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The map label for this gene is aspS

Identifier: 62184723

GI number: 62184723

Start: 95118

End: 96872

Strand: Direct

Name: aspS

Synonym: CAB079

Alternate gene names: 62184723

Gene position: 95118-96872 (Clockwise)

Preceding gene: 62184722

Following gene: 62184724

Centisome position: 8.31

GC content: 40.0

Gene sequence:

>1755_bases
ATGAAATACAGAACACATCGTTGTAATGAATTATCCCTAAGCAATGTAGGAGAACGTGTCCGTTTATCTGGATGGGTACA
TCGTTATCGTAATCACGGGGGAGTCGTTTTTATAGACTTACGAGATCGTTTTGGAATCACACAGATCGTGTGTCGTGAGG
ACGAAAAACCTGAATTACACCAGTTAGTGGATTCTGTACGTTCGGAGTGGGTTTTATCCATAGAGGGAACGGTATGCCGA
CGTTTGGAAGGCATGGAAAATGCGAACTTAGCCACAGGGGAGATTGAGGTAGATATTGAAAAAGTCGATATCTTATCGAA
AGCCAAAAACCTCCCTTTTTCTATATCAGATGATCATATTCACGTTAATGAAGAATTGCGCCTAGAGTATCGTTATCTTG
ATATGCGTAGAGGTCAAATTTTAGATCGATTGGTCCATCGTCATAAAGTGATGATGGCTTGTCGTCAGTATATGGATAAA
CAGGGTTTTACCGAGGTAGTTACGCCAATTTTAGGTAAATCTACTCCCGAAGGAGCTCGGGATTATCTTGTCCCTTCTAG
AATATATCCGGGAAGTTTTTATGCTTTGCCACAGTCGCCACAATTGTTTAAACAAATTCTTATGGTGGGAGGGCTCGACC
GGTATTTCCAAATAGCCACGTGTTTTAGAGATGAAGATCTTCGAGCCGACCGTCAGCCTGAATTTGCTCAGATTGATATT
GAAATGAGTTTCGGTACCCCCAATGATCTTTTCCCTATCATTGAACAGTTAGTAGTAGAGATGTTTGCTGTTCAAGGGAT
TAAAATAGATCTTCCCTTGCCGAGAATGACCTATCAAGAAGCTAAAGATTTATACGGTACAGATAAACCAGATCTTCGAT
TTGGTTTGCAGCTTCATGATTGTCGGGAACATGCCAAAGAGTTTTCATTCTCCATATTTTTAGATCAGCTTGCTCAAGGA
GGCACAATAAAAGGTTTTTGTGTGCCTGGCGGAGCGGATATGTCTCGTAAGCAGCTGGATGTTTATACAGAATTTGTTAA
GCGTTACGGTGCTATGGGGTTGGTTTGGATTAAAAAACAAGAGAGTGGCATCGCTTCTAATGTAGCGAAATTTGCTTCTG
AAGCTGTGTTCCAAGCCATGTTTGCTGATTTCGGAGCTCAAAATAATGATATCCTACTGTTGATAGCAGCTCCAGAAGAT
GTGGCCAATCAATCTTTAGATCATTTGCGTAGATTGATAGCTAAAGAGCGCAACTTCTACAATGAAGCGCAATACAACTT
TGTTTGGATTACAGATTTTCCTTTATTTGCTAAAGAAGATGGGAAAATATGCTCAGAGCATCATCCATTCACCTCACCCT
TAGATGAGGATATTCCTTTATTGGATAAAGATCCTTTATCGGTACGATCTTCCAGTTATGACTTAGTTCTTAACGGATAC
GAAATCGCTTCAGGGTCACAACGTATTCATAACGCAGATTTGCAAAATAAAATATTTTCTATTTTAGAGTTATCACCAGA
GAGTATAAAAGAAAAGTTCGACTTCTTTATCGATGCCCTAAGTTTTGGGACGCCGCCACATTTAGGAATTGCTTTAGGTT
TAGATCGTATTATGATGGTATTGACGGGGGCAGAAGGTATTAGAGAAGTCATAGCTTTCCCAAAAACTCAGAAAGCCGCG
GATTTAATGATGGATGCTCCTGCGGAAATTATGACTTCCCAATTAAAAGAGCTAAGTATTAAGGTAACTTCTTAA

Upstream 100 bases:

>100_bases
GGAGAAAGAGAGTTGGTTTCAGAGCAATTGACAGTGAAGAATATGTCTTTGCGCCAAGAGTTCTCTGGTTCAAAGCAAGA
GGTAGAGCAAAGGTTGCTAT

Downstream 100 bases:

>100_bases
ATAATTTTATACCTATTTTAGGTGCATGGCGCCATACCATGTGAGTGTCTAGGCTACAACCATAGGAGAGAAAATATGAA
AAAACAATGGTATTTAATTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKYRTHRCNELSLSNVGERVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEKPELHQLVDSVRSEWVLSIEGTVCR
RLEGMENANLATGEIEVDIEKVDILSKAKNLPFSISDDHIHVNEELRLEYRYLDMRRGQILDRLVHRHKVMMACRQYMDK
QGFTEVVTPILGKSTPEGARDYLVPSRIYPGSFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGTPNDLFPIIEQLVVEMFAVQGIKIDLPLPRMTYQEAKDLYGTDKPDLRFGLQLHDCREHAKEFSFSIFLDQLAQG
GTIKGFCVPGGADMSRKQLDVYTEFVKRYGAMGLVWIKKQESGIASNVAKFASEAVFQAMFADFGAQNNDILLLIAAPED
VANQSLDHLRRLIAKERNFYNEAQYNFVWITDFPLFAKEDGKICSEHHPFTSPLDEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNADLQNKIFSILELSPESIKEKFDFFIDALSFGTPPHLGIALGLDRIMMVLTGAEGIREVIAFPKTQKAA
DLMMDAPAEIMTSQLKELSIKVTS

Sequences:

>Translated_584_residues
MKYRTHRCNELSLSNVGERVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEKPELHQLVDSVRSEWVLSIEGTVCR
RLEGMENANLATGEIEVDIEKVDILSKAKNLPFSISDDHIHVNEELRLEYRYLDMRRGQILDRLVHRHKVMMACRQYMDK
QGFTEVVTPILGKSTPEGARDYLVPSRIYPGSFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGTPNDLFPIIEQLVVEMFAVQGIKIDLPLPRMTYQEAKDLYGTDKPDLRFGLQLHDCREHAKEFSFSIFLDQLAQG
GTIKGFCVPGGADMSRKQLDVYTEFVKRYGAMGLVWIKKQESGIASNVAKFASEAVFQAMFADFGAQNNDILLLIAAPED
VANQSLDHLRRLIAKERNFYNEAQYNFVWITDFPLFAKEDGKICSEHHPFTSPLDEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNADLQNKIFSILELSPESIKEKFDFFIDALSFGTPPHLGIALGLDRIMMVLTGAEGIREVIAFPKTQKAA
DLMMDAPAEIMTSQLKELSIKVTS
>Mature_584_residues
MKYRTHRCNELSLSNVGERVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEKPELHQLVDSVRSEWVLSIEGTVCR
RLEGMENANLATGEIEVDIEKVDILSKAKNLPFSISDDHIHVNEELRLEYRYLDMRRGQILDRLVHRHKVMMACRQYMDK
QGFTEVVTPILGKSTPEGARDYLVPSRIYPGSFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGTPNDLFPIIEQLVVEMFAVQGIKIDLPLPRMTYQEAKDLYGTDKPDLRFGLQLHDCREHAKEFSFSIFLDQLAQG
GTIKGFCVPGGADMSRKQLDVYTEFVKRYGAMGLVWIKKQESGIASNVAKFASEAVFQAMFADFGAQNNDILLLIAAPED
VANQSLDHLRRLIAKERNFYNEAQYNFVWITDFPLFAKEDGKICSEHHPFTSPLDEDIPLLDKDPLSVRSSSYDLVLNGY
EIASGSQRIHNADLQNKIFSILELSPESIKEKFDFFIDALSFGTPPHLGIALGLDRIMMVLTGAEGIREVIAFPKTQKAA
DLMMDAPAEIMTSQLKELSIKVTS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=593, Percent_Identity=40.3035413153457, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI45439306, Length=256, Percent_Identity=26.171875, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=24.5059288537549, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=24.5059288537549, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=45.9183673469388, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1790571, Length=255, Percent_Identity=27.843137254902, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1789256, Length=258, Percent_Identity=27.1317829457364, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=597, Percent_Identity=36.3484087102178, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=25.1798561151079, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=252, Percent_Identity=25.7936507936508, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=252, Percent_Identity=25.7936507936508, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=258, Percent_Identity=25.5813953488372, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=317, Percent_Identity=36.9085173501577, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321807, Length=255, Percent_Identity=27.843137254902, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=25.3846153846154, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323011, Length=304, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=35.7737104825291, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI17136276, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640849, Length=275, Percent_Identity=27.6363636363636, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640851, Length=275, Percent_Identity=27.6363636363636, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CHLAB (Q5L734)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219508.1
- ProteinModelPortal:   Q5L734
- SMR:   Q5L734
- GeneID:   3337265
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB079
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q5L734
- ProtClustDB:   PRK00476
- BioCyc:   CABO218497:CAB079-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66483; Mature: 66483

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00527 RIBOSOMAL_S14

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRTHRCNELSLSNVGERVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEKPELH
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCHHH
QLVDSVRSEWVLSIEGTVCRRLEGMENANLATGEIEVDIEKVDILSKAKNLPFSISDDHI
HHHHHHHHCEEEEECHHHHHHHCCCCCCCEECCEEEEEHHHHHHHHHHCCCCEECCCCEE
HVNEELRLEYRYLDMRRGQILDRLVHRHKVMMACRQYMDKQGFTEVVTPILGKSTPEGAR
EECHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DYLVPSRIYPGSFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFGTPNDLFPIIEQLVVEMFAVQGIKIDLPLPRMTYQEAKDLYGTDKPDLRFGLQLHD
EEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCEECCCHHH
CREHAKEFSFSIFLDQLAQGGTIKGFCVPGGADMSRKQLDVYTEFVKRYGAMGLVWIKKQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
ESGIASNVAKFASEAVFQAMFADFGAQNNDILLLIAAPEDVANQSLDHLRRLIAKERNFY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
NEAQYNFVWITDFPLFAKEDGKICSEHHPFTSPLDEDIPLLDKDPLSVRSSSYDLVLNGY
CCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECE
EIASGSQRIHNADLQNKIFSILELSPESIKEKFDFFIDALSFGTPPHLGIALGLDRIMMV
EECCCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTGAEGIREVIAFPKTQKAADLMMDAPAEIMTSQLKELSIKVTS
HHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MKYRTHRCNELSLSNVGERVRLSGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEKPELH
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCHHH
QLVDSVRSEWVLSIEGTVCRRLEGMENANLATGEIEVDIEKVDILSKAKNLPFSISDDHI
HHHHHHHHCEEEEECHHHHHHHCCCCCCCEECCEEEEEHHHHHHHHHHCCCCEECCCCEE
HVNEELRLEYRYLDMRRGQILDRLVHRHKVMMACRQYMDKQGFTEVVTPILGKSTPEGAR
EECHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DYLVPSRIYPGSFYALPQSPQLFKQILMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFGTPNDLFPIIEQLVVEMFAVQGIKIDLPLPRMTYQEAKDLYGTDKPDLRFGLQLHD
EEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCEECCCHHH
CREHAKEFSFSIFLDQLAQGGTIKGFCVPGGADMSRKQLDVYTEFVKRYGAMGLVWIKKQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
ESGIASNVAKFASEAVFQAMFADFGAQNNDILLLIAAPEDVANQSLDHLRRLIAKERNFY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
NEAQYNFVWITDFPLFAKEDGKICSEHHPFTSPLDEDIPLLDKDPLSVRSSSYDLVLNGY
CCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECE
EIASGSQRIHNADLQNKIFSILELSPESIKEKFDFFIDALSFGTPPHLGIALGLDRIMMV
EECCCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTGAEGIREVIAFPKTQKAADLMMDAPAEIMTSQLKELSIKVTS
HHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA