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The map label for this gene is hisS

Identifier: 62184722

GI number: 62184722

Start: 93851

End: 95143

Strand: Direct

Name: hisS

Synonym: CAB078

Alternate gene names: 62184722

Gene position: 93851-95143 (Clockwise)

Preceding gene: 62184721

Following gene: 62184723

Centisome position: 8.2

GC content: 42.23

Gene sequence:

>1293_bases
GTGAAAGTAGCTTTGCCCAAAGGGGTGTTCGATATATTCCCTTACATTACGGATGCGAAACATATGTGGAGACACACTTC
TCTATGGCATCGGGTCGAGGATGTGATTCATGACGTATGTGGTCTATACGGGTTTTCAGAAATTCGTACGCCTGTTTTCG
AAAAGTCGGAAGTGTTTTTGCATGTCGGGGAACAAAGTGACATTGTAAAAAAAGAAATGTATACCTTTCTGGATAAGAAA
GGGCGGTCATTGACACTGCGTCCTGAAGGAACCGCTCCCATAGTGCGCTCGTTCATAGATAATTCTATGAACCAACGTGA
TGACAATAAATTTTATTATATTCTGCCTATGTTCCGTTATGAAAGGCAGCAGTCAGGAAGGTACCGTCAGCATCATCAGT
TTGGGGTAGAAGCTATAGGGGTGCGTCATCCTCTCCGTGATGCGGAAATCCTTGCTTTACTTTGGCATTTTTATTCTGCT
GTGGGTTTGCAGCACATGCAAGTGCAATTGAACTTTTTAGGTGGGGAGGTTACACGCAAACGCTACGATAAGATTTTGCG
AGAATACTTCCTTGATCATTTAAGTTCTTTATCTTTGCTAAGTAAAGAACGATTTAATACAAATTTATTGAGGATATTGG
ATTCCAAAGAGCCAGAAGATCAAGAGATTATCCAATCCGCACCCCCGATTCTTGACTATGTTTCTGATGATGATCGTAAA
TATTTTGATGAAATTCTTTCTGCTTTAGATGCTTTAAACATTGCCTATGATATTAACCCCAGGCTTGTGCGTGGTTTAGA
TTACTATACGGATCTAGTTTTTGAAGCGATCACAACATGTAGAGATCATTCCTATGCTTTGGGAGGAGGAGGGCGCTATG
ACGGCCTGATTGCTTCTTCTGGAGGGCCAGCCACTCCCGCTTGTGGTTTTGGCATTGGTTTAGAGAGGGTGATTCAGACT
TTATTGGCGCAAGGGAATTTCACACCGCTATCCTCTCACAAATTGCGTTTGATCCCGGTGGAGTCGCAGGCCGACTCTTT
TTGTTTTGTTTGGGCACAGCATTTACGTAGTTTAGGAATCCCTACAGAAGTGGATTGGACGCATAAAAAATTAAAAAATG
CTTTAAAAATAGCAGATGCGGAGAAAGCCACTTTTGTCTGTCCCGTGGGAGAAAGAGAGTTGGTTTCAGAGCAATTGACA
GTGAAGAATATGTCTTTGCGCCAAGAGTTCTCTGGTTCAAAGCAAGAGGTAGAGCAAAGGTTGCTATATGAAATACAGAA
CACATCGTTGTAA

Upstream 100 bases:

>100_bases
GAATCTTTTGACTCTCATATAGGAAGGGTTTAATTTTGCTCTTTATCCGAATTTTGGATATTCTACTCTTAAAGTTGTTC
AATTAAATTAAAGACATACG

Downstream 100 bases:

>100_bases
TGAATTATCCCTAAGCAATGTAGGAGAACGTGTCCGTTTATCTGGATGGGTACATCGTTATCGTAATCACGGGGGAGTCG
TTTTTATAGACTTACGAGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKVALPKGVFDIFPYITDAKHMWRHTSLWHRVEDVIHDVCGLYGFSEIRTPVFEKSEVFLHVGEQSDIVKKEMYTFLDKK
GRSLTLRPEGTAPIVRSFIDNSMNQRDDNKFYYILPMFRYERQQSGRYRQHHQFGVEAIGVRHPLRDAEILALLWHFYSA
VGLQHMQVQLNFLGGEVTRKRYDKILREYFLDHLSSLSLLSKERFNTNLLRILDSKEPEDQEIIQSAPPILDYVSDDDRK
YFDEILSALDALNIAYDINPRLVRGLDYYTDLVFEAITTCRDHSYALGGGGRYDGLIASSGGPATPACGFGIGLERVIQT
LLAQGNFTPLSSHKLRLIPVESQADSFCFVWAQHLRSLGIPTEVDWTHKKLKNALKIADAEKATFVCPVGERELVSEQLT
VKNMSLRQEFSGSKQEVEQRLLYEIQNTSL

Sequences:

>Translated_430_residues
MKVALPKGVFDIFPYITDAKHMWRHTSLWHRVEDVIHDVCGLYGFSEIRTPVFEKSEVFLHVGEQSDIVKKEMYTFLDKK
GRSLTLRPEGTAPIVRSFIDNSMNQRDDNKFYYILPMFRYERQQSGRYRQHHQFGVEAIGVRHPLRDAEILALLWHFYSA
VGLQHMQVQLNFLGGEVTRKRYDKILREYFLDHLSSLSLLSKERFNTNLLRILDSKEPEDQEIIQSAPPILDYVSDDDRK
YFDEILSALDALNIAYDINPRLVRGLDYYTDLVFEAITTCRDHSYALGGGGRYDGLIASSGGPATPACGFGIGLERVIQT
LLAQGNFTPLSSHKLRLIPVESQADSFCFVWAQHLRSLGIPTEVDWTHKKLKNALKIADAEKATFVCPVGERELVSEQLT
VKNMSLRQEFSGSKQEVEQRLLYEIQNTSL
>Mature_430_residues
MKVALPKGVFDIFPYITDAKHMWRHTSLWHRVEDVIHDVCGLYGFSEIRTPVFEKSEVFLHVGEQSDIVKKEMYTFLDKK
GRSLTLRPEGTAPIVRSFIDNSMNQRDDNKFYYILPMFRYERQQSGRYRQHHQFGVEAIGVRHPLRDAEILALLWHFYSA
VGLQHMQVQLNFLGGEVTRKRYDKILREYFLDHLSSLSLLSKERFNTNLLRILDSKEPEDQEIIQSAPPILDYVSDDDRK
YFDEILSALDALNIAYDINPRLVRGLDYYTDLVFEAITTCRDHSYALGGGGRYDGLIASSGGPATPACGFGIGLERVIQT
LLAQGNFTPLSSHKLRLIPVESQADSFCFVWAQHLRSLGIPTEVDWTHKKLKNALKIADAEKATFVCPVGERELVSEQLT
VKNMSLRQEFSGSKQEVEQRLLYEIQNTSL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=402, Percent_Identity=38.3084577114428, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CHLAB (Q5L735)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219507.1
- ProteinModelPortal:   Q5L735
- SMR:   Q5L735
- GeneID:   3337996
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB078
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q5L735
- ProtClustDB:   PRK00037
- BioCyc:   CABO218497:CAB078-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 49387; Mature: 49387

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALPKGVFDIFPYITDAKHMWRHTSLWHRVEDVIHDVCGLYGFSEIRTPVFEKSEVFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEE
HVGEQSDIVKKEMYTFLDKKGRSLTLRPEGTAPIVRSFIDNSMNQRDDNKFYYILPMFRY
EECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
ERQQSGRYRQHHQFGVEAIGVRHPLRDAEILALLWHFYSAVGLQHMQVQLNFLGGEVTRK
HHHHCCCHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEHHCCHHHHH
RYDKILREYFLDHLSSLSLLSKERFNTNLLRILDSKEPEDQEIIQSAPPILDYVSDDDRK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCHHHHCCCCHHH
YFDEILSALDALNIAYDINPRLVRGLDYYTDLVFEAITTCRDHSYALGGGGRYDGLIASS
HHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECC
GGPATPACGFGIGLERVIQTLLAQGNFTPLSSHKLRLIPVESQADSFCFVWAQHLRSLGI
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
PTEVDWTHKKLKNALKIADAEKATFVCPVGERELVSEQLTVKNMSLRQEFSGSKQEVEQR
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTSL
HHHHHHCCCC
>Mature Secondary Structure
MKVALPKGVFDIFPYITDAKHMWRHTSLWHRVEDVIHDVCGLYGFSEIRTPVFEKSEVFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEE
HVGEQSDIVKKEMYTFLDKKGRSLTLRPEGTAPIVRSFIDNSMNQRDDNKFYYILPMFRY
EECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
ERQQSGRYRQHHQFGVEAIGVRHPLRDAEILALLWHFYSAVGLQHMQVQLNFLGGEVTRK
HHHHCCCHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEHHCCHHHHH
RYDKILREYFLDHLSSLSLLSKERFNTNLLRILDSKEPEDQEIIQSAPPILDYVSDDDRK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCHHHHCCCCHHH
YFDEILSALDALNIAYDINPRLVRGLDYYTDLVFEAITTCRDHSYALGGGGRYDGLIASS
HHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECC
GGPATPACGFGIGLERVIQTLLAQGNFTPLSSHKLRLIPVESQADSFCFVWAQHLRSLGI
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
PTEVDWTHKKLKNALKIADAEKATFVCPVGERELVSEQLTVKNMSLRQEFSGSKQEVEQR
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLYEIQNTSL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA