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The map label for this gene is ppsA [H]

Identifier: 77747646

GI number: 77747646

Start: 640403

End: 642778

Strand: Reverse

Name: ppsA [H]

Synonym: PD0524

Alternate gene names: 77747646

Gene position: 642778-640403 (Counterclockwise)

Preceding gene: 28198438

Following gene: 28198433

Centisome position: 25.51

GC content: 53.66

Gene sequence:

>2376_bases
TTGAACGAAAATATCCTTTGGTTACATGAACTGCGCTTGACCGATCTGGCCCGCGTCGGTGGTAAAAATTCCTCACTCGG
CGAAATGATCGGCAATCTCACTGCCCTTGGAGTCTCTGTTCCAGGTGGCTATGCCACCACCGCAGCAGCATTTAAAGCCT
TTATCGCTTACAACAACCTGTCCAAACGGATCTACGACAAACTAGCCACCCTTGATGTTGAGGACGTCAAAGCACTGACA
GCTGCCGGCAAAGAAATCCGCGACTGGGTCATTGACGCTCCATTACAACCAGAACTGGAGAAGGATATTCGTGACGCCTA
CGACACGTTGTCAAAAGACAATAGCGGCAACGACGTCGCCGTAGCGGTGCGCTCTTCAGCGACCGCGGAAGATTTACCAG
ACGCCTCATTCGCCGGCCAACAGGAAACCTTTCTTAATGTGATCGGCGCCGACCAGGTCGTACACAAAATTAAAGAAGTA
TTCGCAAGTCTTTATAACGATCGTGCAATTGCCTACCGCGTCCATCATGGCTTCAAGCACGAGGACGTATTCCTCTCCGC
CGGAATACAACTGATGGTTCGCTCGGATATCAGTGCCTCCGGCGTGCTGTTCACTCTAGACACCGAGTCAGGCTTCCGCG
AGGTGGTGTTCGTCACCGCCAGCTTCGGCCTTGGTGAAATGGTCGTACAGGGCGCCGTCAACCCAGACGAATTCTATGTC
TATAAAAAGACGCTCAAAGCTGGCAAACCTGCCATCCTGCGCCGCACACTCGGCACCAAACTGGTGCGCATGGTCTATTC
AGACGCCCCAGGAGAGCGCGTCAAGATCGAAGATACCCCAACTGAATTACGTCACTGCTTCGCCATCAGCGACGCTGACG
TACAAGAGCTGGCGAGACAAGCACTGATTATTGAGCAGCACTACGGCCGCCCGATGGACATTGAATGGGCTAAGGATGGG
ATCAGCGGCAAATTATTTATTGTGCAGGCACGTCCGGAGACAGTGAAATCACGCAGTCACACCACCCAGATTGAACGCTT
TGCACTCCAACAACCCGGCCAGATACTGACAGAGGGACGTGCCATCGGCCAGAAGATCGGCAGCGGCACAGCCCGTGTGG
TGAACTCACTTGAGGACATGCACCACATGCAACCCGGCGATGTCCTGGTTGCAGACATGACCGACCCAGACTGGGAGCCA
GTCATGAAACGCGCCAGCGCGATTGTCACCAATCGCGGCGGACGTACCTGCCACGCCGCCATTATCGCCCGTGAGCTGGG
AGTACCCGCAGTGGTTGGCACCGGCAATGCGCTATCAATCATTCAAGACGGCCAACCCATCACGGTAAGCTGCGCCGAAG
GTGACACCGGCTATATCTACGCCGGGAAACTACCGTTCGAACGCACCACAACAGACCTCGGCCACATGCCACCAGCGCCG
CTAAAAATCATGATGAATGTTGCCAATCCGGAACGTGCGTTCGACTTTGGGCAACTCCCGAATGCCGGTATCGGCTTGGC
ACGCCTGGAAATGATCATCGCCGCACACATCGGCGTGCACCCAAAAGCACTTCTGGAATACCACAAACAAGATGCTGACA
CGAAACAGAAGATTGACGCCAAGATTGCCGGTTATCCTGATCCAGTCAGCTTCTACGTCAACCGCCTCGCCGAAGGTATT
GCGACCCTGACCGCCTCAGTCGCACCCCATACGGTCATCGTGCGCCTGTCGGACTTCAAATCCAACGAATACGCACACCT
GATCGGTGGCCAGCACTATGAGCCACAAGAAGAAAACCCGATGCTCGGCTTCCGCGGTGCCAGCCGTTACGTCGACCCTT
CATTCAGCGATGCCTTCGCATTAGAGTGCAAAGCAGTACTAAAAGTCCGTAACGAGATGGGTCTAGATAACCTCTGGGTG
ATGATTCCATTTGTACGCACTTTGGAAGAAGGTCGCAAAGTGATCGACGTACTGCGACAAAATGGCTTAAAACAGGGAGA
AAATGGTTTAAAAGTGATCATGATGTGCGAAGTGCCCTCCAACGCACTGCTGGCTGATGAATTCCTGGATATCTTCGACG
GCTTCTCAATCGGCTCCAACGATCTCACCCAACTTTCCCTAGGTCTGGACCGCGACTCCTCCATCGTCGCCCCTCTATTT
GACGAGCGCAATCCAGCCGTCAAAAAACTACTGTCAATGGCAATAAAAGCGGCACGTTCCAAGGGAAAATACATTGGAAT
CTGCGGCCAAGGCCCATCCGATCATCCAGACTTGGCCGAATGGCTGATGCAAGAAGGCATAGAATCCCTGTCACTGAACC
CAGATACCGTCGTCGATACTTGGCTACGCTTGGCAAAACTAAAAACGGTGCACTAA

Upstream 100 bases:

>100_bases
TTGTGCAGTCTTGCCTATGCACTGCATCATACGACCACTTTAATACGGACGCGGCCATACAGCCCTCCTCCAGGCGATGG
CCCTAAACGGAGCATCGTAC

Downstream 100 bases:

>100_bases
ATGGACTGGACTAAATTACTATCAAACATCGTATCGCCTGCAACAGTGACGACGTTCCACGGCAGATTCAACTGGACACG
GATCGACTGGATCCACATCC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLDVEDVKALT
AAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEV
FASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAVNPDEFYV
YKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPMDIEWAKDG
ISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDLGHMPPAP
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFYVNRLAEGI
ATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWV
MIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDSSIVAPLF
DERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH

Sequences:

>Translated_791_residues
MNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLDVEDVKALT
AAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEV
FASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAVNPDEFYV
YKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPMDIEWAKDG
ISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDLGHMPPAP
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFYVNRLAEGI
ATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWV
MIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDSSIVAPLF
DERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH
>Mature_791_residues
MNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLDVEDVKALT
AAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEV
FASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAVNPDEFYV
YKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPMDIEWAKDG
ISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEP
VMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDLGHMPPAP
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFYVNRLAEGI
ATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWV
MIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDSSIVAPLF
DERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=786, Percent_Identity=64.1221374045801, Blast_Score=1071, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=163, Percent_Identity=36.8098159509202, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788756, Length=396, Percent_Identity=25.7575757575758, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI48994992, Length=162, Percent_Identity=32.7160493827161, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789193, Length=403, Percent_Identity=23.5732009925558, Blast_Score=86, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=397, Percent_Identity=25.1889168765743, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86529; Mature: 86529

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNL
CCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHCHHEEEEECCCCHHHHHHHHHHHHHHHHH
SKRIYDKLATLDVEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVA
HHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
VAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEVFASLYNDRAIAYRVHHGFKH
EEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
EDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAVNPDEFYV
CCEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHCCCCCHHEEE
YKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQ
EHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHHHH
ALIIEQHYGRPMDIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGR
HHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCHHHHHHH
AIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEPVMKRASAIVTNRGGRTCHAA
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDLGHMPPAP
HHHHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCCCCC
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDA
CEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCH
KIAGYPDPVSFYVNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENP
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCC
MLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWVMIPFVRTLEEGRKVIDVLRQ
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDSSIVAPLF
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEECCCC
DERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDT
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCHHHHHH
WLRLAKLKTVH
HHHHHHHHCCC
>Mature Secondary Structure
MNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNL
CCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHCHHEEEEECCCCHHHHHHHHHHHHHHHHH
SKRIYDKLATLDVEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVA
HHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
VAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEVFASLYNDRAIAYRVHHGFKH
EEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
EDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAVNPDEFYV
CCEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHCCCCCHHEEE
YKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQ
EHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHHHH
ALIIEQHYGRPMDIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGR
HHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCHHHHHHH
AIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEPVMKRASAIVTNRGGRTCHAA
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDLGHMPPAP
HHHHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCCCCC
LKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDA
CEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHCH
KIAGYPDPVSFYVNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENP
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCC
MLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWVMIPFVRTLEEGRKVIDVLRQ
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDSSIVAPLF
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEECCCC
DERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDT
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCCCCHHHHHH
WLRLAKLKTVH
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]