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The map label for this gene is eno

Identifier: 28572934

GI number: 28572934

Start: 898644

End: 899930

Strand: Reverse

Name: eno

Synonym: TW793

Alternate gene names: 28572934

Gene position: 899930-898644 (Counterclockwise)

Preceding gene: 28572935

Following gene: 28572933

Centisome position: 97.19

GC content: 50.12

Gene sequence:

>1287_bases
ATGTGTCTAATAGATTCTGTACATGCAAGGCAGATCCTCGACTCTAGAGGCACTCCGACCGTGGAGGTTGAGGTAACTCT
CGAAGATGGATCTATGGGAAGATCCGCAGTTCCATCTGGAGCTTCTACCGGTGCGTTTGAGGCGCACGAATTGCGCGATC
AGGATAATAATGAATACCTCGGTAAGGGCGTCACGCGGGCTGTTCGTTCTGTTAATTCCGAGATAGCACCGGTTCTTATC
GGTTTTGACGCATTCGATCAGGTTGGCCTTGACCACCGTATGATTGAGCTAGATGGGACTAACAACAAGAGTAGGCTTGG
CGCAAATGCCATTCTTGGCGTTAGTTTGGCCTCCGCGAGCGCTGCGGCACGCGCCGCTGACCTGTCGCTTTTTAGGTATT
TAGGCGGTCCATCCTCGCGCATATTGCCGGTTCCAATGATGAATATAATAAACGGCGGTGCGCACGCTGATACAGGTGTT
GATATTCAAGAGTTTATGATTCTGCCGGTCGGCGCACGTAGTTTTTCGGAATCCTTGCGCTGGGGCTGTGAGGTGTATCA
CTCTTTGAAGGTCCAACTTAGAGAGAGCGGACTTTCGTCTGGTTTGGGTGATGAGGGGGGTTTTGCCCCTGCTTTGCGCA
GCAATCGCACGGCGCTGGATTTGATTTTATCTGCTATTGAGAAAGCTGGTTTCTCCCCGGGGATCGATATAGTGCTTGCC
CTTGATATCGCGGCGAGCGAGTTCTGTAAAGCACCTGGACACTACAGATTTGAAGGGAAAGATATAACATCTGACGAGCT
CATTTCATACTATGAGGGCCTGTTATCTTCATATCCGCTTGTCAGCATAGAAGATCCGCTTGATCAAGACGATTGGGAGG
GGTATCGCACCCTAACCACCCACATTGGTGATAGGGTGCAAATTGTTGGCGATGATCTCTTCGTAACTAATACCTCGAGG
TTATCCAGGGGGATTCAGTCTGGTGTGGCAAATTCTATTCTTGTTAAAGTAAATCAAATAGGTACTTTGACCGAGACGTT
TGACGCAGTCGATATGGCAGCTAAAGGGGGATATACCGCAGTTCTGTCTCATCGCTCGGGCGAAACCGAGGATACAACTA
TCGCAGATATGGCGGTTGCAACAAACTGTGGACAGATAAAGACTGGCGCGCCCGCTCGCGGCGAGCGTATTGCAAAGTAC
AACCAGCTCTTGCGAATTGAGGAAAAGCTTGGAAGGTCTGCTCGTTATGCTGGGTGGCTATCGTACCCACGATGGCAAGG
CAAATGA

Upstream 100 bases:

>100_bases
GGGTGCGACTCTCTCAGTACACCCCTCGCGATATACTGAGAGACATGTGTATTCATGCACGGGCTTCTCACGCCGGGTGT
GTACGTGGTGGAAATCTGTC

Downstream 100 bases:

>100_bases
ATAGCGTCAGACGCTCTAATATCAGGGTCTATTTAATTATCCTGATCATTCTTGTCCTCGGCACTCCGGGCATATTTGTT
ACAATCTCCCAGATATATAG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MCLIDSVHARQILDSRGTPTVEVEVTLEDGSMGRSAVPSGASTGAFEAHELRDQDNNEYLGKGVTRAVRSVNSEIAPVLI
GFDAFDQVGLDHRMIELDGTNNKSRLGANAILGVSLASASAAARAADLSLFRYLGGPSSRILPVPMMNIINGGAHADTGV
DIQEFMILPVGARSFSESLRWGCEVYHSLKVQLRESGLSSGLGDEGGFAPALRSNRTALDLILSAIEKAGFSPGIDIVLA
LDIAASEFCKAPGHYRFEGKDITSDELISYYEGLLSSYPLVSIEDPLDQDDWEGYRTLTTHIGDRVQIVGDDLFVTNTSR
LSRGIQSGVANSILVKVNQIGTLTETFDAVDMAAKGGYTAVLSHRSGETEDTTIADMAVATNCGQIKTGAPARGERIAKY
NQLLRIEEKLGRSARYAGWLSYPRWQGK

Sequences:

>Translated_428_residues
MCLIDSVHARQILDSRGTPTVEVEVTLEDGSMGRSAVPSGASTGAFEAHELRDQDNNEYLGKGVTRAVRSVNSEIAPVLI
GFDAFDQVGLDHRMIELDGTNNKSRLGANAILGVSLASASAAARAADLSLFRYLGGPSSRILPVPMMNIINGGAHADTGV
DIQEFMILPVGARSFSESLRWGCEVYHSLKVQLRESGLSSGLGDEGGFAPALRSNRTALDLILSAIEKAGFSPGIDIVLA
LDIAASEFCKAPGHYRFEGKDITSDELISYYEGLLSSYPLVSIEDPLDQDDWEGYRTLTTHIGDRVQIVGDDLFVTNTSR
LSRGIQSGVANSILVKVNQIGTLTETFDAVDMAAKGGYTAVLSHRSGETEDTTIADMAVATNCGQIKTGAPARGERIAKY
NQLLRIEEKLGRSARYAGWLSYPRWQGK
>Mature_428_residues
MCLIDSVHARQILDSRGTPTVEVEVTLEDGSMGRSAVPSGASTGAFEAHELRDQDNNEYLGKGVTRAVRSVNSEIAPVLI
GFDAFDQVGLDHRMIELDGTNNKSRLGANAILGVSLASASAAARAADLSLFRYLGGPSSRILPVPMMNIINGGAHADTGV
DIQEFMILPVGARSFSESLRWGCEVYHSLKVQLRESGLSSGLGDEGGFAPALRSNRTALDLILSAIEKAGFSPGIDIVLA
LDIAASEFCKAPGHYRFEGKDITSDELISYYEGLLSSYPLVSIEDPLDQDDWEGYRTLTTHIGDRVQIVGDDLFVTNTSR
LSRGIQSGVANSILVKVNQIGTLTETFDAVDMAAKGGYTAVLSHRSGETEDTTIADMAVATNCGQIKTGAPARGERIAKY
NQLLRIEEKLGRSARYAGWLSYPRWQGK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=52.4475524475524, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=50.1165501165501, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=49.6503496503497, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=49.6503496503497, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=45.1990632318501, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=22.6865671641791, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=22.6865671641791, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=22.6865671641791, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=57.4117647058823, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=51.8604651162791, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=51.8604651162791, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=48.9473684210526, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.537037037037, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.3055555555556, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.3055555555556, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=49.3055555555556, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50.6976744186047, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50.6976744186047, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50.6976744186047, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50.6976744186047, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50.6976744186047, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50.6976744186047, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_TROW8 (Q83H73)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789714.1
- ProteinModelPortal:   Q83H73
- SMR:   Q83H73
- STRING:   Q83H73
- GeneID:   1064993
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW793
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- PhylomeDB:   Q83H73
- ProtClustDB:   PRK00077
- BioCyc:   TWHI218496:TW0759-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45962; Mature: 45962

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 336-336 BINDING 155-155 BINDING 164-164 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLIDSVHARQILDSRGTPTVEVEVTLEDGSMGRSAVPSGASTGAFEAHELRDQDNNEYL
CCEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHH
GKGVTRAVRSVNSEIAPVLIGFDAFDQVGLDHRMIELDGTNNKSRLGANAILGVSLASAS
HHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHH
AAARAADLSLFRYLGGPSSRILPVPMMNIINGGAHADTGVDIQEFMILPVGARSFSESLR
HHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
WGCEVYHSLKVQLRESGLSSGLGDEGGFAPALRSNRTALDLILSAIEKAGFSPGIDIVLA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEE
LDIAASEFCKAPGHYRFEGKDITSDELISYYEGLLSSYPLVSIEDPLDQDDWEGYRTLTT
EEHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
HIGDRVQIVGDDLFVTNTSRLSRGIQSGVANSILVKVNQIGTLTETFDAVDMAAKGGYTA
HCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEE
VLSHRSGETEDTTIADMAVATNCGQIKTGAPARGERIAKYNQLLRIEEKLGRSARYAGWL
EEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
SYPRWQGK
CCCCCCCC
>Mature Secondary Structure
MCLIDSVHARQILDSRGTPTVEVEVTLEDGSMGRSAVPSGASTGAFEAHELRDQDNNEYL
CCEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHH
GKGVTRAVRSVNSEIAPVLIGFDAFDQVGLDHRMIELDGTNNKSRLGANAILGVSLASAS
HHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHH
AAARAADLSLFRYLGGPSSRILPVPMMNIINGGAHADTGVDIQEFMILPVGARSFSESLR
HHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
WGCEVYHSLKVQLRESGLSSGLGDEGGFAPALRSNRTALDLILSAIEKAGFSPGIDIVLA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEE
LDIAASEFCKAPGHYRFEGKDITSDELISYYEGLLSSYPLVSIEDPLDQDDWEGYRTLTT
EEHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
HIGDRVQIVGDDLFVTNTSRLSRGIQSGVANSILVKVNQIGTLTETFDAVDMAAKGGYTA
HCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEE
VLSHRSGETEDTTIADMAVATNCGQIKTGAPARGERIAKYNQLLRIEEKLGRSARYAGWL
EEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
SYPRWQGK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174