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The map label for this gene is ptsI [H]

Identifier: 62184955

GI number: 62184955

Start: 377242

End: 378942

Strand: Direct

Name: ptsI [H]

Synonym: CAB325

Alternate gene names: 62184955

Gene position: 377242-378942 (Clockwise)

Preceding gene: 62184954

Following gene: 62184958

Centisome position: 32.96

GC content: 40.56

Gene sequence:

>1701_bases
ATGAACACACCCACGCCTTCTCTAGAGCAAAATAAGGAATGGCGAGTTCCTGGAATGACTTTAGTTCCTGGGGTAGCTAT
AGGAAAAGCATTTTTCTTAGGTACTTCTCCTCTGCAAATTCATGAACTTACCCTACCTCAAGAAGAGGTGGAACACGAAA
TACACCGGTATTATAAAGCATTAAACCGTTCTAAATCTGATATCGTTGCCTTAGAACAAGAAGCTCAGGGTAAGCAAGGT
CAACAAGAAATCTCTTCAATACTCCAAGCACATCTGGAGATCATTAAAGATCCTATTTTGACAGAAGAGGTTGTAAATAC
CATCAGAAAAGATCGTAAAAACGCCGAATATGTCTTTTCTTCTGTTATGGAAAAAATCGAGGAGTCATTAACAGCAGTTC
AAGGGACTTCTCTAGCTGTAGATCGTGTTCAGGATATCCATGATATTTCTAATCGTGTGATTGGTCATTTGTGCTGTCAG
CATAAGAGTTCTTTAGGTGATGCAGATCAAAATATCATCGTTTTCTCTAAAGAACTCACGCCTTCAGAAGTGGCTAGCGC
TAATCCCTCATATATCCGTGGATTTGTTTCTTTTATTGGGGCGCCGACATCGCATACAGCTATCGTTTCTAGAGCGAAAA
ATATTCCCTATTTAGCGAATTTTTCTCAGGAAAACTGGGAGCGTATTCAAGGGTATACCGGGAAACTGGTCTTGATAGAC
GGTATTCGTGGTGAGATTATTTTTAATCCTAAATCCAAAACTCTTGAAAATTGTTATAAGCAAAAGAGTACCGCATATAG
TGTAAAATCGTATCCTCAACCGTCTCCACACGCGATAGTCTCTTCTCATGCTGCTAGCCTCGAAGAGCTTCGCATGCTTT
CGGAGTTCTTTCCACAAACTTCTATCGGGTTATTTCGTTCTGAATTTCTCGCTATAGCTGAAGATCGTTTACCTACAGTA
GAAGAACAAGCGGCTGTTTATAAATCTTTAGCCTTGTTTCCTGAGCGGGTTTCTGTATTACGTTTATTTGATTTTGGCGA
AGATAAGCTCTGTCCCGGTCAAAAGCCCATCAAAGAGCGCTCCATACGTTATTTATTGAAAAACTCGCCTGTGCTTGACG
ATCAGCTTTGTGCTATTTTAACAGCTTCAGTATCAGGTTCTTTGAAAGTGTTAATTCCTGGAACTGCTGATGTTATAGAA
ATTATCGAAGTGAAACGTCGGTTAGAGAATATACGCCGTTCTTTTAACAAAGATCATGCCATAGAAAATATTGCCTGGGG
CAGCATGATAGAACTTCCTTCGGCCGTATTGATGATTGATGAGATCCTTCAGGAATGTGATTTTATTTCCATAGGAACGA
ATGATCTCATGCAATATACCCTAGGGAATAATCGAGAAACTCTACTTCCTCATTATCTTGATAATCCACTACACCCTTCG
GTCATGCGCATGATTCGTCATGTAGTGTCTAGTGCGAAACAGCGCGATGTTCACGTATCTATTTGTGGGGAGGCTGCAGC
AAATCTTTCCTTAACACCATTTTTCTTAGGCTTGGGAGTCCAAGAACTCTCAGTGGCTATGCCAGCAATTGTAGAACTGC
GAGAAAGAATAGCATCTCTAAATTTCAGTGATTGTGTAGAACATACTGAAAAGTTATTAAGAGCCAGGACCTGTGCAGAA
GTTCAGGCCCTGTTAGCTTAA

Upstream 100 bases:

>100_bases
ACAAAACGGAGAGATTTTAGTGCGTATTAAAGGGAAAGATGCAAGTCGGGTATTGCAAAAAGTGCAGAATGCCTTTGACT
CAGGTTTTGGAGAGTTGTAA

Downstream 100 bases:

>100_bases
AAAGGCATGCTGGCACGCATTACAGACAATTCTTTGTCCATCGCCTCTTTAGCCTCTTTAAATGCTGAATGGAAAAGATC
TTCAATAACTTCAGGATCTT

Product: putative phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MNTPTPSLEQNKEWRVPGMTLVPGVAIGKAFFLGTSPLQIHELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEAQGKQG
QQEISSILQAHLEIIKDPILTEEVVNTIRKDRKNAEYVFSSVMEKIEESLTAVQGTSLAVDRVQDIHDISNRVIGHLCCQ
HKSSLGDADQNIIVFSKELTPSEVASANPSYIRGFVSFIGAPTSHTAIVSRAKNIPYLANFSQENWERIQGYTGKLVLID
GIRGEIIFNPKSKTLENCYKQKSTAYSVKSYPQPSPHAIVSSHAASLEELRMLSEFFPQTSIGLFRSEFLAIAEDRLPTV
EEQAAVYKSLALFPERVSVLRLFDFGEDKLCPGQKPIKERSIRYLLKNSPVLDDQLCAILTASVSGSLKVLIPGTADVIE
IIEVKRRLENIRRSFNKDHAIENIAWGSMIELPSAVLMIDEILQECDFISIGTNDLMQYTLGNNRETLLPHYLDNPLHPS
VMRMIRHVVSSAKQRDVHVSICGEAAANLSLTPFFLGLGVQELSVAMPAIVELRERIASLNFSDCVEHTEKLLRARTCAE
VQALLA

Sequences:

>Translated_566_residues
MNTPTPSLEQNKEWRVPGMTLVPGVAIGKAFFLGTSPLQIHELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEAQGKQG
QQEISSILQAHLEIIKDPILTEEVVNTIRKDRKNAEYVFSSVMEKIEESLTAVQGTSLAVDRVQDIHDISNRVIGHLCCQ
HKSSLGDADQNIIVFSKELTPSEVASANPSYIRGFVSFIGAPTSHTAIVSRAKNIPYLANFSQENWERIQGYTGKLVLID
GIRGEIIFNPKSKTLENCYKQKSTAYSVKSYPQPSPHAIVSSHAASLEELRMLSEFFPQTSIGLFRSEFLAIAEDRLPTV
EEQAAVYKSLALFPERVSVLRLFDFGEDKLCPGQKPIKERSIRYLLKNSPVLDDQLCAILTASVSGSLKVLIPGTADVIE
IIEVKRRLENIRRSFNKDHAIENIAWGSMIELPSAVLMIDEILQECDFISIGTNDLMQYTLGNNRETLLPHYLDNPLHPS
VMRMIRHVVSSAKQRDVHVSICGEAAANLSLTPFFLGLGVQELSVAMPAIVELRERIASLNFSDCVEHTEKLLRARTCAE
VQALLA
>Mature_566_residues
MNTPTPSLEQNKEWRVPGMTLVPGVAIGKAFFLGTSPLQIHELTLPQEEVEHEIHRYYKALNRSKSDIVALEQEAQGKQG
QQEISSILQAHLEIIKDPILTEEVVNTIRKDRKNAEYVFSSVMEKIEESLTAVQGTSLAVDRVQDIHDISNRVIGHLCCQ
HKSSLGDADQNIIVFSKELTPSEVASANPSYIRGFVSFIGAPTSHTAIVSRAKNIPYLANFSQENWERIQGYTGKLVLID
GIRGEIIFNPKSKTLENCYKQKSTAYSVKSYPQPSPHAIVSSHAASLEELRMLSEFFPQTSIGLFRSEFLAIAEDRLPTV
EEQAAVYKSLALFPERVSVLRLFDFGEDKLCPGQKPIKERSIRYLLKNSPVLDDQLCAILTASVSGSLKVLIPGTADVIE
IIEVKRRLENIRRSFNKDHAIENIAWGSMIELPSAVLMIDEILQECDFISIGTNDLMQYTLGNNRETLLPHYLDNPLHPS
VMRMIRHVVSSAKQRDVHVSICGEAAANLSLTPFFLGLGVQELSVAMPAIVELRERIASLNFSDCVEHTEKLLRARTCAE
VQALLA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=569, Percent_Identity=26.8892794376098, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI48994992, Length=508, Percent_Identity=27.3622047244095, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789193, Length=603, Percent_Identity=25.3731343283582, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788726, Length=581, Percent_Identity=27.0223752151463, Blast_Score=162, Evalue=8e-41,
Organism=Escherichia coli, GI226510935, Length=209, Percent_Identity=24.8803827751196, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63153; Mature: 63153

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPTPSLEQNKEWRVPGMTLVPGVAIGKAFFLGTSPLQIHELTLPQEEVEHEIHRYYKA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHHH
LNRSKSDIVALEQEAQGKQGQQEISSILQAHLEIIKDPILTEEVVNTIRKDRKNAEYVFS
HCCCCCHHEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
SVMEKIEESLTAVQGTSLAVDRVQDIHDISNRVIGHLCCQHKSSLGDADQNIIVFSKELT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PSEVASANPSYIRGFVSFIGAPTSHTAIVSRAKNIPYLANFSQENWERIQGYTGKLVLID
HHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEEEE
GIRGEIIFNPKSKTLENCYKQKSTAYSVKSYPQPSPHAIVSSHAASLEELRMLSEFFPQT
CCCCEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
SIGLFRSEFLAIAEDRLPTVEEQAAVYKSLALFPERVSVLRLFDFGEDKLCPGQKPIKER
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
SIRYLLKNSPVLDDQLCAILTASVSGSLKVLIPGTADVIEIIEVKRRLENIRRSFNKDHA
HHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHH
IENIAWGSMIELPSAVLMIDEILQECDFISIGTNDLMQYTLGNNRETLLPHYLDNPLHPS
HHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCEECHHHCCCCCCHH
VMRMIRHVVSSAKQRDVHVSICGEAAANLSLTPFFLGLGVQELSVAMPAIVELRERIASL
HHHHHHHHHHHHHHCCEEEEEECHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
NFSDCVEHTEKLLRARTCAEVQALLA
CHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNTPTPSLEQNKEWRVPGMTLVPGVAIGKAFFLGTSPLQIHELTLPQEEVEHEIHRYYKA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHHH
LNRSKSDIVALEQEAQGKQGQQEISSILQAHLEIIKDPILTEEVVNTIRKDRKNAEYVFS
HCCCCCHHEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
SVMEKIEESLTAVQGTSLAVDRVQDIHDISNRVIGHLCCQHKSSLGDADQNIIVFSKELT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PSEVASANPSYIRGFVSFIGAPTSHTAIVSRAKNIPYLANFSQENWERIQGYTGKLVLID
HHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEEEE
GIRGEIIFNPKSKTLENCYKQKSTAYSVKSYPQPSPHAIVSSHAASLEELRMLSEFFPQT
CCCCEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
SIGLFRSEFLAIAEDRLPTVEEQAAVYKSLALFPERVSVLRLFDFGEDKLCPGQKPIKER
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
SIRYLLKNSPVLDDQLCAILTASVSGSLKVLIPGTADVIEIIEVKRRLENIRRSFNKDHA
HHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHH
IENIAWGSMIELPSAVLMIDEILQECDFISIGTNDLMQYTLGNNRETLLPHYLDNPLHPS
HHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCEECHHHCCCCCCHH
VMRMIRHVVSSAKQRDVHVSICGEAAANLSLTPFFLGLGVQELSVAMPAIVELRERIASL
HHHHHHHHHHHHHHCCEEEEEECHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
NFSDCVEHTEKLLRARTCAEVQALLA
CHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]