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The map label for this gene is pgk

Identifier: 28210135

GI number: 28210135

Start: 368702

End: 369907

Strand: Direct

Name: pgk

Synonym: CTC00379

Alternate gene names: 28210135

Gene position: 368702-369907 (Clockwise)

Preceding gene: 28210134

Following gene: 28210136

Centisome position: 13.17

GC content: 32.5

Gene sequence:

>1206_bases
GTGGTTTATGTGGCATTTAATAAAAAAACCATTGAAGACATTGATGTTAAAGGAAGAAAAGTTTTAGTAAGATGTGATTT
TAATGTACCTTTAAAAGATGGAAAAATAACAGATGAAAATAGATTAATGGGAGCACTACCAACTATTAAATATATTATGG
AAAAGGGCGGTAAAGTTATTTTATGTTCTCACCTTGGTAAACCAAAAGGAGAGCCTAAACAGGAATTATCTCTAGCACCA
GTAGCTAAAAGATTATCTGAGCTTTTAAATAAGGAAGTTTTATTTCCAGCAGATAATGAAGTAGTTGGAGAAAATGCTAA
AAAAGCAGTAGAAAATATGAAAGATGGAGATGTAATCCTATTACAAAATACAAGATATAGAAAAGAAGAAACTAAGAATG
AAGAAACCTTTTCAAAAGAATTAGCATCTCTTGCAGATATATTTGTAAATGATGCTTTTGGAACAGCACATAGAGCACAT
TGCTCTACAGTTGGAGTAACAGAATTTGTTGCAACATCAGTATGTGGATACTTAATCCAAAAAGAATTAAAGTTTTTAGG
TAATGCTGTTGAAAATCCACAAAGACCTTTTATATCAATTCTTGGAGGAGCTAAAGTATCTGATAAAATAAATGTAATAA
ATAATTTATTAGAAAAAGTTGATACTCTAATTATAGGTGGAGGAATGAGCTATACTTTCCAAAAGGCTCAAGGGTACACA
ATAGGAAGCTCTCTTTTAGAAGAAGACAAAATAGATTATGCAAAAGAAATGATAGAAAAGGCAAAAGAAAAGGGAGTAAA
ATTACTTCTACCTGTAGATAATGTAGCAGCTGAAAAATTTGCAGAAGATGCAGAAGCTATTATAACAGAAGATCAAAATA
TTAAAGAAGGATATATGGGACTTGATATAGGACCTAAAACTTCAAAACTTTATTCACAAGAGGTTCAAAGCGCTAAGACT
GTAGTTTGGAATGGACCTATGGGTGTATTTGAATTTGAAAAATTTGCAAAAGGTACTATAGAAGTAGCGAAGGCCATGGC
TGAATCACAAGCTACAACCATAATAGGTGGAGGAGATAGTGCTGCGGCTGTAAATCAACTTGGATTTGGGGATAAAATGA
CCCATATATCTACAGGTGGAGGAGCTTCATTAGAATTTTTAGAAGGAAAAGAGCTTCCAGGAATAGCAGCACTTAACGAT
AAATAG

Upstream 100 bases:

>100_bases
ATCGAGTAAATAAGTCATACTTATAGTTAAAAACTAAGGGGGTTTTTGCTTTGTAGTAAAGCTATAAGACAAAACCCTCT
TTTGCCTATATAAAGAAAGG

Downstream 100 bases:

>100_bases
GTTTTTGAAAGATTGAAAGGGGTGTATGGATTGCGTAAAGCAATAATAGCAGGAAACTGGAAAATGCATAATACTATAGA
TGAATCAGTGAAATTAGTTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MVYVAFNKKTIEDIDVKGRKVLVRCDFNVPLKDGKITDENRLMGALPTIKYIMEKGGKVILCSHLGKPKGEPKQELSLAP
VAKRLSELLNKEVLFPADNEVVGENAKKAVENMKDGDVILLQNTRYRKEETKNEETFSKELASLADIFVNDAFGTAHRAH
CSTVGVTEFVATSVCGYLIQKELKFLGNAVENPQRPFISILGGAKVSDKINVINNLLEKVDTLIIGGGMSYTFQKAQGYT
IGSSLLEEDKIDYAKEMIEKAKEKGVKLLLPVDNVAAEKFAEDAEAIITEDQNIKEGYMGLDIGPKTSKLYSQEVQSAKT
VVWNGPMGVFEFEKFAKGTIEVAKAMAESQATTIIGGGDSAAAVNQLGFGDKMTHISTGGGASLEFLEGKELPGIAALND
K

Sequences:

>Translated_401_residues
MVYVAFNKKTIEDIDVKGRKVLVRCDFNVPLKDGKITDENRLMGALPTIKYIMEKGGKVILCSHLGKPKGEPKQELSLAP
VAKRLSELLNKEVLFPADNEVVGENAKKAVENMKDGDVILLQNTRYRKEETKNEETFSKELASLADIFVNDAFGTAHRAH
CSTVGVTEFVATSVCGYLIQKELKFLGNAVENPQRPFISILGGAKVSDKINVINNLLEKVDTLIIGGGMSYTFQKAQGYT
IGSSLLEEDKIDYAKEMIEKAKEKGVKLLLPVDNVAAEKFAEDAEAIITEDQNIKEGYMGLDIGPKTSKLYSQEVQSAKT
VVWNGPMGVFEFEKFAKGTIEVAKAMAESQATTIIGGGDSAAAVNQLGFGDKMTHISTGGGASLEFLEGKELPGIAALND
K
>Mature_401_residues
MVYVAFNKKTIEDIDVKGRKVLVRCDFNVPLKDGKITDENRLMGALPTIKYIMEKGGKVILCSHLGKPKGEPKQELSLAP
VAKRLSELLNKEVLFPADNEVVGENAKKAVENMKDGDVILLQNTRYRKEETKNEETFSKELASLADIFVNDAFGTAHRAH
CSTVGVTEFVATSVCGYLIQKELKFLGNAVENPQRPFISILGGAKVSDKINVINNLLEKVDTLIIGGGMSYTFQKAQGYT
IGSSLLEEDKIDYAKEMIEKAKEKGVKLLLPVDNVAAEKFAEDAEAIITEDQNIKEGYMGLDIGPKTSKLYSQEVQSAKT
VVWNGPMGVFEFEKFAKGTIEVAKAMAESQATTIIGGGDSAAAVNQLGFGDKMTHISTGGGASLEFLEGKELPGIAALND
K

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=48.4412470023981, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=46.875, Blast_Score=358, Evalue=5e-99,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=42.3857868020305, Blast_Score=323, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=49.8800959232614, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=48.9156626506024, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=46.1538461538462, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=43.855421686747, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CLOTE (Q898R3)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781079.1
- ProteinModelPortal:   Q898R3
- SMR:   Q898R3
- GeneID:   1059957
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00379
- NMPDR:   fig|212717.1.peg.301
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q898R3
- ProtClustDB:   PRK00073
- BioCyc:   CTET212717:CTC_00379-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43579; Mature: 43579

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 41-41 BINDING 125-125 BINDING 158-158 BINDING 209-209 BINDING 300-300 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYVAFNKKTIEDIDVKGRKVLVRCDFNVPLKDGKITDENRLMGALPTIKYIMEKGGKVI
CEEEEECCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
LCSHLGKPKGEPKQELSLAPVAKRLSELLNKEVLFPADNEVVGENAKKAVENMKDGDVIL
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHCCHHHHHHHCCCCCCEEE
LQNTRYRKEETKNEETFSKELASLADIFVNDAFGTAHRAHCSTVGVTEFVATSVCGYLIQ
EECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
KELKFLGNAVENPQRPFISILGGAKVSDKINVINNLLEKVDTLIIGGGMSYTFQKAQGYT
HHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
IGSSLLEEDKIDYAKEMIEKAKEKGVKLLLPVDNVAAEKFAEDAEAIITEDQNIKEGYMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDIGPKTSKLYSQEVQSAKTVVWNGPMGVFEFEKFAKGTIEVAKAMAESQATTIIGGGDS
CCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
AAAVNQLGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK
HHHHHHCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVYVAFNKKTIEDIDVKGRKVLVRCDFNVPLKDGKITDENRLMGALPTIKYIMEKGGKVI
CEEEEECCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
LCSHLGKPKGEPKQELSLAPVAKRLSELLNKEVLFPADNEVVGENAKKAVENMKDGDVIL
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHCCHHHHHHHCCCCCCEEE
LQNTRYRKEETKNEETFSKELASLADIFVNDAFGTAHRAHCSTVGVTEFVATSVCGYLIQ
EECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
KELKFLGNAVENPQRPFISILGGAKVSDKINVINNLLEKVDTLIIGGGMSYTFQKAQGYT
HHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCC
IGSSLLEEDKIDYAKEMIEKAKEKGVKLLLPVDNVAAEKFAEDAEAIITEDQNIKEGYMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDIGPKTSKLYSQEVQSAKTVVWNGPMGVFEFEKFAKGTIEVAKAMAESQATTIIGGGDS
CCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
AAAVNQLGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK
HHHHHHCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129