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The map label for this gene is prfA

Identifier: 28210063

GI number: 28210063

Start: 286215

End: 287282

Strand: Direct

Name: prfA

Synonym: CTC00302

Alternate gene names: 28210063

Gene position: 286215-287282 (Clockwise)

Preceding gene: 28210062

Following gene: 28210064

Centisome position: 10.22

GC content: 32.3

Gene sequence:

>1068_bases
ATGTTAGATAGATTAAATTTTATAGAAAATAAATATGAAGAACTTTCAATAAAGATTAGTGACCCTACAGTGATGCAAGA
TCAAAAAGAATGGCAAAAACTGTGTAAAGAACACTCAGATATGGAAACTATAGTGACTACTTATAAAGAATATAAAGAAG
TTTTACAAAGTATAGAAGATAATAAAGAGATGTTAAAAGAAGATATAGAACAAGAGTTAAGAGATATGGTTCAAGAAGAC
ATAAAGGAGCTTGAACAAAGGGTTCAAGAATTAGAACAAGAACTAAAGATGTTATTAGTGCCAAAAGATCCAAATGATGA
AAAGAATGTTTTCATTGAAATAAGAGCAGGTGCTGGAGGAGATGAGGCAGCTCTTTTTGCTGCTAACTTATTTAGAATGT
ATACAAGATATGCTGAAAGACATAATTGGAAGACGGAAGCAGTTAGTGTAAATGAGACGGATATAGGTGGTTTTAAAGAA
ATTGTATTTATGGTTAGAGGAAAAGGAGCCTATAGTAGATTAAAGTACGAAAGTGGAGTTCATAGAGTACAAAGAGTACC
AGATACAGAATCTAGTGGAAGAATCCATACATCTACAGCTACAGTAGCAGTTTTACCAGAAGTAGAAGATGTTGATGTTG
AGATAAACCAAAATGACTTAAGAGTAGATGTGTATAGAGCATCAGGTCATGGAGGACAATGTGTAAATACTACAGACTCA
GCGGTAAGAATAACTCACTTACCTTCAGGATTAGTAGTTACTTGTCAAGATGAGAAATCTCAGCTAAAAAATAAAGAAAA
GGCTATGAAGGTTTTAAAGTCTAGATTATATGATATGTTAGAATCTGAGAGAAGTGCAAGTATTGCGGAAGATAGAAAAA
GTCAAGTAGGAACTGGAGATAGAAGTGAAAGAATTAGAACTTACAATTATCCTCAAGGAAGGGTTACAGATCATAGAATT
GGTTTAACATTATATAAGTTAGAGTCTTTCTTAGATGGAGACATAGAAGAAATGATAGATGGATTAATTACTGTTGAACA
GTCAGAAAGAATGAAAGATATAAGTTAA

Upstream 100 bases:

>100_bases
AGAGTTGTTATTGGAACACTTAAAACAACTAATTGATTTAAATACAAAAAATATGATATAATTAATATTTGTTAAAATAT
ATTTACGGAGTGAGAAAGTT

Downstream 100 bases:

>100_bases
AAAAATATAGGAGAAGATGAGTATGAATATAAATAAAGCTCTAAAGAAGCAAATAAATTCTTATAAAAGATTTATGCTAA
TTATGGGCTTTATTTTTTTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLDRLNFIENKYEELSIKISDPTVMQDQKEWQKLCKEHSDMETIVTTYKEYKEVLQSIEDNKEMLKEDIEQELRDMVQED
IKELEQRVQELEQELKMLLVPKDPNDEKNVFIEIRAGAGGDEAALFAANLFRMYTRYAERHNWKTEAVSVNETDIGGFKE
IVFMVRGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVEDVDVEINQNDLRVDVYRASGHGGQCVNTTDS
AVRITHLPSGLVVTCQDEKSQLKNKEKAMKVLKSRLYDMLESERSASIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHRI
GLTLYKLESFLDGDIEEMIDGLITVEQSERMKDIS

Sequences:

>Translated_355_residues
MLDRLNFIENKYEELSIKISDPTVMQDQKEWQKLCKEHSDMETIVTTYKEYKEVLQSIEDNKEMLKEDIEQELRDMVQED
IKELEQRVQELEQELKMLLVPKDPNDEKNVFIEIRAGAGGDEAALFAANLFRMYTRYAERHNWKTEAVSVNETDIGGFKE
IVFMVRGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVEDVDVEINQNDLRVDVYRASGHGGQCVNTTDS
AVRITHLPSGLVVTCQDEKSQLKNKEKAMKVLKSRLYDMLESERSASIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHRI
GLTLYKLESFLDGDIEEMIDGLITVEQSERMKDIS
>Mature_355_residues
MLDRLNFIENKYEELSIKISDPTVMQDQKEWQKLCKEHSDMETIVTTYKEYKEVLQSIEDNKEMLKEDIEQELRDMVQED
IKELEQRVQELEQELKMLLVPKDPNDEKNVFIEIRAGAGGDEAALFAANLFRMYTRYAERHNWKTEAVSVNETDIGGFKE
IVFMVRGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVEDVDVEINQNDLRVDVYRASGHGGQCVNTTDS
AVRITHLPSGLVVTCQDEKSQLKNKEKAMKVLKSRLYDMLESERSASIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHRI
GLTLYKLESFLDGDIEEMIDGLITVEQSERMKDIS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=282, Percent_Identity=47.1631205673759, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=40.9375, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI166795305, Length=187, Percent_Identity=44.9197860962567, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=47.752808988764, Blast_Score=353, Evalue=8e-99,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.0239520958084, Blast_Score=224, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=38.5454545454545, Blast_Score=190, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=41.9452887537994, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19921226, Length=324, Percent_Identity=44.1358024691358, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CLOTE (Q898Y5)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781007.1
- ProteinModelPortal:   Q898Y5
- SMR:   Q898Y5
- GeneID:   1059878
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00302
- NMPDR:   fig|212717.1.peg.229
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q898Y5
- ProtClustDB:   PRK00591
- BioCyc:   CTET212717:CTC_00302-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR000219
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- Gene3D:   G3DSA:1.20.900.10
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40842; Mature: 40842

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLNFIENKYEELSIKISDPTVMQDQKEWQKLCKEHSDMETIVTTYKEYKEVLQSIED
CCCCHHHHHCCHHHEEEEECCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NKEMLKEDIEQELRDMVQEDIKELEQRVQELEQELKMLLVPKDPNDEKNVFIEIRAGAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCC
DEAALFAANLFRMYTRYAERHNWKTEAVSVNETDIGGFKEIVFMVRGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVEDVDVEINQNDLRVDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPSGLVVTCQDEKSQLKNKEKAMKVLKSRLYDMLESERSASIAEDRKSQVGTGD
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRVTDHRIGLTLYKLESFLDGDIEEMIDGLITVEQSERMKDIS
CCCCCEECCCCCCCCCCHHHCHHHHHHHHHHCCCHHHHHHHHEEECHHHHCCCCC
>Mature Secondary Structure
MLDRLNFIENKYEELSIKISDPTVMQDQKEWQKLCKEHSDMETIVTTYKEYKEVLQSIED
CCCCHHHHHCCHHHEEEEECCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NKEMLKEDIEQELRDMVQEDIKELEQRVQELEQELKMLLVPKDPNDEKNVFIEIRAGAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCC
DEAALFAANLFRMYTRYAERHNWKTEAVSVNETDIGGFKEIVFMVRGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVEDVDVEINQNDLRVDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPSGLVVTCQDEKSQLKNKEKAMKVLKSRLYDMLESERSASIAEDRKSQVGTGD
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRVTDHRIGLTLYKLESFLDGDIEEMIDGLITVEQSERMKDIS
CCCCCEECCCCCCCCCCHHHCHHHHHHHHHHCCCHHHHHHHHEEECHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129