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The map label for this gene is dctA [H]

Identifier: 62184856

GI number: 62184856

Start: 233306

End: 234556

Strand: Direct

Name: dctA [H]

Synonym: CAB212

Alternate gene names: 62184856

Gene position: 233306-234556 (Clockwise)

Preceding gene: 62184855

Following gene: 62184857

Centisome position: 20.39

GC content: 41.89

Gene sequence:

>1251_bases
ATGAAACTATGGATGAAAATCTTTATAGGATTATTTGTTGGGGTTACCCTAGGTTTAATTTTAGAAGATAAAGCCATCTT
TTTTAAACCTATAGGAGATATTTTCTTAAACCTATTGAGCATGGTTGTCTACCCTCTGGTATTTTGTTCCATGGTGTTGG
GTATCGCTTCTATTAGTGATATGAAGAAGCTAGGACGCATAGGAGTGAAAAGCGTCGCCTTATATTTAGGGACAACATGT
CTAGCTATTGTTATAGGCTTATGTTTTGCTCAATTTTTCAGTCCTGGAGAGGGCTGTGACTTGTCTCAAAATGTCACGGA
AACGCAGATCGTGGCTCCTGAGAGAAGTAGTACGTATTTCTTGTCTTTGATTTCTCAAATTTTTCCTTCAAATCCTGTGA
GATCTTTCGTTGAGGGAAATATTTTACAAATCATAGTTTTTGCTATTTTCTTAGGAATCGCTATGCGTCTTTCTGGAGAA
CAGGGACGTCCAGTAGCTAAATTTATTGAGGGTTTTTCTGAAATCATGTTGCGCATGATCAATATGATCATGACCTTTGC
GCCTTACGGTGTCGGGGCGAGTATGGCATGGATTTCTGGAAGTCACGGTTTGGTTATTCTCTGGCAATTAGGGAAATTTG
TTTTCGCCTACTATCTTGCATGTCTATTTCATGCTGTGCTGGTTTTTGGTGGTATCATCCGCATGGGCTGTAGGATGTCC
TTCTCTAAATTCCTTTCTGCCATGATGGATGCCATATCTTGTGCAATATCCACTTCTAGTAGTTCGGCAACACTGCCTGT
GACTATGCGTTGCGTATCTAAAAATCTTGGAGTTTCTTCGGAAGTTTCTGGTTTTGTTTTGCCTTTAGGTGCTACTGTCA
ATATGAATGGTACGGCGATATTTCAAGGTATGGCGGCAGTATTTATTGCTCAGGCCTATAACTGTCCATTACCCTTCAGC
AGTTTGTTGTTAATCGTGATCGCAGCGACTTTCTCTGCGGTCGGTAGTGCGGGAGTCCCTGGAGGAGGCATGATTACTCT
GGGATCTGTATTAGCCTCTGTAGGGTTGCCTATTCAAGGCATTGCTGTTTTAGCAGGCATTGATAGGTTGCGAGACATCA
TAGGAACGCCTATGAACATCCTTGGAGATGCTGTAGTCGCTGTTTATGTGGCTTCCGGAGAAGGTGAGTTGTCCACACCT
TTGGAAGAGAAAAAGGTACTTTTGAAAGATGAGAGTACAGAGACAGTGTAG

Upstream 100 bases:

>100_bases
AGGTAAAGTGCAAATGGAATTTGATTTTACCCATCAAGAGGATGCTACAGCCGCCTTACTGGATAAAATAGATCGGATAC
ATAATGGTAAGAGGTAACTA

Downstream 100 bases:

>100_bases
GAGAGTTTGCACATGTTTGAATTTCGATTTCCGAAAATAGGCGAGTCTGGTTCTGGAGGGTTGGTAGTTCGTTGGCTCAA
GCAGGTGGGAGAGAACATTG

Product: putative symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKLWMKIFIGLFVGVTLGLILEDKAIFFKPIGDIFLNLLSMVVYPLVFCSMVLGIASISDMKKLGRIGVKSVALYLGTTC
LAIVIGLCFAQFFSPGEGCDLSQNVTETQIVAPERSSTYFLSLISQIFPSNPVRSFVEGNILQIIVFAIFLGIAMRLSGE
QGRPVAKFIEGFSEIMLRMINMIMTFAPYGVGASMAWISGSHGLVILWQLGKFVFAYYLACLFHAVLVFGGIIRMGCRMS
FSKFLSAMMDAISCAISTSSSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGTAIFQGMAAVFIAQAYNCPLPFS
SLLLIVIAATFSAVGSAGVPGGGMITLGSVLASVGLPIQGIAVLAGIDRLRDIIGTPMNILGDAVVAVYVASGEGELSTP
LEEKKVLLKDESTETV

Sequences:

>Translated_416_residues
MKLWMKIFIGLFVGVTLGLILEDKAIFFKPIGDIFLNLLSMVVYPLVFCSMVLGIASISDMKKLGRIGVKSVALYLGTTC
LAIVIGLCFAQFFSPGEGCDLSQNVTETQIVAPERSSTYFLSLISQIFPSNPVRSFVEGNILQIIVFAIFLGIAMRLSGE
QGRPVAKFIEGFSEIMLRMINMIMTFAPYGVGASMAWISGSHGLVILWQLGKFVFAYYLACLFHAVLVFGGIIRMGCRMS
FSKFLSAMMDAISCAISTSSSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGTAIFQGMAAVFIAQAYNCPLPFS
SLLLIVIAATFSAVGSAGVPGGGMITLGSVLASVGLPIQGIAVLAGIDRLRDIIGTPMNILGDAVVAVYVASGEGELSTP
LEEKKVLLKDESTETV
>Mature_416_residues
MKLWMKIFIGLFVGVTLGLILEDKAIFFKPIGDIFLNLLSMVVYPLVFCSMVLGIASISDMKKLGRIGVKSVALYLGTTC
LAIVIGLCFAQFFSPGEGCDLSQNVTETQIVAPERSSTYFLSLISQIFPSNPVRSFVEGNILQIIVFAIFLGIAMRLSGE
QGRPVAKFIEGFSEIMLRMINMIMTFAPYGVGASMAWISGSHGLVILWQLGKFVFAYYLACLFHAVLVFGGIIRMGCRMS
FSKFLSAMMDAISCAISTSSSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGTAIFQGMAAVFIAQAYNCPLPFS
SLLLIVIAATFSAVGSAGVPGGGMITLGSVLASVGLPIQGIAVLAGIDRLRDIIGTPMNILGDAVVAVYVASGEGELSTP
LEEKKVLLKDESTETV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=395, Percent_Identity=33.6708860759494, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI169790839, Length=433, Percent_Identity=31.1778290993072, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI5032093, Length=396, Percent_Identity=36.1111111111111, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI223468566, Length=296, Percent_Identity=38.5135135135135, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI40254478, Length=441, Percent_Identity=29.4784580498866, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI223468564, Length=271, Percent_Identity=38.7453874538745, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI66773030, Length=435, Percent_Identity=29.1954022988506, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI4827012, Length=479, Percent_Identity=27.9749478079332, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI194239697, Length=449, Percent_Identity=27.8396436525612, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI262359914, Length=373, Percent_Identity=29.7587131367292, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI301601644, Length=143, Percent_Identity=42.6573426573427, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1790514, Length=399, Percent_Identity=33.5839598997494, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789947, Length=408, Percent_Identity=32.843137254902, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788024, Length=380, Percent_Identity=25.7894736842105, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789473, Length=221, Percent_Identity=27.6018099547511, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17537407, Length=391, Percent_Identity=31.2020460358056, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=27.2935779816514, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=27.2935779816514, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71996953, Length=434, Percent_Identity=28.8018433179723, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI193206505, Length=437, Percent_Identity=29.0617848970252, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193206654, Length=420, Percent_Identity=28.0952380952381, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17541374, Length=392, Percent_Identity=29.5918367346939, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24583025, Length=429, Percent_Identity=26.8065268065268, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17137668, Length=429, Percent_Identity=26.8065268065268, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24583023, Length=429, Percent_Identity=26.8065268065268, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI281360483, Length=470, Percent_Identity=24.8936170212766, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137666, Length=470, Percent_Identity=24.8936170212766, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281360481, Length=452, Percent_Identity=25.2212389380531, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44189; Mature: 44189

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLWMKIFIGLFVGVTLGLILEDKAIFFKPIGDIFLNLLSMVVYPLVFCSMVLGIASISD
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKKLGRIGVKSVALYLGTTCLAIVIGLCFAQFFSPGEGCDLSQNVTETQIVAPERSSTYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCHHH
LSLISQIFPSNPVRSFVEGNILQIIVFAIFLGIAMRLSGEQGRPVAKFIEGFSEIMLRMI
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
NMIMTFAPYGVGASMAWISGSHGLVILWQLGKFVFAYYLACLFHAVLVFGGIIRMGCRMS
HHHHHHCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSKFLSAMMDAISCAISTSSSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGTAI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHH
FQGMAAVFIAQAYNCPLPFSSLLLIVIAATFSAVGSAGVPGGGMITLGSVLASVGLPIQG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHH
IAVLAGIDRLRDIIGTPMNILGDAVVAVYVASGEGELSTPLEEKKVLLKDESTETV
HHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLWMKIFIGLFVGVTLGLILEDKAIFFKPIGDIFLNLLSMVVYPLVFCSMVLGIASISD
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKKLGRIGVKSVALYLGTTCLAIVIGLCFAQFFSPGEGCDLSQNVTETQIVAPERSSTYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCHHH
LSLISQIFPSNPVRSFVEGNILQIIVFAIFLGIAMRLSGEQGRPVAKFIEGFSEIMLRMI
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
NMIMTFAPYGVGASMAWISGSHGLVILWQLGKFVFAYYLACLFHAVLVFGGIIRMGCRMS
HHHHHHCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSKFLSAMMDAISCAISTSSSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGTAI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHH
FQGMAAVFIAQAYNCPLPFSSLLLIVIAATFSAVGSAGVPGGGMITLGSVLASVGLPIQG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHH
IAVLAGIDRLRDIIGTPMNILGDAVVAVYVASGEGELSTPLEEKKVLLKDESTETV
HHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]