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The map label for this gene is fliY [H]

Identifier: 28210295

GI number: 28210295

Start: 576911

End: 577729

Strand: Direct

Name: fliY [H]

Synonym: CTC00557

Alternate gene names: 28210295

Gene position: 576911-577729 (Clockwise)

Preceding gene: 28210293

Following gene: 28210296

Centisome position: 20.61

GC content: 28.21

Gene sequence:

>819_bases
ATGAAAAAGACACTTTCTATATTGGTGGTATTTTTAGTATCAATGCTACTCATTATAGGTTGCGGTAATAAAAATACAAG
TAATAAAGGAAATGATAAAAATAATAAAAGTTTATCTACACTTGAAAAGATAAAAAAAGATGGGGTATTAAAAATGGGAC
TTAATGATACATATCCACCTATGGAATTTAGAGATGAAAGTCATAATCTTGTTGGATTTGATGTGGATTTAGGGAAAGAA
ATTGCTAAAAAATTAGGAGTGAAATTAGAAATTATAACTAATGATTGGTCAGGAATAGTTCTATCATTAAAATCTAAGAA
ATACGATATGATACTTTCCACTATGAGCATAACAGAAGAAAGAAAAAAAGAAATGAATTTTTCTGAACCTTATATAGTTG
GAGGGCAAAAATTAGTAGTTAAAAAAGATGAAAAAGATATAAAGAAGGTAGAAGATTTAAAAGGTAAAGTAATAGGATGT
CAAATGGGGACAACAGGAGAGGTTTCTGCAAATAAAATTGAAGGAATTAAAGAACTTAAAAAATATGATGGGGTAACACA
AGCTTTTAATGATTTATCTGTGGGAAGGATAAATGCAGTAATTGCAGATGGACAAGTTGCAGGCTATTATCTTAAAAAAC
GTAGTGGAGATTTAGTTCTATTAGATACTAAATTAACTAGTGAACCTGTAGGAATAGGATTTAGAAAAGAAGATACAGAT
ATAAGAGATGAAGTTCAAAAAACTTTAGATGAGTTAAAGAAAGAAGGTACTCTTTCTAAACTATCAGAAAAGTGGTTTGG
ATATGATATATATAAATAA

Upstream 100 bases:

>100_bases
AATCCATAGGAAACTTTTTAAAAAGTGTTGCATAAATATAAATAAAGATGTATAATCATAATCATTGAAGGGGGCAAATT
AAATAAAAGGAGTTGGGGCT

Downstream 100 bases:

>100_bases
CAATAAAAAGGGGAGAAAAGAACAATGAAAAAAATATTAGCGTTATTATTAAGTACTTTAGTAGTAGGTTCAATTTTTGT
TGGATGCAGCAAAAATGACA

Product: cystine/glutamine-binding periplasmic protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKTLSILVVFLVSMLLIIGCGNKNTSNKGNDKNNKSLSTLEKIKKDGVLKMGLNDTYPPMEFRDESHNLVGFDVDLGKE
IAKKLGVKLEIITNDWSGIVLSLKSKKYDMILSTMSITEERKKEMNFSEPYIVGGQKLVVKKDEKDIKKVEDLKGKVIGC
QMGTTGEVSANKIEGIKELKKYDGVTQAFNDLSVGRINAVIADGQVAGYYLKKRSGDLVLLDTKLTSEPVGIGFRKEDTD
IRDEVQKTLDELKKEGTLSKLSEKWFGYDIYK

Sequences:

>Translated_272_residues
MKKTLSILVVFLVSMLLIIGCGNKNTSNKGNDKNNKSLSTLEKIKKDGVLKMGLNDTYPPMEFRDESHNLVGFDVDLGKE
IAKKLGVKLEIITNDWSGIVLSLKSKKYDMILSTMSITEERKKEMNFSEPYIVGGQKLVVKKDEKDIKKVEDLKGKVIGC
QMGTTGEVSANKIEGIKELKKYDGVTQAFNDLSVGRINAVIADGQVAGYYLKKRSGDLVLLDTKLTSEPVGIGFRKEDTD
IRDEVQKTLDELKKEGTLSKLSEKWFGYDIYK
>Mature_272_residues
MKKTLSILVVFLVSMLLIIGCGNKNTSNKGNDKNNKSLSTLEKIKKDGVLKMGLNDTYPPMEFRDESHNLVGFDVDLGKE
IAKKLGVKLEIITNDWSGIVLSLKSKKYDMILSTMSITEERKKEMNFSEPYIVGGQKLVVKKDEKDIKKVEDLKGKVIGC
QMGTTGEVSANKIEGIKELKKYDGVTQAFNDLSVGRINAVIADGQVAGYYLKKRSGDLVLLDTKLTSEPVGIGFRKEDTD
IRDEVQKTLDELKKEGTLSKLSEKWFGYDIYK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=34.9809885931559, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=35.4978354978355, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788649, Length=232, Percent_Identity=31.8965517241379, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787031, Length=219, Percent_Identity=33.7899543378995, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=32.1266968325792, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787088, Length=223, Percent_Identity=30.0448430493274, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786876, Length=245, Percent_Identity=28.1632653061224, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30387; Mature: 30387

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLSILVVFLVSMLLIIGCGNKNTSNKGNDKNNKSLSTLEKIKKDGVLKMGLNDTYPP
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCC
MEFRDESHNLVGFDVDLGKEIAKKLGVKLEIITNDWSGIVLSLKSKKYDMILSTMSITEE
CCCCCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
RKKEMNFSEPYIVGGQKLVVKKDEKDIKKVEDLKGKVIGCQMGTTGEVSANKIEGIKELK
HHHHCCCCCCEEECCCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
KYDGVTQAFNDLSVGRINAVIADGQVAGYYLKKRSGDLVLLDTKLTSEPVGIGFRKEDTD
HHCCHHHHHHCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCC
IRDEVQKTLDELKKEGTLSKLSEKWFGYDIYK
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKTLSILVVFLVSMLLIIGCGNKNTSNKGNDKNNKSLSTLEKIKKDGVLKMGLNDTYPP
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCC
MEFRDESHNLVGFDVDLGKEIAKKLGVKLEIITNDWSGIVLSLKSKKYDMILSTMSITEE
CCCCCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
RKKEMNFSEPYIVGGQKLVVKKDEKDIKKVEDLKGKVIGCQMGTTGEVSANKIEGIKELK
HHHHCCCCCCEEECCCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
KYDGVTQAFNDLSVGRINAVIADGQVAGYYLKKRSGDLVLLDTKLTSEPVGIGFRKEDTD
HHCCHHHHHHCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCC
IRDEVQKTLDELKKEGTLSKLSEKWFGYDIYK
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]