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The map label for this gene is ribE [H]

Identifier: 28210396

GI number: 28210396

Start: 689707

End: 690357

Strand: Direct

Name: ribE [H]

Synonym: CTC00672

Alternate gene names: 28210396

Gene position: 689707-690357 (Clockwise)

Preceding gene: 28210395

Following gene: 28210397

Centisome position: 24.64

GC content: 31.64

Gene sequence:

>651_bases
TTGTTTACGGGTATAGTAGAGGAATTAGGAAGTATAGAGAATATAGAAAAAAAGGGAAACGCCTATAGCATAAAAATAAA
GGCTAAAAAGGTTTTGGAGGATGTAAATCTAGGAGATAGTATATGCACTAATGGAGTTTGTCTTACAGTTACTAACTTTT
CAAAGGATAGTTTTACTGTGGATGTAATGCCAGAAACTATAAGACAAAGTAATCTAAAAAATATTAAAAAAGGCAGTTTA
GTAAATTTAGAAAGGGCTCTAAAGGCTACAGGAAGATTAGGTGGACATATAGTTAGCGGACACATAGATGGAGAAGGGAT
TATAAAGGAATATAAAAAAGAAGGAAATGCTTGGTGGATTTCAGTAGAACCTGAAAAAGGACTTCTAAAGTATGTAATAG
AAAGAGGTTCTATAGCCTTAGATGGTGTAAGCCTAACCGTAGCTTATGTAGATGAAAAGCTATTTAAAGTTTCTGTAATC
CCTAATACCAGTGAAAAAACTACCCTATTAAAAAAAGGAGTAGGGGACATTTTAAACATAGAATGCGATTTAATAGGAAA
ATATGTAGAGAAAATCTTAAATTTCAAAAATCATAAAGAAGAGCAAAGTAAAATAGACATGGACTTTTTGAAAAACAACG
GATTTGTTTAG

Upstream 100 bases:

>100_bases
GATTGCATAGAACTAAAGGATTTATCTATTAAAAATTTTAAAGAAGATATTTTGATAGAAGGCTTTATTTAGATAATAAC
TAATAAAAGGTGGTGTTTTA

Downstream 100 bases:

>100_bases
CTAATAACTAATAGTGAATAGTGAATAACTAAGGAGGATTTTCTTCCGCTTTGCTACAGAAAATCTTTAATTTAAAGGTA
GTTATACTATGGAAAACTCC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFTGIVEELGSIENIEKKGNAYSIKIKAKKVLEDVNLGDSICTNGVCLTVTNFSKDSFTVDVMPETIRQSNLKNIKKGSL
VNLERALKATGRLGGHIVSGHIDGEGIIKEYKKEGNAWWISVEPEKGLLKYVIERGSIALDGVSLTVAYVDEKLFKVSVI
PNTSEKTTLLKKGVGDILNIECDLIGKYVEKILNFKNHKEEQSKIDMDFLKNNGFV

Sequences:

>Translated_216_residues
MFTGIVEELGSIENIEKKGNAYSIKIKAKKVLEDVNLGDSICTNGVCLTVTNFSKDSFTVDVMPETIRQSNLKNIKKGSL
VNLERALKATGRLGGHIVSGHIDGEGIIKEYKKEGNAWWISVEPEKGLLKYVIERGSIALDGVSLTVAYVDEKLFKVSVI
PNTSEKTTLLKKGVGDILNIECDLIGKYVEKILNFKNHKEEQSKIDMDFLKNNGFV
>Mature_216_residues
MFTGIVEELGSIENIEKKGNAYSIKIKAKKVLEDVNLGDSICTNGVCLTVTNFSKDSFTVDVMPETIRQSNLKNIKKGSL
VNLERALKATGRLGGHIVSGHIDGEGIIKEYKKEGNAWWISVEPEKGLLKYVIERGSIALDGVSLTVAYVDEKLFKVSVI
PNTSEKTTLLKKGVGDILNIECDLIGKYVEKILNFKNHKEEQSKIDMDFLKNNGFV

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=210, Percent_Identity=37.1428571428571, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319733, Length=212, Percent_Identity=41.5094339622642, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23908; Mature: 23908

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEELGSIENIEKKGNAYSIKIKAKKVLEDVNLGDSICTNGVCLTVTNFSKDSFTV
CCCCHHHHHCCHHHHHHCCCEEEEEEEHHHHHHHCCCCCHHHCCCEEEEEEECCCCCEEE
DVMPETIRQSNLKNIKKGSLVNLERALKATGRLGGHIVSGHIDGEGIIKEYKKEGNAWWI
EECHHHHHHHHHHHCCCCCEECHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
SVEPEKGLLKYVIERGSIALDGVSLTVAYVDEKLFKVSVIPNTSEKTTLLKKGVGDILNI
EECCCCHHHHHHHHCCCEEECCCEEEEEEECCEEEEEEECCCCCHHHHHHHHCCCCEEEE
ECDLIGKYVEKILNFKNHKEEQSKIDMDFLKNNGFV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFTGIVEELGSIENIEKKGNAYSIKIKAKKVLEDVNLGDSICTNGVCLTVTNFSKDSFTV
CCCCHHHHHCCHHHHHHCCCEEEEEEEHHHHHHHCCCCCHHHCCCEEEEEEECCCCCEEE
DVMPETIRQSNLKNIKKGSLVNLERALKATGRLGGHIVSGHIDGEGIIKEYKKEGNAWWI
EECHHHHHHHHHHHCCCCCEECHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
SVEPEKGLLKYVIERGSIALDGVSLTVAYVDEKLFKVSVIPNTSEKTTLLKKGVGDILNI
EECCCCHHHHHHHHCCCEEECCCEEEEEEECCEEEEEEECCCCCHHHHHHHHCCCCEEEE
ECDLIGKYVEKILNFKNHKEEQSKIDMDFLKNNGFV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]