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The map label for this gene is yqiI [H]

Identifier: 28210024

GI number: 28210024

Start: 229373

End: 231163

Strand: Direct

Name: yqiI [H]

Synonym: CTC00260

Alternate gene names: 28210024

Gene position: 229373-231163 (Clockwise)

Preceding gene: 28210023

Following gene: 28210025

Centisome position: 8.19

GC content: 28.98

Gene sequence:

>1791_bases
ATGAAAAAATATAAAAATGCAATAACGTTTAGTATTATGTTTCTATTTTTATTGCTATTACCAATAGTATGTAAAGTAAA
GGCAGCGGATGATTTTAAGATTATTCCAACTAAGCAAAATGTAGAGCAAAATAAAAAATGGATTATAAAATTTAATGATA
TTTTGAAAATCAGTACAGTAAATAATGATACAATATATGTAACAGATGAAAAGGGGAAAAGGGTACAAACTAATTTAGAA
GTTGGGGCTGATAAAAAGTCTATATATGTATCACCTAAATCAAGCTATGAGTATGGAAAAACTTATTTTCTTATTGCTAA
TAAAGGAATTCAGAGGCTTTCTGGAAGAAGCATGGCTACAAATGTAAAAATGAAATTTATTATAAAAAACAATGGAGTAG
TACCACCAGTAAATGGAGATAATTTGGTTGTATGTTTGGATGCAGGGCGTGGTGGCAGCGACAAAGGTAATGTAGGTTCA
AGTGGATCATTAGAAAAAGATATAAATTTAGATGTAGCATTAAAAGCAGGATCTATTTTAGAGAATACAGGCATGAAAGT
AGTTTATACAAGAAAAGATGACAATATTAAATATGAAGAAAATGATTTAAAATCTAGGTTTCAAGTTATAGATGTAACAC
CTGTAGATGCTATAGTAAGTATACACTGTAATATAGCAGCTAATAGTGATGCTACAGGAATAGAAACTTTTTATAAAGAA
GGAGACATTAACGGGAAAAATTTAGCAGATAAAATTCAAGGGAAATTATCATATTATACTGGTATGAGAAATAGGGGTGT
AAAAACTGGGAATTTCAAAGAAATATATGCAGTTGACGAACCAATAGTAAAAATCTTTTTAGGATTTATAAATAACCCAG
AAGATGAGAAAAAATTAAATGATTCAAGCATGCAAGAGAAATTAGGAAAAGCTATAGCAGATGGAATTATAGATTATGCA
AAAGGAAGCAGTAATGGAGGGAATAATATAACTATAGCTTCAGTAGAGGATATAATAAAGAGTGTATCTGAAGGTGAAAA
ATATAATTTACCTTCAAAAGTTAATGTTAAAACCATTCAAGGTGAATCTAAAGAAGAAAGTATAATTTGGAATAGCACAA
TTGTAGATACAAGTAAAGTAGGTACATATGCATATAAAGGAAAAGTAGAGGGATATCCAAAAGAAGTTATTTTAACATTG
CTTGTTACAAATAAAGGAAATGGTAAACATATAGTTTGTATAGATCCAGGACATGGAGGTTATGATTCTGGAGCAGTAGG
TCCTACAGGGATAAAAGAAAAGGATATAGCTTTAAAAGTAGCACAAAAAACAGGTAAGATTTTAGAAAATAAAGATGTTA
AAGTAGTATATACTAGAACTAGTGATAAAGTATCATGGCCATCTTCTGAAGGATTAGATTTAAAAAAGAGAACAGAAATA
GCAAACAGTATGAATCCAAATTATTTTGTTTCCATACATTGTAATTCAGCAAATAATATACCTTCTGCAAAGGGCACAGA
GACTTACTATAGTAGAGGAAGTGTGTTAGGACAAAAATTAGCTACTAATGTTCAAAATGAATTAATTAAAAATTTAGGAA
CAATAAATAGGGGCACTAAAACAGCTAATTTTTATGTTATAAGAAACAGTAATTGCCCAGCAATATTGGCAGAATTAGAG
TTTATTAGTAATACCGAAGGAGAACAAAATTTAAACAATGAAGAATTCCAAGATAAATGTGCACAATCAATAGCAAATGG
AATTTTAAAATCTTTAGGATTATTAAATTAA

Upstream 100 bases:

>100_bases
GAACTTCTCAAAAGGCACTAATAGTTGCATTAAAAGATTGTTTTAATAAATATGAAATTTATAAACGAAAAAATAAATAT
TTTTAGAATGGGGTGTTTTC

Downstream 100 bases:

>100_bases
ATTGACAAGAAATAAACCATATAATATAATGTATTTTAATAGATTTTAATAATAATTTTATTTAAATGCTATGAAAAGGA
AGAGTACTTAAAATAACCCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKKYKNAITFSIMFLFLLLLPIVCKVKAADDFKIIPTKQNVEQNKKWIIKFNDILKISTVNNDTIYVTDEKGKRVQTNLE
VGADKKSIYVSPKSSYEYGKTYFLIANKGIQRLSGRSMATNVKMKFIIKNNGVVPPVNGDNLVVCLDAGRGGSDKGNVGS
SGSLEKDINLDVALKAGSILENTGMKVVYTRKDDNIKYEENDLKSRFQVIDVTPVDAIVSIHCNIAANSDATGIETFYKE
GDINGKNLADKIQGKLSYYTGMRNRGVKTGNFKEIYAVDEPIVKIFLGFINNPEDEKKLNDSSMQEKLGKAIADGIIDYA
KGSSNGGNNITIASVEDIIKSVSEGEKYNLPSKVNVKTIQGESKEESIIWNSTIVDTSKVGTYAYKGKVEGYPKEVILTL
LVTNKGNGKHIVCIDPGHGGYDSGAVGPTGIKEKDIALKVAQKTGKILENKDVKVVYTRTSDKVSWPSSEGLDLKKRTEI
ANSMNPNYFVSIHCNSANNIPSAKGTETYYSRGSVLGQKLATNVQNELIKNLGTINRGTKTANFYVIRNSNCPAILAELE
FISNTEGEQNLNNEEFQDKCAQSIANGILKSLGLLN

Sequences:

>Translated_596_residues
MKKYKNAITFSIMFLFLLLLPIVCKVKAADDFKIIPTKQNVEQNKKWIIKFNDILKISTVNNDTIYVTDEKGKRVQTNLE
VGADKKSIYVSPKSSYEYGKTYFLIANKGIQRLSGRSMATNVKMKFIIKNNGVVPPVNGDNLVVCLDAGRGGSDKGNVGS
SGSLEKDINLDVALKAGSILENTGMKVVYTRKDDNIKYEENDLKSRFQVIDVTPVDAIVSIHCNIAANSDATGIETFYKE
GDINGKNLADKIQGKLSYYTGMRNRGVKTGNFKEIYAVDEPIVKIFLGFINNPEDEKKLNDSSMQEKLGKAIADGIIDYA
KGSSNGGNNITIASVEDIIKSVSEGEKYNLPSKVNVKTIQGESKEESIIWNSTIVDTSKVGTYAYKGKVEGYPKEVILTL
LVTNKGNGKHIVCIDPGHGGYDSGAVGPTGIKEKDIALKVAQKTGKILENKDVKVVYTRTSDKVSWPSSEGLDLKKRTEI
ANSMNPNYFVSIHCNSANNIPSAKGTETYYSRGSVLGQKLATNVQNELIKNLGTINRGTKTANFYVIRNSNCPAILAELE
FISNTEGEQNLNNEEFQDKCAQSIANGILKSLGLLN
>Mature_596_residues
MKKYKNAITFSIMFLFLLLLPIVCKVKAADDFKIIPTKQNVEQNKKWIIKFNDILKISTVNNDTIYVTDEKGKRVQTNLE
VGADKKSIYVSPKSSYEYGKTYFLIANKGIQRLSGRSMATNVKMKFIIKNNGVVPPVNGDNLVVCLDAGRGGSDKGNVGS
SGSLEKDINLDVALKAGSILENTGMKVVYTRKDDNIKYEENDLKSRFQVIDVTPVDAIVSIHCNIAANSDATGIETFYKE
GDINGKNLADKIQGKLSYYTGMRNRGVKTGNFKEIYAVDEPIVKIFLGFINNPEDEKKLNDSSMQEKLGKAIADGIIDYA
KGSSNGGNNITIASVEDIIKSVSEGEKYNLPSKVNVKTIQGESKEESIIWNSTIVDTSKVGTYAYKGKVEGYPKEVILTL
LVTNKGNGKHIVCIDPGHGGYDSGAVGPTGIKEKDIALKVAQKTGKILENKDVKVVYTRTSDKVSWPSSEGLDLKKRTEI
ANSMNPNYFVSIHCNSANNIPSAKGTETYYSRGSVLGQKLATNVQNELIKNLGTINRGTKTANFYVIRNSNCPAILAELE
FISNTEGEQNLNNEEFQDKCAQSIANGILKSLGLLN

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=230, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790611, Length=230, Percent_Identity=26.9565217391304, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 65341; Mature: 65341

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKNAITFSIMFLFLLLLPIVCKVKAADDFKIIPTKQNVEQNKKWIIKFNDILKISTV
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEEEEECEEEEEEE
NNDTIYVTDEKGKRVQTNLEVGADKKSIYVSPKSSYEYGKTYFLIANKGIQRLSGRSMAT
CCCEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCHHHHCCCCEEE
NVKMKFIIKNNGVVPPVNGDNLVVCLDAGRGGSDKGNVGSSGSLEKDINLDVALKAGSIL
CEEEEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEE
ENTGMKVVYTRKDDNIKYEENDLKSRFQVIDVTPVDAIVSIHCNIAANSDATGIETFYKE
CCCCCEEEEEECCCCCEECCHHHCCEEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHC
GDINGKNLADKIQGKLSYYTGMRNRGVKTGNFKEIYAVDEPIVKIFLGFINNPEDEKKLN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHCC
DSSMQEKLGKAIADGIIDYAKGSSNGGNNITIASVEDIIKSVSEGEKYNLPSKVNVKTIQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEC
GESKEESIIWNSTIVDTSKVGTYAYKGKVEGYPKEVILTLLVTNKGNGKHIVCIDPGHGG
CCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCC
YDSGAVGPTGIKEKDIALKVAQKTGKILENKDVKVVYTRTSDKVSWPSSEGLDLKKRTEI
CCCCCCCCCCCCCCCCEEEEHHHHCCHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
ANSMNPNYFVSIHCNSANNIPSAKGTETYYSRGSVLGQKLATNVQNELIKNLGTINRGTK
HHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TANFYVIRNSNCPAILAELEFISNTEGEQNLNNEEFQDKCAQSIANGILKSLGLLN
EEEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKYKNAITFSIMFLFLLLLPIVCKVKAADDFKIIPTKQNVEQNKKWIIKFNDILKISTV
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEEEEECEEEEEEE
NNDTIYVTDEKGKRVQTNLEVGADKKSIYVSPKSSYEYGKTYFLIANKGIQRLSGRSMAT
CCCEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCHHHHCCCCEEE
NVKMKFIIKNNGVVPPVNGDNLVVCLDAGRGGSDKGNVGSSGSLEKDINLDVALKAGSIL
CEEEEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEE
ENTGMKVVYTRKDDNIKYEENDLKSRFQVIDVTPVDAIVSIHCNIAANSDATGIETFYKE
CCCCCEEEEEECCCCCEECCHHHCCEEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHC
GDINGKNLADKIQGKLSYYTGMRNRGVKTGNFKEIYAVDEPIVKIFLGFINNPEDEKKLN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHCC
DSSMQEKLGKAIADGIIDYAKGSSNGGNNITIASVEDIIKSVSEGEKYNLPSKVNVKTIQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEC
GESKEESIIWNSTIVDTSKVGTYAYKGKVEGYPKEVILTLLVTNKGNGKHIVCIDPGHGG
CCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCC
YDSGAVGPTGIKEKDIALKVAQKTGKILENKDVKVVYTRTSDKVSWPSSEGLDLKKRTEI
CCCCCCCCCCCCCCCCEEEEHHHHCCHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
ANSMNPNYFVSIHCNSANNIPSAKGTETYYSRGSVLGQKLATNVQNELIKNLGTINRGTK
HHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TANFYVIRNSNCPAILAELEFISNTEGEQNLNNEEFQDKCAQSIANGILKSLGLLN
EEEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]