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The map label for this gene is smpB

Identifier: 62185061

GI number: 62185061

Start: 498730

End: 499182

Strand: Direct

Name: smpB

Synonym: CAB432

Alternate gene names: 62185061

Gene position: 498730-499182 (Clockwise)

Preceding gene: 62185057

Following gene: 62185065

Centisome position: 43.58

GC content: 40.18

Gene sequence:

>453_bases
ATGGCCAGTAAGGAAATTGTTTCTAACCGCAAAGCCTTACATAATTACGAGGTTTTGGAATCTTTAGAAGCTGGGATTGT
TCTTACCGGAACGGAAATCAAGTCGTTGCGTGATCACGGTGGAAATCTTGGTGATGCCTATGTGGCTATCTCTAAAGGTG
AGGCGTGGTTATTGAATGCCAGCATCGCTCCATACCGTTTTGGGAATATCTATAATCATGAGGAACGAAGAAAGCGTAAA
CTTCTTCTTCATAGATACGAGATTCACAAATTAGAAGTGAAGGTCGCTCAAAAAGGAGTAACAATTATTCCTCTTGGGAT
GTTTCTTTCTCGTGGTTATGTAAAAGTTCGTTTGGGTTGTTGCCGTGGTAAAAAATCTCATGATAAGCGACAAACAATCA
TTGCAAGAGAGAAAGAAAGAGAAATAGCATCCGCTATGAAGCGTTATCGTTAA

Upstream 100 bases:

>100_bases
TAGGATTTTTCTAGGATAGGGACTGGGGAAGTTCAGGAAAGAAAATTGTTTTTTTTGCTATACTTTCCTCCAACTGTCTA
GGTTGGGTAATGGTAAAATT

Downstream 100 bases:

>100_bases
TAAAGGCTTGGATATTATTTTCTTCTGCCCAAGCTAAAGCTTCTTTTTTTGTTGCAAATGTCATTAGTACTGTCGCCATG
GCATCAGCATACGCACAACT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MASKEIVSNRKALHNYEVLESLEAGIVLTGTEIKSLRDHGGNLGDAYVAISKGEAWLLNASIAPYRFGNIYNHEERRKRK
LLLHRYEIHKLEVKVAQKGVTIIPLGMFLSRGYVKVRLGCCRGKKSHDKRQTIIAREKEREIASAMKRYR

Sequences:

>Translated_150_residues
MASKEIVSNRKALHNYEVLESLEAGIVLTGTEIKSLRDHGGNLGDAYVAISKGEAWLLNASIAPYRFGNIYNHEERRKRK
LLLHRYEIHKLEVKVAQKGVTIIPLGMFLSRGYVKVRLGCCRGKKSHDKRQTIIAREKEREIASAMKRYR
>Mature_149_residues
ASKEIVSNRKALHNYEVLESLEAGIVLTGTEIKSLRDHGGNLGDAYVAISKGEAWLLNASIAPYRFGNIYNHEERRKRKL
LLHRYEIHKLEVKVAQKGVTIIPLGMFLSRGYVKVRLGCCRGKKSHDKRQTIIAREKEREIASAMKRYR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=38.3561643835616, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CHLAB (Q5L646)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219846.1
- HSSP:   Q8RR57
- ProteinModelPortal:   Q5L646
- SMR:   Q5L646
- GeneID:   3338207
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB432
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   CABO218497:CAB432-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17161; Mature: 17030

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKEIVSNRKALHNYEVLESLEAGIVLTGTEIKSLRDHGGNLGDAYVAISKGEAWLLNA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCCEEEEEECCCEEEEEC
SIAPYRFGNIYNHEERRKRKLLLHRYEIHKLEVKVAQKGVTIIPLGMFLSRGYVKVRLGC
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCEEEEHHHHHHCCEEEEEEEE
CRGKKSHDKRQTIIAREKEREIASAMKRYR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASKEIVSNRKALHNYEVLESLEAGIVLTGTEIKSLRDHGGNLGDAYVAISKGEAWLLNA
CCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCCEEEEEECCCEEEEEC
SIAPYRFGNIYNHEERRKRKLLLHRYEIHKLEVKVAQKGVTIIPLGMFLSRGYVKVRLGC
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCEEEEHHHHHHCCEEEEEEEE
CRGKKSHDKRQTIIAREKEREIASAMKRYR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA