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The map label for this gene is fliY [H]

Identifier: 28210296

GI number: 28210296

Start: 577754

End: 578569

Strand: Direct

Name: fliY [H]

Synonym: CTC00558

Alternate gene names: 28210296

Gene position: 577754-578569 (Clockwise)

Preceding gene: 28210295

Following gene: 28210297

Centisome position: 20.64

GC content: 29.17

Gene sequence:

>816_bases
ATGAAAAAAATATTAGCGTTATTATTAAGTACTTTAGTAGTAGGTTCAATTTTTGTTGGATGCAGCAAAAATGACAATAA
AGAAAAGGACGATAAAAAGAATGTAAATGTGTTACAAAAGATAAAGGATAATAAAGTAATAAAAGTTGGTCTTGAGGATA
CTTATCCTCCAATGGAATTTAGAAACGAAAAAAATGAACTCATAGGATTTGATATTGATTTATGTGATGAAATAGGTAAG
AGACTAGGGGGAAAAGTAGAATATGTAATGACTGAATTTGGTGGTCTTATGATGTCATTGAATTCAAATAAAATAGATAT
GTCAGCTTCAGCAATAAGTATAACAGAGGAAAGAAAAAAAGAAGCAGAATTTACAAAACCCTATGTAGATAGTGGTCAAG
CGTTAGTAGTTAAAAAGGGAAATACAAGTATAAAAGATGAAAAAGATTTAAAAGGTAAAATAGTAGGGGCACAACTTGGA
ACCACAGGAGAAATAGCAGCTAAGAAAATAGAAGGTGTTAAAGAAGTAAAGGCTTATGATAAGGTTCCTCAAGTGTTTCA
AGACCTTGAAATAGGAAGAATAGATGCAGTTATAGTAGATGAATTTGTTGGAAGATATTATTTATCAAAACAAAAAGATA
AATCAGAGGTAATAAAATCCCTAGAACAAGAACCAATAGGAATAGCTTTTAAAAAGGGAGAAAAAGAATTACAAGAAGAA
GTTCAAAAAATTATAGATGAAATGATTAAAGATGGAACTATGTCTAAGATATCAGAAAAATGGTTTGGAGCAGACATATA
TAAAGATCAAAAATAA

Upstream 100 bases:

>100_bases
TTTAGATGAGTTAAAGAAAGAAGGTACTCTTTCTAAACTATCAGAAAAGTGGTTTGGATATGATATATATAAATAACAAT
AAAAAGGGGAGAAAAGAACA

Downstream 100 bases:

>100_bases
AAATTAGAATAGGGCACTAATTTATTAGTGCCTGATAATTTTTCAAGTTATAAAAAGGGGGACGATTATAATGGATATGA
ATTTACTTAAGAATATAATT

Product: cystine/glutamine-binding periplasmic protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKILALLLSTLVVGSIFVGCSKNDNKEKDDKKNVNVLQKIKDNKVIKVGLEDTYPPMEFRNEKNELIGFDIDLCDEIGK
RLGGKVEYVMTEFGGLMMSLNSNKIDMSASAISITEERKKEAEFTKPYVDSGQALVVKKGNTSIKDEKDLKGKIVGAQLG
TTGEIAAKKIEGVKEVKAYDKVPQVFQDLEIGRIDAVIVDEFVGRYYLSKQKDKSEVIKSLEQEPIGIAFKKGEKELQEE
VQKIIDEMIKDGTMSKISEKWFGADIYKDQK

Sequences:

>Translated_271_residues
MKKILALLLSTLVVGSIFVGCSKNDNKEKDDKKNVNVLQKIKDNKVIKVGLEDTYPPMEFRNEKNELIGFDIDLCDEIGK
RLGGKVEYVMTEFGGLMMSLNSNKIDMSASAISITEERKKEAEFTKPYVDSGQALVVKKGNTSIKDEKDLKGKIVGAQLG
TTGEIAAKKIEGVKEVKAYDKVPQVFQDLEIGRIDAVIVDEFVGRYYLSKQKDKSEVIKSLEQEPIGIAFKKGEKELQEE
VQKIIDEMIKDGTMSKISEKWFGADIYKDQK
>Mature_271_residues
MKKILALLLSTLVVGSIFVGCSKNDNKEKDDKKNVNVLQKIKDNKVIKVGLEDTYPPMEFRNEKNELIGFDIDLCDEIGK
RLGGKVEYVMTEFGGLMMSLNSNKIDMSASAISITEERKKEAEFTKPYVDSGQALVVKKGNTSIKDEKDLKGKIVGAQLG
TTGEIAAKKIEGVKEVKAYDKVPQVFQDLEIGRIDAVIVDEFVGRYYLSKQKDKSEVIKSLEQEPIGIAFKKGEKELQEE
VQKIIDEMIKDGTMSKISEKWFGADIYKDQK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=40, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=35.4978354978355, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787031, Length=224, Percent_Identity=31.6964285714286, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=32.0512820512821, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787088, Length=224, Percent_Identity=32.5892857142857, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786876, Length=242, Percent_Identity=26.8595041322314, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30387; Mature: 30387

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILALLLSTLVVGSIFVGCSKNDNKEKDDKKNVNVLQKIKDNKVIKVGLEDTYPPMEF
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHH
RNEKNELIGFDIDLCDEIGKRLGGKVEYVMTEFGGLMMSLNSNKIDMSASAISITEERKK
CCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCEECCHHHHHHHHHHHH
EAEFTKPYVDSGQALVVKKGNTSIKDEKDLKGKIVGAQLGTTGEIAAKKIEGVKEVKAYD
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KVPQVFQDLEIGRIDAVIVDEFVGRYYLSKQKDKSEVIKSLEQEPIGIAFKKGEKELQEE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCHHHHHHH
VQKIIDEMIKDGTMSKISEKWFGADIYKDQK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKILALLLSTLVVGSIFVGCSKNDNKEKDDKKNVNVLQKIKDNKVIKVGLEDTYPPMEF
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHH
RNEKNELIGFDIDLCDEIGKRLGGKVEYVMTEFGGLMMSLNSNKIDMSASAISITEERKK
CCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCEECCHHHHHHHHHHHH
EAEFTKPYVDSGQALVVKKGNTSIKDEKDLKGKIVGAQLGTTGEIAAKKIEGVKEVKAYD
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KVPQVFQDLEIGRIDAVIVDEFVGRYYLSKQKDKSEVIKSLEQEPIGIAFKKGEKELQEE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCHHHHHHH
VQKIIDEMIKDGTMSKISEKWFGADIYKDQK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]