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The map label for this gene is lytC [H]

Identifier: 28210260

GI number: 28210260

Start: 527266

End: 529023

Strand: Direct

Name: lytC [H]

Synonym: CTC00518

Alternate gene names: 28210260

Gene position: 527266-529023 (Clockwise)

Preceding gene: 28210257

Following gene: 28210261

Centisome position: 18.84

GC content: 29.01

Gene sequence:

>1758_bases
ATGTTTAAAAAAGGTAAAAAATTTTTATCTATTTGTATTACCACAATGCTAAGTTTAACAACTGCTATAACAACTACAAA
AACATCAGCAGCGGAATTGGCGAAAATTACAAGAATCTGTGGAGTAGATAGATACCAAACAGCTGTAAAAATATCGCAAA
ATGGGTGGAAATCAGGATCAGATTACGCAATACTAGCATCAGGAGAAGATTATGCTGATGCTCTATGTGCAACTTCACTA
GCAAAAGCTAAAGATGCTCCTATATTCCTTACAAAGAAGAATTCATTAAATGAAGAAACTTTAAAAGAACTAAAAAGGTT
AGGAGTAAAAAAGCTTTATATCATAGGTGGATATGGTTCTATATCAGAGGAAATAGAAAATAAAGTTAAATCAGTAGTAA
AAAATACTGAAAGACTACAAGGAAAAGACAGATATGAGACTTCAGTTAAAATTGCAGAAAAATTAGGGGAAAGTAATAAA
GTTATATTAACATCAGGAGAAGGCTATGCAGATGCTTTATCAATAGCACCAGTTGCAGCAATAAAAGGAATTCCAGTAGT
TTTAACAAAGGTTAGAGAACTACCAACATCTACAAAAGAATATATAAAATCCATAGGTGCAACAACAACTTATGTAATAG
GTGGAACTGAAGCTATTAGTGATACAGTAAAAAATTTAGTACCAAGTCCAGAAAGAATATATGGACAAGATAGATTTGAA
ACTAATTCAGAAATTATAAAAGCTTTTGCCATAGAATTTGATTTTGAAAACGCATACGTGGCATTAGGGGTAGGTTCTAC
AGGAAAAGAATTTGCAGATGGTCTATCAGCTTCAGCCCTATCAGCTAAAAATAGAGCACCAGTAATTTTAACTGGACAAA
CCTTAAATAGCGTAACAAGAGATTTAGCAAGAGAAAAACTTTATCCAACAACTAATTTAACAGTTTTAGGTGGAGTTAAA
AATATTTCAGATACAGCTGTAGGTGAAATGAAAGTAACAGGTGAAATTATAAAAACTGAAAAGGAGCTTGAAGATAAAAA
TATAGATGGAAACTTAGCAATTACAGTTAAGAATGCAGAGTTAAGAGATAGCAAAATAAAGGGAAATTTATATATTGAAA
ATGATAATATATTATTATCAAATGTAAAAGTAGATGGAACTATTTATATTAATCCTGGAAGTGAAAATACATGTAAATTA
GAAAATGTAATGGCTAATAAAATAATAGTATTATCAGGTAGAGAAGAAGGAATAAATTTAATATCAACTAAAGCAGAAAA
ATTAGATATATTAAATAGGGATAATGCAAGAGTAATTTTAGAACAAGACACAGATATATCAGAAACAAAATCTTTATCTA
GTGCAATACTTCAAGTAGAAGATGGATGTTTTGGAGAAGTTATAATACCAGAAACACTTAATGAAAAGACAATACAATTT
AAAGGGAATTTTGATAAAACAATAAAAGTAGAAGGACAAGCATATTTAAAGACATACTCTAATGCTGTTATTAAAAATGT
TGAAATAAGATCAGATAAAAATAATGAAGTCGTATTATATGGAAGATTTAATGATGTAGATGTATATAGTAATGCAGATA
TAAAAACTAAGGAAGATACAAGTGCTATAATAAGGGCGAAAAATTCTGAAGCAAAATTAAATGCTAAAATATATGTACCT
GAAGATACAGATATAAAAGTTAGAGAGTTTAAAGAAAAGAATATATCAGGATATGGGAGAAAGAAAGCATTATTTTAA

Upstream 100 bases:

>100_bases
ATGTATTAAAATAGTATTTATAGAATGTAAAGTAAAATTTAAGATATATATGTAAAAATGTATTTATATTTTTAATTGAG
ATGAGGGGGAGGAATTTAAG

Downstream 100 bases:

>100_bases
TTAAAAGATAGATAGTGAAATTATAACCATATATTGACAAAACACACGGGAAACGCTAAAATTAAATTGTGTATTAAGAA
TAGTCAGAAAAATGTAAAAA

Product: cwp66 homolog/N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MFKKGKKFLSICITTMLSLTTAITTTKTSAAELAKITRICGVDRYQTAVKISQNGWKSGSDYAILASGEDYADALCATSL
AKAKDAPIFLTKKNSLNEETLKELKRLGVKKLYIIGGYGSISEEIENKVKSVVKNTERLQGKDRYETSVKIAEKLGESNK
VILTSGEGYADALSIAPVAAIKGIPVVLTKVRELPTSTKEYIKSIGATTTYVIGGTEAISDTVKNLVPSPERIYGQDRFE
TNSEIIKAFAIEFDFENAYVALGVGSTGKEFADGLSASALSAKNRAPVILTGQTLNSVTRDLAREKLYPTTNLTVLGGVK
NISDTAVGEMKVTGEIIKTEKELEDKNIDGNLAITVKNAELRDSKIKGNLYIENDNILLSNVKVDGTIYINPGSENTCKL
ENVMANKIIVLSGREEGINLISTKAEKLDILNRDNARVILEQDTDISETKSLSSAILQVEDGCFGEVIIPETLNEKTIQF
KGNFDKTIKVEGQAYLKTYSNAVIKNVEIRSDKNNEVVLYGRFNDVDVYSNADIKTKEDTSAIIRAKNSEAKLNAKIYVP
EDTDIKVREFKEKNISGYGRKKALF

Sequences:

>Translated_585_residues
MFKKGKKFLSICITTMLSLTTAITTTKTSAAELAKITRICGVDRYQTAVKISQNGWKSGSDYAILASGEDYADALCATSL
AKAKDAPIFLTKKNSLNEETLKELKRLGVKKLYIIGGYGSISEEIENKVKSVVKNTERLQGKDRYETSVKIAEKLGESNK
VILTSGEGYADALSIAPVAAIKGIPVVLTKVRELPTSTKEYIKSIGATTTYVIGGTEAISDTVKNLVPSPERIYGQDRFE
TNSEIIKAFAIEFDFENAYVALGVGSTGKEFADGLSASALSAKNRAPVILTGQTLNSVTRDLAREKLYPTTNLTVLGGVK
NISDTAVGEMKVTGEIIKTEKELEDKNIDGNLAITVKNAELRDSKIKGNLYIENDNILLSNVKVDGTIYINPGSENTCKL
ENVMANKIIVLSGREEGINLISTKAEKLDILNRDNARVILEQDTDISETKSLSSAILQVEDGCFGEVIIPETLNEKTIQF
KGNFDKTIKVEGQAYLKTYSNAVIKNVEIRSDKNNEVVLYGRFNDVDVYSNADIKTKEDTSAIIRAKNSEAKLNAKIYVP
EDTDIKVREFKEKNISGYGRKKALF
>Mature_585_residues
MFKKGKKFLSICITTMLSLTTAITTTKTSAAELAKITRICGVDRYQTAVKISQNGWKSGSDYAILASGEDYADALCATSL
AKAKDAPIFLTKKNSLNEETLKELKRLGVKKLYIIGGYGSISEEIENKVKSVVKNTERLQGKDRYETSVKIAEKLGESNK
VILTSGEGYADALSIAPVAAIKGIPVVLTKVRELPTSTKEYIKSIGATTTYVIGGTEAISDTVKNLVPSPERIYGQDRFE
TNSEIIKAFAIEFDFENAYVALGVGSTGKEFADGLSASALSAKNRAPVILTGQTLNSVTRDLAREKLYPTTNLTVLGGVK
NISDTAVGEMKVTGEIIKTEKELEDKNIDGNLAITVKNAELRDSKIKGNLYIENDNILLSNVKVDGTIYINPGSENTCKL
ENVMANKIIVLSGREEGINLISTKAEKLDILNRDNARVILEQDTDISETKSLSSAILQVEDGCFGEVIIPETLNEKTIQF
KGNFDKTIKVEGQAYLKTYSNAVIKNVEIRSDKNNEVVLYGRFNDVDVYSNADIKTKEDTSAIIRAKNSEAKLNAKIYVP
EDTDIKVREFKEKNISGYGRKKALF

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG2247

COG function: function code M; Putative cell wall-binding domain

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 64016; Mature: 64016

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKGKKFLSICITTMLSLTTAITTTKTSAAELAKITRICGVDRYQTAVKISQNGWKSGS
CCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCC
DYAILASGEDYADALCATSLAKAKDAPIFLTKKNSLNEETLKELKRLGVKKLYIIGGYGS
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCC
ISEEIENKVKSVVKNTERLQGKDRYETSVKIAEKLGESNKVILTSGEGYADALSIAPVAA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCHHHH
IKGIPVVLTKVRELPTSTKEYIKSIGATTTYVIGGTEAISDTVKNLVPSPERIYGQDRFE
HCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHCCCCCCC
TNSEIIKAFAIEFDFENAYVALGVGSTGKEFADGLSASALSAKNRAPVILTGQTLNSVTR
CHHHEEEEEEEEEECCCEEEEEEECCCCHHHHCCCCHHHHCCCCCCCEEEECCHHHHHHH
DLAREKLYPTTNLTVLGGVKNISDTAVGEMKVTGEIIKTEKELEDKNIDGNLAITVKNAE
HHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCEEEEEEECCC
LRDSKIKGNLYIENDNILLSNVKVDGTIYINPGSENTCKLENVMANKIIVLSGREEGINL
CCCCCCCCEEEEECCCEEEEEEEEEEEEEECCCCCCCEEHHHCCCCEEEEEECCCCCCEE
ISTKAEKLDILNRDNARVILEQDTDISETKSLSSAILQVEDGCFGEVIIPETLNEKTIQF
EECCHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCEECCCCCCCCEEEE
KGNFDKTIKVEGQAYLKTYSNAVIKNVEIRSDKNNEVVLYGRFNDVDVYSNADIKTKEDT
ECCCCCEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCC
SAIIRAKNSEAKLNAKIYVPEDTDIKVREFKEKNISGYGRKKALF
CEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFKKGKKFLSICITTMLSLTTAITTTKTSAAELAKITRICGVDRYQTAVKISQNGWKSGS
CCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCC
DYAILASGEDYADALCATSLAKAKDAPIFLTKKNSLNEETLKELKRLGVKKLYIIGGYGS
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCC
ISEEIENKVKSVVKNTERLQGKDRYETSVKIAEKLGESNKVILTSGEGYADALSIAPVAA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCHHHH
IKGIPVVLTKVRELPTSTKEYIKSIGATTTYVIGGTEAISDTVKNLVPSPERIYGQDRFE
HCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHCCCCCCC
TNSEIIKAFAIEFDFENAYVALGVGSTGKEFADGLSASALSAKNRAPVILTGQTLNSVTR
CHHHEEEEEEEEEECCCEEEEEEECCCCHHHHCCCCHHHHCCCCCCCEEEECCHHHHHHH
DLAREKLYPTTNLTVLGGVKNISDTAVGEMKVTGEIIKTEKELEDKNIDGNLAITVKNAE
HHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCEEEEEEECCC
LRDSKIKGNLYIENDNILLSNVKVDGTIYINPGSENTCKLENVMANKIIVLSGREEGINL
CCCCCCCCEEEEECCCEEEEEEEEEEEEEECCCCCCCEEHHHCCCCEEEEEECCCCCCEE
ISTKAEKLDILNRDNARVILEQDTDISETKSLSSAILQVEDGCFGEVIIPETLNEKTIQF
EECCHHHHHEECCCCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCEECCCCCCCCEEEE
KGNFDKTIKVEGQAYLKTYSNAVIKNVEIRSDKNNEVVLYGRFNDVDVYSNADIKTKEDT
ECCCCCEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCC
SAIIRAKNSEAKLNAKIYVPEDTDIKVREFKEKNISGYGRKKALF
CEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]