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The map label for this gene is ribH

Identifier: 28572851

GI number: 28572851

Start: 803253

End: 803723

Strand: Direct

Name: ribH

Synonym: TW710

Alternate gene names: 28572851

Gene position: 803253-803723 (Clockwise)

Preceding gene: 28572850

Following gene: 28572863

Centisome position: 86.75

GC content: 46.5

Gene sequence:

>471_bases
ATGGGTAAAGACAGAAATCTCAGCCTAAATTCCCCAGAAAGTATACAGAGTGTGATTGGGGATACGGATATTAAAATAGC
TGTTATTGCAGCAATGTGGCATGAGAAAATAGTTAGAGGTCTTATAGATGGTGCAACGAGAGAAATTGTTTCCAGTGGAT
CTCATGCAGAACTGTTTAGAGTGCCCGGCAGTGTTGAGCTTGTCCTTGCGTGTAAAAAACTACTAAAAACATTTGATGCC
GCAGTCGCGCTCGGTGTGATCTTGCGGGGAGAAACTGATCATAATACGCATATAGCGCGGTTAGTGTTGAGCGGTATAAC
CGATGTTATGCTCGCCCTGGAGAAGCCTATTGGGATCGGAGTCTTGACAATCGATACCGAGCAGCAGGGTCTTGACAGGT
CAAGTGGTGACTACAATGCGGGGGTCAATGCGGCAAGGGCTGTCATTAGGATGGTCAGGTTGTTTCGGTAG

Upstream 100 bases:

>100_bases
AAGTTGGGCTTGGTAAATGCAATCATGAATACCTTAAAGTAAAACGCGACAAAATGGGTCATAAAATCGAGTTATAGCAT
TCTGTGCAGAATGAGTAGAA

Downstream 100 bases:

>100_bases
TGCCATCGCGGTGTAACAATCTGCATTATTGCGGTCGCTGCGGGGCGTTTTCTGGCACGGGGTAGTGATATGTCATGTGT
GATAACTATTGACAATGTGC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MGKDRNLSLNSPESIQSVIGDTDIKIAVIAAMWHEKIVRGLIDGATREIVSSGSHAELFRVPGSVELVLACKKLLKTFDA
AVALGVILRGETDHNTHIARLVLSGITDVMLALEKPIGIGVLTIDTEQQGLDRSSGDYNAGVNAARAVIRMVRLFR

Sequences:

>Translated_156_residues
MGKDRNLSLNSPESIQSVIGDTDIKIAVIAAMWHEKIVRGLIDGATREIVSSGSHAELFRVPGSVELVLACKKLLKTFDA
AVALGVILRGETDHNTHIARLVLSGITDVMLALEKPIGIGVLTIDTEQQGLDRSSGDYNAGVNAARAVIRMVRLFR
>Mature_155_residues
GKDRNLSLNSPESIQSVIGDTDIKIAVIAAMWHEKIVRGLIDGATREIVSSGSHAELFRVPGSVELVLACKKLLKTFDAA
VALGVILRGETDHNTHIARLVLSGITDVMLALEKPIGIGVLTIDTEQQGLDRSSGDYNAGVNAARAVIRMVRLFR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=149, Percent_Identity=29.5302013422819, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_TROW8 (Q83HB9)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789631.1
- ProteinModelPortal:   Q83HB9
- SMR:   Q83HB9
- STRING:   Q83HB9
- GeneID:   1064910
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW710
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   CLSK230230
- BioCyc:   TWHI218496:TW0676-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16771; Mature: 16640

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKDRNLSLNSPESIQSVIGDTDIKIAVIAAMWHEKIVRGLIDGATREIVSSGSHAELFR
CCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VPGSVELVLACKKLLKTFDAAVALGVILRGETDHNTHIARLVLSGITDVMLALEKPIGIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VLTIDTEQQGLDRSSGDYNAGVNAARAVIRMVRLFR
EEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GKDRNLSLNSPESIQSVIGDTDIKIAVIAAMWHEKIVRGLIDGATREIVSSGSHAELFR
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VPGSVELVLACKKLLKTFDAAVALGVILRGETDHNTHIARLVLSGITDVMLALEKPIGIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VLTIDTEQQGLDRSSGDYNAGVNAARAVIRMVRLFR
EEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174