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The map label for this gene is murI2 [H]

Identifier: 28210023

GI number: 28210023

Start: 228063

End: 228863

Strand: Direct

Name: murI2 [H]

Synonym: CTC00259

Alternate gene names: 28210023

Gene position: 228063-228863 (Clockwise)

Preceding gene: 28210022

Following gene: 28210024

Centisome position: 8.15

GC content: 28.09

Gene sequence:

>801_bases
TTGAGGGGGTTGATGCTTATGATTACAAAGGACAGTGTTATAGGAGTTTTAGATTCAGGAGTAGGAGGACTTACAACAGT
AAAGGAACTTCAAGAATTATTACCGAATGAAAATATATACTACTTTGGTGATAATAAAAATGTTCCTTATGGAAATAAGA
CACAGGAAGAAATTATACAGTTTACTAGAAATATGATAGAATTTTTAGTATCGCAAAATGTTAAAGTAATAGCTGTTGCT
TGTAATACCATTTCAACTATTATAGACAAGATAAGTGGAGATTATGATGTTAAGATAATAAAAGTAATAGATCCTATAGT
AAACAATATAAAGAATATTCAGAAGAAACATAAGGTTGGGTTAATTGCAACAAGCTTTACCATAAGTACCAAATACTATG
ATGAAATGTTAGGAGATGTAAAGGTTATTGGGAAGGGATCACCAGAACTTGCAGCAATAGTAGATAGTGGAGAAATAAAT
GAAAAGGAAGTTAAAGATATTATAAAGGTTCATATAGAAGATATTAAATCAAGAGAGAATGTAGATACAATAATATTGGG
ATGTACTCACTACCCTATAATTAAGCACTTTTTTGATGAGTGTTATCCAGATATAAACTTTTTAAATCCAGCACATCAAC
ATGTTATTGCTGTAAAAGAATATTTAGAGGAAATGAATTTACTTAGAGAAGATAATAATAATAAATTTTTTAAGATTTTT
ACTACGGGAGATAAAGAAAAATATAGAATTATAATGAACATGCTTAAATTAGAAGAACCTGATGAAATAATATTAAAGTA
G

Upstream 100 bases:

>100_bases
TTCTTATGTTTGTATGTAAGTTAGCGAAAGCTGAAACTTATATACATAAATGTAAAGAAGCCGGATTTTTGTTGTAAAAA
CAATAATCTGGCTTTATTTT

Downstream 100 bases:

>100_bases
AAATCTTTTAAAAAGGAATTACTTCACTTTAGAAATTAATTTTCAAAGTGAAATAATTCCTTTTTTGTTTCAATAAAGTA
TAAATTAAAGTGAAATTATT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRGLMLMITKDSVIGVLDSGVGGLTTVKELQELLPNENIYYFGDNKNVPYGNKTQEEIIQFTRNMIEFLVSQNVKVIAVA
CNTISTIIDKISGDYDVKIIKVIDPIVNNIKNIQKKHKVGLIATSFTISTKYYDEMLGDVKVIGKGSPELAAIVDSGEIN
EKEVKDIIKVHIEDIKSRENVDTIILGCTHYPIIKHFFDECYPDINFLNPAHQHVIAVKEYLEEMNLLREDNNNKFFKIF
TTGDKEKYRIIMNMLKLEEPDEIILK

Sequences:

>Translated_266_residues
MRGLMLMITKDSVIGVLDSGVGGLTTVKELQELLPNENIYYFGDNKNVPYGNKTQEEIIQFTRNMIEFLVSQNVKVIAVA
CNTISTIIDKISGDYDVKIIKVIDPIVNNIKNIQKKHKVGLIATSFTISTKYYDEMLGDVKVIGKGSPELAAIVDSGEIN
EKEVKDIIKVHIEDIKSRENVDTIILGCTHYPIIKHFFDECYPDINFLNPAHQHVIAVKEYLEEMNLLREDNNNKFFKIF
TTGDKEKYRIIMNMLKLEEPDEIILK
>Mature_266_residues
MRGLMLMITKDSVIGVLDSGVGGLTTVKELQELLPNENIYYFGDNKNVPYGNKTQEEIIQFTRNMIEFLVSQNVKVIAVA
CNTISTIIDKISGDYDVKIIKVIDPIVNNIKNIQKKHKVGLIATSFTISTKYYDEMLGDVKVIGKGSPELAAIVDSGEIN
EKEVKDIIKVHIEDIKSRENVDTIILGCTHYPIIKHFFDECYPDINFLNPAHQHVIAVKEYLEEMNLLREDNNNKFFKIF
TTGDKEKYRIIMNMLKLEEPDEIILK

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=184, Percent_Identity=29.3478260869565, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 30344; Mature: 30344

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLMLMITKDSVIGVLDSGVGGLTTVKELQELLPNENIYYFGDNKNVPYGNKTQEEIIQ
CCCEEEEEECCCEEEHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
FTRNMIEFLVSQNVKVIAVACNTISTIIDKISGDYDVKIIKVIDPIVNNIKNIQKKHKVG
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHE
LIATSFTISTKYYDEMLGDVKVIGKGSPELAAIVDSGEINEKEVKDIIKVHIEDIKSREN
EEEEEEEEHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
VDTIILGCTHYPIIKHFFDECYPDINFLNPAHQHVIAVKEYLEEMNLLREDNNNKFFKIF
CCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TTGDKEKYRIIMNMLKLEEPDEIILK
ECCCCHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MRGLMLMITKDSVIGVLDSGVGGLTTVKELQELLPNENIYYFGDNKNVPYGNKTQEEIIQ
CCCEEEEEECCCEEEHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
FTRNMIEFLVSQNVKVIAVACNTISTIIDKISGDYDVKIIKVIDPIVNNIKNIQKKHKVG
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHE
LIATSFTISTKYYDEMLGDVKVIGKGSPELAAIVDSGEINEKEVKDIIKVHIEDIKSREN
EEEEEEEEHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
VDTIILGCTHYPIIKHFFDECYPDINFLNPAHQHVIAVKEYLEEMNLLREDNNNKFFKIF
CCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TTGDKEKYRIIMNMLKLEEPDEIILK
ECCCCHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]