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The map label for this gene is hemK [H]

Identifier: 28210062

GI number: 28210062

Start: 285272

End: 286150

Strand: Direct

Name: hemK [H]

Synonym: CTC00301

Alternate gene names: 28210062

Gene position: 285272-286150 (Clockwise)

Preceding gene: 28210061

Following gene: 28210063

Centisome position: 10.19

GC content: 26.51

Gene sequence:

>879_bases
ATGGTTATAAAAAATTGTGAAACCATACAAGACCTTTTATCAGAAGGCATTAAATCTTTAAAAGAGGTAGGTATAGAAAG
TTATATATTAGAGAGCCAACTATTACTAGGAAAAGTAATAAATAAAGATAGACTTTTTATAATGATTAATAAGGATTATA
AAGTGTCTAAAGAAGAAAAAGATCAGTATTTAAAACTTATAGATATTAGGAAAGAAAAAATGCCAATAAAATATATACTA
GGTATTTGTGAATTTATGGGGCTTTCCTTTAAAATAAGAGAAGGAGTACTGATTCCAAGACCAGATACAGAAATTCTAGT
AGAAAAAAGCATAGAGATCATAAGAAAAAATCATTATAAGAATATAAGTGATGTATGCTGTGGCAGTGGAATAATTGGCA
TATCTATTGCAACATTTGTTGATAAAGTTAATGTAGAATGTTTTGATGTTGAAGAAATACCATTAAAAGTTACTGAGGAA
AACATTAAATTGAATAGACTAGAAGGGAAAGTGAAAGTTTTTAAAAGTAACTTATTAGAATATGCTATAAAAAATAGTAT
AAAATATGATATGATAGTATCAAATCCTCCATATATTAAAAAAAGAGAAATAACAAATTTGATGGAGGATGTAAAAAACT
ACGAACCACATATAGCCTTAGATGGTGGCGAAGATGGGCTATATTTTTATAAAAATATAGTAAAACAAAGTAAACATCTT
TTAAATTCAGGAGGAACTATAGCTTTTGAAATAGGATATGATCAAAAAGAAGAAGTTAGTTGTATATTAGAAAGTAATGG
CTTTGTTAATATAGAATGTTATAAAGATTTGGCAGGGTTAGATAGAGTTGTTATTGGAACACTTAAAACAACTAATTGA

Upstream 100 bases:

>100_bases
ACTTACCACTAGAGAACCTGACACATTACAAATAGAAGTGGCTATAAAGGCTTTAAAAGGTGCAGAAGGTATAGAAGATT
AAAAAGGAGTAAGCTATAAA

Downstream 100 bases:

>100_bases
TTTAAATACAAAAAATATGATATAATTAATATTTGTTAAAATATATTTACGGAGTGAGAAAGTTATGTTAGATAGATTAA
ATTTTATAGAAAATAAATAT

Product: methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVIKNCETIQDLLSEGIKSLKEVGIESYILESQLLLGKVINKDRLFIMINKDYKVSKEEKDQYLKLIDIRKEKMPIKYIL
GICEFMGLSFKIREGVLIPRPDTEILVEKSIEIIRKNHYKNISDVCCGSGIIGISIATFVDKVNVECFDVEEIPLKVTEE
NIKLNRLEGKVKVFKSNLLEYAIKNSIKYDMIVSNPPYIKKREITNLMEDVKNYEPHIALDGGEDGLYFYKNIVKQSKHL
LNSGGTIAFEIGYDQKEEVSCILESNGFVNIECYKDLAGLDRVVIGTLKTTN

Sequences:

>Translated_292_residues
MVIKNCETIQDLLSEGIKSLKEVGIESYILESQLLLGKVINKDRLFIMINKDYKVSKEEKDQYLKLIDIRKEKMPIKYIL
GICEFMGLSFKIREGVLIPRPDTEILVEKSIEIIRKNHYKNISDVCCGSGIIGISIATFVDKVNVECFDVEEIPLKVTEE
NIKLNRLEGKVKVFKSNLLEYAIKNSIKYDMIVSNPPYIKKREITNLMEDVKNYEPHIALDGGEDGLYFYKNIVKQSKHL
LNSGGTIAFEIGYDQKEEVSCILESNGFVNIECYKDLAGLDRVVIGTLKTTN
>Mature_292_residues
MVIKNCETIQDLLSEGIKSLKEVGIESYILESQLLLGKVINKDRLFIMINKDYKVSKEEKDQYLKLIDIRKEKMPIKYIL
GICEFMGLSFKIREGVLIPRPDTEILVEKSIEIIRKNHYKNISDVCCGSGIIGISIATFVDKVNVECFDVEEIPLKVTEE
NIKLNRLEGKVKVFKSNLLEYAIKNSIKYDMIVSNPPYIKKREITNLMEDVKNYEPHIALDGGEDGLYFYKNIVKQSKHL
LNSGGTIAFEIGYDQKEEVSCILESNGFVNIECYKDLAGLDRVVIGTLKTTN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=228, Percent_Identity=31.5789473684211, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787463, Length=278, Percent_Identity=29.136690647482, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87082085, Length=263, Percent_Identity=27.3764258555133, Blast_Score=98, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=215, Percent_Identity=29.7674418604651, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582226, Length=204, Percent_Identity=31.3725490196078, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 33332; Mature: 33332

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIKNCETIQDLLSEGIKSLKEVGIESYILESQLLLGKVINKDRLFIMINKDYKVSKEEK
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
DQYLKLIDIRKEKMPIKYILGICEFMGLSFKIREGVLIPRPDTEILVEKSIEIIRKNHYK
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHHHHHHCCC
NISDVCCGSGIIGISIATFVDKVNVECFDVEEIPLKVTEENIKLNRLEGKVKVFKSNLLE
CHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEEEECCCHHHHHHHHHHH
YAIKNSIKYDMIVSNPPYIKKREITNLMEDVKNYEPHIALDGGEDGLYFYKNIVKQSKHL
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
LNSGGTIAFEIGYDQKEEVSCILESNGFVNIECYKDLAGLDRVVIGTLKTTN
HCCCCEEEEEECCCCCCCEEEEEECCCEEEEEEHHHHCCCCEEEEEEEECCC
>Mature Secondary Structure
MVIKNCETIQDLLSEGIKSLKEVGIESYILESQLLLGKVINKDRLFIMINKDYKVSKEEK
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
DQYLKLIDIRKEKMPIKYILGICEFMGLSFKIREGVLIPRPDTEILVEKSIEIIRKNHYK
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHHHHHHCCC
NISDVCCGSGIIGISIATFVDKVNVECFDVEEIPLKVTEENIKLNRLEGKVKVFKSNLLE
CHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEEEECCCHHHHHHHHHHH
YAIKNSIKYDMIVSNPPYIKKREITNLMEDVKNYEPHIALDGGEDGLYFYKNIVKQSKHL
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
LNSGGTIAFEIGYDQKEEVSCILESNGFVNIECYKDLAGLDRVVIGTLKTTN
HCCCCEEEEEECCCCCCCEEEEEECCCEEEEEEHHHHCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA