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The map label for this gene is bfmBB [H]

Identifier: 62184857

GI number: 62184857

Start: 234569

End: 235738

Strand: Direct

Name: bfmBB [H]

Synonym: CAB214

Alternate gene names: 62184857

Gene position: 234569-235738 (Clockwise)

Preceding gene: 62184856

Following gene: 62184858

Centisome position: 20.5

GC content: 41.97

Gene sequence:

>1170_bases
ATGTTTGAATTTCGATTTCCGAAAATAGGCGAGTCTGGTTCTGGAGGGTTGGTAGTTCGTTGGCTCAAGCAGGTGGGAGA
GAACATTGCAAAAGATGAGCCGGTAATTGAAGTATCTACTGACAAGATTGCTACAGAGTTAGCCTCCCCTAAAGCAGGGA
AATTAATGCGTTGTCTCGTCAAAGAAGGTGATGAAGTTGCTTCCGGAGAGATTATAGCCTTAATAGATACGGAATGTGCT
GTCGAGGAAGAGGTAGTTGTAGAAGAACCGTCACCTCATGCTTCTTGTCCTCAAGATTCTGGGAAGAATGCGGCATGGTT
TTCTCCGGCAGTTCTGAGTTTAGCACACCGTGAAGGTATTTCTATCCAGCAACTACAGCAGATTTCTGGAACAGGAAATG
ACGGTCGTGTCACGCGTAGGGATTTAGAAAACTACATTCTTGAAATGCGTCAACCTTCGTGCCCACATATTGCGAATGCG
AATGAAAATCGTATTCCTATGTCTCCGCTACGTAGAGCTATAGCTTCCTCGTTATCTAAATCTTCAGATGAAGTGCCTCA
TGCTTCTCTTATTGTGGATATTGATGTCACAGATTTGATGAATTTAATTGCTGAAGAGAAGGATAGATTTTTTGCCACGC
ATGGTGTGAAACTGACAATTACGAGTTTCATTATCCAGTGTTTAGCAAAAGCTTTGGAGCAATTCCCGCTATTGAATGGG
TCTTTAGACGGCGATACGATTGTTGTGAAGAAATCCATCAATGTGGGTGTGGCTGTGAATTTGAATAAAGAGGGCGTTGT
TGTTCCTGTTATTCATAATTGTCAGGATCGTGGTTTAGTAAGTATTGCGAAAACTCTTGCTGATCTTTCTGCAAGATCTC
GGGCAAATAAACTTGATCCTTCAGAAACGCAAGATGGTAGTGTTACGGTGACCAATTTTGGTATGACAGGAGCTTTAATT
GGCATGCCGATCATTCGTTATCCTGAAGTCGCTATTTTAGGCATAGGAACGATACAAAAACGTGTGGTTGTGCGTGATGA
CGATTCTTTAGCTATTCGCAAAATGGTGTATGTTACCCTAACATTTGACCACCGGGTACTTGATGGTATTTACGGTAGTG
AATTTTTAACCTCATTGAAAAATCGGTTAGAGTCTGTTACGATGAGCTAA

Upstream 100 bases:

>100_bases
TTATGTGGCTTCCGGAGAAGGTGAGTTGTCCACACCTTTGGAAGAGAAAAAGGTACTTTTGAAAGATGAGAGTACAGAGA
CAGTGTAGGAGAGTTTGCAC

Downstream 100 bases:

>100_bases
CACACAACGATTTTTCTGAGGACTCAAGGGATGCGCTCTCCCACAACATCTATTGATCTATGCCAAGATATTGTCAGTAA
GCAAAGGGAATCTTTAGAAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MFEFRFPKIGESGSGGLVVRWLKQVGENIAKDEPVIEVSTDKIATELASPKAGKLMRCLVKEGDEVASGEIIALIDTECA
VEEEVVVEEPSPHASCPQDSGKNAAWFSPAVLSLAHREGISIQQLQQISGTGNDGRVTRRDLENYILEMRQPSCPHIANA
NENRIPMSPLRRAIASSLSKSSDEVPHASLIVDIDVTDLMNLIAEEKDRFFATHGVKLTITSFIIQCLAKALEQFPLLNG
SLDGDTIVVKKSINVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKTLADLSARSRANKLDPSETQDGSVTVTNFGMTGALI
GMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMS

Sequences:

>Translated_389_residues
MFEFRFPKIGESGSGGLVVRWLKQVGENIAKDEPVIEVSTDKIATELASPKAGKLMRCLVKEGDEVASGEIIALIDTECA
VEEEVVVEEPSPHASCPQDSGKNAAWFSPAVLSLAHREGISIQQLQQISGTGNDGRVTRRDLENYILEMRQPSCPHIANA
NENRIPMSPLRRAIASSLSKSSDEVPHASLIVDIDVTDLMNLIAEEKDRFFATHGVKLTITSFIIQCLAKALEQFPLLNG
SLDGDTIVVKKSINVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKTLADLSARSRANKLDPSETQDGSVTVTNFGMTGALI
GMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMS
>Mature_389_residues
MFEFRFPKIGESGSGGLVVRWLKQVGENIAKDEPVIEVSTDKIATELASPKAGKLMRCLVKEGDEVASGEIIALIDTECA
VEEEVVVEEPSPHASCPQDSGKNAAWFSPAVLSLAHREGISIQQLQQISGTGNDGRVTRRDLENYILEMRQPSCPHIANA
NENRIPMSPLRRAIASSLSKSSDEVPHASLIVDIDVTDLMNLIAEEKDRFFATHGVKLTITSFIIQCLAKALEQFPLLNG
SLDGDTIVVKKSINVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKTLADLSARSRANKLDPSETQDGSVTVTNFGMTGALI
GMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=27.8801843317972, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI110671329, Length=414, Percent_Identity=27.2946859903382, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=32.7433628318584, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI203098816, Length=442, Percent_Identity=26.6968325791855, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI203098753, Length=239, Percent_Identity=31.7991631799163, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1786946, Length=399, Percent_Identity=28.8220551378446, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=28.2710280373832, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=396, Percent_Identity=28.7878787878788, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=416, Percent_Identity=28.3653846153846, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=28.0575539568345, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=31.3636363636364, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=386, Percent_Identity=27.2020725388601, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=444, Percent_Identity=24.5495495495495, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=27.3364485981308, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=30.9734513274336, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24582497, Length=224, Percent_Identity=33.9285714285714, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129315, Length=224, Percent_Identity=33.9285714285714, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 42289; Mature: 42289

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFRFPKIGESGSGGLVVRWLKQVGENIAKDEPVIEVSTDKIATELASPKAGKLMRCLV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCHHHHHHHHH
KEGDEVASGEIIALIDTECAVEEEVVVEEPSPHASCPQDSGKNAAWFSPAVLSLAHREGI
HCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHCCC
SIQQLQQISGTGNDGRVTRRDLENYILEMRQPSCPHIANANENRIPMSPLRRAIASSLSK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
SSDEVPHASLIVDIDVTDLMNLIAEEKDRFFATHGVKLTITSFIIQCLAKALEQFPLLNG
CCCCCCCEEEEEEECHHHHHHHHHHHHHCEEEECCEEEEHHHHHHHHHHHHHHHCCCCCC
SLDGDTIVVKKSINVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKTLADLSARSRANKLDP
CCCCCEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
SETQDGSVTVTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTL
CCCCCCCEEEEECCCCHHHHCCCHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEE
TFDHRVLDGIYGSEFLTSLKNRLESVTMS
EECHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFEFRFPKIGESGSGGLVVRWLKQVGENIAKDEPVIEVSTDKIATELASPKAGKLMRCLV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCHHHHHHHHH
KEGDEVASGEIIALIDTECAVEEEVVVEEPSPHASCPQDSGKNAAWFSPAVLSLAHREGI
HCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHCCC
SIQQLQQISGTGNDGRVTRRDLENYILEMRQPSCPHIANANENRIPMSPLRRAIASSLSK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
SSDEVPHASLIVDIDVTDLMNLIAEEKDRFFATHGVKLTITSFIIQCLAKALEQFPLLNG
CCCCCCCEEEEEEECHHHHHHHHHHHHHCEEEECCEEEEHHHHHHHHHHHHHHHCCCCCC
SLDGDTIVVKKSINVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKTLADLSARSRANKLDP
CCCCCEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
SETQDGSVTVTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTL
CCCCCCCEEEEECCCCHHHHCCCHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEE
TFDHRVLDGIYGSEFLTSLKNRLESVTMS
EECHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]