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The map label for this gene is hemB [H]

Identifier: 28572885

GI number: 28572885

Start: 840770

End: 841741

Strand: Reverse

Name: hemB [H]

Synonym: TW744

Alternate gene names: 28572885

Gene position: 841741-840770 (Counterclockwise)

Preceding gene: 28572886

Following gene: 28572884

Centisome position: 90.91

GC content: 47.53

Gene sequence:

>972_bases
ATGTTTCAAAAAATACGCACTCGGCGTCTTCGTCTTTCGGAAGCAATGCGTAGATTGGCTTCTGAACATGTTTTGGTCCC
CCATAAACTGGTTCTGCCCATCTTTGTCGGTGAAGGCCTTCAGCCACGACCGATTCCGTCAATGCCCGGGGTTGTGCAAC
ACAGCATTGACAGCTTCAAGCGTGAGCTTCAAAAAGCCGCTTGTGCAAAGCTTGGGGGGGTTATGCTGTTTGGCATTCCT
GATTCTAGATCCGAAGGGGCTGAGGATGCATTCAGGCCGGACGGTATTCTGAATAAGGCTTGCAGTATTGCAGTCCGTGA
GGTTGGAGACTCACTTCTCATACAAACTGATTTGTGCCTTGATGAGTTCACACAGCATGGACATTGCGGCGTTGTCAGCA
AAGATGGATCTATTGATAATGATGCAACATTAGAAATGTACGCTGAGATGGCACTTGCGCAGGCGGCTGCGGGTTCACAA
ATGCTCGGGTTATCTGGATGTATGGATGGGCAAACGGGGTTTGTTCGCGAAGTGCTTGATAGTAACGGAAATCACGATGT
TTCTCTCGTTGCCTACTCTGCTAAGTATGCATCGGCTCTGTATGGTCCATTTAGGCATGCAACAAACTCACCCTTTTCTG
GGAGTCGTAAAACATACCAATTGAATCCTGCCAATTTACGAGAGGCCATTAGAAAAATAGAAGTTGACATTTCTGAGGGC
GCAGATGCATTGATCGTGAAACCTGCAGGGTACTATTTGGATGTCTTACGTTGCGCTTCGGAAATAAGTCGTATTCCTGT
TATGGCGTATCAGGTGTCTGGTGAATATGCAATGATAAAGTCTGCAGCAGCTTGGACTGATACAAAGCAGCTTGTTATAG
AAAGTCTCTTGTCTATACACCGCGCGGGTGCCGATGCAATTCTTACGTACTGGGCGCTGGATGCTGCGTCATGGCTGTCA
GAGACTTTCTAA

Upstream 100 bases:

>100_bases
ATTTGTCAACGCAGCGGGCTGCGAGTTGCTTCAATTGCAAGGACTAAGAATAAAGAGGGTCTTGTACAGGCGCTTTTTGA
ACTGAGTTAGATTGGGTTTA

Downstream 100 bases:

>100_bases
GTTATGCGAACGAATTTTGAATGGTTTGAAGAGGCCAAGCGCTTCATTCCTGGGGGGGTGAATTCACCTGTTAGGGCATA
TGCTGCCGTCGGCGGGACGC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFQKIRTRRLRLSEAMRRLASEHVLVPHKLVLPIFVGEGLQPRPIPSMPGVVQHSIDSFKRELQKAACAKLGGVMLFGIP
DSRSEGAEDAFRPDGILNKACSIAVREVGDSLLIQTDLCLDEFTQHGHCGVVSKDGSIDNDATLEMYAEMALAQAAAGSQ
MLGLSGCMDGQTGFVREVLDSNGNHDVSLVAYSAKYASALYGPFRHATNSPFSGSRKTYQLNPANLREAIRKIEVDISEG
ADALIVKPAGYYLDVLRCASEISRIPVMAYQVSGEYAMIKSAAAWTDTKQLVIESLLSIHRAGADAILTYWALDAASWLS
ETF

Sequences:

>Translated_323_residues
MFQKIRTRRLRLSEAMRRLASEHVLVPHKLVLPIFVGEGLQPRPIPSMPGVVQHSIDSFKRELQKAACAKLGGVMLFGIP
DSRSEGAEDAFRPDGILNKACSIAVREVGDSLLIQTDLCLDEFTQHGHCGVVSKDGSIDNDATLEMYAEMALAQAAAGSQ
MLGLSGCMDGQTGFVREVLDSNGNHDVSLVAYSAKYASALYGPFRHATNSPFSGSRKTYQLNPANLREAIRKIEVDISEG
ADALIVKPAGYYLDVLRCASEISRIPVMAYQVSGEYAMIKSAAAWTDTKQLVIESLLSIHRAGADAILTYWALDAASWLS
ETF
>Mature_323_residues
MFQKIRTRRLRLSEAMRRLASEHVLVPHKLVLPIFVGEGLQPRPIPSMPGVVQHSIDSFKRELQKAACAKLGGVMLFGIP
DSRSEGAEDAFRPDGILNKACSIAVREVGDSLLIQTDLCLDEFTQHGHCGVVSKDGSIDNDATLEMYAEMALAQAAAGSQ
MLGLSGCMDGQTGFVREVLDSNGNHDVSLVAYSAKYASALYGPFRHATNSPFSGSRKTYQLNPANLREAIRKIEVDISEG
ADALIVKPAGYYLDVLRCASEISRIPVMAYQVSGEYAMIKSAAAWTDTKQLVIESLLSIHRAGADAILTYWALDAASWLS
ETF

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=304, Percent_Identity=37.171052631579, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI87081728, Length=314, Percent_Identity=46.1783439490446, Blast_Score=259, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321398, Length=318, Percent_Identity=37.7358490566038, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=37.7551020408163, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35126; Mature: 35126

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKIRTRRLRLSEAMRRLASEHVLVPHKLVLPIFVGEGLQPRPIPSMPGVVQHSIDSFK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RELQKAACAKLGGVMLFGIPDSRSEGAEDAFRPDGILNKACSIAVREVGDSLLIQTDLCL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
DEFTQHGHCGVVSKDGSIDNDATLEMYAEMALAQAAAGSQMLGLSGCMDGQTGFVREVLD
HHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHH
SNGNHDVSLVAYSAKYASALYGPFRHATNSPFSGSRKTYQLNPANLREAIRKIEVDISEG
CCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCC
ADALIVKPAGYYLDVLRCASEISRIPVMAYQVSGEYAMIKSAAAWTDTKQLVIESLLSIH
CCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHCHHHHHHHHHHHHHH
RAGADAILTYWALDAASWLSETF
HCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFQKIRTRRLRLSEAMRRLASEHVLVPHKLVLPIFVGEGLQPRPIPSMPGVVQHSIDSFK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RELQKAACAKLGGVMLFGIPDSRSEGAEDAFRPDGILNKACSIAVREVGDSLLIQTDLCL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
DEFTQHGHCGVVSKDGSIDNDATLEMYAEMALAQAAAGSQMLGLSGCMDGQTGFVREVLD
HHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHH
SNGNHDVSLVAYSAKYASALYGPFRHATNSPFSGSRKTYQLNPANLREAIRKIEVDISEG
CCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCC
ADALIVKPAGYYLDVLRCASEISRIPVMAYQVSGEYAMIKSAAAWTDTKQLVIESLLSIH
CCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHCHHHHHHHHHHHHHH
RAGADAILTYWALDAASWLSETF
HCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]