Click here to switch to the map view.

The map label for this gene is eno

Identifier: 28210138

GI number: 28210138

Start: 372258

End: 373553

Strand: Direct

Name: eno

Synonym: CTC00382

Alternate gene names: 28210138

Gene position: 372258-373553 (Clockwise)

Preceding gene: 28210137

Following gene: 28210139

Centisome position: 13.3

GC content: 33.02

Gene sequence:

>1296_bases
ATGAAAAATTATATTGAAATTATCGATGTATCAGCAAGACAAGTACTTGATTCAAGATCAAATCCAACAGTTGAGGTAGA
TGTAATCCTAGAAGATGGAACTACAGGAAGAGCAGCTGTACCATCAGGAGCATCAACAGGGATTTTTGAAGCTGTTGAAT
TAAGAGATGGTGATAAATCAGTATATAATGGAAAAGGTGTTTTAAAGGCAATAGAAAATGTAAACACTATAATTGCAGAA
GAATTAGTTGGTATGAATGTTTTAGATCAAATTGCAATAGACAAAACAATGATAGAATTAGATGGAACAGATAATAAAGG
TAAATTAGGAGCTAATGCTATGCTTGGAGTATCTCTAGCATGTGCAAAAGCTGCAGCAGAATATTTAGGATTAAGCCTAT
ATCAATATATTGGAGGAGTAAATGCAAAGGTATTACCAGTTCCAATGATGAACATAATAAATGGAGGAGAGCATGCAGAC
AACAATGTAGATCTTCAAGAATTTATGATAATGCCTGCAGGAGCATCAAGTTTTAGTGAAGCTTTAAGAATGTGTTCAGA
AGTATATCATAGCTTAAAATCTTTATTAAAAGCTAAAGGATATGATACAGGTGTAGGCGATGAAGGTGGATTTGCTCCAA
ATCTAAATTCTAATGAAGAAGCTATTCAAGTTATAGTAGAAGCTATAGAAAAAGCTGGATATAAACCAGGAGTAGAAATA
TTTATTGCATTAGACCCAGCGTCTTCAGAAATATTTGAAGATGGAAAATACAATTTAAAGGGAGAGGGAAAAGTTCTTAC
TCCACAAGAAATGGTAGATTATTATGCTAATTTAGTAGCTAAATATCCAATCATATCCATAGAAGATGGTATGGCAGAAG
AAGATTGGGAAGGTTGGAGACTTATAACTGAAAGATTAGGAACAAAAATTCAGTTAGTAGGAGATGATCTATTTGTAACT
AATACTGATAGGTTAACTATGGGAATTGAAAGAGGAGTAGCAAATTCAATTCTTATTAAGCTAAACCAAATAGGAACTTT
AACTGAAACTTTAAATGCTATAGAAATGGCTGAAAGAGCAGGATATACTGCGGTAGTTTCACATAGATCAGGAGAAACTG
AAGATACAACTATAGCAGACTTAGTTGTAGCTGTAAATGCAGGTCAAATAAAGACTGGTGCACCAGCAAGAAGTGAAAGG
GTTGCTAAATACAATCAATTATTAAGAATTGAAGATGAGTTAGGAGAAATGGGAGAATACAGAGGATTAAAAGCTTTCTA
TAATATTAAAAAGTAA

Upstream 100 bases:

>100_bases
CATAGCACCAACTATGCTTACAGAAATGGGTGTCAAAATACCAGAAGAAATGACTGGAAGAAACTTAATAGAAGGAAAGT
AAAGGGAGGATATATAAATT

Downstream 100 bases:

>100_bases
AAAAATATTAAATTGAATAAAATTCGGGGAAATGTTAATAATTAAGATAATATTTCTCCGAATTTTGTGTTAAATTCAAA
TATAAATTATTCAGTTCCAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKNYIEIIDVSARQVLDSRSNPTVEVDVILEDGTTGRAAVPSGASTGIFEAVELRDGDKSVYNGKGVLKAIENVNTIIAE
ELVGMNVLDQIAIDKTMIELDGTDNKGKLGANAMLGVSLACAKAAAEYLGLSLYQYIGGVNAKVLPVPMMNIINGGEHAD
NNVDLQEFMIMPAGASSFSEALRMCSEVYHSLKSLLKAKGYDTGVGDEGGFAPNLNSNEEAIQVIVEAIEKAGYKPGVEI
FIALDPASSEIFEDGKYNLKGEGKVLTPQEMVDYYANLVAKYPIISIEDGMAEEDWEGWRLITERLGTKIQLVGDDLFVT
NTDRLTMGIERGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEDELGEMGEYRGLKAFYNIKK

Sequences:

>Translated_431_residues
MKNYIEIIDVSARQVLDSRSNPTVEVDVILEDGTTGRAAVPSGASTGIFEAVELRDGDKSVYNGKGVLKAIENVNTIIAE
ELVGMNVLDQIAIDKTMIELDGTDNKGKLGANAMLGVSLACAKAAAEYLGLSLYQYIGGVNAKVLPVPMMNIINGGEHAD
NNVDLQEFMIMPAGASSFSEALRMCSEVYHSLKSLLKAKGYDTGVGDEGGFAPNLNSNEEAIQVIVEAIEKAGYKPGVEI
FIALDPASSEIFEDGKYNLKGEGKVLTPQEMVDYYANLVAKYPIISIEDGMAEEDWEGWRLITERLGTKIQLVGDDLFVT
NTDRLTMGIERGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEDELGEMGEYRGLKAFYNIKK
>Mature_431_residues
MKNYIEIIDVSARQVLDSRSNPTVEVDVILEDGTTGRAAVPSGASTGIFEAVELRDGDKSVYNGKGVLKAIENVNTIIAE
ELVGMNVLDQIAIDKTMIELDGTDNKGKLGANAMLGVSLACAKAAAEYLGLSLYQYIGGVNAKVLPVPMMNIINGGEHAD
NNVDLQEFMIMPAGASSFSEALRMCSEVYHSLKSLLKAKGYDTGVGDEGGFAPNLNSNEEAIQVIVEAIEKAGYKPGVEI
FIALDPASSEIFEDGKYNLKGEGKVLTPQEMVDYYANLVAKYPIISIEDGMAEEDWEGWRLITERLGTKIQLVGDDLFVT
NTDRLTMGIERGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEDELGEMGEYRGLKAFYNIKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.8348623853211, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=51.9630484988453, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=51.9630484988453, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=46.8677494199536, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=27.0588235294118, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=27.0588235294118, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=27.0588235294118, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=61.7924528301887, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.0603248259861, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.0603248259861, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=269, Percent_Identity=42.3791821561338, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=428, Percent_Identity=54.2056074766355, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=51.2643678160919, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=51.2643678160919, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=51.0344827586207, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321968, Length=428, Percent_Identity=53.7383177570093, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=52.4027459954233, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=52.4027459954233, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=52.4027459954233, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=52.4027459954233, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=52.7906976744186, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=52.7906976744186, Blast_Score=418, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CLOTE (Q898R0)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781082.1
- ProteinModelPortal:   Q898R0
- SMR:   Q898R0
- GeneID:   1059961
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00382
- NMPDR:   fig|212717.1.peg.304
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q898R0
- ProtClustDB:   PRK00077
- BioCyc:   CTET212717:CTC_00382-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46578; Mature: 46578

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 208-208 ACT_SITE 340-340 BINDING 158-158 BINDING 167-167 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYIEIIDVSARQVLDSRSNPTVEVDVILEDGTTGRAAVPSGASTGIFEAVELRDGDKS
CCCCEEEEEHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCC
VYNGKGVLKAIENVNTIIAEELVGMNVLDQIAIDKTMIELDGTDNKGKLGANAMLGVSLA
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHH
CAKAAAEYLGLSLYQYIGGVNAKVLPVPMMNIINGGEHADNNVDLQEFMIMPAGASSFSE
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHH
ALRMCSEVYHSLKSLLKAKGYDTGVGDEGGFAPNLNSNEEAIQVIVEAIEKAGYKPGVEI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FIALDPASSEIFEDGKYNLKGEGKVLTPQEMVDYYANLVAKYPIISIEDGMAEEDWEGWR
EEEECCCCHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHH
LITERLGTKIQLVGDDLFVTNTDRLTMGIERGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHHCCEEEEECCEEEEECCCCEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEDELGEMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHEEEHHHHCCHHHC
RGLKAFYNIKK
CCCHHHHCCCC
>Mature Secondary Structure
MKNYIEIIDVSARQVLDSRSNPTVEVDVILEDGTTGRAAVPSGASTGIFEAVELRDGDKS
CCCCEEEEEHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCC
VYNGKGVLKAIENVNTIIAEELVGMNVLDQIAIDKTMIELDGTDNKGKLGANAMLGVSLA
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHH
CAKAAAEYLGLSLYQYIGGVNAKVLPVPMMNIINGGEHADNNVDLQEFMIMPAGASSFSE
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHH
ALRMCSEVYHSLKSLLKAKGYDTGVGDEGGFAPNLNSNEEAIQVIVEAIEKAGYKPGVEI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
FIALDPASSEIFEDGKYNLKGEGKVLTPQEMVDYYANLVAKYPIISIEDGMAEEDWEGWR
EEEECCCCHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHH
LITERLGTKIQLVGDDLFVTNTDRLTMGIERGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHHCCEEEEECCEEEEECCCCEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEDELGEMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHEEEHHHHCCHHHC
RGLKAFYNIKK
CCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129