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The map label for this gene is ruvC [H]

Identifier: 161501898

GI number: 161501898

Start: 123997

End: 124503

Strand: Direct

Name: ruvC [H]

Synonym: CAB118

Alternate gene names: 161501898

Gene position: 123997-124503 (Clockwise)

Preceding gene: 62184762

Following gene: 161501897

Centisome position: 10.84

GC content: 39.64

Gene sequence:

>507_bases
ATGACGCAATTGATTATGGGGATAGATCCTGGAACTTTAGTTTCTGGATATGCAATTATCCTTGTTGAGCAGCGTTACAA
GATCCGAGCTCATAGCTACGGGGCGATTCGATTATCTTCCAAAGATTCATTAACACAACGTTATAAGCAGCTTTTCCAAA
CATTATCTGGAGTATTAGATAATGTTACTCCCGATGCTGTTGTGCTTGAAACCCAATATGTTCATAAGAATCCTCAAAGT
GCTATAAAGTTAGGCATGGGACGAGGAGTCCTCGTTTTAGCTGCTGCTTTACGAGATATACCCGTCTTTGAATATACTCC
CAATGTAGCCAAGAGAGCTGTTGTAGGTAAGGGAAATGCAAGTAAACAGCAAGTGCAACTTATGGTGAGTAAGATTTTAA
ATATTCCTGATGTTTTAAATTCGGATTGTGAGGATATTGCAGATGCATTTGCATTAGCGATATGCCACGCGCATACTTCT
GCATATACTTGTTTGGGAGTTCGATAA

Upstream 100 bases:

>100_bases
ACCTAAGAATTTCTAGTTGTAATTATATAGAACTCACATAGAATAAACCCTACACATCCCATTAGGGGAACTTTTTAGAA
GCCTGTACACGGATTTGTTT

Downstream 100 bases:

>100_bases
TGTACGACTATATTCGTGGTGTGCTTACTTATATCAGTTCAAGCACTATGGTGATTGAAAGTCAGGGATTGGGATTTAGT
ATTTTTGCTCCTGAGAGATG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTQLIMGIDPGTLVSGYAIILVEQRYKIRAHSYGAIRLSSKDSLTQRYKQLFQTLSGVLDNVTPDAVVLETQYVHKNPQS
AIKLGMGRGVLVLAAALRDIPVFEYTPNVAKRAVVGKGNASKQQVQLMVSKILNIPDVLNSDCEDIADAFALAICHAHTS
AYTCLGVR

Sequences:

>Translated_168_residues
MTQLIMGIDPGTLVSGYAIILVEQRYKIRAHSYGAIRLSSKDSLTQRYKQLFQTLSGVLDNVTPDAVVLETQYVHKNPQS
AIKLGMGRGVLVLAAALRDIPVFEYTPNVAKRAVVGKGNASKQQVQLMVSKILNIPDVLNSDCEDIADAFALAICHAHTS
AYTCLGVR
>Mature_167_residues
TQLIMGIDPGTLVSGYAIILVEQRYKIRAHSYGAIRLSSKDSLTQRYKQLFQTLSGVLDNVTPDAVVLETQYVHKNPQSA
IKLGMGRGVLVLAAALRDIPVFEYTPNVAKRAVVGKGNASKQQVQLMVSKILNIPDVLNSDCEDIADAFALAICHAHTSA
YTCLGVR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=136, Percent_Identity=40.4411764705882, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18272; Mature: 18141

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLIMGIDPGTLVSGYAIILVEQRYKIRAHSYGAIRLSSKDSLTQRYKQLFQTLSGVLD
CCEEEEECCCCCHHCCEEEEEEECCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHH
NVTPDAVVLETQYVHKNPQSAIKLGMGRGVLVLAAALRDIPVFEYTPNVAKRAVVGKGNA
CCCCCEEEEEHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCC
SKQQVQLMVSKILNIPDVLNSDCEDIADAFALAICHAHTSAYTCLGVR
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
TQLIMGIDPGTLVSGYAIILVEQRYKIRAHSYGAIRLSSKDSLTQRYKQLFQTLSGVLD
CEEEEECCCCCHHCCEEEEEEECCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHH
NVTPDAVVLETQYVHKNPQSAIKLGMGRGVLVLAAALRDIPVFEYTPNVAKRAVVGKGNA
CCCCCEEEEEHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCC
SKQQVQLMVSKILNIPDVLNSDCEDIADAFALAICHAHTSAYTCLGVR
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]