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The map label for this gene is appA [H]

Identifier: 62184785

GI number: 62184785

Start: 148659

End: 150776

Strand: Direct

Name: appA [H]

Synonym: CAB140

Alternate gene names: 62184785

Gene position: 148659-150776 (Clockwise)

Preceding gene: 62184784

Following gene: 62184786

Centisome position: 12.99

GC content: 40.98

Gene sequence:

>2118_bases
ATGAATAAACGGTGTGTAATAGACAAAATTTTAAAGTGTGTAGTCGTTGGCTCTTTGGTTTTGTTATACTGGTCGTCTGA
TTTGCTTGAAAAAGACATCAAGTTAATCAAAATGAATGTTCGAGATGTACAAGAAGACATCCAAGAATTACTCAGCATCG
TGAAGCAAAATCATGCTACGCGATCTCTAAATCCATCATGTTCTTCTTCCTTATCAGCAACTACGTGTTCTGCTTTTGTA
GAAGTCGGAGATCCGCGTTATCCTAATCTACTTTCTCCAGACCCTTACATGGAAACAACTTTAACGGAACTTATCGGAGA
GGATTTTGTTCCTAAAGGCGTTTTACGTACCGCTCATGTGGGGAAACCAGACAATTTGAGCCCTTTTAACGGGTATGATT
ATGTCGTAAAAATGTATGATTTGTGTGTTCCTGGGTTAGCCAATACTCATGTAGGGAAACACGAAGAATTTGCCCCCGGT
CTGGCCTTAAAAATAGAAGAACGCATGGCCTCAGACGACTCTGGTGATCGTGAGTTTCATATTTATTTACGTCCTAATGT
ATTTTGGGTCCCCGTTGATCCCTTGCGTTTCCCTAAACATGTACAGCTAGCCGAGCATTTTATGCAGCCACATCCTGTCA
CGGCTTATGATTTCAAATTTTATTACGATGCCGTGATGAACCCGTATATTGCAGAGATGCGCGCCGTAGCTTTGCGTTCA
TACCTCGAAGATATCGTCTCTATCCAAGTAGAAAATGATTTAAAATTTATAGTGCGCTGGAAAGCTCATACTCTTGTAAA
TGAAGAGGGCAAAGAGGAGAAGAAAGTGCTCTACTCCGCTTTCTTTAATACTTTATCTTTGAAACCCCTTCCACGTTTCG
TTTATCAGTATTTTGCTAATGGAGAAAAGATCATTAAAAATGACTCTGATCCTGACACCTATCGTAAAGATTCGGTATGG
GCGCAAAACTTCTCCTCACACTGGTCTATGAACTATATTGTGAGCTGCGGCGCATTTTATTTTTCTGGAATGGATGATGA
AAAACTGATCTTCACGAGGAATCCTCACCACTATAACCCTAAAGAAGCTCTAATTGAGAAGCGTTATGTTTATATTAAAG
ATAACTCAGACTCACTATTTCAGGACTTCAAATCAGGGAAACTAGACTTGGCTTATTTACCACCAAATCACGTGGATAAT
CTCGTGAGTTTCATGAAAACTCCGGCCTATAAAAATCAAGCTTCCCGAGGCGAAGCTATTCGTGAGATGATCTACCCAGA
TCGTTCGTACGCGTACATTGGTTGGAATTGTTACTCATTATTTTTTGAGAACCGTCAAGTGCGACGCGCTATGAATATGC
TCATAGATCGTGATAGGATTATAGAAGAGTGTTTAGATGGTCGTGCGCACGTCATTAGCGGGCCCTTCTCGCCTTTTTCT
CCTGCCTATAATCAAAAGATCGAAGGCTGGCATTATTCTCCAGAAGAGGCTGCGCGTATTTTAGAAGAAGAGGGATGGAT
AGATGTTGACGGCGATGGTATCCGAGAAAAAGTCATAGATGGCGTGGTGATTCCTTTTCGTTTCCGCCTTTGTTACTATG
TCAAAAGTGTCACCGGCCGTACGATTGCAGAATATGTAGCTACCGTGTGTAAAGAGATAGGTATTGAGTGTAGTTTATTA
GGATTAGATACCGCTGACCTATCCCAAGCATTTGAAGAAAAGAATTTCGATGCTTTGCTTACAGGCTGGTGTCTAGGATC
GCCTCCCGAGGATCCTCGAGCCCTTTGGCATTCGGAAGGGGCAATGGAAAAAGGCTCAGCTAATGTCGTTGGTTTCCATA
ATCCCGAAGCTGATAAAATTATAGATCAACTTAGCTATGAATATGACGCCAATAAACGCGTAGATCTATACCATCGTTTT
CATGAAGTTATTCATGAAGAATCTCCTTATGCTTTCTTGTACTCCCGTACTTTTTCTTTACTCTATAAAGATTACGTGAA
AAACGTCTTTGTCCCCAAACAGAGAACAGATTTAATTCCTGACGCTCAAGACGAAATGGTGAATCTTCATATGGTTTGGT
TAGACAGGAAGGAGGAAGAGTGCTTAAGTATATCTTAA

Upstream 100 bases:

>100_bases
AATAGCCGGGTGATGGAGTCTTGAAAAGCTTAAAGGCTGAAAGACATGTTTTTCAGGAACTGTCAGAGTGCTGTAGTCTT
TGGCAGAGGATCTGTTTTAT

Downstream 100 bases:

>100_bases
AACGTCTGATATTAATTCCTCTGACGCTGTTTGCCATTATTTCCATTAATTTCATTATATTGAACGCAGCTCCAGGAGAT
GTTATCGAAGAGCAATCCGT

Product: putative transport protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MNKRCVIDKILKCVVVGSLVLLYWSSDLLEKDIKLIKMNVRDVQEDIQELLSIVKQNHATRSLNPSCSSSLSATTCSAFV
EVGDPRYPNLLSPDPYMETTLTELIGEDFVPKGVLRTAHVGKPDNLSPFNGYDYVVKMYDLCVPGLANTHVGKHEEFAPG
LALKIEERMASDDSGDREFHIYLRPNVFWVPVDPLRFPKHVQLAEHFMQPHPVTAYDFKFYYDAVMNPYIAEMRAVALRS
YLEDIVSIQVENDLKFIVRWKAHTLVNEEGKEEKKVLYSAFFNTLSLKPLPRFVYQYFANGEKIIKNDSDPDTYRKDSVW
AQNFSSHWSMNYIVSCGAFYFSGMDDEKLIFTRNPHHYNPKEALIEKRYVYIKDNSDSLFQDFKSGKLDLAYLPPNHVDN
LVSFMKTPAYKNQASRGEAIREMIYPDRSYAYIGWNCYSLFFENRQVRRAMNMLIDRDRIIEECLDGRAHVISGPFSPFS
PAYNQKIEGWHYSPEEAARILEEEGWIDVDGDGIREKVIDGVVIPFRFRLCYYVKSVTGRTIAEYVATVCKEIGIECSLL
GLDTADLSQAFEEKNFDALLTGWCLGSPPEDPRALWHSEGAMEKGSANVVGFHNPEADKIIDQLSYEYDANKRVDLYHRF
HEVIHEESPYAFLYSRTFSLLYKDYVKNVFVPKQRTDLIPDAQDEMVNLHMVWLDRKEEECLSIS

Sequences:

>Translated_705_residues
MNKRCVIDKILKCVVVGSLVLLYWSSDLLEKDIKLIKMNVRDVQEDIQELLSIVKQNHATRSLNPSCSSSLSATTCSAFV
EVGDPRYPNLLSPDPYMETTLTELIGEDFVPKGVLRTAHVGKPDNLSPFNGYDYVVKMYDLCVPGLANTHVGKHEEFAPG
LALKIEERMASDDSGDREFHIYLRPNVFWVPVDPLRFPKHVQLAEHFMQPHPVTAYDFKFYYDAVMNPYIAEMRAVALRS
YLEDIVSIQVENDLKFIVRWKAHTLVNEEGKEEKKVLYSAFFNTLSLKPLPRFVYQYFANGEKIIKNDSDPDTYRKDSVW
AQNFSSHWSMNYIVSCGAFYFSGMDDEKLIFTRNPHHYNPKEALIEKRYVYIKDNSDSLFQDFKSGKLDLAYLPPNHVDN
LVSFMKTPAYKNQASRGEAIREMIYPDRSYAYIGWNCYSLFFENRQVRRAMNMLIDRDRIIEECLDGRAHVISGPFSPFS
PAYNQKIEGWHYSPEEAARILEEEGWIDVDGDGIREKVIDGVVIPFRFRLCYYVKSVTGRTIAEYVATVCKEIGIECSLL
GLDTADLSQAFEEKNFDALLTGWCLGSPPEDPRALWHSEGAMEKGSANVVGFHNPEADKIIDQLSYEYDANKRVDLYHRF
HEVIHEESPYAFLYSRTFSLLYKDYVKNVFVPKQRTDLIPDAQDEMVNLHMVWLDRKEEECLSIS
>Mature_705_residues
MNKRCVIDKILKCVVVGSLVLLYWSSDLLEKDIKLIKMNVRDVQEDIQELLSIVKQNHATRSLNPSCSSSLSATTCSAFV
EVGDPRYPNLLSPDPYMETTLTELIGEDFVPKGVLRTAHVGKPDNLSPFNGYDYVVKMYDLCVPGLANTHVGKHEEFAPG
LALKIEERMASDDSGDREFHIYLRPNVFWVPVDPLRFPKHVQLAEHFMQPHPVTAYDFKFYYDAVMNPYIAEMRAVALRS
YLEDIVSIQVENDLKFIVRWKAHTLVNEEGKEEKKVLYSAFFNTLSLKPLPRFVYQYFANGEKIIKNDSDPDTYRKDSVW
AQNFSSHWSMNYIVSCGAFYFSGMDDEKLIFTRNPHHYNPKEALIEKRYVYIKDNSDSLFQDFKSGKLDLAYLPPNHVDN
LVSFMKTPAYKNQASRGEAIREMIYPDRSYAYIGWNCYSLFFENRQVRRAMNMLIDRDRIIEECLDGRAHVISGPFSPFS
PAYNQKIEGWHYSPEEAARILEEEGWIDVDGDGIREKVIDGVVIPFRFRLCYYVKSVTGRTIAEYVATVCKEIGIECSLL
GLDTADLSQAFEEKNFDALLTGWCLGSPPEDPRALWHSEGAMEKGSANVVGFHNPEADKIIDQLSYEYDANKRVDLYHRF
HEVIHEESPYAFLYSRTFSLLYKDYVKNVFVPKQRTDLIPDAQDEMVNLHMVWLDRKEEECLSIS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=348, Percent_Identity=24.7126436781609, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 81369; Mature: 81369

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRCVIDKILKCVVVGSLVLLYWSSDLLEKDIKLIKMNVRDVQEDIQELLSIVKQNHAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RSLNPSCSSSLSATTCSAFVEVGDPRYPNLLSPDPYMETTLTELIGEDFVPKGVLRTAHV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCC
GKPDNLSPFNGYDYVVKMYDLCVPGLANTHVGKHEEFAPGLALKIEERMASDDSGDREFH
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEE
IYLRPNVFWVPVDPLRFPKHVQLAEHFMQPHPVTAYDFKFYYDAVMNPYIAEMRAVALRS
EEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHH
YLEDIVSIQVENDLKFIVRWKAHTLVNEEGKEEKKVLYSAFFNTLSLKPLPRFVYQYFAN
HHHHHHEEEECCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GEKIIKNDSDPDTYRKDSVWAQNFSSHWSMNYIVSCGAFYFSGMDDEKLIFTRNPHHYNP
CCEEECCCCCCCCHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCH
KEALIEKRYVYIKDNSDSLFQDFKSGKLDLAYLPPNHVDNLVSFMKTPAYKNQASRGEAI
HHHHHHCCEEEEECCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHH
REMIYPDRSYAYIGWNCYSLFFENRQVRRAMNMLIDRDRIIEECLDGRAHVISGPFSPFS
HHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PAYNQKIEGWHYSPEEAARILEEEGWIDVDGDGIREKVIDGVVIPFRFRLCYYVKSVTGR
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHCCEEHHHHHHHHHHHHHHCCH
TIAEYVATVCKEIGIECSLLGLDTADLSQAFEEKNFDALLTGWCLGSPPEDPRALWHSEG
HHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCC
AMEKGSANVVGFHNPEADKIIDQLSYEYDANKRVDLYHRFHEVIHEESPYAFLYSRTFSL
CCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
LYKDYVKNVFVPKQRTDLIPDAQDEMVNLHMVWLDRKEEECLSIS
HHHHHHHHCCCCCHHCCCCCCCCHHHEEEEEEEEECCHHHHCCCC
>Mature Secondary Structure
MNKRCVIDKILKCVVVGSLVLLYWSSDLLEKDIKLIKMNVRDVQEDIQELLSIVKQNHAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RSLNPSCSSSLSATTCSAFVEVGDPRYPNLLSPDPYMETTLTELIGEDFVPKGVLRTAHV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCC
GKPDNLSPFNGYDYVVKMYDLCVPGLANTHVGKHEEFAPGLALKIEERMASDDSGDREFH
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEE
IYLRPNVFWVPVDPLRFPKHVQLAEHFMQPHPVTAYDFKFYYDAVMNPYIAEMRAVALRS
EEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHH
YLEDIVSIQVENDLKFIVRWKAHTLVNEEGKEEKKVLYSAFFNTLSLKPLPRFVYQYFAN
HHHHHHEEEECCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GEKIIKNDSDPDTYRKDSVWAQNFSSHWSMNYIVSCGAFYFSGMDDEKLIFTRNPHHYNP
CCEEECCCCCCCCHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCH
KEALIEKRYVYIKDNSDSLFQDFKSGKLDLAYLPPNHVDNLVSFMKTPAYKNQASRGEAI
HHHHHHCCEEEEECCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHH
REMIYPDRSYAYIGWNCYSLFFENRQVRRAMNMLIDRDRIIEECLDGRAHVISGPFSPFS
HHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PAYNQKIEGWHYSPEEAARILEEEGWIDVDGDGIREKVIDGVVIPFRFRLCYYVKSVTGR
CCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHCCEEHHHHHHHHHHHHHHCCH
TIAEYVATVCKEIGIECSLLGLDTADLSQAFEEKNFDALLTGWCLGSPPEDPRALWHSEG
HHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCC
AMEKGSANVVGFHNPEADKIIDQLSYEYDANKRVDLYHRFHEVIHEESPYAFLYSRTFSL
CCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
LYKDYVKNVFVPKQRTDLIPDAQDEMVNLHMVWLDRKEEECLSIS
HHHHHHHHCCCCCHHCCCCCCCCHHHEEEEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]