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The map label for this gene is dnaA [H]

Identifier: 62185088

GI number: 62185088

Start: 529040

End: 530422

Strand: Direct

Name: dnaA [H]

Synonym: CAB459

Alternate gene names: 62185088

Gene position: 529040-530422 (Clockwise)

Preceding gene: 62185087

Following gene: 62185090

Centisome position: 46.23

GC content: 38.39

Gene sequence:

>1383_bases
ATGCGGGCGTGGGAAGACTTTCTTTTGCTACAAGAAAAAGAAATCGGTACGAGTACTGTAGACAAATGGTTAAGGTCGTT
AAAAGTCCTATGCTTTGATGCGTGTAATTTGTATCTTGAAGCCAAAGATTCTTTTCAAGTGACTTGGTTTGAGGAGCATA
TACGCCACAAGGTAAAAACAAGTTTAGTGAATAATAACGGGAAGTTAATTCGTGTTCACGTTACTTCCCTCGATAAAACT
ACTCCTTTCTATAAAGAAAAGCAGATGCAGCAGGAAAAAACTGCCTATTTTACTATGCAGTATGGCAATGTCAATCCTGA
AATGACTTTTGCTAATTTTCTTGTGACACCTGAAAATGACTTGCCGTTTCGTATTTTACAGGAGTTTACAAAACCTTCAG
AAGATGCTACAGGCTTTCCTTTTAACCCCATTTATCTTTTTGGACCAGAGGGGTCGGGGAAAACACATTTAATGCAAGCT
GCTGTAAATGTTCTTCGGGAATCTGGGGGTAAAATTCTTTACGTTGCTTCAGATTTATTTACTGAACATTTAGTTTCTGC
CATACGTTCTGGAGAAATGCAGAGATTTCGTTCTTTTTACCGTAATGTCGATGCTTTATTTATAGAGGACATAGAAGTAT
TCTCGGGTAAAGGAGCTACTCAGGAAGAGTTTTTCCATACTTTTAATTCCTTGCAAACAGAAGGTAAGTTGATTGTTATC
TCATCGGCATATGCTCCTGCAGACTTAAAAGCAATGGAAGAACGCTTAATTAGTCGTTTTGAATGGGGAGTCGCTGTTCT
TATCCATCCTTTAACGAAAGAGGGATTAAGAAGTTTTCTCATGCGACAAACAGAGCAATTATCTATCCGCATAGAAGACT
CAGCTTTGGACTTCTTAATTCACGCACTATCTTCAAACATGAAAGCGCTACTACATGCGTTAACTTTGCTTTCAAAGCGT
GTTGCTTATAAAAAACTAACTCAGCAACTGCTCTATGAAGATGATATACAATCTCTCTTACACGATGTATTAGAAGCTGC
GGAAAGTGTGCGTTTAACTCCTTCAGGAATTGTGCATGCTGTGGCTAAGTATTATGGAGTGTCTCCAGAGAGTATTTTAG
GGCGTTCACAATCTCGAGAGTATGTCTTGCCTAGACAAGTCGCTATGTACTTATGTCGGCAGAAATTGTCTTTATCCTAC
GTAAGGATAGGGGATGTATTTTCTAGAGATCATTCAACAGTAATTTCATCAATACGTGCTATTTCTCAAAAAGTAGAAGA
AGGTGGGCACGACATTAGTATTGCTACGCAAGACTTAATGAAGTATTTAACTTCAGCGTATAAGAGTCTTGAGTTTTTCC
CTGAAGAAGAAATCCCTTGTTAG

Upstream 100 bases:

>100_bases
CGAAATCACTGTTAATAAGAAAAAGCAAAGGTAACAACTGAAAAAAAAGCTTTTTTATACTAAAAAAGATATAAGAAAAC
TATTTAGATGAGATAGTTTT

Downstream 100 bases:

>100_bases
CCTACACTAACTTTTTCCTCAGAGCATAGTGGATAATCAAACAGGCTATAGACACTATTGTCAAAGCAATCTGTAAAATG
AGTGCTACTAAAATTGTAGG

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRAWEDFLLLQEKEIGTSTVDKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKTSLVNNNGKLIRVHVTSLDKT
TPFYKEKQMQQEKTAYFTMQYGNVNPEMTFANFLVTPENDLPFRILQEFTKPSEDATGFPFNPIYLFGPEGSGKTHLMQA
AVNVLRESGGKILYVASDLFTEHLVSAIRSGEMQRFRSFYRNVDALFIEDIEVFSGKGATQEEFFHTFNSLQTEGKLIVI
SSAYAPADLKAMEERLISRFEWGVAVLIHPLTKEGLRSFLMRQTEQLSIRIEDSALDFLIHALSSNMKALLHALTLLSKR
VAYKKLTQQLLYEDDIQSLLHDVLEAAESVRLTPSGIVHAVAKYYGVSPESILGRSQSREYVLPRQVAMYLCRQKLSLSY
VRIGDVFSRDHSTVISSIRAISQKVEEGGHDISIATQDLMKYLTSAYKSLEFFPEEEIPC

Sequences:

>Translated_460_residues
MRAWEDFLLLQEKEIGTSTVDKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKTSLVNNNGKLIRVHVTSLDKT
TPFYKEKQMQQEKTAYFTMQYGNVNPEMTFANFLVTPENDLPFRILQEFTKPSEDATGFPFNPIYLFGPEGSGKTHLMQA
AVNVLRESGGKILYVASDLFTEHLVSAIRSGEMQRFRSFYRNVDALFIEDIEVFSGKGATQEEFFHTFNSLQTEGKLIVI
SSAYAPADLKAMEERLISRFEWGVAVLIHPLTKEGLRSFLMRQTEQLSIRIEDSALDFLIHALSSNMKALLHALTLLSKR
VAYKKLTQQLLYEDDIQSLLHDVLEAAESVRLTPSGIVHAVAKYYGVSPESILGRSQSREYVLPRQVAMYLCRQKLSLSY
VRIGDVFSRDHSTVISSIRAISQKVEEGGHDISIATQDLMKYLTSAYKSLEFFPEEEIPC
>Mature_460_residues
MRAWEDFLLLQEKEIGTSTVDKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKTSLVNNNGKLIRVHVTSLDKT
TPFYKEKQMQQEKTAYFTMQYGNVNPEMTFANFLVTPENDLPFRILQEFTKPSEDATGFPFNPIYLFGPEGSGKTHLMQA
AVNVLRESGGKILYVASDLFTEHLVSAIRSGEMQRFRSFYRNVDALFIEDIEVFSGKGATQEEFFHTFNSLQTEGKLIVI
SSAYAPADLKAMEERLISRFEWGVAVLIHPLTKEGLRSFLMRQTEQLSIRIEDSALDFLIHALSSNMKALLHALTLLSKR
VAYKKLTQQLLYEDDIQSLLHDVLEAAESVRLTPSGIVHAVAKYYGVSPESILGRSQSREYVLPRQVAMYLCRQKLSLSY
VRIGDVFSRDHSTVISSIRAISQKVEEGGHDISIATQDLMKYLTSAYKSLEFFPEEEIPC

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=472, Percent_Identity=30.5084745762712, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52554; Mature: 52554

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAWEDFLLLQEKEIGTSTVDKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKT
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEHHHHHHHHHHHH
SLVNNNGKLIRVHVTSLDKTTPFYKEKQMQQEKTAYFTMQYGNVNPEMTFANFLVTPEND
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEECCCCC
LPFRILQEFTKPSEDATGFPFNPIYLFGPEGSGKTHLMQAAVNVLRESGGKILYVASDLF
CCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHH
TEHLVSAIRSGEMQRFRSFYRNVDALFIEDIEVFSGKGATQEEFFHTFNSLQTEGKLIVI
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEE
SSAYAPADLKAMEERLISRFEWGVAVLIHPLTKEGLRSFLMRQTEQLSIRIEDSALDFLI
ECCCCCHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHCCCHHEEEEECHHHHHHHH
HALSSNMKALLHALTLLSKRVAYKKLTQQLLYEDDIQSLLHDVLEAAESVRLTPSGIVHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VAKYYGVSPESILGRSQSREYVLPRQVAMYLCRQKLSLSYVRIGDVFSRDHSTVISSIRA
HHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHCHHHHHHHHHHH
ISQKVEEGGHDISIATQDLMKYLTSAYKSLEFFPEEEIPC
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRAWEDFLLLQEKEIGTSTVDKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKT
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEHHHHHHHHHHHH
SLVNNNGKLIRVHVTSLDKTTPFYKEKQMQQEKTAYFTMQYGNVNPEMTFANFLVTPEND
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEECCCCC
LPFRILQEFTKPSEDATGFPFNPIYLFGPEGSGKTHLMQAAVNVLRESGGKILYVASDLF
CCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHH
TEHLVSAIRSGEMQRFRSFYRNVDALFIEDIEVFSGKGATQEEFFHTFNSLQTEGKLIVI
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEE
SSAYAPADLKAMEERLISRFEWGVAVLIHPLTKEGLRSFLMRQTEQLSIRIEDSALDFLI
ECCCCCHHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHCCCHHEEEEECHHHHHHHH
HALSSNMKALLHALTLLSKRVAYKKLTQQLLYEDDIQSLLHDVLEAAESVRLTPSGIVHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VAKYYGVSPESILGRSQSREYVLPRQVAMYLCRQKLSLSYVRIGDVFSRDHSTVISSIRA
HHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHCHHHHHHHHHHH
ISQKVEEGGHDISIATQDLMKYLTSAYKSLEFFPEEEIPC
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]