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The map label for this gene is exsB

Identifier: 28198846

GI number: 28198846

Start: 1142680

End: 1143372

Strand: Reverse

Name: exsB

Synonym: PD0945

Alternate gene names: 28198846

Gene position: 1143372-1142680 (Counterclockwise)

Preceding gene: 28198848

Following gene: 28198844

Centisome position: 45.38

GC content: 53.54

Gene sequence:

>693_bases
ATGAAGAAAGCTGTTATCTTGTTATCCGGAGGTATGGACTCTGCTGTGGTCACCGCCATCGCCCAATCGCAGGGATTTAT
GGTGCACGCTTTAAGCATACGTTACGGTCAGCGCCATACTTCCGAGTTGGACGCAGCCGTACGGATCGCCAGGGCGCTAA
ACGTGGTTGCACATAAAGTTGTGGACGTGGATCTACGCAGCATCGGTGGTTCGGCACTGACTGACGACATCGAAATACCA
GACGCAGGTGGCGAGGGTATTCCCGTGACCTATGTGCCAGCACGTAATACCATCATGTTGTCACTCGCATTAGGTTGGGC
TGAAGTCATCGGTGCGGCCGATATATTCTGCGGTGTTAACGCCGTTGATTATTCAGGTTATCCCGATTGCCGCCCGCAGT
TCATCACGGCCTTCGAAACGCTGGCTAATCTGGCAACCAAAGTCGGCGTTGAGGGGACTCAGTTACACGTACATGCGCCA
TTACAGTTTCTCAGTAAAGCGGAGATTGTTCATGAAGGTCTGCTGCATGGCGTGGACTTTGGATTGACCGTGTCTTGTTA
TCGTGCAGATGTCGACGGGCGCGCTTGCGGGCGCTGCGACGCTTGCAAACTACGTGTGGCCGGATTCGCTGATGCTGGTG
TTGTCGACCCGACTCGCTATATGGAGTTACCCTGCTCGTTACTCTTGCTGTAA

Upstream 100 bases:

>100_bases
TATTGTCATAGCGACGTTTGCTTATGATTGCTGTTAGATGTTTCTATCCCTCTTGTTTCTATCCCCCTGATTTTTCAAAC
TTTGACTCTAAAGTTTCTAT

Downstream 100 bases:

>100_bases
AATAGACAGCTGCATTTCAATGTGCGGAAGATTGTGGTCTTGGGCCGTTAGCTCAGTCGGTAGAGCATCGGACTTTTAAT
CCGCTGGTCGCTGGTTCGAT

Product: transcriptional regulator

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKVVDVDLRSIGGSALTDDIEIP
DAGGEGIPVTYVPARNTIMLSLALGWAEVIGAADIFCGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAP
LQFLSKAEIVHEGLLHGVDFGLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMELPCSLLLL

Sequences:

>Translated_230_residues
MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKVVDVDLRSIGGSALTDDIEIP
DAGGEGIPVTYVPARNTIMLSLALGWAEVIGAADIFCGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAP
LQFLSKAEIVHEGLLHGVDFGLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMELPCSLLLL
>Mature_230_residues
MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKVVDVDLRSIGGSALTDDIEIP
DAGGEGIPVTYVPARNTIMLSLALGWAEVIGAADIFCGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAP
LQFLSKAEIVHEGLLHGVDFGLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMELPCSLLLL

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=225, Percent_Identity=38.6666666666667, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_XYLF2 (B2I4Y4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829703.1
- ProteinModelPortal:   B2I4Y4
- SMR:   B2I4Y4
- GeneID:   6203628
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0998
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- ProtClustDB:   CLSK446376
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24339; Mature: 24339

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKV
CCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VDVDLRSIGGSALTDDIEIPDAGGEGIPVTYVPARNTIMLSLALGWAEVIGAADIFCGVN
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCE
AVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAPLQFLSKAEIVHEGLLHGVDF
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCC
GLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMELPCSLLLL
CEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEECC
>Mature Secondary Structure
MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKV
CCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VDVDLRSIGGSALTDDIEIPDAGGEGIPVTYVPARNTIMLSLALGWAEVIGAADIFCGVN
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCE
AVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAPLQFLSKAEIVHEGLLHGVDF
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCC
GLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMELPCSLLLL
CEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA