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The map label for this gene is dnaB [H]

Identifier: 28572893

GI number: 28572893

Start: 849132

End: 850457

Strand: Direct

Name: dnaB [H]

Synonym: TW752

Alternate gene names: 28572893

Gene position: 849132-850457 (Clockwise)

Preceding gene: 28572892

Following gene: 28572894

Centisome position: 91.71

GC content: 46.91

Gene sequence:

>1326_bases
ATGTCTGATACAAAACAACCACCATGTGACATAAATGCCGAGCGGGCACTGCTTGGCAGTGTCTTACTCAGTAAAGACGC
AGCGGCAGAGGTATTTGATCGCATAAAGCCAAACCATTTTTACTATGAGGTTCACAAAGAAATATATAAGGTCATGCTTG
ATCTATATGCCGCATCTGAGCCAACCGATATGGTGAGTGTGAGCGAATCTCTTAGGAAAAATCACCGGCTGGAGCACACT
AAGGGCTTTGAGTATCTGAACGGCCTTGCAGAAATGGTTCCCACGCCGGCCAATGTGTACTTCTACGTGAATATCGTTAT
GGAAAAAGCGATTCTCCGTAATCTGGCACAAACAGGGGTGAACATATTCCACATGGCAATGGATGGCGTGCATGATGTGG
AAGAAATTGTCAATAAAGCGCAGAGCGATATTTTCTCAGTAACAACTGATGAAAGTGATGATGATTTGATACGGCTTGAT
GCTGTTGTAAAAGAAACCTGTGAGGGTCTCGATGAATCACATACGAATGAAGATGTTCTGCGCGGTATCGAAACAGGGTT
CACACGCTTTGATTCGCTAACAAACGGGTTGCACCCGGGGCAAATGGTAATCATTGCCGCAAGGCCGTCCATGGGAAAAT
CAACCCTTGCACTTGATTTTGCACGGTCTGCTGCTATAAAACAGCGTGTTCCGACTGTGTTTTTCAGCCTTGAAATGGGC
AGGCAAGATATTGCGATGAGACTTCTGAGCGCAGAAACTGGTATACCCCTGCAAAACATAAGACATGGCAAGTTGTCCCC
CGGTGATTGGGGGGCAATAGGCAGTCTACAAGGTAAATTGGCGGATGTACCATTTTTTATAGATGACTCTCCAAATGTAA
ATCTCGTTGAAATACGTGCAAAGTGTAGACGATTGAAACAGCAGGGGTTGGGGTTCGTAATTATCGACTATCTACAGCTA
CTCTCTGGTAGCCAGAGAGTAGAGACGCGACAACAGGAAGTCAGTGAGTTTTCCAGATCACTAAAACTTTTGGCAAAGGA
GCTGCAGTTGCCGGTATTAGCCCTGTCGCAGCTCAACAGAAGCTCCGAACAGAGAGCCGACAAACGCCCTGCCATGGCTG
ATTTGCGAGAATCAGGCTCAATCGAACAGGATGCGGATCTCGTTATACTGCTCCACCGCGATGAATACTACGACAAAAAT
GCCAGAGCGGGTGAAGGTGATCTTATAATCGCCAAACACCGCAACGGACCGACGGATAATATACCGGTCGCATTTCACGG
CAAATACTCACGCTTCGCTGACCTGCCACGTCAGGACATGCGATAA

Upstream 100 bases:

>100_bases
GTACGCGGCCGATAAAAGGCACGTGGTATGGTGGCATCGACGAATTAGGGCAGCCCATAGAGCCCTTTTTCAGGATCTTT
CCGACTGAGAGAAGCTTTGT

Downstream 100 bases:

>100_bases
ATCAACTTGGGAAAATTTTGAAAAAATCCAGGGAATATATACGCCCACTTGAACTTGTAGGTCTGGCAGGTGTAATGGGA
TTATTTGTTGCATTTACCGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSDTKQPPCDINAERALLGSVLLSKDAAAEVFDRIKPNHFYYEVHKEIYKVMLDLYAASEPTDMVSVSESLRKNHRLEHT
KGFEYLNGLAEMVPTPANVYFYVNIVMEKAILRNLAQTGVNIFHMAMDGVHDVEEIVNKAQSDIFSVTTDESDDDLIRLD
AVVKETCEGLDESHTNEDVLRGIETGFTRFDSLTNGLHPGQMVIIAARPSMGKSTLALDFARSAAIKQRVPTVFFSLEMG
RQDIAMRLLSAETGIPLQNIRHGKLSPGDWGAIGSLQGKLADVPFFIDDSPNVNLVEIRAKCRRLKQQGLGFVIIDYLQL
LSGSQRVETRQQEVSEFSRSLKLLAKELQLPVLALSQLNRSSEQRADKRPAMADLRESGSIEQDADLVILLHRDEYYDKN
ARAGEGDLIIAKHRNGPTDNIPVAFHGKYSRFADLPRQDMR

Sequences:

>Translated_441_residues
MSDTKQPPCDINAERALLGSVLLSKDAAAEVFDRIKPNHFYYEVHKEIYKVMLDLYAASEPTDMVSVSESLRKNHRLEHT
KGFEYLNGLAEMVPTPANVYFYVNIVMEKAILRNLAQTGVNIFHMAMDGVHDVEEIVNKAQSDIFSVTTDESDDDLIRLD
AVVKETCEGLDESHTNEDVLRGIETGFTRFDSLTNGLHPGQMVIIAARPSMGKSTLALDFARSAAIKQRVPTVFFSLEMG
RQDIAMRLLSAETGIPLQNIRHGKLSPGDWGAIGSLQGKLADVPFFIDDSPNVNLVEIRAKCRRLKQQGLGFVIIDYLQL
LSGSQRVETRQQEVSEFSRSLKLLAKELQLPVLALSQLNRSSEQRADKRPAMADLRESGSIEQDADLVILLHRDEYYDKN
ARAGEGDLIIAKHRNGPTDNIPVAFHGKYSRFADLPRQDMR
>Mature_440_residues
SDTKQPPCDINAERALLGSVLLSKDAAAEVFDRIKPNHFYYEVHKEIYKVMLDLYAASEPTDMVSVSESLRKNHRLEHTK
GFEYLNGLAEMVPTPANVYFYVNIVMEKAILRNLAQTGVNIFHMAMDGVHDVEEIVNKAQSDIFSVTTDESDDDLIRLDA
VVKETCEGLDESHTNEDVLRGIETGFTRFDSLTNGLHPGQMVIIAARPSMGKSTLALDFARSAAIKQRVPTVFFSLEMGR
QDIAMRLLSAETGIPLQNIRHGKLSPGDWGAIGSLQGKLADVPFFIDDSPNVNLVEIRAKCRRLKQQGLGFVIIDYLQLL
SGSQRVETRQQEVSEFSRSLKLLAKELQLPVLALSQLNRSSEQRADKRPAMADLRESGSIEQDADLVILLHRDEYYDKNA
RAGEGDLIIAKHRNGPTDNIPVAFHGKYSRFADLPRQDMR

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=38.496583143508, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49386; Mature: 49255

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTKQPPCDINAERALLGSVLLSKDAAAEVFDRIKPNHFYYEVHKEIYKVMLDLYAASE
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PTDMVSVSESLRKNHRLEHTKGFEYLNGLAEMVPTPANVYFYVNIVMEKAILRNLAQTGV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCC
NIFHMAMDGVHDVEEIVNKAQSDIFSVTTDESDDDLIRLDAVVKETCEGLDESHTNEDVL
HHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
RGIETGFTRFDSLTNGLHPGQMVIIAARPSMGKSTLALDFARSAAIKQRVPTVFFSLEMG
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHCC
RQDIAMRLLSAETGIPLQNIRHGKLSPGDWGAIGSLQGKLADVPFFIDDSPNVNLVEIRA
HHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHH
KCRRLKQQGLGFVIIDYLQLLSGSQRVETRQQEVSEFSRSLKLLAKELQLPVLALSQLNR
HHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
SSEQRADKRPAMADLRESGSIEQDADLVILLHRDEYYDKNARAGEGDLIIAKHRNGPTDN
HHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC
IPVAFHGKYSRFADLPRQDMR
CCEEECCCHHHHHCCCHHHCC
>Mature Secondary Structure 
SDTKQPPCDINAERALLGSVLLSKDAAAEVFDRIKPNHFYYEVHKEIYKVMLDLYAASE
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PTDMVSVSESLRKNHRLEHTKGFEYLNGLAEMVPTPANVYFYVNIVMEKAILRNLAQTGV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCC
NIFHMAMDGVHDVEEIVNKAQSDIFSVTTDESDDDLIRLDAVVKETCEGLDESHTNEDVL
HHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
RGIETGFTRFDSLTNGLHPGQMVIIAARPSMGKSTLALDFARSAAIKQRVPTVFFSLEMG
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHCC
RQDIAMRLLSAETGIPLQNIRHGKLSPGDWGAIGSLQGKLADVPFFIDDSPNVNLVEIRA
HHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHH
KCRRLKQQGLGFVIIDYLQLLSGSQRVETRQQEVSEFSRSLKLLAKELQLPVLALSQLNR
HHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
SSEQRADKRPAMADLRESGSIEQDADLVILLHRDEYYDKNARAGEGDLIIAKHRNGPTDN
HHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC
IPVAFHGKYSRFADLPRQDMR
CCEEECCCHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA