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The map label for this gene is iolJ [H]

Identifier: 28210249

GI number: 28210249

Start: 514113

End: 514958

Strand: Direct

Name: iolJ [H]

Synonym: CTC00507

Alternate gene names: 28210249

Gene position: 514113-514958 (Clockwise)

Preceding gene: 28210248

Following gene: 28210250

Centisome position: 18.37

GC content: 31.32

Gene sequence:

>846_bases
ATGTCGATTGTAAAAATGAAACAACTTTTAAAAAATGCTCAAAAAGGTCAGTATGGAATAGGGGCTTTTAGTATATCCAA
CATGGAAACCATTATTGGAGCTGTAAGAGCAGCTGAGGAGTTAAAATCTCCAATAATACTTCAAATTGCGGAATCTAGAC
TAAAGCATTCGCCCCTTTACTTAATAGGACCGGTAATGATTGAAGCAGCTAAAAAATCATCTGTTCCAATAGCGGTACAT
TTAGATCATGGCGAAAGCATTGATACCATAAAAGAAGCTTTAGATTTAGGTTTTACTTCTGTAATGTATGACGGTTCTCA
TTTTAATTTAAGTAAAAACATTGAAAACACAAAAGAAGTTGTAAGTTTAACTAAAGAATATGGAGCTTCTGTAGAAGGAG
AAGTTGGAAAGGTTGGAAAAAAAGAAGATGGTTTAGGAGATGACACTATATCAATAACAACTGTAGAAGATGCGGAAAAG
TTTTATAAAGAGACAAATGTAGATGCTTTAGCTATAGCTATTGGAAATGTTCATGGTATGTATGTTGGGAAACCTAATCT
TGACATCAAAAGGTTAATAGAGGTTAATAGTAATATAGAAGTGCCTTTAGTTTTACATGGTGGATCAGGTATTACAAAAG
AGGAGTTTAAAAATTGTATTAAAAACGGTATAACCAAAATAAATGTAGCCACAGCAACTTTTAATAGTGTGATAGATAAG
GTAAATGAATTATTTAAAACTTCTAATTCAGTTGATTACTTTACTTATCATGATGTTGTTATTAATGCAGCCTATGATAA
CGTAAAAAAACATATAGACATATTTGGTAGTAGTAACAAAGCATAA

Upstream 100 bases:

>100_bases
AAGATATTGTTAATATATATAAACAGTTGTATATATAAAGAATTGAGAAGTTAAAGTTCTTATATTCCTAATATGGTTTT
GAGAAAAAGGAGTAAATATC

Downstream 100 bases:

>100_bases
ATTTTAAGGGGGTATTTTAGTGAATTATATTAAGTTTGACGAAAAAAAACCACTAGATGTAATTCCTATTGGAAGGGTAA
CCATAGATTTTAATCCTAAT

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: DKGP aldolase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSIVKMKQLLKNAQKGQYGIGAFSISNMETIIGAVRAAEELKSPIILQIAESRLKHSPLYLIGPVMIEAAKKSSVPIAVH
LDHGESIDTIKEALDLGFTSVMYDGSHFNLSKNIENTKEVVSLTKEYGASVEGEVGKVGKKEDGLGDDTISITTVEDAEK
FYKETNVDALAIAIGNVHGMYVGKPNLDIKRLIEVNSNIEVPLVLHGGSGITKEEFKNCIKNGITKINVATATFNSVIDK
VNELFKTSNSVDYFTYHDVVINAAYDNVKKHIDIFGSSNKA

Sequences:

>Translated_281_residues
MSIVKMKQLLKNAQKGQYGIGAFSISNMETIIGAVRAAEELKSPIILQIAESRLKHSPLYLIGPVMIEAAKKSSVPIAVH
LDHGESIDTIKEALDLGFTSVMYDGSHFNLSKNIENTKEVVSLTKEYGASVEGEVGKVGKKEDGLGDDTISITTVEDAEK
FYKETNVDALAIAIGNVHGMYVGKPNLDIKRLIEVNSNIEVPLVLHGGSGITKEEFKNCIKNGITKINVATATFNSVIDK
VNELFKTSNSVDYFTYHDVVINAAYDNVKKHIDIFGSSNKA
>Mature_280_residues
SIVKMKQLLKNAQKGQYGIGAFSISNMETIIGAVRAAEELKSPIILQIAESRLKHSPLYLIGPVMIEAAKKSSVPIAVHL
DHGESIDTIKEALDLGFTSVMYDGSHFNLSKNIENTKEVVSLTKEYGASVEGEVGKVGKKEDGLGDDTISITTVEDAEKF
YKETNVDALAIAIGNVHGMYVGKPNLDIKRLIEVNSNIEVPLVLHGGSGITKEEFKNCIKNGITKINVATATFNSVIDKV
NELFKTSNSVDYFTYHDVVINAAYDNVKKHIDIFGSSNKA

Specific function: Produces dihydroxyacetone phosphate (DHAP or glycerone phosphate) and malonic semialdehyde (MSA or 3-oxopropanoate) from 6-phospho-5-dehydro-2-deoxy-D-gluconate (DKGP) [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family. IolJ subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=283, Percent_Identity=36.7491166077738, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082041, Length=284, Percent_Identity=35.9154929577465, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1788072, Length=282, Percent_Identity=32.9787234042553, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789293, Length=293, Percent_Identity=25.5972696245734, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322790, Length=273, Percent_Identity=27.1062271062271, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.29 [H]

Molecular weight: Translated: 30576; Mature: 30445

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVKMKQLLKNAQKGQYGIGAFSISNMETIIGAVRAAEELKSPIILQIAESRLKHSPLY
CCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCEE
LIGPVMIEAAKKSSVPIAVHLDHGESIDTIKEALDLGFTSVMYDGSHFNLSKNIENTKEV
EEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHEEECCCCCEECCCCHHHHHH
VSLTKEYGASVEGEVGKVGKKEDGLGDDTISITTVEDAEKFYKETNVDALAIAIGNVHGM
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEEECCCCEE
YVGKPNLDIKRLIEVNSNIEVPLVLHGGSGITKEEFKNCIKNGITKINVATATFNSVIDK
EECCCCCCHHHHEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VNELFKTSNSVDYFTYHDVVINAAYDNVKKHIDIFGSSNKA
HHHHHHCCCCCEEEEEEHEEEEHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
SIVKMKQLLKNAQKGQYGIGAFSISNMETIIGAVRAAEELKSPIILQIAESRLKHSPLY
CHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCEE
LIGPVMIEAAKKSSVPIAVHLDHGESIDTIKEALDLGFTSVMYDGSHFNLSKNIENTKEV
EEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHEEECCCCCEECCCCHHHHHH
VSLTKEYGASVEGEVGKVGKKEDGLGDDTISITTVEDAEKFYKETNVDALAIAIGNVHGM
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEEECCCCEE
YVGKPNLDIKRLIEVNSNIEVPLVLHGGSGITKEEFKNCIKNGITKINVATATFNSVIDK
EECCCCCCHHHHEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VNELFKTSNSVDYFTYHDVVINAAYDNVKKHIDIFGSSNKA
HHHHHHCCCCCEEEEEEHEEEEHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA