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The map label for this gene is artJ [H]

Identifier: 62184897

GI number: 62184897

Start: 280899

End: 281663

Strand: Direct

Name: artJ [H]

Synonym: CAB256

Alternate gene names: 62184897

Gene position: 280899-281663 (Clockwise)

Preceding gene: 62184894

Following gene: 62184898

Centisome position: 24.55

GC content: 42.48

Gene sequence:

>765_bases
ATGAAACAGTGGATTTCTTGCCTTAGAATCTTGTTTTTCGGGTGTTGTTTACTCTTAGTTGGTTGCAATTCTTCTGTTAA
GAGAGATAAAATTTGGCTAGTAGGTACGAATGCGACTTACCCCCCCTTTGAATTTATTGATAAACATGGAAACACCATTG
GTTTTGATATAGATCTTGCCGAAGCCATTAGCCAAAGACTTGGTAAAGAATTGAAGGTGGTGGAGTTCTCTTTTGACGCG
TTGATTCTCAATTTAAAGAAACATCGCATAGATGCGGTTCTAGCAGGGATGTCCATTACACCCTCGCGTCAAAAAGAAAT
TGCTATGCTTCCCTATCATGGAGAGGGCATCCATGAGCTCACGATTGTATCTAGACAGCCTATAAGTAAAGATGATGTTC
TTCCTTTGTCTAAGTATTCTTCAGTAGCTGTACAAACGGGGACTTTCCAAGAGGATTACCTGCTGTCTTTACCCGGAGTC
TGTGTCCGTTCTTTTGATAGCACTTTAGAGGTTCTAATGGAGGTGGGGTGTAAGAAGTCCCCAATCGCTGTGCTTGAGCC
TTCTGTGGCGCATGTGGTACTGAAAGAGTTTCCAGATGTATATACAACAACCGTAGACTTACCTGAAGATCAGTGGGTTT
TAGGGTGCGGCTTAGGTATAGCAAAAGATCGCCCTGAGCACGTGAAAGAAGTCCAAAACGTGTTGGAGGAACTGCGAGCA
GACGGAACCCTCCTAAAATTAGAAAAAAAATGGGGATTACATTAA

Upstream 100 bases:

>100_bases
CTCGCTTTCGATGTCAGTTAATTTAAAAGCATATTTCGAGTTTGAGATTTATTGTGTATGTAAAGTATACCATAATTTTA
GTTATTATAGCATAGTTTTT

Downstream 100 bases:

>100_bases
CAAGACGTTTCTCATCTTTGTATTAAGCAGGCCCTTTTATCTATTTTTTCGATCGGTATTTTTTTAAACAGGTGAGATTA
CTTTCTGTTATATAAGAATA

Product: membrane transport protein, solute binding component

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKQWISCLRILFFGCCLLLVGCNSSVKRDKIWLVGTNATYPPFEFIDKHGNTIGFDIDLAEAISQRLGKELKVVEFSFDA
LILNLKKHRIDAVLAGMSITPSRQKEIAMLPYHGEGIHELTIVSRQPISKDDVLPLSKYSSVAVQTGTFQEDYLLSLPGV
CVRSFDSTLEVLMEVGCKKSPIAVLEPSVAHVVLKEFPDVYTTTVDLPEDQWVLGCGLGIAKDRPEHVKEVQNVLEELRA
DGTLLKLEKKWGLH

Sequences:

>Translated_254_residues
MKQWISCLRILFFGCCLLLVGCNSSVKRDKIWLVGTNATYPPFEFIDKHGNTIGFDIDLAEAISQRLGKELKVVEFSFDA
LILNLKKHRIDAVLAGMSITPSRQKEIAMLPYHGEGIHELTIVSRQPISKDDVLPLSKYSSVAVQTGTFQEDYLLSLPGV
CVRSFDSTLEVLMEVGCKKSPIAVLEPSVAHVVLKEFPDVYTTTVDLPEDQWVLGCGLGIAKDRPEHVKEVQNVLEELRA
DGTLLKLEKKWGLH
>Mature_254_residues
MKQWISCLRILFFGCCLLLVGCNSSVKRDKIWLVGTNATYPPFEFIDKHGNTIGFDIDLAEAISQRLGKELKVVEFSFDA
LILNLKKHRIDAVLAGMSITPSRQKEIAMLPYHGEGIHELTIVSRQPISKDDVLPLSKYSSVAVQTGTFQEDYLLSLPGV
CVRSFDSTLEVLMEVGCKKSPIAVLEPSVAHVVLKEFPDVYTTTVDLPEDQWVLGCGLGIAKDRPEHVKEVQNVLEELRA
DGTLLKLEKKWGLH

Specific function: Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Secreted. Cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=30.6306306306306, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787088, Length=240, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=29.5154185022026, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788649, Length=227, Percent_Identity=30.8370044052863, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=27.6595744680851, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=27.9835390946502, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28350; Mature: 28350

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQWISCLRILFFGCCLLLVGCNSSVKRDKIWLVGTNATYPPFEFIDKHGNTIGFDIDLA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEECHHH
EAISQRLGKELKVVEFSFDALILNLKKHRIDAVLAGMSITPSRQKEIAMLPYHGEGIHEL
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEE
TIVSRQPISKDDVLPLSKYSSVAVQTGTFQEDYLLSLPGVCVRSFDSTLEVLMEVGCKKS
EEEECCCCCCCCCCCCHHCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCC
PIAVLEPSVAHVVLKEFPDVYTTTVDLPEDQWVLGCGLGIAKDRPEHVKEVQNVLEELRA
CCEEECCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHC
DGTLLKLEKKWGLH
CCCEEEEECCCCCC
>Mature Secondary Structure
MKQWISCLRILFFGCCLLLVGCNSSVKRDKIWLVGTNATYPPFEFIDKHGNTIGFDIDLA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEECHHH
EAISQRLGKELKVVEFSFDALILNLKKHRIDAVLAGMSITPSRQKEIAMLPYHGEGIHEL
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEE
TIVSRQPISKDDVLPLSKYSSVAVQTGTFQEDYLLSLPGVCVRSFDSTLEVLMEVGCKKS
EEEECCCCCCCCCCCCHHCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCC
PIAVLEPSVAHVVLKEFPDVYTTTVDLPEDQWVLGCGLGIAKDRPEHVKEVQNVLEELRA
CCEEECCHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHC
DGTLLKLEKKWGLH
CCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]