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The map label for this gene is fumB [H]

Identifier: 28198848

GI number: 28198848

Start: 1145975

End: 1147492

Strand: Reverse

Name: fumB [H]

Synonym: PD0947

Alternate gene names: 28198848

Gene position: 1147492-1145975 (Counterclockwise)

Preceding gene: 77747671

Following gene: 28198846

Centisome position: 45.54

GC content: 53.03

Gene sequence:

>1518_bases
GTGTCATTGATCAAACAAGAAGACTTTATCCAGTCCATCGCTGATGCGCTGCAATACATCAGTTACTACCATCCGGTCGA
TTACATCAAGAATCTGGCTTTGGCGTATGGGTGCGAGAAATCGCCCGCCGCTAAAGATGCAATGGCGCAGATTTTGATTA
ACTCACGTATGTGTGCTGAGGGGCGCCGCCCGATTTGCCAAGATACAGGGATTGTCACGGTGTTCCTTAGCATCGGGATG
GATGTACGTTGGGATGACGTCACGATGGATGTGGAAGAGATGGTGAATGAGGGGGTTCGCCGTGCCTATCGTCATCCGGA
TAACACGCTACGTGCGTCTGTGCTTGCTGATCCTGCCGGCAAGCGCTGCAATACGAGGGACAATACGCCAGCAGTCGTTA
ACGTCAAGGTTGTTTCTGGGGGCCATGTTGAAGTGATTGTTGCTGCCAAGGGAGGGGGATCTGAGGCCAAGAGCAAATTT
GCAATGCTAAATCCATCGGATTCCATCGTTGATTGGGTTCTGGACACTGTGCCAACCATGGGGGCTGGCTGGTGCCCACC
AGGGATGCTTGGGATTGGTATCGGTGGTACGGCTGAGAAAGCGATGCTGCTCGCTAAGGAAGCGTTGATGGAACCGATTG
ACATCCAGGATTTGATGGTACGCGGCCCTTCCAATCGTGCTGAAGAATTACGCTTGGAGTTGTACGAGAAGGTCAATGCT
TTAGGGATCGGTGCTCAGGGGGTGGGCGGTTTGACGACGGTATTAGATATCAAGGTCAAAGATTATCCAACGCATGCTGC
CAATTTGCCGGTAGCGATGATCCCTAATTGTGCGTCCACACGCCATGCGCATTTCACATTGGACGGTAGCGGTCCTGTTG
TTCTGGAACCGCCGTCATTAGAGGATTGGCCTAAGTTGAGCTATCTCCCGGAGTCTGCACGGCAGGTGGATTTGAATACG
ATCACCAGGGATGAGGTGGCGCAGTTCAAGCCAGGTGAGGTGTTGCTACTCAGCGGCAAATTGTTGACGGGACGAGATGC
TGCGCATAAGCGCATCGTTGAGATGCTCAACAAGGGGGAGACATTACCAGTTGATTTCACGAATCGCTTTATCTACTACG
TGGGTCCAGTGGATCCTGTGCGTGATGAAGTCGTCGGTCCTGCGGGTCCAACCACAGCAACGCGTATGGATAAGTTTACG
CGTCAGATGCTGGAGCAGGGCGGGTTGCTCGGTATGGTGGGCAAGGCCGAGCGTGGTCCTACTGCCATCGCTGCAATCCG
TGACAACAAAGCCGTGTATCTGATGGCGGTGGGAGGGGCGGCTTATCTTGTCTCCAAGGCGATCAAAACGAGCCGGATAC
TGGCTTTTGAAGACCTTGGCATGGAGGCAATTTATGAGTTTGAAGTCAAGGAGATGCCTGTAACAGTGGCGGTTGATTGC
AACGGTGAGTCGATACATACAACTGGGCCGCGTGAGTGGCGAAGCCGGATCGGGAAAATTCCGGTTGTTGTGGAGTAG

Upstream 100 bases:

>100_bases
GCGGTTTCAGTGGGTGTGGATCGCAATGTTTATCCTATGGCTGATGTATTCAGGGGGATTACAATGGCAACGTTTTTTAG
TCAGCCATAAGGGCCAGCCG

Downstream 100 bases:

>100_bases
GAATGGCGCAACGCTTGGAAGCACTGGAACAGTGGGTTCATTGTCAGGTGGTGCGTTCTCTACGGTGTTGCATCGGGTTG
GTGCGTCATGTGGGCGACAG

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINSRMCAEGRRPICQDTGIVTVFLSIGM
DVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSEAKSKF
AMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRLELYEKVNA
LGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVLEPPSLEDWPKLSYLPESARQVDLNT
ITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFT
RQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILAFEDLGMEAIYEFEVKEMPVTVAVDC
NGESIHTTGPREWRSRIGKIPVVVE

Sequences:

>Translated_505_residues
MSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINSRMCAEGRRPICQDTGIVTVFLSIGM
DVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSEAKSKF
AMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRLELYEKVNA
LGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVLEPPSLEDWPKLSYLPESARQVDLNT
ITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFT
RQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILAFEDLGMEAIYEFEVKEMPVTVAVDC
NGESIHTTGPREWRSRIGKIPVVVE
>Mature_504_residues
SLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINSRMCAEGRRPICQDTGIVTVFLSIGMD
VRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSEAKSKFA
MLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRLELYEKVNAL
GIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVLEPPSLEDWPKLSYLPESARQVDLNTI
TRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTR
QMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILAFEDLGMEAIYEFEVKEMPVTVAVDCN
GESIHTTGPREWRSRIGKIPVVVE

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=474, Percent_Identity=31.8565400843882, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787897, Length=469, Percent_Identity=32.409381663113, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789442, Length=285, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789443, Length=177, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54778; Mature: 54647

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHC
GRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAG
CCCCCCCCCCCEEEEECCCCCEEECCCEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
KRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLDTVPTM
CCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHCCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRLELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHH
LGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVLEPPSL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEECCCCC
EDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGE
CCCCCCCCCCCCHHEEECHHCCHHHHHHCCCCCEEEEECCEECCCHHHHHHHHHHHHCCC
TLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQGGLLGMVGKAERGP
CCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILAFEDLGMEAIYEFEVKEMPVTVAVDC
EEEEEEECCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCCEEEEEEHHHCCEEEEEEC
NGESIHTTGPREWRSRIGKIPVVVE
CCCEEECCCCHHHHHHHCCCCEEEC
>Mature Secondary Structure 
SLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHC
GRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAG
CCCCCCCCCCCEEEEECCCCCEEECCCEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
KRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLDTVPTM
CCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHCCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRLELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHH
LGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVLEPPSL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEECCCCC
EDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGE
CCCCCCCCCCCCHHEEECHHCCHHHHHHCCCCCEEEEECCEECCCHHHHHHHHHHHHCCC
TLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQGGLLGMVGKAERGP
CCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILAFEDLGMEAIYEFEVKEMPVTVAVDC
EEEEEEECCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCCCEEEEEEHHHCCEEEEEEC
NGESIHTTGPREWRSRIGKIPVVVE
CCCEEECCCCHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]