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The map label for this gene is ruvB

Identifier: 62185021

GI number: 62185021

Start: 449788

End: 450801

Strand: Direct

Name: ruvB

Synonym: CAB392

Alternate gene names: 62185021

Gene position: 449788-450801 (Clockwise)

Preceding gene: 62185016

Following gene: 62185022

Centisome position: 39.3

GC content: 41.62

Gene sequence:

>1014_bases
ATGACACATCAAGTATCTGTCTTACATCAAGATAAAAAGTTCGATGTTTCTTTAAGGCCTAAGGGTTTAAAAGAGTTTTG
CGGTCAAGCTCAGCTTACAGAACGATTGGAGTTGTTTCTTAATGCTGCGATACAACGAGGGGAAGTCCCTGGGCATTGTC
TTTTTTTCGGTCCTCCGGGGTTGGGGAAAACCTCACTCGCGCATATTGTTGCAAACACAGTAGGAAAAGGGCTGCTTGTT
GCTTCAGGCCCGCAATTAGTTAAACCCTCTGATTTATTAGGGTTATTAACGAGTTTACAAGAAGGGGACGTTTTCTTTAT
TGATGAAATCCACCGTATGGGAAAGGTGGCTGAAGAGTATTTATACTCTGCAATGGAAGACTATAAGATTGACATCACCA
TTGATTCAGGTCCCGGTGCACGGTCTGTCTCTGTAGATCTCGCTCCTTTTAGTTTGGTTGGAGCTACAACCCGATCCGGC
ATGTTGAGCGAACCTCTCCGCGCACGTTTTTCTTTTACTGGCAGAGTAGCGTATTATTCCGACGAAGATTTAGCGACAAT
TCTTCGACGATCGTCAAATTTATTAGGGATTGATGCAGATGCTTCTGCTCTATACGAAATTGCGCGAAGATCTAGAGGAA
CACCTAGGTTAGCCAATAACCTTTTGCGTTGGGTACGCGATTTTGCACAGATGCGCGAGGGGAATTGCATCAATAGTGAC
GTAGCCGAAAAAGCTTTAGCTATGCTATTAATAGATGAGTGGGGATTAAATGAGATTGATATTAAACTCCTCACTACAAT
AATGAATTATTATCAGGGTGGTCCTGTAGGAATTAAAACTTTGTCAGTGGCCGTAGGAGAAGACGTCAGAACTTTAGAAG
ATGTATATGAGCCTTTTTTAATTTTAAAAGGTTTATTAAAGAAAACATCCCGAGGGAGAATGGTTACCCAACTTGCCTAT
AATCATTTGAAAAGGTGTTCGGATAATTTGCAGAGTTTAGGAGAAGAAAAGTGA

Upstream 100 bases:

>100_bases
CGATCTGGCTTCAAAGACATCATCCTCATAGGATGGGGGCAAATTTCTTAGAAGACAGAAATTCCTATCCTTTGTGAGAC
TAAAGGAAATGCGGTAATAT

Downstream 100 bases:

>100_bases
AGATATTGAAGTACATCCTTTTGGGACTTTCCTTCAGTATGGGTGTAGCCGGGTACACAGAAGTTAAGGTTTCTGATACG
TTTACGGTGCAACCTATTGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTHQVSVLHQDKKFDVSLRPKGLKEFCGQAQLTERLELFLNAAIQRGEVPGHCLFFGPPGLGKTSLAHIVANTVGKGLLV
ASGPQLVKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDYKIDITIDSGPGARSVSVDLAPFSLVGATTRSG
MLSEPLRARFSFTGRVAYYSDEDLATILRRSSNLLGIDADASALYEIARRSRGTPRLANNLLRWVRDFAQMREGNCINSD
VAEKALAMLLIDEWGLNEIDIKLLTTIMNYYQGGPVGIKTLSVAVGEDVRTLEDVYEPFLILKGLLKKTSRGRMVTQLAY
NHLKRCSDNLQSLGEEK

Sequences:

>Translated_337_residues
MTHQVSVLHQDKKFDVSLRPKGLKEFCGQAQLTERLELFLNAAIQRGEVPGHCLFFGPPGLGKTSLAHIVANTVGKGLLV
ASGPQLVKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDYKIDITIDSGPGARSVSVDLAPFSLVGATTRSG
MLSEPLRARFSFTGRVAYYSDEDLATILRRSSNLLGIDADASALYEIARRSRGTPRLANNLLRWVRDFAQMREGNCINSD
VAEKALAMLLIDEWGLNEIDIKLLTTIMNYYQGGPVGIKTLSVAVGEDVRTLEDVYEPFLILKGLLKKTSRGRMVTQLAY
NHLKRCSDNLQSLGEEK
>Mature_336_residues
THQVSVLHQDKKFDVSLRPKGLKEFCGQAQLTERLELFLNAAIQRGEVPGHCLFFGPPGLGKTSLAHIVANTVGKGLLVA
SGPQLVKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDYKIDITIDSGPGARSVSVDLAPFSLVGATTRSGM
LSEPLRARFSFTGRVAYYSDEDLATILRRSSNLLGIDADASALYEIARRSRGTPRLANNLLRWVRDFAQMREGNCINSDV
AEKALAMLLIDEWGLNEIDIKLLTTIMNYYQGGPVGIKTLSVAVGEDVRTLEDVYEPFLILKGLLKKTSRGRMVTQLAYN
HLKRCSDNLQSLGEEK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=309, Percent_Identity=50.4854368932039, Blast_Score=327, Evalue=8e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_CHLAB (Q5L686)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219806.1
- ProteinModelPortal:   Q5L686
- SMR:   Q5L686
- GeneID:   3338196
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB392
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   CABO218497:CAB392-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37195; Mature: 37064

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHQVSVLHQDKKFDVSLRPKGLKEFCGQAQLTERLELFLNAAIQRGEVPGHCLFFGPPG
CCCCCCEEECCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LGKTSLAHIVANTVGKGLLVASGPQLVKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEY
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
LYSAMEDYKIDITIDSGPGARSVSVDLAPFSLVGATTRSGMLSEPLRARFSFTGRVAYYS
HHHHHHCCEEEEEEECCCCCCEEEEECCCHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEC
DEDLATILRRSSNLLGIDADASALYEIARRSRGTPRLANNLLRWVRDFAQMREGNCINSD
CHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
VAEKALAMLLIDEWGLNEIDIKLLTTIMNYYQGGPVGIKTLSVAVGEDVRTLEDVYEPFL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHH
ILKGLLKKTSRGRMVTQLAYNHLKRCSDNLQSLGEEK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
THQVSVLHQDKKFDVSLRPKGLKEFCGQAQLTERLELFLNAAIQRGEVPGHCLFFGPPG
CCCCCEEECCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LGKTSLAHIVANTVGKGLLVASGPQLVKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEY
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
LYSAMEDYKIDITIDSGPGARSVSVDLAPFSLVGATTRSGMLSEPLRARFSFTGRVAYYS
HHHHHHCCEEEEEEECCCCCCEEEEECCCHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEC
DEDLATILRRSSNLLGIDADASALYEIARRSRGTPRLANNLLRWVRDFAQMREGNCINSD
CHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
VAEKALAMLLIDEWGLNEIDIKLLTTIMNYYQGGPVGIKTLSVAVGEDVRTLEDVYEPFL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHH
ILKGLLKKTSRGRMVTQLAYNHLKRCSDNLQSLGEEK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA