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The map label for this gene is nifS1 [H]

Identifier: 62185016

GI number: 62185016

Start: 444701

End: 445816

Strand: Direct

Name: nifS1 [H]

Synonym: CAB387

Alternate gene names: 62185016

Gene position: 444701-445816 (Clockwise)

Preceding gene: 62185015

Following gene: 62185021

Centisome position: 38.86

GC content: 41.22

Gene sequence:

>1116_bases
ATGATTTATTTGGATAATAACGCTATGGCTTCTTTGGAGCCGGGGCTTTTAGATTTTCTACAGCAACTGTTTAGTCAGGG
CATTTATGCGAATCCTTCAAGCGTCCATAGTGCCGGAAAGAAATCTCGAAAACTCATTCATGATACTACCGGTTTAATTC
AGAGAGTTCTGTCTTTTCCTGGAAAGATAATTTATACGTCAAGTGCCACAGAAAGTTTGAATCTTGCTATTGCTAGCCTT
CCTAAAGGCAGTCATGTCATTACCTGTAGTAGTGAACATCCGGCGATTATCGAGCCTTTAAAACATGCTGATCTTTCCGT
TTCCTATTTAGATCCCGAACAAGGAAGTTGTGTAGTTAGTCCTCAGCAAATAGAAGCCGCGATTCGCCCAACCACTTCCG
CTATTGTTTTGGGTTGGGTAAATAGCGAAATTGGCGCAAAAATCCATATAGAGGCAATAGCACAGATCGCTAACGAACGT
CATCTACAATTTATTGTAGATGCTACAGCTATTATAGGTAGAGAGAGGGTGAGTATTCCTCAAGGCGTGACTATGGTTGC
CTTCAGTGGACATAAGTTTCATGCACTCTCAGGAATAGGTGCTCTTCTTATCTCTTCAGGGGTGAAGGTCGCTCCTTTAA
TTTGGGGCGGTGGTCAGCAAGGAGGAGTACGTTCAGGAACAGAAAATCTCTGGGGTATAGCTTCGCTGTTTTATATTTTC
ACCATGTTAGAGAAACAGCAGGAAGAAATAGCCGAAAGAATTGCACATTATCGCAATAACTTTGAAAAACGTCTGAAAGA
ACATATTCCTGAGATGCATATTCATTGTGAGCATCAACCTCGAGTGAATAATATTTCCGCTATAGCTTTCCCTCCTTTAG
AAGGTGAGGTCATGCAAATCGCTTTAGATATCGAAGGTATCGCCTGTGGCTATGGTTCTGCATGTTCTTCTGGAGCTACT
ACAGCTTTTAAATCCTTAGTTGCTATGAACATGGAACAGGATGTTGCTACAGCCACGTTAAGGTTTTCTTTCTCTCATTT
ATTAGCTCAGGAAGATCTGGATTTGGCTATAGAAAAGATTGTCAAGGTAGTTACTCGATTAAAAGATGCTTGGTAA

Upstream 100 bases:

>100_bases
GGAATATTTTAATTTCTCTAGCCAATAGTCGTGGGGGATCCGATAACGTTACCATAGTGTTAGTCCGAATACAATAGTAT
CCGTTTTAAGGAACTTTGTA

Downstream 100 bases:

>100_bases
GACCTACGAGAGCAGATTCTTAATTGAGACACTTTTAATGCCATATAATGAAACTTCTTTAACTTCTAAAAGAAGGTTAT
CCGTAATTACGGATGTACCA

Product: putative cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIYLDNNAMASLEPGLLDFLQQLFSQGIYANPSSVHSAGKKSRKLIHDTTGLIQRVLSFPGKIIYTSSATESLNLAIASL
PKGSHVITCSSEHPAIIEPLKHADLSVSYLDPEQGSCVVSPQQIEAAIRPTTSAIVLGWVNSEIGAKIHIEAIAQIANER
HLQFIVDATAIIGRERVSIPQGVTMVAFSGHKFHALSGIGALLISSGVKVAPLIWGGGQQGGVRSGTENLWGIASLFYIF
TMLEKQQEEIAERIAHYRNNFEKRLKEHIPEMHIHCEHQPRVNNISAIAFPPLEGEVMQIALDIEGIACGYGSACSSGAT
TAFKSLVAMNMEQDVATATLRFSFSHLLAQEDLDLAIEKIVKVVTRLKDAW

Sequences:

>Translated_371_residues
MIYLDNNAMASLEPGLLDFLQQLFSQGIYANPSSVHSAGKKSRKLIHDTTGLIQRVLSFPGKIIYTSSATESLNLAIASL
PKGSHVITCSSEHPAIIEPLKHADLSVSYLDPEQGSCVVSPQQIEAAIRPTTSAIVLGWVNSEIGAKIHIEAIAQIANER
HLQFIVDATAIIGRERVSIPQGVTMVAFSGHKFHALSGIGALLISSGVKVAPLIWGGGQQGGVRSGTENLWGIASLFYIF
TMLEKQQEEIAERIAHYRNNFEKRLKEHIPEMHIHCEHQPRVNNISAIAFPPLEGEVMQIALDIEGIACGYGSACSSGAT
TAFKSLVAMNMEQDVATATLRFSFSHLLAQEDLDLAIEKIVKVVTRLKDAW
>Mature_371_residues
MIYLDNNAMASLEPGLLDFLQQLFSQGIYANPSSVHSAGKKSRKLIHDTTGLIQRVLSFPGKIIYTSSATESLNLAIASL
PKGSHVITCSSEHPAIIEPLKHADLSVSYLDPEQGSCVVSPQQIEAAIRPTTSAIVLGWVNSEIGAKIHIEAIAQIANER
HLQFIVDATAIIGRERVSIPQGVTMVAFSGHKFHALSGIGALLISSGVKVAPLIWGGGQQGGVRSGTENLWGIASLFYIF
TMLEKQQEEIAERIAHYRNNFEKRLKEHIPEMHIHCEHQPRVNNISAIAFPPLEGEVMQIALDIEGIACGYGSACSSGAT
TAFKSLVAMNMEQDVATATLRFSFSHLLAQEDLDLAIEKIVKVVTRLKDAW

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=29.6103896103896, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=28.0575539568345, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI48994898, Length=382, Percent_Identity=30.3664921465969, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25143064, Length=383, Percent_Identity=25.8485639686684, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=25.8566978193146, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=27.7634961439589, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI20129463, Length=381, Percent_Identity=26.7716535433071, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40297; Mature: 40297

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNNAMASLEPGLLDFLQQLFSQGIYANPSSVHSAGKKSRKLIHDTTGLIQRVLSFP
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKIIYTSSATESLNLAIASLPKGSHVITCSSEHPAIIEPLKHADLSVSYLDPEQGSCVVS
CCEEEECCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCEEEC
PQQIEAAIRPTTSAIVLGWVNSEIGAKIHIEAIAQIANERHLQFIVDATAIIGRERVSIP
HHHHHHHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEEHHHHHCCHHCCCC
QGVTMVAFSGHKFHALSGIGALLISSGVKVAPLIWGGGQQGGVRSGTENLWGIASLFYIF
CCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
TMLEKQQEEIAERIAHYRNNFEKRLKEHIPEMHIHCEHQPRVNNISAIAFPPLEGEVMQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEE
ALDIEGIACGYGSACSSGATTAFKSLVAMNMEQDVATATLRFSFSHLLAQEDLDLAIEKI
EEEECEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVVTRLKDAW
HHHHHHHHCCC
>Mature Secondary Structure
MIYLDNNAMASLEPGLLDFLQQLFSQGIYANPSSVHSAGKKSRKLIHDTTGLIQRVLSFP
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKIIYTSSATESLNLAIASLPKGSHVITCSSEHPAIIEPLKHADLSVSYLDPEQGSCVVS
CCEEEECCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCEEEC
PQQIEAAIRPTTSAIVLGWVNSEIGAKIHIEAIAQIANERHLQFIVDATAIIGRERVSIP
HHHHHHHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEEHHHHHCCHHCCCC
QGVTMVAFSGHKFHALSGIGALLISSGVKVAPLIWGGGQQGGVRSGTENLWGIASLFYIF
CCCEEEEECCCCEEHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
TMLEKQQEEIAERIAHYRNNFEKRLKEHIPEMHIHCEHQPRVNNISAIAFPPLEGEVMQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEE
ALDIEGIACGYGSACSSGATTAFKSLVAMNMEQDVATATLRFSFSHLLAQEDLDLAIEKI
EEEECEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVVTRLKDAW
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]