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The map label for this gene is hemL

Identifier: 28572884

GI number: 28572884

Start: 839366

End: 840766

Strand: Reverse

Name: hemL

Synonym: TW743

Alternate gene names: 28572884

Gene position: 840766-839366 (Counterclockwise)

Preceding gene: 28572885

Following gene: 28572879

Centisome position: 90.8

GC content: 49.32

Gene sequence:

>1401_bases
ATGCGAACGAATTTTGAATGGTTTGAAGAGGCCAAGCGCTTCATTCCTGGGGGGGTGAATTCACCTGTTAGGGCATATGC
TGCCGTCGGCGGGACGCCACGTTTTTTAGCCAAGGCCCAGGGTGCATATGTAACGGATATTGAAGGTAGAGAATATGTTG
ATCTTGTGTCTTCATGGGGTCCGCTTATTCTGGGTCATGCGCACCCCAAGGTGATTGATGCTGTTATAAATACCGCCCAT
AGGGGCATGTCTTACGGAGCGCCAACCACACTTGAAGTAGAGTTAGCAGAGCTTGTGTGCAATCGCATTTGCAATGCGCA
AGGTATCAAGCCGGTTGAACGACTGCGTCTTGTATCAACTGGCACTGAAAGTTGCATGACCGCTATACGACTTGCAAGAT
GTTTCACAGGTCGTGATCTGATCGTTAAGTTTTCCGGACATTATCACGGACATGCTGACCCATTTTTGATAGATGCTGGA
AGTGGTCTTGTGGGTCACCCGTCATCCGGCGGTGTTCCCGAATCTGTTGCAAAAAATACTTTAGTTGTTCCGTACAATGA
CTTGGCTGTTCTTGAATATTTATTTGAAGCTTATCCCAACCAGATTGCATGCATCATAACAGAAGCTTGCCCTGCCAATA
TGGGAGTTATTCCGCCCGATCCGGGTTTTAATGCACGTGTTGCTGACCTTGGGCATTCGCACGGTGCTCTTATTATTTTT
GATGAAGTCATAACCGGTTTTAGAGTTGGATCTGGTGGGTTTTGGGCCCTTGAAACTTCACTTGCTTCGCAACAGCCCAC
CCAAGCCGTTTCAGAAGGGTCTGTTAGCGATTCCGCCTACAGTGCATATGTTCCAGACCTTTTTACCTTTGCAAAGGTCC
TCGGCGGTGGGCTTCCGATTGGAGCTATTGGTGGCCGAGCTGAAATTATGGATTTACTATCCCCATCAGGTCCCGTGTAT
CAGGCAGGTACATTGTCTGGTAACCCGCTTGCAACCGCTGCAGGTCTTGCGACTCTACGCCTGGCAGACTCGAATATCTA
CGAACACATGAATCGGGTAGCACGCGCACTAATAAACGAGATTCAATCTGCTTTTCATGACGCAGGTGTACCATGCACAG
TACAGAGCGCGGGTAATTTGTTCGGCATTTCTTTTAGTGCAATCGCGCCACGGGATTTTACTCAGGCAACAAGTCAAGAA
CACTGGCGATATAGTAACTTTTTTCACTCCATGCTCAACTCGGGTGTTTTATTGCCTCCAAGTGTTTATGAAGCGTGGTT
TGTATCTGCCGCATTCGATGACCGTGCTATTGAAAGGGTCGTAAACGCGCTGCCCGATGCCGTCAGGGCTGCAGGCAGCG
CCCCCGCGCGCCCGAAGGGATTCGAACCCCCAACCTTCTGA

Upstream 100 bases:

>100_bases
TATAGAAAGTCTCTTGTCTATACACCGCGCGGGTGCCGATGCAATTCTTACGTACTGGGCGCTGGATGCTGCGTCATGGC
TGTCAGAGACTTTCTAAGTT

Downstream 100 bases:

>100_bases
TCCGTAGTCAGATGCTCTATCCGTTGAGCTACGGGCGCATAAGATAAGCACAAACAACTCAGCGGAGACGGTGGGATTTG
AACCCACGGTACCCTTTCGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRTNFEWFEEAKRFIPGGVNSPVRAYAAVGGTPRFLAKAQGAYVTDIEGREYVDLVSSWGPLILGHAHPKVIDAVINTAH
RGMSYGAPTTLEVELAELVCNRICNAQGIKPVERLRLVSTGTESCMTAIRLARCFTGRDLIVKFSGHYHGHADPFLIDAG
SGLVGHPSSGGVPESVAKNTLVVPYNDLAVLEYLFEAYPNQIACIITEACPANMGVIPPDPGFNARVADLGHSHGALIIF
DEVITGFRVGSGGFWALETSLASQQPTQAVSEGSVSDSAYSAYVPDLFTFAKVLGGGLPIGAIGGRAEIMDLLSPSGPVY
QAGTLSGNPLATAAGLATLRLADSNIYEHMNRVARALINEIQSAFHDAGVPCTVQSAGNLFGISFSAIAPRDFTQATSQE
HWRYSNFFHSMLNSGVLLPPSVYEAWFVSAAFDDRAIERVVNALPDAVRAAGSAPARPKGFEPPTF

Sequences:

>Translated_466_residues
MRTNFEWFEEAKRFIPGGVNSPVRAYAAVGGTPRFLAKAQGAYVTDIEGREYVDLVSSWGPLILGHAHPKVIDAVINTAH
RGMSYGAPTTLEVELAELVCNRICNAQGIKPVERLRLVSTGTESCMTAIRLARCFTGRDLIVKFSGHYHGHADPFLIDAG
SGLVGHPSSGGVPESVAKNTLVVPYNDLAVLEYLFEAYPNQIACIITEACPANMGVIPPDPGFNARVADLGHSHGALIIF
DEVITGFRVGSGGFWALETSLASQQPTQAVSEGSVSDSAYSAYVPDLFTFAKVLGGGLPIGAIGGRAEIMDLLSPSGPVY
QAGTLSGNPLATAAGLATLRLADSNIYEHMNRVARALINEIQSAFHDAGVPCTVQSAGNLFGISFSAIAPRDFTQATSQE
HWRYSNFFHSMLNSGVLLPPSVYEAWFVSAAFDDRAIERVVNALPDAVRAAGSAPARPKGFEPPTF
>Mature_466_residues
MRTNFEWFEEAKRFIPGGVNSPVRAYAAVGGTPRFLAKAQGAYVTDIEGREYVDLVSSWGPLILGHAHPKVIDAVINTAH
RGMSYGAPTTLEVELAELVCNRICNAQGIKPVERLRLVSTGTESCMTAIRLARCFTGRDLIVKFSGHYHGHADPFLIDAG
SGLVGHPSSGGVPESVAKNTLVVPYNDLAVLEYLFEAYPNQIACIITEACPANMGVIPPDPGFNARVADLGHSHGALIIF
DEVITGFRVGSGGFWALETSLASQQPTQAVSEGSVSDSAYSAYVPDLFTFAKVLGGGLPIGAIGGRAEIMDLLSPSGPVY
QAGTLSGNPLATAAGLATLRLADSNIYEHMNRVARALINEIQSAFHDAGVPCTVQSAGNLFGISFSAIAPRDFTQATSQE
HWRYSNFFHSMLNSGVLLPPSVYEAWFVSAAFDDRAIERVVNALPDAVRAAGSAPARPKGFEPPTF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=342, Percent_Identity=26.9005847953216, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24119277, Length=348, Percent_Identity=24.7126436781609, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=213, Percent_Identity=28.6384976525822, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786349, Length=447, Percent_Identity=45.413870246085, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789016, Length=374, Percent_Identity=29.9465240641711, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1787560, Length=377, Percent_Identity=28.6472148541114, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789759, Length=431, Percent_Identity=24.8259860788863, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788044, Length=359, Percent_Identity=26.4623955431755, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693181, Length=355, Percent_Identity=26.1971830985915, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71992977, Length=353, Percent_Identity=26.628895184136, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=351, Percent_Identity=23.9316239316239, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356575, Length=390, Percent_Identity=26.4102564102564, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_TROW8 (Q83H98)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789664.1
- ProteinModelPortal:   Q83H98
- SMR:   Q83H98
- STRING:   Q83H98
- GeneID:   1064943
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW743
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   Q83H98
- ProtClustDB:   PRK00062
- BioCyc:   TWHI218496:TW0709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 49583; Mature: 49583

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNFEWFEEAKRFIPGGVNSPVRAYAAVGGTPRFLAKAQGAYVTDIEGREYVDLVSSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHCCCCEEEECCCCHHHHHHHCCC
PLILGHAHPKVIDAVINTAHRGMSYGAPTTLEVELAELVCNRICNAQGIKPVERLRLVST
CEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GTESCMTAIRLARCFTGRDLIVKFSGHYHGHADPFLIDAGSGLVGHPSSGGVPESVAKNT
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCE
LVVPYNDLAVLEYLFEAYPNQIACIITEACPANMGVIPPDPGFNARVADLGHSHGALIIF
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEE
DEVITGFRVGSGGFWALETSLASQQPTQAVSEGSVSDSAYSAYVPDLFTFAKVLGGGLPI
HHHHCCEEECCCCEEEEEHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GAIGGRAEIMDLLSPSGPVYQAGTLSGNPLATAAGLATLRLADSNIYEHMNRVARALINE
CCCCCHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHH
IQSAFHDAGVPCTVQSAGNLFGISFSAIAPRDFTQATSQEHWRYSNFFHSMLNSGVLLPP
HHHHHHHCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCC
SVYEAWFVSAAFDDRAIERVVNALPDAVRAAGSAPARPKGFEPPTF
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTNFEWFEEAKRFIPGGVNSPVRAYAAVGGTPRFLAKAQGAYVTDIEGREYVDLVSSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHCCCCEEEECCCCHHHHHHHCCC
PLILGHAHPKVIDAVINTAHRGMSYGAPTTLEVELAELVCNRICNAQGIKPVERLRLVST
CEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GTESCMTAIRLARCFTGRDLIVKFSGHYHGHADPFLIDAGSGLVGHPSSGGVPESVAKNT
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCE
LVVPYNDLAVLEYLFEAYPNQIACIITEACPANMGVIPPDPGFNARVADLGHSHGALIIF
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEE
DEVITGFRVGSGGFWALETSLASQQPTQAVSEGSVSDSAYSAYVPDLFTFAKVLGGGLPI
HHHHCCEEECCCCEEEEEHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GAIGGRAEIMDLLSPSGPVYQAGTLSGNPLATAAGLATLRLADSNIYEHMNRVARALINE
CCCCCHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHH
IQSAFHDAGVPCTVQSAGNLFGISFSAIAPRDFTQATSQEHWRYSNFFHSMLNSGVLLPP
HHHHHHHCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCC
SVYEAWFVSAAFDDRAIERVVNALPDAVRAAGSAPARPKGFEPPTF
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174