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The map label for this gene is tpiA

Identifier: 28210136

GI number: 28210136

Start: 369920

End: 370687

Strand: Direct

Name: tpiA

Synonym: CTC00380

Alternate gene names: 28210136

Gene position: 369920-370687 (Clockwise)

Preceding gene: 28210135

Following gene: 28210137

Centisome position: 13.21

GC content: 33.46

Gene sequence:

>768_bases
TTGAAAGGGGTGTATGGATTGCGTAAAGCAATAATAGCAGGAAACTGGAAAATGCATAATACTATAGATGAATCAGTGAA
ATTAGTTGAGGAATTAATTCCGAAAGTTAAAGAAGCAGAATGTGAAGTAGTAGTTTGTCCACCTTTTATATGCCTTCCAA
AGATTAGAGAAATTACAGAAGGAACAAATATAAAAGTTGGAGCTCAGAATATGTATTTCGAAGAGAAAGGAGCTTTTACA
GGAGAGGTATCTCCCCTAATGCTTGAAAAGTTAAATATAGATTATGTAATAATAGGTCATAGTGAAAGAAGACAATATTT
TAAAGAAACTGACCAAACAGTTAACAAAAAGCTTAAAGCAGCCTTTGAACACAATTTAATTCCAATACTTTGTGTAGGAG
AAACTTTAGATGAAAAAGAAAATGGTGTAACCGAAGAAGTTGTATCAAAACAAGTAAAACTTGGTCTTTTTGAATTAAAA
GAAGAACAAGTAGAGAAGCTAGTAATAGCTTATGAACCAATATGGGCTATAGGTACAGGAAAAACAGCAACCTCAGAGGA
GGCAAATGAAGTTATAGCACTTATAAGAAGTACCGTAAAAAGCCTATATGGAGACAAAGTGGCAGAAAGCCTAAGAATAC
AATATGGTGGTTCAGTAAAACCTTCAACTATAAAAGAACAAATGTCTATGTCAGATATAGACGGAGCTTTAGTAGGAGGA
GCAAGTCTTAAAGCAAATGACTTCTCAGCTATAGTAAACTACAAATAG

Upstream 100 bases:

>100_bases
GACCCATATATCTACAGGTGGAGGAGCTTCATTAGAATTTTTAGAAGGAAAAGAGCTTCCAGGAATAGCAGCACTTAACG
ATAAATAGGTTTTTGAAAGA

Downstream 100 bases:

>100_bases
GGGGAAGACTATGAATAAAAAACCAGTAATGCTTATGATTTTAGACGGTTTTGGCATAACAAATCATGAAGATGGAAATG
CAGTAAAGATGGCAAATAAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKGVYGLRKAIIAGNWKMHNTIDESVKLVEELIPKVKEAECEVVVCPPFICLPKIREITEGTNIKVGAQNMYFEEKGAFT
GEVSPLMLEKLNIDYVIIGHSERRQYFKETDQTVNKKLKAAFEHNLIPILCVGETLDEKENGVTEEVVSKQVKLGLFELK
EEQVEKLVIAYEPIWAIGTGKTATSEEANEVIALIRSTVKSLYGDKVAESLRIQYGGSVKPSTIKEQMSMSDIDGALVGG
ASLKANDFSAIVNYK

Sequences:

>Translated_255_residues
MKGVYGLRKAIIAGNWKMHNTIDESVKLVEELIPKVKEAECEVVVCPPFICLPKIREITEGTNIKVGAQNMYFEEKGAFT
GEVSPLMLEKLNIDYVIIGHSERRQYFKETDQTVNKKLKAAFEHNLIPILCVGETLDEKENGVTEEVVSKQVKLGLFELK
EEQVEKLVIAYEPIWAIGTGKTATSEEANEVIALIRSTVKSLYGDKVAESLRIQYGGSVKPSTIKEQMSMSDIDGALVGG
ASLKANDFSAIVNYK
>Mature_255_residues
MKGVYGLRKAIIAGNWKMHNTIDESVKLVEELIPKVKEAECEVVVCPPFICLPKIREITEGTNIKVGAQNMYFEEKGAFT
GEVSPLMLEKLNIDYVIIGHSERRQYFKETDQTVNKKLKAAFEHNLIPILCVGETLDEKENGVTEEVVSKQVKLGLFELK
EEQVEKLVIAYEPIWAIGTGKTATSEEANEVIALIRSTVKSLYGDKVAESLRIQYGGSVKPSTIKEQMSMSDIDGALVGG
ASLKANDFSAIVNYK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=45.1612903225806, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=45.1612903225806, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=43.8247011952191, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17536593, Length=248, Percent_Identity=42.3387096774194, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=41.9354838709677, Blast_Score=181, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=42.741935483871, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=42.741935483871, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=42.741935483871, Blast_Score=188, Evalue=2e-48,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CLOTE (Q898R2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781080.1
- ProteinModelPortal:   Q898R2
- SMR:   Q898R2
- GeneID:   1059958
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00380
- NMPDR:   fig|212717.1.peg.302
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q898R2
- ProtClustDB:   PRK00042
- BioCyc:   CTET212717:CTC_00380-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28288; Mature: 28288

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 15-15 BINDING 17-17

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVYGLRKAIIAGNWKMHNTIDESVKLVEELIPKVKEAECEVVVCPPFICLPKIREITE
CCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCC
GTNIKVGAQNMYFEEKGAFTGEVSPLMLEKLNIDYVIIGHSERRQYFKETDQTVNKKLKA
CCCEEECCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
AFEHNLIPILCVGETLDEKENGVTEEVVSKQVKLGLFELKEEQVEKLVIAYEPIWAIGTG
HHHCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECC
KTATSEEANEVIALIRSTVKSLYGDKVAESLRIQYGGSVKPSTIKEQMSMSDIDGALVGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCEEECC
ASLKANDFSAIVNYK
CCCCCCCCEEEECCC
>Mature Secondary Structure
MKGVYGLRKAIIAGNWKMHNTIDESVKLVEELIPKVKEAECEVVVCPPFICLPKIREITE
CCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCC
GTNIKVGAQNMYFEEKGAFTGEVSPLMLEKLNIDYVIIGHSERRQYFKETDQTVNKKLKA
CCCEEECCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
AFEHNLIPILCVGETLDEKENGVTEEVVSKQVKLGLFELKEEQVEKLVIAYEPIWAIGTG
HHHCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECC
KTATSEEANEVIALIRSTVKSLYGDKVAESLRIQYGGSVKPSTIKEQMSMSDIDGALVGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCEEECC
ASLKANDFSAIVNYK
CCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129