAAGTTTATGGATATGAAATACATATGGGAATATGCAACTATAAAGAAGGAGCTAAGCCT
TTATTTAATATATATGATGAAAATGGTAAAAAGGTTAATAGAAATGATGGTGCTATTAATGAAAAAGGAAATGTAATGGG
AACATATATTCATGGAGTATTTGATGGAGTAGAGTTCAGAGAAAAAGTATTAAATAATATAAGAAAATCTAAAAATATGG
AAGAAAGAAATGCAATAAATTATGAAAATCTAAGAGAAAAGAATTTAGATATGCTAGCTGATTTAGTTAGAGAAAATATA
GATATGGATTACATATATAAAATAATTGGCATGTAA

Upstream 100 bases:

>100_bases
TTCTAAAAGATCTAAAGAATGAATTGTCAACCTCCTAGAAGACAACCTTCTATCAAACTCAAGAACACAGCTAATTTTTT
TACTAAAGATTAATAGAGAG

Downstream 100 bases:

>100_bases
GAAAGAGGTGGTTTCCATTAAAACTCCAATTTTAATGGGATTAATTGATATAATAGTTGCAGTTTTATTAGACTTTGCAA
TAGGCGACCCGTATTGGTTT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRAQVLQAHAAGLEPEAYMNPIL
LKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQFKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDA
PVLLAGDIDRGGVFASLAGTMLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAI
DFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNTIEDLLALRKCGLEEKIKEYS
KTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNVDTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKP
LFNIYDENGKKVNRNDGAINEKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENI
DMDYIYKIIGM

Sequences:

>Translated_491_residues
MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRAQVLQAHAAGLEPEAYMNPIL
LKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQFKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDA
PVLLAGDIDRGGVFASLAGTMLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAI
DFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNTIEDLLALRKCGLEEKIKEYS
KTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNVDTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKP
LFNIYDENGKKVNRNDGAINEKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENI
DMDYIYKIIGM
>Mature_491_residues
MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRAQVLQAHAAGLEPEAYMNPIL
LKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQFKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDA
PVLLAGDIDRGGVFASLAGTMLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAI
DFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNTIEDLLALRKCGLEEKIKEYS
KTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNVDTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKP
LFNIYDENGKKVNRNDGAINEKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENI
DMDYIYKIIGM

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CLOTE (Q897L4)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781385.1
- ProteinModelPortal:   Q897L4
- GeneID:   1060812
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00720
- NMPDR:   fig|212717.1.peg.607
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   CTET212717:CTC_00720-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 55119; Mature: 55119

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 329-329 ACT_SITE 431-431

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCHHHCHH
QVLQAHAAGLEPEAYMNPILLKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQ
HHHHHHHCCCCCHHHCCCEEEECCCCCEEEEEECCEEECCCCEEHHHHHHHHHHHHHHHH
FKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDAPVLLAGDIDRGGVFASLAGT
HHCHHHHEEEEEEECCCCCCEEEECCCHHHHCCHHHHCCCCEEEECCCCCCCCHHHHHCE
MLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAI
EEEEEECHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCE
DFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNT
EECCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEECCCCCH
IEDLLALRKCGLEEKIKEYSKTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNV
HHHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCEEEH
DTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKPLFNIYDENGKKVNRNDGAIN
HHHHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCHHHEEECCCCCEECCCCCCCC
EKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENI
CCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCHHHHHHHHHHCC
DMDYIYKIIGM
CHHHHHHHHCC
>Mature Secondary Structure
MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCHHHCHH
QVLQAHAAGLEPEAYMNPILLKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQ
HHHHHHHCCCCCHHHCCCEEEECCCCCEEEEEECCEEECCCCEEHHHHHHHHHHHHHHHH
FKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDAPVLLAGDIDRGGVFASLAGT
HHCHHHHEEEEEEECCCCCCEEEECCCHHHHCCHHHHCCCCEEEECCCCCCCCHHHHHCE
MLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAI
EEEEEECHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCE
DFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNT
EECCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEECCCCCH
IEDLLALRKCGLEEKIKEYSKTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNV
HHHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCEEEH
DTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKPLFNIYDENGKKVNRNDGAIN
HHHHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCHHHEEECCCCCEECCCCCCCC
EKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENI
CCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCHHHHHHHHHHCC
DMDYIYKIIGM
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129