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The map label for this gene is ddl

Identifier: 28210053

GI number: 28210053

Start: 275925

End: 277001

Strand: Direct

Name: ddl

Synonym: CTC00291

Alternate gene names: 28210053

Gene position: 275925-277001 (Clockwise)

Preceding gene: 28210052

Following gene: 28210054

Centisome position: 9.86

GC content: 28.6

Gene sequence:

>1077_bases
GTGCTTTTAGCACAACAATTAAATTTGTATATATTAAAGGGAGAGATAATAATGAAGAAAATAAAAATAGCCATAGTTTT
TGGTGGTCAATCCACAGAACATGAAGTTTCCAGAAATTCAGTCACATCAATATTGAAAAATATTAATAGAGATAAATACG
AGATATGCCCTATAGGAATAACTAAAGAGGGTAAATGGTTTCAATATACTGGAGATATAAATAATATTAAAAATGGACAA
TGGGAAAAGGACAATAAAAATAAATTAGAGAATGGATATAATGTACTATTTAATAAAGAAGTAGAATTAGTATTCCCAGT
ATTGCATGGATTATATGGAGAAGACGGAACTATACAAGGATTATGTAAATTGGTAGGACTTCCTTGTGTGGGGCCTGGAG
TTTTATCTTCTGCATTATGTATGGATAAAATATATACTAAATATGTATTAGAGAATTTTAAATTCAAACAAGCAAATTAT
GTAGTTGTAAATAAGTTTGAGTATGAGAAAAATAAAGAGGAAATAATAAAAGAAGTAGAAAAACTGCAATACGATGTATT
TATAAAACCTGCTAATAGTGGTTCATCAGTTGGAATAACAAAAGCACATAATAAAGAAGAACTTTTAAAAGGTTTAGAAG
AAGCTTTTATACATGATAAAAATGTATTAGTGGAAGAAGCTATAAATGCACGAGAGATAGAAGTAGCGGTATTAGGTAAT
GATGACCCAAAGGCTGCTGTACCTGGAGAAATAATACCAGCAAAAGAGTTTTATGATTATGAAGCAAAATATCAAAATGA
AAATTCAGAATTGCTTATACCAGCAAATATAGATAATATAAAGCAAGAAGAAATTAAAGAATTAGCTATAAAAATTTATA
AATTGTTGGGCTGTAGTGGATTAGCTAGAGTAGACTTTTTAATGGATAAAGAAAGTAATGAAGTGTATTTCAATGAAGTA
AATACATTACCTGGTTTTACAAAAATAAGTATGTATCCAAAGCTATGGGAGGCATCTGGAAAGTCCTATTCAGCCTTAAT
AGACGAGCTAATAGAATTAGCTATAAATAATAAATAA

Upstream 100 bases:

>100_bases
AACTATTTGATATATATACTTAAATTAATAGATTAAAAAGTAGTTGATTAAATTAATAAAAGATTTTAAACTATTATTTA
AGTAGTAAAATTTAATTGTT

Downstream 100 bases:

>100_bases
AATTTTGAAAGGAGAAAAACATGACAAGAGCGTTAGCAATGATCTCAGGAGGATTAGATAGTATTCTAGCAGCTAAACTA
ATGAAGGATCAAGGAATAGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLLAQQLNLYILKGEIIMKKIKIAIVFGGQSTEHEVSRNSVTSILKNINRDKYEICPIGITKEGKWFQYTGDINNIKNGQ
WEKDNKNKLENGYNVLFNKEVELVFPVLHGLYGEDGTIQGLCKLVGLPCVGPGVLSSALCMDKIYTKYVLENFKFKQANY
VVVNKFEYEKNKEEIIKEVEKLQYDVFIKPANSGSSVGITKAHNKEELLKGLEEAFIHDKNVLVEEAINAREIEVAVLGN
DDPKAAVPGEIIPAKEFYDYEAKYQNENSELLIPANIDNIKQEEIKELAIKIYKLLGCSGLARVDFLMDKESNEVYFNEV
NTLPGFTKISMYPKLWEASGKSYSALIDELIELAINNK

Sequences:

>Translated_358_residues
MLLAQQLNLYILKGEIIMKKIKIAIVFGGQSTEHEVSRNSVTSILKNINRDKYEICPIGITKEGKWFQYTGDINNIKNGQ
WEKDNKNKLENGYNVLFNKEVELVFPVLHGLYGEDGTIQGLCKLVGLPCVGPGVLSSALCMDKIYTKYVLENFKFKQANY
VVVNKFEYEKNKEEIIKEVEKLQYDVFIKPANSGSSVGITKAHNKEELLKGLEEAFIHDKNVLVEEAINAREIEVAVLGN
DDPKAAVPGEIIPAKEFYDYEAKYQNENSELLIPANIDNIKQEEIKELAIKIYKLLGCSGLARVDFLMDKESNEVYFNEV
NTLPGFTKISMYPKLWEASGKSYSALIDELIELAINNK
>Mature_358_residues
MLLAQQLNLYILKGEIIMKKIKIAIVFGGQSTEHEVSRNSVTSILKNINRDKYEICPIGITKEGKWFQYTGDINNIKNGQ
WEKDNKNKLENGYNVLFNKEVELVFPVLHGLYGEDGTIQGLCKLVGLPCVGPGVLSSALCMDKIYTKYVLENFKFKQANY
VVVNKFEYEKNKEEIIKEVEKLQYDVFIKPANSGSSVGITKAHNKEELLKGLEEAFIHDKNVLVEEAINAREIEVAVLGN
DDPKAAVPGEIIPAKEFYDYEAKYQNENSELLIPANIDNIKQEEIKELAIKIYKLLGCSGLARVDFLMDKESNEVYFNEV
NTLPGFTKISMYPKLWEASGKSYSALIDELIELAINNK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=40.7821229050279, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1786280, Length=338, Percent_Identity=33.4319526627219, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLB_CLOTE (Q898Z5)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_780997.1
- ProteinModelPortal:   Q898Z5
- SMR:   Q898Z5
- GeneID:   1059868
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00291
- NMPDR:   fig|212717.1.peg.219
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- PhylomeDB:   Q898Z5
- ProtClustDB:   PRK01966
- BioCyc:   CTET212717:CTC_00291-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40536; Mature: 40536

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAQQLNLYILKGEIIMKKIKIAIVFGGQSTEHEVSRNSVTSILKNINRDKYEICPIGI
CCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
TKEGKWFQYTGDINNIKNGQWEKDNKNKLENGYNVLFNKEVELVFPVLHGLYGEDGTIQG
CCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCHHHH
LCKLVGLPCVGPGVLSSALCMDKIYTKYVLENFKFKQANYVVVNKFEYEKNKEEIIKEVE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KLQYDVFIKPANSGSSVGITKAHNKEELLKGLEEAFIHDKNVLVEEAINAREIEVAVLGN
HCEEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECC
DDPKAAVPGEIIPAKEFYDYEAKYQNENSELLIPANIDNIKQEEIKELAIKIYKLLGCSG
CCCCCCCCCCCCCHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
LARVDFLMDKESNEVYFNEVNTLPGFTKISMYPKLWEASGKSYSALIDELIELAINNK
CHHHHEEECCCCCEEEEEECCCCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLAQQLNLYILKGEIIMKKIKIAIVFGGQSTEHEVSRNSVTSILKNINRDKYEICPIGI
CCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
TKEGKWFQYTGDINNIKNGQWEKDNKNKLENGYNVLFNKEVELVFPVLHGLYGEDGTIQG
CCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCHHHH
LCKLVGLPCVGPGVLSSALCMDKIYTKYVLENFKFKQANYVVVNKFEYEKNKEEIIKEVE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KLQYDVFIKPANSGSSVGITKAHNKEELLKGLEEAFIHDKNVLVEEAINAREIEVAVLGN
HCEEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECC
DDPKAAVPGEIIPAKEFYDYEAKYQNENSELLIPANIDNIKQEEIKELAIKIYKLLGCSG
CCCCCCCCCCCCCHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
LARVDFLMDKESNEVYFNEVNTLPGFTKISMYPKLWEASGKSYSALIDELIELAINNK
CHHHHEEECCCCCEEEEEECCCCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129