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The map label for this gene is ribA [H]

Identifier: 28210397

GI number: 28210397

Start: 690710

End: 691918

Strand: Direct

Name: ribA [H]

Synonym: CTC00673

Alternate gene names: 28210397

Gene position: 690710-691918 (Clockwise)

Preceding gene: 28210396

Following gene: 28210398

Centisome position: 24.67

GC content: 32.67

Gene sequence:

>1209_bases
GTGGTAAAAATGTTTAAGTTTAATAGTATTGAAGAGGCTATTGCTGATATAAAAGAAGGAAAAATGGTTATTGTGGTAGA
TGATGAGGATAGGGAAAATGAGGGGGATCTTTTAATGGCAGCAGAAAAAGTAACTCCTGAAAATATTAATTTTATGATTA
AATATGGAAGAGGATTAGTGTGCATGCCTATAATTGGAGAAAGATTAAAGGAATTAAACCTTAATCAAATGGTAGATATA
AATACAGATACAAATGGAACAGCTTTTACAGTCTCCATAGACTTCATAGATACTACTACAGGTATTTCAGCTTATGAAAG
GGCTCATACCATATCAAAAGTATTAGATAGCAGTGTAAAGGGAGAGGACTTTAAAAGACCAGGACATGTTTTTCCATTAG
AAGCAAAAGAAGGTGGGGTATTAAAAAGAGCAGGACATACTGAAGCTTCTGTAGATCTAGCAAGGCTTGCAGGATTTTAT
CCAGCAGGAGTAATTTGTGAGATTGTAGGGGAAGATGGAAAGATGGCAAGACTCCCTCAATTAATGGAGTATTCTAAGGA
ACATAATTTAAAAATTATAAATATAGCAGATTTAATAGCTTATAGAAGAAAGAAAGAAACTCTAGTAAAAAGAGTTGTAG
AAGCTAAAATGCCAACAAGATGGGGAGAATTTAAAATAATAGGCTATGAAAACAAAATAAATGGTGAGCACCATGTAGCC
CTTGTAATGGGAGATATAGAAAATGGAGAAGATGTATTAGTTAGAATGCATTCAGAATGTCTTACAGGAGATGCTCTAGG
CTCTGTAAGATGTGATTGTGGGTATCAATATGAAGCAGCTATGAAAGCTATAAGTGAAGAGAGAAGAGGAGTACTAGTAT
ACATGCGCCAAGAAGGAAGAGGTATAGGGCTTATTAACAAATTAAAGGCTTATAATCTTCAAGACAAGGGCATGGATACT
GTGGAAGCTAATATTGCCTTAGGTTTTCCACCTGATTTAAGAGATTATGGAATAGGAGCTCAAATATTAAATGATTTAGG
AATTAAAAAGATAAATCTTATGACAAACAATCCTAAAAAAATAACTTCATTATCTGGTTATGGAATAAAAATAGTAAAAA
GAGTTCCACTAGAAATTCATGAAAATGAGGAAAGTGAATTTTACCTAAAAACTAAAAAAGAAAAAATGGGTCATTTATTA
CATTTTTAA

Upstream 100 bases:

>100_bases
ATATATAAATCATACAATTAAAATTTTTTTGTAATGATAATGGAGAAAAAATAACCACAATTGTTCATTCTTCATTGTTC
ATTATTCATTAAATAAAGGG

Downstream 100 bases:

>100_bases
TATATAAAATTGGGAGGAATATAAAATGAATATAATAGAAGGAAAATTAATAGGACAGAGTTTGAAGTTTGGTATAACTA
TAGGAAGATTTAATGAATTT

Product: riboflavin biosynthesis protein ribA

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MVKMFKFNSIEEAIADIKEGKMVIVVDDEDRENEGDLLMAAEKVTPENINFMIKYGRGLVCMPIIGERLKELNLNQMVDI
NTDTNGTAFTVSIDFIDTTTGISAYERAHTISKVLDSSVKGEDFKRPGHVFPLEAKEGGVLKRAGHTEASVDLARLAGFY
PAGVICEIVGEDGKMARLPQLMEYSKEHNLKIINIADLIAYRRKKETLVKRVVEAKMPTRWGEFKIIGYENKINGEHHVA
LVMGDIENGEDVLVRMHSECLTGDALGSVRCDCGYQYEAAMKAISEERRGVLVYMRQEGRGIGLINKLKAYNLQDKGMDT
VEANIALGFPPDLRDYGIGAQILNDLGIKKINLMTNNPKKITSLSGYGIKIVKRVPLEIHENEESEFYLKTKKEKMGHLL
HF

Sequences:

>Translated_402_residues
MVKMFKFNSIEEAIADIKEGKMVIVVDDEDRENEGDLLMAAEKVTPENINFMIKYGRGLVCMPIIGERLKELNLNQMVDI
NTDTNGTAFTVSIDFIDTTTGISAYERAHTISKVLDSSVKGEDFKRPGHVFPLEAKEGGVLKRAGHTEASVDLARLAGFY
PAGVICEIVGEDGKMARLPQLMEYSKEHNLKIINIADLIAYRRKKETLVKRVVEAKMPTRWGEFKIIGYENKINGEHHVA
LVMGDIENGEDVLVRMHSECLTGDALGSVRCDCGYQYEAAMKAISEERRGVLVYMRQEGRGIGLINKLKAYNLQDKGMDT
VEANIALGFPPDLRDYGIGAQILNDLGIKKINLMTNNPKKITSLSGYGIKIVKRVPLEIHENEESEFYLKTKKEKMGHLL
HF
>Mature_402_residues
MVKMFKFNSIEEAIADIKEGKMVIVVDDEDRENEGDLLMAAEKVTPENINFMIKYGRGLVCMPIIGERLKELNLNQMVDI
NTDTNGTAFTVSIDFIDTTTGISAYERAHTISKVLDSSVKGEDFKRPGHVFPLEAKEGGVLKRAGHTEASVDLARLAGFY
PAGVICEIVGEDGKMARLPQLMEYSKEHNLKIINIADLIAYRRKKETLVKRVVEAKMPTRWGEFKIIGYENKINGEHHVA
LVMGDIENGEDVLVRMHSECLTGDALGSVRCDCGYQYEAAMKAISEERRGVLVYMRQEGRGIGLINKLKAYNLQDKGMDT
VEANIALGFPPDLRDYGIGAQILNDLGIKKINLMTNNPKKITSLSGYGIKIVKRVPLEIHENEESEFYLKTKKEKMGHLL
HF

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1787533, Length=192, Percent_Identity=58.3333333333333, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=48.7562189054726, Blast_Score=213, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=42.0289855072464, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319438, Length=171, Percent_Identity=49.7076023391813, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 45030; Mature: 45030

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMFKFNSIEEAIADIKEGKMVIVVDDEDRENEGDLLMAAEKVTPENINFMIKYGRGLV
CEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEE
CMPIIGERLKELNLNQMVDINTDTNGTAFTVSIDFIDTTTGISAYERAHTISKVLDSSVK
EHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GEDFKRPGHVFPLEAKEGGVLKRAGHTEASVDLARLAGFYPAGVICEIVGEDGKMARLPQ
CCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
LMEYSKEHNLKIINIADLIAYRRKKETLVKRVVEAKMPTRWGEFKIIGYENKINGEHHVA
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEE
LVMGDIENGEDVLVRMHSECLTGDALGSVRCDCGYQYEAAMKAISEERRGVLVYMRQEGR
EEEECCCCCCCEEEEEHHHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCC
GIGLINKLKAYNLQDKGMDTVEANIALGFPPDLRDYGIGAQILNDLGIKKINLMTNNPKK
CCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHCCCEEEEEEECCCCC
ITSLSGYGIKIVKRVPLEIHENEESEFYLKTKKEKMGHLLHF
EEECCCCCEEEEEECCEEEECCCCCEEEEEECHHHHHHHCCC
>Mature Secondary Structure
MVKMFKFNSIEEAIADIKEGKMVIVVDDEDRENEGDLLMAAEKVTPENINFMIKYGRGLV
CEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEE
CMPIIGERLKELNLNQMVDINTDTNGTAFTVSIDFIDTTTGISAYERAHTISKVLDSSVK
EHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GEDFKRPGHVFPLEAKEGGVLKRAGHTEASVDLARLAGFYPAGVICEIVGEDGKMARLPQ
CCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
LMEYSKEHNLKIINIADLIAYRRKKETLVKRVVEAKMPTRWGEFKIIGYENKINGEHHVA
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEE
LVMGDIENGEDVLVRMHSECLTGDALGSVRCDCGYQYEAAMKAISEERRGVLVYMRQEGR
EEEECCCCCCCEEEEEHHHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCC
GIGLINKLKAYNLQDKGMDTVEANIALGFPPDLRDYGIGAQILNDLGIKKINLMTNNPKK
CCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHCCCEEEEEEECCCCC
ITSLSGYGIKIVKRVPLEIHENEESEFYLKTKKEKMGHLLHF
EEECCCCCEEEEEECCEEEECCCCCEEEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA