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The map label for this gene is lysC [H]

Identifier: 28572866

GI number: 28572866

Start: 820137

End: 821393

Strand: Reverse

Name: lysC [H]

Synonym: TW725

Alternate gene names: 28572866

Gene position: 821393-820137 (Counterclockwise)

Preceding gene: 28572867

Following gene: 28572865

Centisome position: 88.71

GC content: 46.62

Gene sequence:

>1257_bases
TTGAGCCTGATAGTTCAGAAATACGGCGGGTCATCGCTTGCCAGTGCCAATAACCTCAAGAGAGTTGCCGAACGTGTTTC
TCGTGCGTATTACGGTAACAATCGGCTGGTTGTTGTAGTATCTGCGATGGGGAGTACTACCGATAGGCTTCTAACTCTTG
CTCATGAAGTTTCTGTTGAGCCAGAGAGACGTGAGTTGGATGTCTTACTCTCAACGGGTGAATGTGTGACGGCTTCGCTT
CTGGCGATGGCCATAAACTCTATTGGCCTTCCTGCCCGATCTTTTACGGCCGCTCAGGCTGGGATTGTAACAGATGAGCA
TCATTGTAATGCGCGAATTATCACCATAACACCTACCCTGATAGAAGATGCGCTTAACGCAGGGGAGATTGCGGTTGTTG
CTGGCTTCCAAGGCGTTAATCGAAAAGGTCAGGTAACCACCCTTGGTCGTGGGGGGTCAGATACGACTGCCGTGGCTCTA
GCAGCTGCACTGAAAGCTGACAGATGTGAGATATATTCTGATGTCGAGGGGGTATTTACAGCAGATCCGAGAATAGTGCC
TAAGGCACGGTTGCTCGAATCAATACCAATTGACCATATGCTCGAGCTATCAACTGCTGGTGCACGTATTTTAAGTTTAC
GTTCAGTCGTTTTTGCAAAGAGACACAATGTTTCAGTCAAGGTTGGATCAACGTTTTCTGATACATCCGGAACAATACTG
TGTAACGAAGGAGAAGCAATGGAAGAGCCTGTTATAACCGGTATTGCAACAGACACAAGTCAGGCAAGAATTACGATTGT
TGGCTTGCCTGACATTCCAGGAAGTGCAGCTGAGGTATTCTCTGCTCTGTCTGTTGCAGAGGTGGCAGTGGATAACATAG
TACAGAACTTTTCCTCAAGTAATACAGGTTTTATAGACTTATCCTTTACATCACCTAAGGCCCAAGGTCCAGCGGTTACG
AGCGCCCTTCGAGAAAATCAGACGCGAATCGGGTATAAGGATCTTCAGTACGATGATGAAGTTGGCAAAATAACCGTTGT
GGGTTCTGGAATTCAACATTCTGCTGATATATCCGCTCAGCTCTTTCGGTTATTATCTGATGCCAAAGTAAATGTAGAAA
TGATATCTACCAGTGAGATGCGAATCTCTTTTGTTGTAAGGGCCAATGTTGTAGCAGAGGTTGCTCGTTTAATTCACACT
TCCTTTGGCCTCGATGCTGACCTGCGTGCAGTTGTGCACTCTGAAGGCCGTGTATGA

Upstream 100 bases:

>100_bases
GGTGTGCGGGGGTCTTGCGGGCTCCGTTCGAACGCATCGCTGTTCAGATTTTGTTTTATTAACAAGTCGGACTTTAAGAT
CCTTGTAGTTTGGAGAACTG

Downstream 100 bases:

>100_bases
CATCGCTGGCAATTATCGGGGCTACGGGGCAGGTTGGTATCATCCTGCGCTCACTTATCGTCGAAAGGCATTTTCCGGTT
GATTCAATCCGCTTCTTTGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSLIVQKYGGSSLASANNLKRVAERVSRAYYGNNRLVVVVSAMGSTTDRLLTLAHEVSVEPERRELDVLLSTGECVTASL
LAMAINSIGLPARSFTAAQAGIVTDEHHCNARIITITPTLIEDALNAGEIAVVAGFQGVNRKGQVTTLGRGGSDTTAVAL
AAALKADRCEIYSDVEGVFTADPRIVPKARLLESIPIDHMLELSTAGARILSLRSVVFAKRHNVSVKVGSTFSDTSGTIL
CNEGEAMEEPVITGIATDTSQARITIVGLPDIPGSAAEVFSALSVAEVAVDNIVQNFSSSNTGFIDLSFTSPKAQGPAVT
SALRENQTRIGYKDLQYDDEVGKITVVGSGIQHSADISAQLFRLLSDAKVNVEMISTSEMRISFVVRANVVAEVARLIHT
SFGLDADLRAVVHSEGRV

Sequences:

>Translated_418_residues
MSLIVQKYGGSSLASANNLKRVAERVSRAYYGNNRLVVVVSAMGSTTDRLLTLAHEVSVEPERRELDVLLSTGECVTASL
LAMAINSIGLPARSFTAAQAGIVTDEHHCNARIITITPTLIEDALNAGEIAVVAGFQGVNRKGQVTTLGRGGSDTTAVAL
AAALKADRCEIYSDVEGVFTADPRIVPKARLLESIPIDHMLELSTAGARILSLRSVVFAKRHNVSVKVGSTFSDTSGTIL
CNEGEAMEEPVITGIATDTSQARITIVGLPDIPGSAAEVFSALSVAEVAVDNIVQNFSSSNTGFIDLSFTSPKAQGPAVT
SALRENQTRIGYKDLQYDDEVGKITVVGSGIQHSADISAQLFRLLSDAKVNVEMISTSEMRISFVVRANVVAEVARLIHT
SFGLDADLRAVVHSEGRV
>Mature_417_residues
SLIVQKYGGSSLASANNLKRVAERVSRAYYGNNRLVVVVSAMGSTTDRLLTLAHEVSVEPERRELDVLLSTGECVTASLL
AMAINSIGLPARSFTAAQAGIVTDEHHCNARIITITPTLIEDALNAGEIAVVAGFQGVNRKGQVTTLGRGGSDTTAVALA
AALKADRCEIYSDVEGVFTADPRIVPKARLLESIPIDHMLELSTAGARILSLRSVVFAKRHNVSVKVGSTFSDTSGTILC
NEGEAMEEPVITGIATDTSQARITIVGLPDIPGSAAEVFSALSVAEVAVDNIVQNFSSSNTGFIDLSFTSPKAQGPAVTS
ALRENQTRIGYKDLQYDDEVGKITVVGSGIQHSADISAQLFRLLSDAKVNVEMISTSEMRISFVVRANVVAEVARLIHTS
FGLDADLRAVVHSEGRV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=281, Percent_Identity=32.7402135231317, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790455, Length=172, Percent_Identity=38.3720930232558, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1790376, Length=286, Percent_Identity=26.5734265734266, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=370, Percent_Identity=22.972972972973, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44253; Mature: 44122

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00626 RCC1_2 ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIVQKYGGSSLASANNLKRVAERVSRAYYGNNRLVVVVSAMGSTTDRLLTLAHEVSVE
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCC
PERRELDVLLSTGECVTASLLAMAINSIGLPARSFTAAQAGIVTDEHHCNARIITITPTL
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEECHHH
IEDALNAGEIAVVAGFQGVNRKGQVTTLGRGGSDTTAVALAAALKADRCEIYSDVEGVFT
HHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCEEE
ADPRIVPKARLLESIPIDHMLELSTAGARILSLRSVVFAKRHNVSVKVGSTFSDTSGTIL
CCCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCEECCCCCEEE
CNEGEAMEEPVITGIATDTSQARITIVGLPDIPGSAAEVFSALSVAEVAVDNIVQNFSSS
ECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NTGFIDLSFTSPKAQGPAVTSALRENQTRIGYKDLQYDDEVGKITVVGSGIQHSADISAQ
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHH
LFRLLSDAKVNVEMISTSEMRISFVVRANVVAEVARLIHTSFGLDADLRAVVHSEGRV
HHHHHHCCCEEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SLIVQKYGGSSLASANNLKRVAERVSRAYYGNNRLVVVVSAMGSTTDRLLTLAHEVSVE
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCC
PERRELDVLLSTGECVTASLLAMAINSIGLPARSFTAAQAGIVTDEHHCNARIITITPTL
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCEEEEEECHHH
IEDALNAGEIAVVAGFQGVNRKGQVTTLGRGGSDTTAVALAAALKADRCEIYSDVEGVFT
HHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCEEE
ADPRIVPKARLLESIPIDHMLELSTAGARILSLRSVVFAKRHNVSVKVGSTFSDTSGTIL
CCCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCEECCCCCEEE
CNEGEAMEEPVITGIATDTSQARITIVGLPDIPGSAAEVFSALSVAEVAVDNIVQNFSSS
ECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NTGFIDLSFTSPKAQGPAVTSALRENQTRIGYKDLQYDDEVGKITVVGSGIQHSADISAQ
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHH
LFRLLSDAKVNVEMISTSEMRISFVVRANVVAEVARLIHTSFGLDADLRAVVHSEGRV
HHHHHHCCCEEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]